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All Journal International Journal of Public Health Science (IJPHS) Jurnal Surya Medika Acta Biochimica Indonesiana Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat JPP Jurnal Kesehatan Poltekkes Palembang Jurnal Integrasi Kesehatan dan Sains Indonesian Journal of Global Health research Jurnal Perak Malahayati : Pengabdian Kepada Masyarakat Jurnal Pengabdian kepada Masyarakat (ABDIKEMAS)
Ocktariyana, Ocktariyana
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Religion Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : JPP Jurnal Kesehatan Poltekkes Palembang

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ANALISIS KEMURNIAN EKSTRAKSI ASAM RIBONUKLEAT PADA SAMPEL DARAH MENSTRUASI Ocktariyana Ocktariyana; Dian Lestari; Asmarinah Asmarinah
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 17 No 2 (2022): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : POLITEKNIK KESEHATAN KEMENKES PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v17i2.1392

Abstract

Latar Belakang: Asam nukleat mengandung materi genetik dan berfungsi untuk mengatur perkembangan biologis seluruh bentuk kehidupan secara seluler. Asam nukleat yang paling umum adalah Asam deoksiribonukleat (ADN) dan Asam ribonukleat (ARN). Untuk mengeluarkan ARN dari dalam intisel maka diperlukan suatu teknik isolasi. Suatu ekstraksi asam nukleat dikatakan baik jika dari prosedur yang dilakukan bisa didapatkan asam nukleat yang murni dan utuh. Penelitian ini bertujuan untuk mengetahui bagaimana tingkat kemurnian ekstrak asam nukleat dari sampel darah menstruasi menggunakan teknik isolasi asam nukleat. Metode: Sampel darah menstruasi dikumpulkan dengan cara ditampung pada kertas saring yang di desain khusus. Sampel akan diekstraksi menggunakan Quick-ARN Miniprep Plus Kit R1058 Zymo Research untuk isolasi ARN, selanjutnya diukur tingkat kemurnian dengan menggunakan alat nanodrop berdasarkan prinsip spektrofotometri. Data diolah secara statistic dengan menggunakan analisis deskripsi dalam disajikan dalam bentu distribusi frekuensi dan nilai rerata. Hasil: pada penelitian ini diperoleh bahwa rerata tangka kemurnian ARN sampel darah menstruasi yang ditampung pada kertas saring pada Panjang gelombang A260 / A280 adalah 2,07, dan Panjang gelombang A260 / A230 adalah 2,1. Kesimpulan: Isolasi ARN pada sampel darah menstruasi yang ditampung di kertas saring memiliki tingkat kemurnian yang optimal.
PENGARUH PEMBERIAN EKSTRAK ISOFLAVON TEMPE TERHADAP TINGKAT STRESS OKSIDATIF PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus novergicus) SETELAH DIINDUKSI DENGAN DOSIS TOKSIK PARASETAMOL Muhamad Taswin; Ratnaningsih Dewi Astuti; Dewi Marlina; Ocktariyana Ocktariyana; Ade Agustianingsih
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 17 No 2 (2022): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : POLITEKNIK KESEHATAN KEMENKES PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v17i2.1513

Abstract

ABSTRACT Background: As a producing country and at the same time the largest consumer of tempeh in the world, Indonesia has become the largest soybean market in Asia. As much as 50% of soybean consumption in Indonesia is done in the form of tempeh. Tempe is a potential food ingredient as a hepatoprotector. The content of isoflavones in tempeh has been shown to protect rat liver function under stress conditions. Isoflavones contained in tempeh have activity as antioxidants, which can prevent oxidation reactions from occurring by working as reducing agents and protecting cell membranes from oxidation, as well as counteracting free radicals by stopping chain reactions and protecting cells from DNA activation so as to reduce cell damage. This situation has prompted the authors to investigate the effect of the isoflavone extract of tempeh on the histochemistry and histopathology of the livers of male white Wistar rats (Rattus novergicus) after being induced by toxic doses of Paracetamol. Methods: This study was an experimental study using the Randomized Posttest Only Control Group Design to determine the level of oxidative stress after administration of tempeh isoflavone extract to male white rats (Rattus novergicus) induced with a toxic dose of paracetamol. 5 groups, namely group 1 was given tempe extract 160 mg/kgBB, group 2 was given tempe extract 320 mg/kgBB, group 3 was given tempe extract 640 mg/kgBB, group 5 was given distilled water and group 6 was given 1% NaCMC solution. Each rat was treated for 14 days. On day 12, 13 and 14 rats were given paracetamol at a dose of 900 mg/kg BW in 1% NaCMC. And on the 15th day, rat blood serum was examined for levels of SGOT, SGPT and MDA. Results: It was found that tempeh at a dose of 640 mg/kgBW could significantly reduce SGOT, SGPT and MDA levels (p<0.05) compared to other doses as well as distilled water and 1% NaCMC. Conclusion: So it can be concluded that tempeh can be an alternative source of antioxidants that can protect liver cells from the effects of substances that can damage the liver. Keywords: Tempe, SGOT, SGPT, MDA
Co-Authors Abdul Kadir Ade Agustianingsih Ani Lasepha Apriliani Apriliani Aprilina Aprilina Aprilina Aprilina Asmarinah Asmarinah Dewi Marlina DIAN LESTARI Dian Lestari Dian Lestari Fadillah Sari Handayani Handayani Heni Sumastri Indah Puji Septeria Indah Yuliana Listrianah Listrianah Maria Eka Yuliastuti Muhamad Taswin Murdiningsih Murdiningsih Ratnaningsih Dewi Astuti Risnawati Tanung Rizma Adlia Syakurah Rohaya Rohaya Rostika Flora Rostika Flora St Hindun Zulkarnain Zulkarnain