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Biopotensi Tumbuhan Mangrove untuk Pencegahan Penyakit Vibrosis pada Udang Windu ., Melki; Soedharma, Dedi; Effendi, Hefni; Mustopa, A. Zaenal
Maspari Journal : Marine Science Research Vol 2, No 1 (2011): Edisi Januari
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.912 KB) | DOI: 10.36706/maspari.v2i1.1146

Abstract

Ekstrak tiga jenis tumbuhan mangrove (daun, buah, batang dan akar) meliputi tumbuhan mangrove Ceriops tagal, Rhizophora apiculata, dan Sonneratia alba yang diambil dari Sadai, Bangka Selatan, Bangka Belitung dimana telah diekstraksi dengan pelarut methanol, ethyl acetate dan n-hexane serta telah diuji sebagai antibakteri pada bakteri Vibrio harveyi yaitu patogen udang windu dan uji toksisitas BSLT. Hasil uji antibakteri dari semua bagian tumbuhan mangrove didapatkan bahwa daun dan batang S. alba dengan pelarut metanol lebih potensi yaitu dengan zona hambat yang terbentuk 24±3,78 mm dan 23±3,78 mm. Hasil uji BSLT didapatkan untuk daun S. alba dengan pelarut metanol bersifat toksik sedangkan batang S. alba dengan pelarut metanol bersifat tidak toksik terhadap artemia. Selanjutnya bagian tumbuhan mangrove dianalisa komponen kimianya dengan menggunakan kromatografi kolom, kromatografi lapis tipis dan kromatografi cair kinerja tinggi dengan detektor PDA. Hasil identifikasi dengan menggunakan kromatografi kolom dengan eluen chloroform: methanol (9:1 sampai 1:9) memberikan hasil pemisahan terbaik, identifikasi dengan menggunakan kromatografi lapis tipis dengan eluen chloroform: methanol (9:1 sampai 1:9) memberikan hasil pemisahan terbaik dan identifikasi dengan kromatografi cair kinerja tinggi pada panjang gelombang 200-400 nm menghasilkan bahwa ekstrak tumbuhan mangrove yang berperan sebagai antibakteri dan untuk selanjutnya ekstrak tumbuhan mangrove ini dapat dikembangkan sebagai antibakteri dan biofarmatika.   Kata kunci: ekstrak mangrove, antibakteri, BSLT, kromatografi kolom , kromatografi lapis tipis, dan kromatografi cair kinerja tinggi ABSTRACT Crude extracts of three mangrove species (leaf, fruit, bark and root), i.e. Ceriops tagal, Rhizophora apiculata, and Sonneratia alba collected from Sadai, South Bangka, Bangka Belitung was extracted  in methanol, ethyl acetate, n-hexane and tested for different range of biological activities including antimicrobial activity of shrimp Vibrio harveyi pathogen and brine shrimp cytotoxic. The overall bioactivity profile showed that, leaf and bark of S. alba methanolic (24±3,78 mm and 23±3,78 mm) exhibited more biopotency. Result by using Brine Shrimp Lethality Test showed that leaf of S. alba methanolic was toxic and bark of S. alba methanolic was not toxic to Artemia. The highly active mangrove was evaluated further to analyze the chemical compounds using column chromatography, thin layer chromatography and high performance liquid chromatography with detector photodiode array (PDA). The identification with column chromatography using chloroform: methanol (9:1 to 1:9) gave the best separation, identification results using thin layer chromatography using chloroform: methanol (9:1 to 1:9) gave the best separation and chromatographic identification results high performance liquid at a wavelength of 200-400 nm showed mangrove extracts might have functional role in bioactivity and can be used for the development of antibacterial and biopharmaceuticals.   Keywords: Mangrove extract, antibacterial activity, brine shrimp cytotoxicity, column    chromatography, TLC, and HPLC
Seksualitas dan Perkembangan Gamet Sponge Laut Aaptos aaptos Schmidt Haris, Abdul; Soedharma, Dedi; Zamani, Neviaty P.; Pariwono, John I.; Rachmaniar, Rachmaniar
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.347 KB) | DOI: 10.31258/jnat.14.3.205-211

Abstract

This research was aimed to know the characteristics of gamet development of marine sponge Aaptos aaptos living in tropical waters of Barrang Lompo Island, Spermonde Archipelago, South Sulawesi. In order to know gamet development, it was conducted three periods of sample collection at each moon phase. After sample collection, the specimen were put into tissue cassette and then were removed to fixative solution of FAACC (for 100 mL = 10 mL formaldehyde solution of 37–40%: 5 mL glacial acetic acid: 1.3 g calcium chloride dihydrate: 85 mL destilate water) for +48 hours, and then were removed to 70% alcohol for temporary storage before doing histological preparation following standard procedure. Sexuality of marine sponge Aaptos aaptos living in Barrang Lompo Island is gonochoric. Spermatocyt developed in spermatic cyst, while oocyt developed in the mesohyl. Stage of male gamet development was divided into four phases i.e. spermatocyt I phase, spermatocyt II phase, spermatocyt III phase, and spermatocyt IV (spermatid) phase, similarly, female gamet develop- ment was divided into four phases i.e. oocyt I phase, oocyt II phase, oocyt III, oocyt IV phase. Each phase of gamet development had specific characterstics different from among each others.
Teknologi Fragmentasi Buatan Karang (Caulastrea furcata dan Cynarina lacrimalis) dalam Upaya Percepatan Pertumbuhan pada Kondisi Terkontrol Zulfikar, Zulfikar; Soedharma, Dedi
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.137 KB) | DOI: 10.31258/jnat.10.2.76-82

Abstract

The objective of research were analyze water quality condition of water circulation system at laboratory and tomeasured growth survival rate of Caulastrea furcata and Cynarina lacrimalis which was fragmented at laboratory.Fragmentation treatment of Caulastrea furcata become 1, 2, 3, and 4 polyp that was rearing on circulation systemdid not give significant impact on height and length growth after 160 days rearing and fragmentation of Cynarinalacrimalis on circulation system give significant impact. Mean of growth length of Caulastrea furcata on treatment1, 2, 3 and 4 polyp in every month after 160 days was 1.64 mm, 1.55 mm, 1.42 mm , and1.08 mm whereas growthbroad was 0.71 mm, 0.82 mm, 0.51 mm, 0.62 mm, and mean of growth length Cynarina lacrimalis for the sametreatment in every month was 1.47 mm, 0.90 mm, 0.62 mm, 0.61 mm whereas growth broad was 1.57 mm, 1.16mm, 0.93 mm, 0.89 mm. Fragmentation treatment of Caulastrea furcata become 1 polyp was best length if compareother treatment and Cynarina lacrimalis was treatment became 2 devide.
Analisis Preferensi Visual Lanskap Pesisir Daerah Istimewa Yogyakarta untuk Pengembangan Pariwisata Pesisir Menuju pada Pengelolaan Wilayah Pesisir Berkelanjutan Khakhim, Nurul; Soedharma, Dedi; Mardiastuti, Ani; Siregar, Vincentius P.; Boer, Mennofatria
Forum Geografi Vol 22, No 1 (2008): July 2008
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/forgeo.v22i1.4925

Abstract

The aim of this research is to analyze of DIY coastal landscape with visual preference analysis for suistanble coastal tourism development and management. The unit of analysis that used is coastal typology. The guideline in deciding the classification of coastal typology is using the Response-Process System with relief/slope, main constructing material, genesis process and dominate process happened in the meantime such as tide, wave and river flow. This response-process system divide the coastal typology into seven classes including coastal typology of land erosion coast, sub aerial deposition coast, volcanic coast, structurally shaped coast, wave erosion coast, marine deposition coast and coast built by organism. The method of SBE (Scenic Beauty Estimation) is used for visual preference analysis, and the method used to compose the policy of costal tourism development is SWOT method. Result shows that all seven coastal typology are found in the coastal area. Land erosion coast and coast built by organism dominate in Gunungkidul coastal area and then in Bantul and Kulon Progo coastal area are dominated by marine deposition coast and sub aerial deposition coast. Volcanic coast, structurally shaped coast, wave erosion coast can only be found in a small area of Gunungkidul coast. Each of this coastal typology has a special land characteristic which can be used to develop its potential. Coast built by organism is very suitable for tourism activity proved by the high score of SBE from the respondents. Recommendation for developing coastal area in area of interest is by developing the coastal natural resources suitable to its physical typology, because this will make the management of coastal area for continuous development easier. Recommendations for coastal management in Gunungkidul including mapping and classification of protected karst area and mineable karst area to secure the run of coastal area management, for coastal management in Bantul using Managed realignment which plans for retreat and adopts engineering solutions that recognise natural processes of adjustment, and identifying a new line of defence where to construct new defences and move seaword model by constructing new defenses seaward the original ones. Last, for Kulon Progo coastal area using hold the line model whereby seawalls are constructed around the coastlines.
Periode Pemijahan Spons Aaptos aaptos (Porifera: Demospongia) di Perairan Pulau Pari, Kepulauan Seribu, DKI Jakarta Kawaroe, Mujizat; Soedharma, Dedi; Siregar, Rahmadsyah Deny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.583 KB) | DOI: 10.24002/biota.v13i2.2673

Abstract

The purpose of this research was to study spawning period of sponge Aaptos aaptosat Pari Island, Thousand Islands, DKI Jakarta. The result showed that spawningtime occurred at 17.00-18.00, and within that time the sponge closed their osculumto throw out the zygote leaving the body faster. The duration of spawning rangefrom 4 minutes to 41 minutes. Based on the lunar periode, spawning for spongeAaptos aaptos started from early new moon till few days after full moon. Spawningmostly took place during spring tides. Based on the PCA analysis, it was found thatwater temperature and pressure had strong correlation with spawning time.
Pengaruh Suhu dan Salinitas Terhadap Respon Fisiologi Larva Tiram Mutiara Pinctada maxima (Jameson) Winanto, Tjahjo; Soedharma, Dedi; Affandi, Ridwan; Sanusi, Harpasis S.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.755 KB) | DOI: 10.14203/jbi.v6i1.3171

Abstract

ABSTRACTPENDAHULUANPinctada maxima adalah spesiesakuakultur yang mempunyai nilaiekonomi tinggi (Taylor et al. 1997). Dipasaran internasional, mutiara yangdiproduksi sering kali disebut dengannama “South Sea Pearl”. Indonesiatermasuk salah satu negara penghasilmutiara (South Sea Pearl) yang cukupdiskenal di pasaran dunia, sebagian besarproduksi South Sea Pearl yang dipasarkanberasal dari hasil budidaya (Anna2006). Produksi mutiara berbasisbudidaya merupakan aktivitas usaha yangmenguntungkan. Perkembangan usahabudidaya mutiara saat ini sudah mengarahpada kegiatan industri yang terintegrasi(Fassler 1995).The Effect of Temperature and Salinity to The Physiological Respons on The Larvae ofPinctada maxima (Jameson). Energy budget is one of the most sensitive tools available forindividual assessing environmental changes like temperature and salinity, and also prerequisitefor individual growth and survival. The aim of this study is to obtained information on energybudget on routine metabolism, in different levels of temperature and salinity, and to know thelevels of optimum temperature and salinity. The research was used randomized block design,with three replications. The result showed that optimal temperature and salinity on P. maximalarvae was 28 oC and 32 – 34 ‰ (BE and BF). Energy budget to routine metabolism increasedwas attributed to increased temperature and salinity due to the optimal, than would be decreasedwhen temperature and salinity increased. The highest of energy budged for routine metabolismat treatment BF. Stage I: energy budged between 6.73 – 7.35 C g wet weight-1 hour-1 (28.18 –30.74 J g wet weight-1 hour-1); Stages II: 5.85 – 5.95 C g wet weight-1 hour-1 (24.48 – 24.90 J g wetweight-1 hour-1); Stages III: 4.73 – 4.80 C g wet weight-1 hour-1 (15.07– 19.58 J g wet weight-1hour-1). The highest survival rate of larvae was by treatment BF, but has not higher significant(P e” 0.05) with BE, stage I: survival rate between 87.75 – 87.92 %; Stage II: 81.91 – 82.39 % andstage III: 76.72 – 77.26 %. The best of relative growth length of larvae by treatment BF and notsignificant (P e” 0.05) with BE, at stage I: 29.78 x 17.93 ?m – 30.57 x 18.43 ?m (AP x DV); stageII: 57.62 x 46.73 ?m – 58.13 x 47.33 ?m and stage III: 80.32 x 69.29 ?m – 80.88 x 69.62 ?m. Thequickest time of plantigrade stages have found by treatment BF (day 19.50) and hasn’tsignificant (P > 0.05) with BE (day 20.85).Keywords: Pinctada maxima, larvae, response; physiology, metabolism.Kata kunci: Pinctada maxima, larvae, respon, fisiology, metabolisme.
Produktitvitas Diatom Perifitik yang Ditumbuhkan pada tipe Subtrat Berbeda Sebagai Alternatif Penyediaan Pakan Alami Udang Pratiwi, Niken T.M.; Adiwilaga, Enan M.; Widigdo, Bambang; Soedharma, Dedi
JURNAL BIOLOGI INDONESIA Vol 4, No 3 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5695.068 KB) | DOI: 10.14203/jbi.v4i3.3262

Abstract

ABSTRACTThe Productivity of Periphytic Diatom Grown on Different Types of Substrates asan Alternative on Providing Natural Feed for shrimp. Providing natural feed is anattempt to support fisheries culture in a polite way for the sustainability of environment.Periphytic community is living aquatic resources that have a potential value for thispurpose. The major component of saline water periphytic community is diatom.The study was emphasized on diatom presentation grown on two types of artificialsubstrates: biocrete (sand, palm fiber, cement) and zeocrete (zeolite, palm fiber, cement).The reseach was conducted in laboratory with an experimen design follows split splitplot in times. The aim of the research is to study the ecological factor and availablenutrients to support the growth of periphitic diatoms (Amphora, Cyclotella, Melosira,Navicula, Phaeodactylum, and Thallassiosira) on two different substrates. Theproductivity of diatom, and the effect of shrimp larvae on the diatom productivity werealso analyzed. Two fertilized materials (biocrete and zeocrete) with two main treatmentsand three levels of treatments each, and unfertilized materials were used as artificialsubstrates for periphitic diatoms. The results show that all treatment could give sufficientbiological available nutrient for the diatoms. The highest diatom productivity was achievedby the population on third level ratio of fertilized biocrete and zeocrete (added by fertilizedwith N:P ratio of 30:l). Diatom productivity follows the shrimp larvae grazing. Theshrimp larvae could grow well on the media with diatom that were grown on fertilizedbiocrete.Key words: periphytic diatoms, natural feed, biocrete, zeocrete, productivity
KETERKAITAN STRUKTUR KOMUNITAS MEIOFAUNA DENGAN KERAPATAN LAMUN DAN PARAMETER LINGKUNGAN DI SELAT DOMPAK, KEPULAUAN RIAU Zulkifli, Zulkifli; Soedharma, Dedi; Wardiatno, Yusli; Sanusi, Harpasis S.
Jurnal Perikanan dan Kelautan Vol 12, No 01 (2007)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.12.01.15-22

Abstract

A study on the relationship between meiofauna community structure with seagrassdensity and environmental parameters has been carried out in the Dompak Straitwaters, Riau Archipelago. This study were investigated on four different seasonsthroughout a year, from May 2005 to April 2006. Three sampling site, i.e.., Al, A2and A3 were chosen to represent research sites. The sediment samples were tekenby PVC cores, in which six layers were separated (i.e. 0-5 cm, 5-10 cm, 10-15 cm,15-20 cm, 20-25 cm and 25-30 cm). The environmental parameters, such ascurrent velocity, the sediment composition, total organic carbon (TOO, totalorganic matter (TOM), pH and redox potential (Eh), were also observed. The dataon meiofauna, seagrass density and environmental parameters were analyzedstatistically by a regression analyisis. This analysis was run using a SPSS softwareversion 11.5 for Windows. The meiofauna was dominated by Nematodes,Copepods, Polychaetes, Foraniiniferans and Turbellarians. The results areshowing that the meiofaunal community structure would depend on severalconditions such as seagrass density, environmental factors and hydrologicalcharacteristics. The regression results are showing that the meiofaunal communitystructure patterns was significantly correlated with seagrass density and to theenvironmental parameters. Vertically, the meiofaunal community structurepatterns seems to be more affected by TOC and TOM. While the horizontally, itseems to he more affected by current velocity, TOM and pH.
DETEKSI LOGAM BERAT PADA PERAIRAN, SEDIMEN DAN SIRIP IKAN BADUKANG {Anus caelatus HAN A. maculatus) DIMUARA SUNGAI KAHAYAN DAN SUNGAI KATINGAN, KALIMANTAN TENGAH Harteman, Edison; Soedharma, Dedi; Winarto, Adi; Sanusi, Harpasis S
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.783

Abstract

The study sites were located in the river mouths of Kahayan and Katingan Rivers of Central Kalimantan. The purpose of this study was to evaluate concentration of heavy metals on the water, sediment, Arius (catfish) fin through concentration and bioconcentration factor (BCF); data were analysed by regression and correlation analysis. Collected samples were water and fish.Water sample and fish bone were analysed by Atomic Absorption Spectrophotometer (AAS). The results indicated that the concentrations of Pb>Cd>Hg were occurred in the water and hard fins, which the bioconcentrations of Hg>Cd>Pb were in the hard fins, while the bioconcentration factors (BCFs) of Pb>Hg>Cd were in the sediment.Moreover, it seems that heavy metals in the waters absorpted and accumulated by sediment and hard fins increased along with heavy metal concentration in the water, whereas heavy metals in the sediment absorpted and accumulated by hard fins increased along with heavy metal concentration .in the sediment.
KUALITAS AIR SUNGAI CISADANE, JAWA BARAT - BANTEN Siahaan, Ratna; Indrawan, Andry; Soedharma, Dedi; Prasetyo, Lilik B
JURNAL ILMIAH SAINS Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.11.2.2011.218

Abstract

KUALITAS AIR SUNGAI CISADANE, JAWA BARAT - BANTEN Ratna Siahaan1), Andry Indrawan2), Dedi Soedharma2), dan Lilik B.Prasetyo2) 1)Mahasiswa S3 Sekolah Pascasarjana IPB, Dosen Universitas Sam Ratulangi; e-mail:ratna245_siahaan@yahoo.com; 2)Dosen Sekolah  Pascasarjana IPB, Bogor ABSTRAK Sungai Cisadane memiliki fungsi dan nilai untuk kesejahteraan manusia dan hidupan liar yang hidup di dalam sungai. Kegiatan manusia yang memanfaatkan air sungai dan membuang sampah/limbah ke Sungai Cisadane dapat menurunkan kualitas air Sungai Cisadane. Penelitian ini bertujuan untuk menganalisis kualitas air S.Cisadane berdasarkan faktor fisika dan kimia air sungai. Penelitian dilakukan di sembilan (9) titik di sepanjang Sungai Cisadane dari hulu hingga hilir pada Agustus-November 2011. Hasil menunjukkan jika air Sungai Cisadane telah tercemar. Secara umum, kualitas air Sungai Cisadane di bagian hulu dan tengah (Stasiun 1-6) masih dapat dipergunakan sebagai air untuk peruntukan Kelas 2 (PP.No.82/2001) dengan kualitas air tercemar ringan. Namun, air Sungai Cisadane di bagian hilir (Stasiun 7-8) hanya untuk peruntukan Kelas 3 dan 4 dan dikategorikan tercemar parah. Kata kunci: kualitas air, sungai Cisadane   WATER QUALITY OF CISADANE RIVER, WEST JAVA-BANTEN ABSTRACT Cisadane River has functions and values for human welfare and wildlife. Human activities in using river water and dumping waste to the river could decrease water quality of Cisadane River. The aim of this research was to analysis water quality of Cisadane River based on physical and chemical factor.  Water sampling was conducted along Cisadane River from up to downstream at nine (9) stations. Result showed that Cisadane River is polluted. Generally, water quality of Cisadane River in up and middle part (Station 1-6) could be used for purposes as in Class 2 (Gov.Reg/PP No.82/2001) and classified into moderately polluted. But, water quality of down Cisadane River (Station 7-9) only for Class 3 and 4 that categorized into heavily polluted. Keywords: Cisadane river, water quality