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IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM Dwiyani, Rindang; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, Ketut; Mayadewi, Ni Nyoman Ari
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid that has been rare in nature,Â so it needs attention to take care and conserve them.Â The objective of the research was to investigate the effect of coconut water and / or tomato juice on the growth of protocorm of V. tricolor Lindl. var suavis from Bali grown in vitro. The experiment was laid out in the factorial design, with two factors (coconut water/CW and tomato juice/TJ), each contained three concentration (CW: 0, 100, and 200 cc L-1; TJ: 0, 100 and 200 g L-1) resulted in nine combination of treatments and replicated four times.Â The results showed that tomato juice with concentration of 100 gL-1 or 200 gL-1 promotes growth of protocorms of Vanda tricolor Lindl. var. suavis from Bali regardless the presence ofÂ coconut water.Â Keywords: coconut water, in-vitro, protocorms, tomato juice, Vanda tricolorÂ

IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM Dwiyani, Rindang; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, Ketut; Mayadewi, Ni Nyoman Ari
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.497

Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid that has been rare in nature,Â so it needs attention to take care and conserve them.Â The objective of the research was to investigate the effect of coconut water and / or tomato juice on the growth of protocorm of V. tricolor Lindl. var suavis from Bali grown in vitro. The experiment was laid out in the factorial design, with two factors (coconut water/CW and tomato juice/TJ), each contained three concentration (CW: 0, 100, and 200 cc L-1; TJ: 0, 100 and 200 g L-1) resulted in nine combination of treatments and replicated four times.Â The results showed that tomato juice with concentration of 100 gL-1 or 200 gL-1 promotes growth of protocorms of Vanda tricolor Lindl. var. suavis from Bali regardless the presence ofÂ coconut water.

ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY Ida Ayu Putri Darmawati; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3rd and 5th clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.

TEKNIK STERILISASI DAN EFEKTIVITAS 2,4-D TERHADAP PEMBENTUKAN KALUS EKSPLAN DAUN NILAM (Pogostemon cablin Benth) IN VITRO Yuyun Fitriani; Gede Wijana; Ida Ayu Putri Darmawati
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Patchouli plant (Pogostemon cablin Benth) is a plant that produce essential oil which is called patchouli oil. Indonesia is the largest supplier of patchouli oil in the world market, which contribute about 90%. Advantage of patchouli oil in the perfume industry is a fixative property, it has the ability to bind with other oils therefore the scent can last for long time and has yet to be made synthetically. However, it takes very long time to produce essential oils in the conventional production system, becouse the growth of patchouli plant is relative slow. One alternative to produce the raw material or patchouli plant faster is through the tissue culture. The sterilization technique and the growth regulator are main factor in tissue culture. Therefore the purpose of this study was to find a sterilization technique, as wel as the concentration of growth regulators 2,4-D (2,4diclorophenoxy acetic acid) in MS + coconut water medium. Sterilization with HgCl2 mercury chloride) at concentration 0.1% for 10 min was the best method of sterilization to minimize contamination. Plant growth regulators 2,4-D at concentration 0.5 ppm was found very affectivein accelerating the formation of patchouli leaf callus in vitro. The callus formation occured retatively fast (9.67 days) with cream-colour, crumb texture, and with diameter 1.21 cm. The fresh and dry weight of callus were 1.35 g and 0.76 g respectively.

Inisiasi Kalus Embriogenik Stroberi (Fragaria sp.) dengan Pemberian IBA (Indolebutyricacid) dan BAP (Benzylaminopurine) AGUNG WIDYA ANTASARI DEWI; IDA AYU PUTRI DARMAWATI; COKORDA GEDE ALIT SEMARAJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.3, Juli 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Initiation Of Embryogenic Callus Strawberries (Fragaria sp.) By Giving IBA (Indolebutyric Acid) and BAP (Benzylaminopurine)Micropropagation or multiplication method by vegetative that could produce seedling in the high number and in a short time, had the same character as its mains, with a progress that not depends on the weather.This research intend to find the effect of grow regulator IBA (Indolebutyric Acid) and BAP (Benzyl aminopurine) and to find the best combination on producing callus strawberry. This research done in Unit Pelaksanaan Teknis (UPT) kultur jaringan Program Study Agroekoteknologi, Faculty of Agriculture, Udayana University. The materials use by the base of strawberry leaf.Growth regulators IBA and BAP could grown a callus. Usage 0,5 ppm IBA + 0,5 ppm BAP is the most effective in deflexion. Relative fast in deflexion (2,6 days). Usage 1 ppm IBA + 1 ppm BAP is the most effective in expansion (6,3 days) but in 30 days mandating its not grown any callus.It require toned to study the sterilisasion method to minimize the contamination and browning in explants strawberry leaf and a tool that in use to do research more to get the right concentration with IBA and BAP combination to get the callus.

Kajian dan Induksi Tunas Tanaman Anggur Merah (Vitis vinifera L. var. Prabu Bestari) dengan Beberapa Jenis Sitokinin Secara In Vitro KADEK PEBRIYANI; RINDANG DWIYANI; IDA AYU PUTRI DARMAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Study of Sterilization and Induction of Red Grape Shoots (Vitis vinifera L. var. Prabu Bestari) with Several Types of Cytokinins In Vitro This study aims to determine the most suitable sterilization method for the sterilization of red grape explants and to determine the effect of giving growth regulators BAP, 2iP, and TDZ in the induction of red grape shoots. This research was conducted from January to June 2020 at the Tissue Culture Laboratory, Faculty of Agriculture, Udayana University. The design used in this study used a completely randomized design (CRD). The parameters observed in this study were the percentage of contamination, when the shoots appeared, when the roots appeared, the percentage of shoot growth explants, the percentage of root growth explants, and the percentage of leaf growth explants. This research is discussed descriptively. The results of the analysis of the research on the sterilization method showed that the sterilization method I through the maintenance of mother plants with 80% mankozeb and the use of clorox immersion in gradual concentrations was able to reduce the percentage of contamination to 61.2% and the use of TDZ 1 ppm growth regulators in combination with 0.01 ppm NAA on WPM medium was able to induce shoots and roots in red wine explants.

Penggunaan Chrysal untuk Memperpanjang Kesegaran Bunga Potong Mawar (Rosa hybrida L.) DIMAS MANDALA PUTRA; HESTIN YUSWANTI; IDA AYU PUTRI DARMAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Chrysal Use to Extend Vaselife Cut Flowers (Rosa hybrida L.) RosesThe demand for cut roses increasing but the problems that often occur in old rose cut flowers is the vaselife of flowers very short. One way that can be done to prolong the vaselife of flowers adaah by giving a solution of the marinade. The main function of a marinade solution is to maintain the vaselife of cut flowers as long as possible. Chrysal. Chrysal a marinade solution containing sucrose, dextrose and citric acid that can extend the life of flowers (vaselife) Another important factor in maintaining the vaselife of flowers is the availability of water. Water will go faster if the wider cross-section, for example by cutting the plant stem oblique. Objective To know the concentration of the shredded material Chrysal optimized to produce a maximum vaselife of flowers. Knowing the difference in the vaselife of roses stalks in pieces tilt and horizontal concentration Chrysal know the interaction between the treatment of cuts rose to the vaselife of roses. This research was conducted at the Laboratory of Post Harvest Technology Faculty of Agriculture, University of Udayana. This study uses a completely randomized factorial design with two factors. Based on this research, interaction between concentration Chrysal (L) by cutting the flower stalk (A) does not affect significantly (p ? 0.05) of all observed variables. In the single factor cutting flower stalk treatment (A) significant (p> 0.05) to variable interest weight loss, dantotal solution is absorbed, whereas the concentration of Chrysal (L) a very significant effect on the flowers begin to bloom, longer vaselife, and the total solution is absorbed.

Studi Penyimpanan Entres Wani Ngumpen Bali (Mangifera caesia Jack var. Ngumpen Bali) terhadap Keberhasilan Grafting ANAK AGUNG NGURAH DHARMA SEPUTRA; IDA AYU PUTRI DARMAWATI; A.A. MADE ASTININGSIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.1, Januari 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Study on Entres Storage of Wani Ngumpen Bali (Mangifera caesia Jack var. Ngumpen Bali) On Success of Grafting Wani Ngumpen Bali (Mangifera caesia Jack var. Ngumpen Bali) is one of tropical fruit which belongs to genius mangifera. The obstacle that are often faced in propagation of Wani Ngumpen Bali by grafting are the long distance between the mother plant and the nursery location, so it takes time to be grafted. The purpose of this research is to study the scion storage of Wani Ngumpen Bali on success of grafting. The research start from February to April 2021, located in Sudaji Village, Sawan District, Buleleng Regency, Bali. The experimental design used was a randomized block design with a single factor. The factor studied was the length of storage of scion which consisted of 7 levels, namely scion were not stored, scion were stored for 1, 2, 3, 4, 5 and 6 days. The treatment was repeated 4 times with each treatment using 2 plants, so there were 56 experimental units. Parameter observed were the percentage of grafting successfulness, shoot length, and number of leaves formed. The results showed that the grafts that were directly connected had the highest percentage of live connections of 62.5%, followed by those that were stored for one day had a percentage of 50.0% then those that had a two-day shelf life had a percentage of 37.5%. Entres with a shelf life of three to six days failed to grow with a percentage of 0%.

Keragaan Agronomi dan Ketahanan Beberapa Varietas Unggul Padi (Oryza Sativa L.) terhadap Serangan Penyakit Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) di Bali IDA BAGUS KADE SUASTIKA; I MADE RAI YASA; A.A.N.B. KAMANDALU; IDA AYU PUTRI DARMAWATI; NI PUTU SUTAMI; S.A.N. ARYAWATI; I WAYAN SUNANJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.4, Oktober 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Agronomic Performance and Resistance on Several Hight Yielding Varieties (Oryza sativa L.) to Bacterial Lesf Blight (Xanthomonas oryzae pv. oryzae) In Bali Province, Indonesia The purpose of the test was to determine the level of bacterial leaf blight (BLB) in several high yielding varieties to obtain resistant varieties to replace Ciherang. The study was carried out by cultivating several high-yielding rice varieties in rice fields in Subak Dlod Sema, Badung from January to May 2020. The appropriate design was a 1-factor randomized block design with 5 treatments and was repeated 5 times. The superior varieties planted were Inpari 19, Inpari 40, Inpari blast, Situ Bagendit and Ciherang varieties for comparison. The incidence and severity of BLB were assesed in the field using the diagonal random sampling method by measuring the lenght of the lesions on the leaves. Measurement of plant samples as many as 10 clumps on a natural plot measuring 400 m2 diagonally. Measurement of BLB disease attack is carried out every 2 weeks starting from the age of the plant 2 weeks after planting (WAP) to 10 WAP. Analysis of the data using Analysis of Variance (ANOVA) and continued by testing the mean value of BNT at 5% level. To see the progression of the severity of BLB disease, it was analyzed by regression. The results of the study showed that 2 of the 4 varieties studied, namely Inpari 40 and Inpari blas were not attacked by BLB disease with an attack of 0.71% each while Inpari 19 and Situ Bagendit were attacked by HDB disease with a mild attack category, namely 2.67% and 2.97% compared to 3.20%. The increasing time of disease progression increased in Inpari 19 and Situ Bagendit with the regression equation y= 0.0464x²+3877x and y = 0.0275x2+0.38x compared to y = 0.0645x²+0.2935x. The higher the number of tillers/clumps, the number of filled grain/panicles and the number of grain/panicles can increase yield production. The production losses of Inpari 40 and Inpari blast were 0.71% each, so it can be said that the introduction of high yielding varieties (HYV) is feasible to be developed as a substitute for Ciherang in the use of resistant varieties to control BLB disease.

Induksi Propagul Kultur Nodus Tanaman Anggur (Vitis vinifera) varietas Prabu Bestari pada Berbagai Konsentrasi ZPT BA dan NAA DINI WIRDASARI; RINDANG DWIYANI; IDA AYU PUTRI DARMAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.1, Januari 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Propagule Induction of Node Culture of Grape (Vitis vinifera) varieties Prabu Bestari at Various Concentration of BA and NAA Propagation of grape seeds still uses conventional methods such as cuttings and grafting. In vitro culture technology can be a solution for plant propagation techniques that using small explants and in small quantities but capable to producing large amounts of seeds. Propagules are new formations that arise from explant tissue, can be in the form of shoots or callus that can occur through direct or indirect organogenesis. The aim of the research was to obtain the best culture medium in the formation of propagules. The research was conducted in two stages, the first was propagule induction and the second was subculture. The research used a Completely Randomized Design. Propagule induction was used a factor with combination of BA and NAA. The levels use of ZPT BA are: 2 ppm, 4 ppm, 6 ppm and the levels use of ZPT NAA are: 1 ppm, 3 ppm, and 5 ppm. The total combination treatment was 9 treatments and the addition of treatment K0 (control). Each treatment was repeated 3 times for a total of 30 experimental units. The subculture medium used was WPM + 1 ppm TDZ. The results showed that MS treatment with a combination of ZPT BA and NAA succeeded in forming propagules in the form of meristemoids and shoots. K3 (MS + 2 ppm BA + 5 ppm NAA) was the treatment that gave the best response to the percentage of meristemoid formation of 100%. K4 (MS + 4 ppm BA + 1 ppm NAA) was the treatment that succeeded in inducing 33.3% shoots.

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