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All Journal Jurnal Hortikultura Indonesia Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) AGRIVITA, Journal of Agricultural Science Nandur
Ida Ayu Putri Darmawati
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science

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Journal : Journal of Agricultural Science and Biotechnology

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TEKNIK STERILISASI DAN EFEKTIVITAS 2,4-D TERHADAP PEMBENTUKAN KALUS EKSPLAN DAUN NILAM (Pogostemon cablin Benth) IN VITRO Yuyun Fitriani; Gede Wijana; Ida Ayu Putri Darmawati
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Patchouli plant (Pogostemon cablin Benth) is a plant that produce essential oil which is called patchouli oil. Indonesia is the largest supplier of patchouli oil in the world market, which contribute about 90%. Advantage of patchouli oil in the perfume industry is a fixative property, it has the ability to bind with other oils therefore the scent can last for long time and has yet to be made synthetically. However, it takes very long time to produce essential oils in the conventional production system, becouse the growth of patchouli plant is relative slow. One alternative to produce the raw material or patchouli plant faster is through the tissue culture. The sterilization technique and the growth regulator are main factor in tissue culture. Therefore the purpose of this study was to find a sterilization technique, as wel as the concentration of growth regulators 2,4-D (2,4diclorophenoxy acetic acid) in MS + coconut water medium. Sterilization with HgCl2 mercury chloride) at concentration 0.1% for 10 min was the best method of sterilization to minimize contamination. Plant growth regulators 2,4-D at concentration 0.5 ppm was found very affectivein accelerating the formation of patchouli leaf callus in vitro. The callus formation occured retatively fast (9.67 days) with cream-colour, crumb texture, and with diameter 1.21 cm. The fresh and dry weight of callus were 1.35 g and 0.76 g respectively.
Co-Authors A.A.N.B. KAMANDALU AGUNG WIDYA ANTASARI DEWI ANAK AGUNG MADE ASTININGSIH ANAK AGUNG NGURAH DHARMA SEPUTRA Cokorda Gede Alit Semarajaya DIMAS MANDALA PUTRA DINI WIRDASARI Gede Wijana Gusti Ngurah Alit Susanta Wirya H. Yuswanti HESTIN YUSWANTI I GUSTI ALIT GUNADI I Ketut Sardiana I MADE AGUS KRISNA I MADE RAI YASA I NYOMAN RAI I PUTU DIAN PRATAMA I WAYAN SUNANJAYA I.G.A. Gunadi IDA AYU ASTARINI IDA AYU MAYUN Ida Ayu Sri Dewi Adriani IDA BAGUS KADE SUASTIKA K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT SUADA Ketut Suada N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI NENGAH DARMIATI NI NYOMAN ARI MAYADEWI NI PUTU SUTAMI NI WAYAN SURIANTARI PUTRI REZKY Retno Kawuri Rindang Dwiyani S.A.N. ARYAWATI TRISNA AGUNG PHABIOLA Y. Fitriani Yuyun Fitriani