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Journal : Indonesian Journal of Biotechnology

Early development of self‐administered COVID‐19 rapid test based on nucleocapsid detection in saliva sample Siti Soidah; Toto Subroto; Sari Syahruni; Fauzian Giansyah; Henry Chandra; Dhiya Salsabila; Bachti Alisjahbana; Nisa Fauziah; Hesti Lina Wiraswati; Leonardus Wiydatmoko; Basti Andriyoko; Anita Yuwita; Muhammad Yusuf
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.72269

Abstract

More than 6,000,000 people have died due to the coronavirus (COVID‐19) pandemic. This disease spread quickly due to its highly contagious nature. The SARS‐CoV‐2 virus that causes the disease can be transmitted through saliva droplets secreted by infected people at a distance of less than 1 m. As a result, saliva has been accepted as an alternative specimen for COVID‐19 detection by the Centers for Disease Control and Prevention (CDC). Furthermore, WHO recommended the use of rapid antigen tests based on lateral flow immunoassay when reverse transcription‐polymerase chain reaction (RT‐PCR) is not available. We developed a saliva‐based rapid antigen test by optimizing the antibody concentration and optimum pH for the conjugation of antibody and gold nanoparticles. We found that the best running buffer formulation consisted of 75 mM sodium phosphate buffer, 1% NaCl, 1% Triton X‐100, 0.5% N‐acetyl‐L‐cysteine, and 0.02% sodium azide. The addition of a mucolytic agent in the buffer can reduce the viscosity of saliva, thus improving sensitivity. The rapid test developed detected the lowest concentration of nucleocapsid protein at 0.1 μg/mL. Our study revealed 100% specificity against negative COVID‐19 saliva and no cross‐reaction with avian influenza virus hemagglutinin.
Co-Authors Afandi, Ressa Novita Agnes Rengga Indrati Ahmad Sulaeman Amaylia Oehadian Andre van der Venn Angèle JGMGerver-Jansen Angky Tririni Anita Yuwita Anna Alisjahbana Ardini S. Raksanagara Ardini S. Raksanagara, Ardini S. Basti Andriyoko Budiman , Budiman Clara M Kusharto Dewi Kartika Turbawaty Dhiya Salsabila Dick van Soolingen Dida Ahmad Gurnida Dwi Agustian Edhyana Sahiratmadja Endang Sutedja Eva Mardiana Hidayat Evan Susandi Fauzian Giansyah Febriani, Ulfah Dwi Gumilang, Manik Intan Guntur Darmawan Hadyana Sukandar Hendra Subroto, Hendra Henriette A Delemarre–van de Waal Henry Chandra Herry Garna Hesti Lina Wiraswati Hidayat, Eva Mardiana Hikmat Permana Hinta Meijerink Hofiyah Djauhari Ida Parwati Ida Parwati Irvan Afriandi Irvan Afriandi Jeffery Malachi Candra Jose Batubara Juhariah Juhariah Julia Hartati Koesoemadinata, Raspati Cundarani Krishna Yana Krisnian, Tharani Kumia Wahyudi Kusumawardhani, R.N. Yasmin Lelly Yuniarti Leonardus Wiydatmoko marni banowati Menik Herdwiyanti Muhammad Yusuf Nanan Sekarwana Nanny Natalia Mulyani Soetedjo Ni Sayu Dewi B. Nisa Fauziah Novi Jayanti Nuni Sulastri Nurizzatun Nafsi Nurul Hidayah Chairunnisa Nury Fitria Dewi obin sarwita Panji Fortuna Hadisoemarto Parse, Rocci Jack Prayudi Santoso Putri Vidyaniati Raspati Cundarani Koesoemadinata Reinout van Crevel Reinout van Crevel Ressa Novita Afandi Resvi Livia Rocci Jack Parse Rovina Ruslami Rovina Ruslami, Rovina Santoso, Ida Parwati Sari Syahruni Siti Soidah Sofia Imaculata Suparman Hardinsyah, Suparman Susan Margaret McAllister Susantina Prodjosoewojo Syndi Nurmawati Teow Sheng Hao Tharani Krisnian Tjahjani M. Sudiro Tony Sadjimin Toto Subroto Trinugroho Heri Fadjari, Trinugroho Heri Tya Listiaty Ulfah Dwi Febriani Uun Sumardi, Uun Willem J Gerver Wirawan, Chevie Woro Indri Padmosiwi Yana, Krishna Yani Triyani Yani Triyani Yanti Mulyana Yeva Rosana Yovita Hartantri Yulniar Tasli