. Muttaqien
Fakultas Kedokteran Hewan Universitas Syiah Kuala, Jl. Tgk. H. Hasan Krueng Kale No. 4 Darussalam, Banda Aceh 23111, Indonesia.

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Perbandingan Aktivitas Antelmintik Albendazole dan Levamisole terhadap Ascaridia galli secara In Vitro Ummu Balqis; Muhammad Hambal; . Darmawi; Abdul Harris; . Rasmaidar; Farida Athaillah; . Muttaqien; . Azhar; . Ismail; Razali Daud
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.142 KB) | DOI: 10.29244/avi.4.2.97-102

Abstract

Penelitian ini meneliti aktivitas antelmintik albendazole dan levamisole terhadap hambatan motilitas, percepatan waktu paralisis, dan motilitas cacing Ascaridia galli dewasa secara in vitro. Sebanyak empat ekor cacing masing dibuat triplikat dalam NaCl 0,9% masing-masing dengan konsentrasi 15 mg/ml Albendazole, dan 0.6 mg/ml Levamisole. Motilitas cacing diamati pada interval 10, 20, 30, dan 40 jam. Paralisis dan kematian diamati pada tampilan tidak ada pergerakan badan pada bagian kepala dan ekor cacing. Hasil menunjukkan bahwa aktivitas albendazole dan levamisole terhadap mortalitas A. galli berturut-turut terjadi pada 40 dan 30 jam pasca inkubasi. Levamisole dapat menghambat motilitas A. galli pada jam ke 10 dan juga menyebabkan lebih awal paralisis pada 6,75 ± 0,50 jam pasca inkubasi. Kajian tersebut mengindikasikan bahwa aktivitas antelmintik levamisole lebih awal dibandingkan efek albendazole pada cacing A. galli.
Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii) Ummu Balqis; Darmawi Darmawi; Maryam Maryam; Muslina Muslina; Abdullah Hamzah; Razali Daud; Muhammad Hambal; Rinidar Rinidar; Abdul Harris; Muttaqien Muttaqien; Azhar Azhar; Eliawardani Eliawardani
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.142 KB) | DOI: 10.17969/agripet.v16i1.3022

Abstract

ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli.  (Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii) ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.
IDENTIFIKASI PARASIT PADA IKAN TONGKOL (Euthynnus affinis) DI TEMPAT PELELANGAN IKAN LHOKNGA ACEH BESAR (Identification of Parasites in Mackerel (Euthynnus affinis) at Fish Auction in Lhoknga Aceh Besar) Nurul Hidayati; Muttaqien Bakri; Rusli Rusli; Yudha Fahrimal; Muhammad Hambal; Razali Daud
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.344 KB) | DOI: 10.21157/j.med.vet..v10i1.4027

Abstract

The objective of this study was to identify parasites that infect mackerel fish (Euthynnus affinis) at fish auction in Lhoknga, Aceh Besar. Fifteen mackerel fish collected from fish auction in Lhoknga were examined at Parasitology Laboratory, Faculty of Veterinary Medicine Syiah Kuala University Banda Aceh to observe the presence of ectoparasites and endoparasites. The ectoparasite examination was conducted by observation of the external body part and then examined under microscope, while endoparasite examination conducted by observation of inner surface of digestive tract. The data showed that two types parasites infested the mackerel fish at fish auction in Lhoknga were Anisakis simplex and Neobenedia melleni. A total of 87% mackerel fish was infested with A. simplex and 7% was infested with N. melleni.
20. Ectoparasites Identification of Stingrays Fish (Dasyatis Sp.) At Peunayong Fish Market, Banda Aceh Muttaqien Muttaqien; Arini Ulfa Khaira; Winaruddin Winaruddin; Eliawardani Eliawardani; Muhammad Hambal; Al Azhar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.3547

Abstract

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages.The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks. The distribution ofHassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer
4. Haugh Unit Value, Yolk Index And Albumin Index Of Eggs In Farms, Distributors And Retails In Banda Aceh Andi Novita; Alingga Savira Edi Putri; Azhari Azhari; Rastina Rastina; Muttaqien Bakri; Amiruddin Amiruddin; Fadli A Gani; M Daud AK
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.22189

Abstract

This study aims to determine the quality of eggs from Farm, distributors and retail stores. The sample used is 66 eggs with details taken randomly at the farm is 9 eggs, distributors is 27 eggs and retail stores is 30 eggs. The observed egg quality is the value of Haugh unit, Yolk Index and Albumin Index. The data obtained were analyzed descriptively. The results showed that the average Haugh value of egg units in farms, distributors and retail stores were 91, 72 and 7. The Yolk Index values for eggs in farms, distributors and retail stores were 0.45, 0.35. and 0.36. Meanwhile, the albumin index of eggs in farms, distributors and retail stores was 0.074, 0.050 and 0.046. Egg quality in farms, distributors and retail stores is in the category (grade) AA (HU value 72) and A (HU value 60-72). Based on the results of this study, it can be concluded that the quality of eggs traded in Banda Aceh and its surroundings is very good (included in the AA and A grade categories).
Identification of Parasites in Snakehead Fish (Channa striata) in Meunasah Manyang Village Lhoknga Subdistrict Aceh Besar Adil Umara; Muttaqien Bakri; Muhammad Hambal
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.491 KB) | DOI: 10.21157/j.med.vet..v8i2.3324

Abstract

The study aimed at identifying the types of parasites that infect snakehead (C. striata) fish as well as to determine the prevalence of the parasite species. A total of 51 snakehead fish was captured using nets and traps then examined at Parasitology Laboratory of Veterinary Medicine Faculty Syiah Kuala University Banda Aceh. Ectoparasites inspection was done by observation of the outer body organs as well as by making the mucus smear examined under a microscope, while endoparasites inspection was done by observation on the surface of the digestive tract and heart, and also thin and thick boold smear. The study revealed that two types of parasites that infect snakehead fish in irrigation Cot Balee were Pallisentis nagpurensis and Trichodina spp. It also found larvae obtained from the intestinal villi scrapings that have not been identified. A total of 96% snakehead fish infected by P. nagpurensis, 6% infested with Trichodina spp., and 10% infested by unidentified larvae.Key words: parasite, snakehead fish (Channa striata), Palllisentis nagpurensis, Trichodina spp, prevalence
EFEK PENGGUNAAN KETAMIN-XILAZIN DAN PROPOFOL TERHADAP DENYUT JANTUNG DAN PERNAFASAN PADA ANJING JANTAN LOKAL (Canis familiaris) (Effect of Ketamine-Xylazine and Propofol on Heart Rate and Breathing Frequency of Local Male Dog (Canis familiaris)) Arman Sayuti; Ria Maulizar; Syafruddin Syafruddin; Erwin Erwin; Frengky Frengky; Muttaqien Muttaqien; Budianto Panjaitan; Zuraidawati Zuraidawati
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.951 KB) | DOI: 10.21157/j.med.vet..v10i1.4034

Abstract

The purpose of this study was to determine the effect of using ketamine-xylazin and propofol on heart rate and breathing in local male dogs (Canis familiaris). Six male dogs with average body weight ±2-3 kg were divided into 2 treatment groups, each group was subcutaneously injected with of atropine sulfate at dose of 0.04 mg/kg as premedication prior to anesthetic administration. The first group (K1) was intravenously injected with anesthetic combination of ketamine-xylazine at dose of 5.5 mg/kg and 1 mg/kg, respectively. The second group (K2) was given propofol at dose of 6.6 mg/kg intravenously. The heart rate and breathing were directly calculated using stethoscope and stopwatch with 10 minutes interval. Data were analyzed statistically using analysis of variance (Anova) with split-splot design and if there is a difference between the two groups followed by least significant difference test (LSD). The administration of ketamine-xylazin (K I) significantly (P0.05) increase the respiratory frequency compared to propofol administration (K2). The interaction between time and precise observation of K1 and K2 on 30 minute observation (W3) showed significant differences (P0.05). While on other time observations did not reveal any differences in the interaction between time and observation (P0.05). It can be concluded that there is no difference effects of given ketamine-xylazin and propofol against heart rate on local male dogs. But there was difference effect of administration ketamine-xylazin and propofol on respiratory frequency on local male dogs.
Identifikasi Keberadaan Nyamuk Aedes Spp Vektor Demam Berdarah Dengue (DBD) Di Gampong Pineung Kecamatan Syiah Kuala Banda Aceh Dina Izzatina; Farida Athaillah; Muhammad Hanafiah; Lian Varis Riandi; Eliawardani Eliawardani; Winaruddin Winaruddin; Muttaqien Muttaqien; M. Isa
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 1 (2022): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i1.8751

Abstract

ABSTRAKPenelitian ini bertujuan mengetahui keberadaan dan distribusi nyamuk Aedes  spp sebagai vektor  demam berdarah dengue (DBD) dengan menggunakan ovitrap di kawasan Gampong Pineung, Kecamatan Syiah Kuala  baik didalam ruangan (indoor) maupun diluar ruangan (outdoor). Penelitian ini dilakukan di lima dusun di Gampong Pineung kecamatan Syiah Kuala yaitu:  Tgk Chik Dipineung,  Bintara Pineung, Tgk Hasyim, Tgk Tengoh, dan T Muda Rayeuk. Hasil pengamatan terhadap total rata-rata telur nyamuk Aedes spp di kelima dusun  tidak memperlihatkan perberbedaan yang nyata (P0,05). Sedangkan jumlah total rata-rata telur nyamuk indoor dan outdoor antar dusun terdapat perbedaan yang nyata (P0,05). Tetapi  pada pengamatan terhadap Jumlah rata-rata larva Aedes agypti pada indoor berbeda nyata (P0,05) dibandingkan dengan larva Aedes albopictus. Begitu pula dengan jumlah rata-rata larva Aedes agypti pada outdoor berbeda nyata (P0,05) dibandingkan dengan larva Aedes albopictus.Kata kunci : Ovitrap, Indoor, Outdoor dan Gampong Pineung.ABSTRACTThis study aims to determine the presence and distribution of Aedes spp mosquitoes as a vector of dengue hemorrhagic fever (DHF) using ovitrap in Gampong Pineung area, Syiah Kuala subdistrict both indoor (indoor) and outside (outdoor). The research was conducted in five sub-villages in Gampong Pineung, Syiah Kuala sub-district, namely: Tgk Chik Dipineung, Bintara Pineung, Tgk Hasyim, Tgk Tengoh, and T Muda Rayeuk. The observation of total Aedes spp mosquito eggs in the five hamlets did not show a significant difference (P 0.05). While the mean total number of indoor and outdoor mosquito eggs between hamlets there was a real difference (P 0,05). But on observation of the average number of Aedes agypti larvae on indoor space was significantly different (P 0.05) compared with Aedes albopictus larvae. Similarly, the average number of Aedes agypti larvae in outdoor spaces was significantly different (P 0.05) compared with the Aedes albopictus larvae.Keyword : Ovitrap , Indoor, Outdoor, and Gampong Pineung.
EKSPRESI INSULIN PADA PANKREAS MENCIT (Mus musculus) YANG DIINDUKSI DENGAN STREPTOZOTOCIN BERULANG Erwin E; Etriwati E; Muttaqien M; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.123 KB) | DOI: 10.21157/j.ked.hewan.v7i2.900

Abstract

Penelitian ini bertujuan mengetahui ekspresi insulin pada pankreas mencit (Mus musculus) yang diinduksi streptozotocin berulang dengan pewarnaan imunohistokimia yang berguna sebagai hewan model diabetes melitus. Tiga puluh ekor mencit jantan galur Balb-C, umur 12-14 minggu dengan bobot badan 30-40 g dikelompokkan menjadi 2 kelompok perlakuan, masing-masing kelompok terdiri atas 15 ekor. Kelompok 1 (K1) diberikan pelarut streptozotocin, sedangkan kelompok 2 (K2) diberikan streptozotocin dengan dosis 40 mg/kg bobot badan dalam 50 mM natrium sitrat bufer pH 4,5 secara intraperitoneal sebanyak 0,5 ml selama 5 hari berturut-turut. Hewan percobaan dari masing-masing kelompok dieutanasia sebanyak 2 ekor pada hari ke-0, 7, 14, 21, dan 28 setelah perlakuan, selanjutnya mencit diperfusi dan dinekropsi untuk mengambil jaringan pankreas sebagai sampel pemeriksaan imunohistokimia dengan metode streptavidin peroksidase menggunakan antibodi mouse anti-insulin (1:300). Berdasarkan uji statistik menggunakan analisis varian, ekspresi insulin pada sel beta Langerhans pankreas K1 lebih tinggi dibandingkan K2 (P0,05). Waktu pengamatan dan interaksi antara kelompok dan waktu pengamatan menunjukkan perbedaan yang signifikan (P0,05). Induksi dosis rendah streptozotocin secara berulang dapat menurunkan jumlah ekspresi sel beta Langerhans pankreas yang imunoreaktif terhadap insulin.
EKSPRESI INSULIN PADA PANKREAS MENCIT (Mus musculus) YANG DIINDUKSI DENGAN STREPTOZOTOCIN BERULANG Erwin E; Etriwati E; Muttaqien M; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.123 KB) | DOI: 10.21157/j.ked.hewan.v7i1.899

Abstract

Penelitian ini bertujuan mengetahui ekspresi insulin pada pankreas mencit (Mus musculus) yang diinduksi streptozotocin berulang dengan pewarnaan imunohistokimia yang berguna sebagai hewan model diabetes melitus. Tiga puluh ekor mencit jantan galur Balb-C, umur 12-14 minggu dengan bobot badan 30-40 g dikelompokkan menjadi 2 kelompok perlakuan, masing-masing kelompok terdiri atas 15 ekor. Kelompok 1 (K1) diberikan pelarut streptozotocin, sedangkan kelompok 2 (K2) diberikan streptozotocin dengan dosis 40 mg/kg bobot badan dalam 50 mM natrium sitrat bufer pH 4,5 secara intraperitoneal sebanyak 0,5 ml selama 5 hari berturut-turut. Hewan percobaan dari masing-masing kelompok dieutanasia sebanyak 2 ekor pada hari ke-0, 7, 14, 21, dan 28 setelah perlakuan, selanjutnya mencit diperfusi dan dinekropsi untuk mengambil jaringan pankreas sebagai sampel pemeriksaan imunohistokimia dengan metode streptavidin peroksidase menggunakan antibodi mouse anti-insulin (1:300). Berdasarkan uji statistik menggunakan analisis varian, ekspresi insulin pada sel beta Langerhans pankreas K1 lebih tinggi dibandingkan K2 (P0,05). Waktu pengamatan dan interaksi antara kelompok dan waktu pengamatan menunjukkan perbedaan yang signifikan (P0,05). Induksi dosis rendah streptozotocin secara berulang dapat menurunkan jumlah ekspresi sel beta Langerhans pankreas yang imunoreaktif terhadap insulin.