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Gede Suastika

Institut Pertanian Bogor

Author-ID : 2451905

Medicine & Pharmacology

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Identifikasi Meloidogyne Penyebab Penyakit Umbi Bercabang pada Wortel di Dataran Tinggi Dieng Muhamad Taher; Supramana .; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Branched tuber disease is a new problem on carrot cultivation in Indonesia. The disease may reduce the quantity and quality of the tubers and causing significant yield losses. Meloidogyne spp. has been identified as the primary cause of branched or forked disease of carrot in West Java. Similar disease was observed in carrot field in Dieng Plateau, Central Java. The study was conducted at three locations in the Dieng Plateau having different elevation. Nematode species identification was done by observing the perinneal pattern of 150 samples of female nematode and obtained four Meloidogyne species, namely M. arenaria, M. hapla, M. incognita, and M. javanica. All four species were found associated with branched tuber disease in three locations with M. incognita as a dominant species having prevalence levels of 50% to 58%.Key words: branched tuber disease, carrots, Meloidogyne, species identification

Identifikasi Spesies Meloidogyne spp. Penyebab Umbi Bercabang pada Tanaman Wortel di Jawa Timur Zalzilatul Hikmia Hikmia; Supramana .; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 8 No. 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Branched tuber is a new disease in carrot cultivation in Indonesia and has caused big losses for farmers. In 2010, several species of root-knot nematodes, Meloidogyne spp., were reported as the primary cause of disease and the yield loss due to the disease in the region of Agropolitan-Cianjur, West Java, reached 15% to 95%. Symptoms of tuber branching has also been reported in one of the centers of vegetable production in East Java, in the subdistrict of Bumiaji, Kota Batu. Research aimed at identifying Meloidogyne species on carrot was carried out on carrot plantation. The study consisted of two activities, i.e. surveillance and identification. Surveillance was carried out at three different altitudes/elevations, i.e. 1600 m, 1700 m and 1800 m asl (above sea level). Identification was done by PCR ITS r-DNA using multiplex primer for mix spesies identification of M. hapla, M. fallax, and M. chitwoodi and the spesific primer for identification of M. arenaria, M. incognita, and M. javanica. Identification based on ITS r-NA by PCR gave positive results for four species, namely M. hapla, M. arenaria, M. incognita, and M. javanica.Key words: branched tuber disease, carrot, Meloidogyne, PCR ITS r-DNA

Laporan Pertama tentang Infeksi Polerovirus pada Tanaman Cabai di Daerah Bali, Indonesia Gede Suastika; Sedyo Hartono; I Dewa Nyoman Nyana; Tomohide Natsuaki
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

During a visit to Bali Island in September 2011, we found chilipepper plants exhibiting yellowing symptoms. The infected plants showed quite similar symptom to those of the recently reported Pepper yellow leaf curl virus from Israel and Japan, but there was no shortening of internodes or leaf rolling. We have ampliﬁed part of the genome of a virus associated with this disease using degenerate primers for members of the genus Polerovirus. The reverse transcription-polymerase chain reaction (RT-PCR) from symptom-bearing samples resulted in the amplification of a 650 bp band which is the expected size. RT-PCR from healthy samples did not produce an amplicon. Based on our results, we concluded that yellowing disease on chilipepper observed in Payangan, Gianyar, Bali is associated with a virus belonging to the genus Polerovirus. This is the first report on Polerovirus infection in chilipepper in Indonesia. Some researches for elucidation of the virus species and biological/molecular characters are being conducted.Key word: chilipepper, Indonesia, Polerovirus

Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida Halimah Halimah; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root-knot nematodes (RKN), Meloidogyne spp., were reported as the cause of carrot branched tuber on several vegetable production areas in Central and East Java. Species identification by molecular approach was conducted using infected carrot tubers from Agropolitan Cianjur, West Java. DNA was extracted from female nematodes and amplified using PCR with species specific primers (Fjav/Rjav for M. javanica, Far/Rar for M. arenaria, and Finc/Rinc for M. incognita) and multiplex primer (M. hapla, M. chitwoodi, and M. fallax). PCR product were sequenced without cloning. Based on nucleotide sequences, two species RKN were found associated with branched tuber disease of carrot in Agropolitan Cianjur, i.e M. javanica and M. hapla. Phylogenetic analysis showed that M. javanica from Cianjur was closely related to RKN from China with the homology level of 91.9%, whereas nucleotide sequence of M. hapla from Cianjur had high homology level (99.4% to 100%) with isolates from Swiss, USA, UK, and China.Key words: Meloidogyne hapla, M. javanica, phylogenetic, root-knot nematode

Deteksi dan Identifikasi Virus pada Umbi Bawang Merah Arif Kurniawan; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 2 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Shallot is an important horticultural crop in Indonesia. One of it’s production constraint is viral disease. Dispersal of viruses on shallots may occurre through shallots bulb trading. However, there is not much information regarding viruses infecting shallot bulbs. Laboratory study was conducted to detect and identify viruses associated with shallot. Random sampling was done for several varieties of shallots (Jawa, Biru, Nganjuk, and Brebes varieties) collected from Bantul Districs. Virus detection and identification was based on RT-PCR using specific primer of OYDV, SYSV, SLV, and ShVX followed by sequencing of the nucleotides. RT-PCR was only successfully amplified Shallot yellow stripe virus (SYSV) with size ~749 bp, while other viruses were not detected. Disease incidence of SYSV on Java and Brebes varieties were 60% and 53.3% respectively. Nucleotide sequences of SYSV CP gene showed the highest homology to SYSV from China and South Korea.Key words: RT-PCR, sequencing nucleotides, Shallot yellow stripe virus

Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using reverse transcription polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.

Penggunaan Galur Lemah Chili veinal mottle virus untuk Proteksi Silang Asniwita Asniwita; Sri Hendrastuti Hidayat; Gede Suastika; Slamet Susanto; Sriani Sujiprihati
Jurnal Fitopatologi Indonesia Vol 9 No 5 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Inoculation of mild virus strain prior to severe virus strain to protect plant against viral disease is the principle of cross protection. Five mild strains of Chili veinal mottle virus (ChiVMV), i.e. -KAR, -SPR, -SKT, -CSR, and -PGL were used as cross protection agent to protect chili pepper plants against severe strain infection of ChiVMV-CKB. The mild strains were inoculated mechanically prior inoculation of severe strain and the efficiency of cross protection was evaluated by observing symptom development and measuring crop yield. Inoculation of mild strains 7 days prior inoculation of severe stain was not able to protect the plant from infection of severe strain ChiVMV-CKB. Protective effect was observed when mild strains were inoculated at 14, 21, and 28 days prior inoculation of severe strain. Symptom development was suppressed or delayed, and crop yield was not significantly different with healthy plants. It was suggested that to obtain the best protection against severe strain, the mild strain should be applied as early as possible before the occurrence of severe strain infection.

Deteksi dan Identifikasi Potyvirus pada Ubi Jalar di Tana Toraja, Sulawesi Selatan Laras Anjarsari; Gede Suastika; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Typical viral symptoms including chlorotic and uneven interveinal yellowing on leaves without leaf malformation was observed on sweet potato field in Tana Toraja, South Sulawesi. To identify the causal of the disease, reverse transcription-polymerase chain reaction (RT-PCR) and DNA sequencing were carried out to detect the virus from infected plants. RT-PCR using universal primer for C1 gene of Potyvirus was successfully amplified approximately 683bp DNA fragment. The nucleotide sequences of this C1 gene fragment showed 98% homology to Sweet potato virus G (SPVG). Amplification using specific primer for coat protein (CP) gene of SPVG followed by DNA sequencing confirmed the association of SPVG from infected plants. Further nucleotide analysis shwowed that SPVG isolate from Tana Toraja had 99.2% homology to isolate from Japan. This is the firstt report of SPVG infection on sweet potato in South Sulawesi.

Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Rizki Haerunisa; Erniawati Diningsih; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 10 No 3 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Mottle disease of carnation (Dianthus caryophyllus) was observed in cultivation areas in Cianjur and Bandung, West Java. This study aimed to identify the virus causing leaf mottling on carnation using reverse transcription-polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. Fragment DNA of 1000 bp was successfully amplified using specific primers for coat protein of Carmovirus (BC57/BC58). Direct sequencing was proceeded using PCR products. Nucleotide sequence analysis confirmed the infection of Carnation mottle virus (CarMV) from leaves showing mottle symptom. Furthermore, phylogenetic analysis showed that CarMV isolates from Indonesia have close relationship with those from Brazil, Israel, Iran, India, Netherland, and Spain.

Deteksi dan Identifikasi Spesies Meloidogyne pada Tanaman Wortel dari Dataran Tinggi Malino, Gowa, Sulawesi Selatan Hishar Mirsam; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 1 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Meloidogyne spp. was reported as the cause of branched tuber disease on several carrot production areas in Java, Indonesia and may potentially cause yield loss. This research aimed to use morphological and molecular characters to detect and identify Meloidogyne species on carrot from Malino Highland, Sub-district of Tinggimoncong, District of Gowa, South Sulawesi. Morphological identification was done based on character of the female perineal pattern. Molecular identification was based on amplification of r-DNA by polymerase chain reaction technique using species specific primers (Fjav/Rjav for M. javanica, Far/Rar for M. arenaria, and Finc/Rinc for M. incognita) and multiplex primer (JMV1/JMVhapla/JMV2 for M. hapla, M. chitwoodi, and M. fallax).Two of Meloidogyne species, i.e. M. incognita and M. arenaria were detected associated with the incidence of carrot branched tuber. The specific primers amplified two DNA bands, i.e. ± 999 bp of M. incognita and ± 420 bp of M. arenaria, while multiplex primer was failed to amplify DNA bands. Nucleotide sequence analysis showed M. incognita isolate of Malino was closely related to M. incognita isolate from Bangka-Indonesia, China (isolate JS2), and Malaysia (isolates JIK4, FIK4, JIT19, and FIT19) with homology of 99.2–100.0%. The nucleotide sequences of M. arenaria from Malino was submitted to GenBank with accession number KP234264, which was the first nucleotide sequence data in GenBank.

Co-Authors . Supramana Abdul Munif ALI NURMANSYAH Aprilyani Aprilyani Arif Kurniawan Asniwita Asniwita Erniawati Diningsih Giyanto Giyanto Halimah Halimah Hishar Mirsam I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I G. R. M. TEMAJA Kikin H Mutaqin Laras Anjarsari M. Tinny Lestariningsih Mochamad Yadi Nurjayadi Muhamad Taher Muhammad Rizal Ni Nengah Putri Adnyani Rita Kurnia Apindiati Rizki Haerunisa Sari Nurulita SEDYO HARTONO Slamet Susanto SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Suswi Nalis Teguh Pratama Tomohide Natsuaki TRI ASMIRA DAMAYANTI Zalzilatul Hikmia Hikmia

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