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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan
Ekowati Chasanah
Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides Dewi, Ariyanti Suhita; Hadi, Tri Aryono; Januar, Hedi Indra; Pratitis, Asri; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.4

Abstract

This experiment was conducted to study the effects of anthropogenic stressor on the spatialvariability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings wereconducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 kmoff Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquidchromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extractswas determined against T47D (breast) cancer cell lines. Results showed that the spatialproduction of aaptamine and isoaaptamine did not correlate with the quality of their surroundinghabitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts.
MOLECULAR IDENTIFICATION OF SPONGES OBTAINED FROM SERIBU ISLANDS NATIONAL PARK AND THEIR ASSOCIATED BACTERIA Patantis, Gintung; Rahmadara, Gemilang; Elfidasari, Dewi; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): May 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i1.80

Abstract

Sponges are simple multicellular animals that produced many pharmaceutical secondary metabolites. Some sponge-associated bacteria are proven to produce the same metabolites as their host, giving an opportunity to mass produce the potential metabolites. The aim of this research was to analyze the diversity of sponge-associated bacteria and to identify  the host sponge. Samples were collected from Seribu Islands National Park. Partial identification of sponges were conducted by molecular technique with the mitochondrial cytochrome oxidase subunit 1 (CO1) as the target area. The diversity of sponge-associated bacteria was determined by Terminal Restriction Fragment Length Polymorphism (T-RFLP) method. Result showed that sponges PS-17-12 has similarity with Petrosia sp., while PS-26-12 and PS-38-12 has similarity with Xestospongia muta. From the 3 sponge samples, 85 species of bacteria was obtained which can be classified into 9 phylums and 1 uncultured bacteria/environment sample. Some of  the sponge-associated bacteria identified were known as a potential producer of metabolites with antibiotic activity.
IDENTIFICATION AND CULTIVATION OF MFW 23-08 ISOLATED FROM MARINE SPONGES FOR BIOACTIVE COMPOUND PRODUCTION Chasanah, Ekowati; Pratitis, Asri; Mangunwardoyo, Wibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 2 (2012): August 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i2.16

Abstract

Production of marine bioactive compound for commercial usage has been hampered due tothe problem of raw material supply. To overcome this, marine microbes especially those associatedwith the bioactive-compound producer biota, has been explored as bioactive sources, with severaladvantages such as shorter production time, cheaper production cost and avoiding over exploitationof marine biota sources. Previous research showed that fungi MFW 23-08 was one of the potentialisolates from Wakatobi sponges which produced bioactive compounds that was active againstbreast cancer cell line and as antioxidant. This study was intended to identify MFW 23-08 andoptimize the production of its bioactive compound through optimization of MFW 23-08 culture.Culture optimization was conducted using 3 liquid media, i.e. malt extract broth (MEB), glucosepeptone yeast (GPY), and minimal fungal media (MFM), and cultivation periods, i.e. 2, 4, 6, 8, and10 wk. Results revealed that MFW 23-08 crude extract of 2 wk-MFM cultivation, at the concentrationof 30 μg/ml, was able to retard 87% breast cancer (T47D) cell growth. While at concentration of100 μg/ml, the 6 wk. MEB cultivated extract was able to hamper free radicals (56%). However, thecrude extract from MFM media cultivation, in the concentration of 50 and 100 μg/ml was not able toinhibit Escherichia coli and Staphylococcus aureus growth. Based on molecular identificationusing ITS1-ITS4 primers, MFW 23-08 isolate was 99% similar to  Penicillium citrinum, P.griseofolvum and Penicillium  sp.
IDENTIFICATION AND PARTIAL CHARACTERIZATION OF CRUDE EXTRACELLULAR ENZYMES FROM BACTERIA ISOLATED FROM SHRIMP WASTE PROCESSING Chasanah, Ekowati; Ali, Mahrus; Ilmi, Miftahul
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 1 (2012): May 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i1.11

Abstract

Attention on chitin degrading enzymes has been growing since their ability to reduce the waste of shrimp/other crustaceans processing industries and converting them into value added products such as biologically active chitin and chitosan oligomer. Previous experiment found that KLU 11.16 isolate was one of the potential bacteria isolated from shrimp waste producing chitinolytic enzymes including chitosanases. A study on the identification of KLU 11.16 extracellularcrude enzyme was carried out by cultivating the bacteria on chitin medium. Due to the wide application of chitosanase, the characterization of the crude chitosanase was carried out after an identification of the enzymes secreted. Based on assessment using zymogram technique, this bacteria secreted a mixed extracellular chitinolytic enzyme and other hydrolytic enzyme. The crudechitinolytic enzyme degraded 85% deacetylated (DA) better than 100% DA chitosan, and slightly degraded glycol chitin, indicating that KLU 11.16 secreted chitosanases and chitinases enzyme. In addition to the chitinolytic enzyme, the bacteria also secreted protein and carbohydrate degrading enzymes when running at SDS-PAGE enriched with casein, soluble starch and CMC substrates.Crude chitosanases enzyme was performed well at pH 6 and temperature of 300C, and the activity can be increased by addition of 1 mM Fe 2+ in form of chloride salt. Addition of detergent, i.e1mM of Triton X-100 and SDS slightly decreased the activity. Future application of the crude chitosanase from KLU 11.16 was on producing chitosan derivative such as chitosan oligomer using substrateof 85% DA chitosan, which is more digestable by other enzymes secreted by KLU 11.16
SEA CUCUMBER AS ANTICANCER AGENTS AND ITS DEVELOPMENT FOR FUNCTIONAL FOOD PRODUCTS Ridhowati, Sherly; Zakaria, Fransiska Rungkat; Syah, Dahrul; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 2 (2014): August 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i2.96

Abstract

Indonesia is popularly known as sea cucumber (teripang) exporter in the form of dried teripang. Commonly known as beche-de-mer or gamat, sea cucumber has long been used as medicine and food by Asian and Middle East people. Recent study  has shown that sea cucumbers contain active compounds that show potential health benefits and other biological properties such as antibacterial and antifungal products, anticoagulants, antihypertensives, immuno modulation, inhibitor of osteoclastogenesis. It was reported that sea cucumber posses aphrodisiacs, potentially improve immunity, anticancer and anticoagulation. Sea cucumber is also rich in collagen as a component of connective  tissue which can further be converted into smaller molecule and act as bioactive substances. This  review presents  the potential of sea cucumber as a functional food especially to prevent cancer and  strategy to develop sea cucumber-based functional food  by enzymatic hydrolysis and in vivo study.
PROTEIN DARI BIOTA LAUT DAN POTENSINYA DALAM INDUSTRI YANG MENGGUNAKAN TEKNOLOGI NANO-SILIKA Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 2, No 2 (2007): December 2007
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v2i2.137

Abstract

Peran protein dalam proses pembentukan biosilika yang terjadi di dalam tubuh beberapa biota laut telah menginspirasi peneliti untuk mempelajari dan meniru teknologi tersebut untuk aplikasi industri. Dengan proses biologi biasa, protein dari spikula spons dan dinding sel diatom dapat berfungsi menjadi cetakan dan mendirect proses biosilika berskala nano. Isolasi dan identifikasi protein tersebut, yang dilanjutkan dengan uji coba proses biosilika secara in vitro, memperlihatkan potensi protein tersebut sebagai biokatalis/agen biologi dalam sintesis silika. Sintesis silika secara kimia, seperti dalam produksi bahan resin, katalis, dll, selama ini dikenal merupakan proses yang tidak ramah lingkungan dan boros energi (diperlukan suhu, pH dan tekanan tinggi). Karena itu, eksplorasi dan riset lebih lanjut mengenai protein ini dirasa sangat penting mengingat bahan dasar silika, termasuk didalamnya teknologi nano, telah dipergunakan dalam berbagai bidang industri termasuk diantaranya industri pangan, elektronika, otomotif, dan lain-lain. Perkembangan riset tentang protein ini, baik yang telah dilakukan oleh peneliti luar negeri maupun yang dilakukan di Indonesia, serta aplikasi teknologi nano-silika akan disampaikan pada makalah ini.
Logic Structure Determination (LSD) as a Computer Assisted Structure Elucidation (CASE) for Molecular Structure Determination of Cytotoxic Cembranoids from Soft Coral Januar, Hedi Indra; Zamani, Neviaty Putri; Soedharma, Dedi; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.177

Abstract

Indonesian tropical soft corals are valuable resources that produce pharmacological cytotoxic cembranoids. However,  the manual structure determination in these compounds requires adequate knowledge of organic chemistry. This study presents the application of Logic Structure Determination (LSD) as a freeware Computer Assisted Structure Elucidation (CASE) for automatic molecular structure determination of cembranoid compounds from soft corals species. 12 Nuclear Magnetic Resonance (NMR) datasets of cytotoxic cembranoids were used to evaluate the accuracy of LSD in generating the possible structures. The results of this study shows that LSD generated numerous possible molecular structures as the data input files were only derived from 2D-NMR HMQC (Heteronuclear Multiple-Quantum Correlation), COSY (Homonuclear Correlation Spectroscopy), and HMBC (Heteronuclear Multiple Bond Correlation). The accuracy was significantly enhanced (only 2-4 possible cembranoid structures from each NMR dataset) with the addition of H2BC (Heteronuclear 2 Bond Correlation) experimental data. This may indicate that VLRC (Very Long-Range Correlations) significantly affects LSD capability. Furthermore, LSD with direct 2 bond NMR experimental data is a reliable CASE technique for cembranoid compounds identification. In general, this freeware-CASE has the potential to be applied  on other types of small molecule compounds and may serves as a solution for elucidation bottleneck step in studies on Indonesian natural products.
Nutritional and Albumin Content of Swamp Fishes from Merauke, Papua, Indonesia Susilowati, Rini; Sugiyono, Sugiyono; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.268

Abstract

 Study on chemical content of swamp fishes from Merauke has been conducted to obtain nutritional status  of these fishes. Sampling was conducted  twice (August and November 2015) in Merauke. There were six dominant fish species, namely striped snakehead (Channa striata), nile tilapia (Oreochromis niloticus), tade gray mullet (Liza tade), philippine catfish (Clarias batrachus), barramundi (Lates calcarifer), and climbing perch (Anabas testudineus). Result on proximate analysis showed that the fishes had moisture content of 75.73±0.25%-81.45±0.04%, ash 0.94±0.01%-1.26±0.21%, protein 17.11±0.09%-18.92±0.05%, and lipid 0.59±0.19%-3.80±0.63%. The dominant essential amino acid of 6 swamp fishes from Merauke was lysine followed by leucine. Meanwhile the dominant non-essential amino acid was glutamic acid (32.26+0.61 mg/g), followed by aspartic acid. Calcium (Ca) was the dominant macro mineral for all fishes studied, being the highest was in nile tilapia (328.76±8.14 mg/100 g). Micro mineral was dominated by the presence of Selenium (Se) which the highest was in nile tilapia (0.084±0.005 mg/100g). These fishes were also rich in albumin, being the highest was stripe snakehead (138.59±1.68 mg/g). The average of total fatty acid showed that the fishes had saturated fatty acid (SFA) content of 46.30±0.27%, monosaturated fatty acid (MUFA) 9.20±1.6%  and polyunsaturate fatty acid (PUFA) 34.49±0.44% of total fatty acid. From the result, it can be concluded that fish harvested from swamp of Merauke and surrounding area had a good nutritional value.
Penapisan Senyawa Hemagglutinin dari Makroalga Asal Pantai Binuangeun, Banten, Indonesia Fajarningsih, Nurrahmi Dewi; Praseptiangga, Danar; Chasanah, Ekowati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): Juni 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i1.241

Abstract

Sebagai bagian dari penelitian penapisan lektin dari makroalga Indonesia, 17 esktrak protein makroalga yang dikoleksi dari Pantai Binuangeun, Banten telah diuji aktivitas hemagglutinasinya terhadap eritrosit kelinci dan eritrosit manusia golongan A, B, O, masing-masing dengan perlakuan enzim dan native. Ekstraksi dilakukan dengan menggunakan 2 jenis buffer, yaitu Phosphate Buffer Saline (PBS) dan Tris Buffer Saline (TBS) pH 7. Hasil penelitian menunjukkan bahwa pada beberapa sampel, ekstrak yang dihasilkan kedua buffer, menunjukkan aktivitas hemagglutinasi yang berbeda walaupun kadar total protein ekstrak makroalga yang diekstraksi dengan PBS dan TBS tidak berbeda. Sebagian besar ekstrak makroalga yang diuji mampu mengagglutinasi setidaknya satu jenis sel eritrosit yang digunakan. Secara umum, kelompok makroalga hijau memperlihatkan aktivitas hemagglutinasi yang lebih rendah dibandingkan kelompok makroalga merah dan coklat. Meskipun ekstrak Padina australis (makroalga coklat) memberikan hasil hemagglutinasi eritrosit kelinci negatif, namun ekstrak tersebut positif menghemagglutinasi eritrosit golongan darah B dan O. Di antara 8 makroalga hijau yang diuji, hanya dua sampel yang menunjukkan aktivitas hemagglutinasi, yaitu Chaetomorpha crassa dan Halimeda macroloba. Keempat ekstrak makroalga merah yang diuji menunjukkan aktivitas hemagglutinasi yang kuat terhadap eritrosit kelinci. Ekstrak makroalga merah Gracilaria lichenoides dan Gelidiella acerosa aktif terhadap semua jenis eritrosit uji. Sementara itu, hanya ekstrak Laurencia tronoi yang menunjukkan aktivitas hemagglutinasi terhadap eritrosit golongan darah A.
Produksi Senyawa Bioaktif dari Aspergillus ustus MFW 26-08 yang Berasosiasi dengan Spons Laut dalam Berbagai Media Pratitis, Asri; Patantis, Gintung; Mangunwardoyo, Wibowo; Chasanah, Ekowati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 2 (2010): Desember 2010
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v5i2.411

Abstract

Penggunaan mikroba sebagai sumber senyawa bioaktif memiliki beberapa kelebihan di antaranya mempersingkat waktu produksi dan menghindari pemanfaatan sumberdaya laut secara berlebih. Penelitian terdahulu menghasilkan beberapa isolat mikroba yang berpotensi sebagai penghasil senyawa bioaktif, di antaranya adalah isolat kapang MFW 26-08. Penelitian ini bertujuan untuk melakukan optimasi pertumbuhan isolat kapang MFW 26-08 dan produksi senyawa bioaktif yang disekresikan oleh kapang tersebut. Optimasi dilakukan dengan menggunakan 3 jenis media: Malt Extract Broth (MEB), Glucose Peptone Yeast (GPY), dan Minimal Fungal Media(MFM); serta waktu kultivasi 2, 4, 6, 8, dan 10 minggu. Hasil riset menunjukkan bahwa ekstrak kasar kapang MFW 26-08 hasil kultivasi 2 minggu dalam medium MFM, pada konsentrasi 30 µg/mL, mampu menghambat 89% pertumbuhan sel kanker payudara T47D. Sedangkan pada konsentrasi 100 µg/mL ekstrak kapang yang dikultivasi dalam medium MEB selama 6 minggu, mampu menghambat pembentukan radikal bebas sampai 56%. Hasil identifikasi berdasarkan sifat-sifat morfologi dan molekuler menggunakan 18S rRNA, ITS1, dan ITS4 menunjukkan bahwa isolat MFW 26-08 memiliki kemiripan dengan Aspergillus ustussebesar 99%
Co-Authors ARIYANTI SUHITA DEWI DAHRUL SYAH Danar Praseptiangga DEDI SOEDHARMA Dewi Elfidasari ENDAR MARRASKURANTO FRANSISKA RUNGKAT ZAKARIA Gintung Patantis HEDI INDRA JANUAR Mahrus Ali Miftahul Ilmi, Miftahul Muhammad Nursid Neviaty P Zamani Nita Noriko Noviendri, Dedi Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Pratitis, Asri Rahmadara, Gemilang Ridhowati, Sherly Rini Susilowati, Rini Sugiyono Sugiyono Tri Aryono Hadi WIBOWO MANGUNWARDOYO Yusro Nuri Fawzya