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All Journal Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus

I Wayan Gorda

Laboratorium Bedah Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB Sudirman, Denpasar, Bali, Indonesia 80225.

Author-ID : 270097

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Penambahan Astaxanthin pada Pengencer Kuning Telur Berbagai Jenis Unggas Dapat Memproteksi Semen Babi Selama Penyimpanan (THE ADDITION OF ASTAXANTHIN ON SPERM DILUENTS PHOSPHATE EGGYOLK OF VARIOUS POULTRY CAN PROTECT QUALITY OF PIG SPERM DURING STORAGE) Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was conducted to formulate semen diluent of pigswith a better quality, cheap and easy toprepare using egg yolk of various poultries such as chickens, ducks and quails in combinationwithastaxanthin, a potent antioxidant. The research design used was a completely randomized factorialdesign with three different types of sperm diluents and four levels of astaxanthin concentration. Spermdiluents used were phosphate duck egg yolk, phosphate quail egg yolk and yolks phosphate supplementedrespectivelywith 0,002%, 0,004% and 0,008%astaxanthine. The treated sperm were strored at 5oC for 48hours. The sperm qualities were examined for progressive motility, spermatozoa abnormalities, viabilityand plasma membrane integrity. The result showed that sperm diluents of using duck egg yolk phosphatein combination with 0.002% astaxanthinresulted in the highest progressive motility, viability and plasmamembrane intact while abnormalities spermatozoa is lowest. It can be concluded that phosphate duck eggyolk sperm diluents with the addition of 0,002% astaxanthinappeared to be able to maintain the qualityof pig sperm stored at 5oC for 48 hours.

Laporan Kasus: Obstruksi Rektum dan Uretra Akibat Fibroma Serviks pada Anjing Golden Retriever (RECTAL AND URETRA OBSTRUCTION DUE TO FIBROMA CERVICES IN GOLDEN RETRIEVER DOG: CASE REPORT ) I Nyoman Suartha; I Wayan Wirata; I Wayan Gorda; Agatha Serena Lumban Tobing; I Ketut Eli Supartika
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Rectal obstruction in dogs is generally caused by foreign objects such as bone or hard feces, whereas urethral obstruction is generally caused by calculi. Cases of rectal and urethral obstruction due to tumors or neoplasia in the female reproductive tract of dogs are rare. Fibroma is one type of benign tumor that attacks fibroblast tissue. The formation of the tumor results in obstruction of the gastrointestinal tract and urine. This paper aims to raise cases of rectum obstruction and vesica urinaria in dogs. Fourth yearold female golden retriever dogs was admitted to Veterinary Hospital Faculty of Veterinary Medicine Udayana University. According to the case history of owner, the dog was suffered weakness, anoreksia, bally and tend. The dog was difficult to urinate and poo. The stool was suppressed like “ornament cake birth day”. All of health condition were normal. Clinical examination revealed presence mass like tumor attached to the cervix with consistence hard and irregular form. Ultrasonography examination was distended bladder without calculi form. Hematological routine have leucophilia, anemia and thrombositopenia. After general anesthesia with ketamine HCl and xylazine, the mass with irregular form and hard consistence was surgical removed. Histophatological section were revealed presence of fibroma which represented by arrangement of cell that have feature tapering cell as fibroblast without mitosis. The mass was diagnosed as fibroma. It concluded that golden retriever dogs had obstruction of rectum and vesica urinaria as result of cervical fibroma tumors.

LAKTOSA-ASTAXANTHIN MENINGKATKAN KUALITAS SEMEN BEKU KAMBING GEMBRONG DALAM UPAYA KONSERVASI Wayan Bebas; Wayan Gorda; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Tjok Gede Oka Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research was conducted to produce a formula of diluent for the manufacture of frozen semen of Gembrong goat. Yolk phosphate was used as a basic diluent with the addition of anti-cold shock, antioxidants and combination of anti-cold shock-antioxidant. The research design used a completely randomized design with three kinds of treatments; T1: the addition of lactose 0.6% (anti-cold shock), T2: the addition of astaxanthin 0.004% (antioxidant), and T3: a combination of 0.6% lactose-Astaxanthin 0.004% (combination of anti-cold shock and antioxidants). The addition of DMSO 6% was used in each treatment as intracellular cryo protectants. The freezing process was done with the gradual freezing with conventional techniques. Examination of the quality of semen by thawing prior included progressive motility, viability, and abnormalities. The results showed that anticoldshock-antioxidant combination (0.6% lactose -Astaxanthin 0.004%) produced the best semen quality with progressive motility, viability, and less abnormalities as follow 44.00 + 3.46%, 59.00 + 1.85%, and 14.00 + 0.76% respectively. It was concluded that the addition of a combination of 0.6% lactose-Astaxanthin 0.004% on the phosphate yolk diluent with 6% DMSO as intracellular produced best quality of frozen goat Gembrong semen that suitable for use in artificial insemination and in vitro fertilization.

Kadar Krioprotektan Gliserol dan Dimethylsulfoxide Terbaik pada Pengencer Astaxanthin Fosfat Kuning Telur Bebek Terhadap Kualitas Semen Beku Babi Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study to examine the addition of cryoprotectant glycerol and dimethylsulfoxide (DMSO) in astaxanthin phosphate duck egg yolk on the quality of frozen boar semen. Each of the cryoprotectants distinguished its concentration ie : 4%, 6%, 8%, 10%, and 12% for freezing of semen. Semen was collected from one Landrace boar then performed macroscopically and microscopically. Semen was divided into 10 tubes each diluted with the duck egg yolk astaxanthin phosphate extender diluent then stored at 20oC for 2 hours, centrifugation for 15 minutes (2000 rpm) then taken the pellet with 1 ml of supernatant. The pellets were diluted with astaxanthin phosphate duck egg yolk extender containing various concentration (4, 6, 8, 10, and 12%). Semen was packed with 0.5 mL straw and performed equilibration for 2 hours (4oC). do aeration above liquid N2 vapor for 10 minutes, then dipped in liquid N2. Semen was stored in liquid nitrogen container for 24 hours , and then quality of spermatozoa was evaluated post thawing. The results of the study showed that addition of cryoprotectan glycerol and DMSO were no difference significant (p>0,05) on the quality of frozen semen, but cryoprotectant concentrations (4%, 6%, 8%, 10%, and 12%) significantly affected (p <0.05) the quality of frozen semen. Glycerol with a concentration of 4% produces the best quality frozen semen, whereas DMSO with a concentration of 6% produces the best quality frozen semen.

Penambahan Bovine Serum Albumin pada Beltsville Thawing Solution Dapat Mempertahankan Kualitas Semen Babi yang Disimpan pada 15°C (ADDITION OF BOVINE SERUM ALBUMIN TO BELTSVILLE THAWING SOLUTION COULD MAINTAIN QUALITY OF PIG SEMEN STORED AT 15OC) Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to maintain the quality of pig semen for longer during storage at 15oC, in an effort to support artificial insemination programs with the addition of Bovine Serum albumin (BSA) to diluent Beltsville Thawing Solution (BTS). This study uses a completely randomized design with five treatment groups, each To = semen was diluted with BTS without the addition of BSA ; T1 = with the addition of 5 mg BSA/mL diluent; T2 = with the addition of 10 mg BSA/mL diluent; T3 = with the addition of 15 mg BSA/mL diluent; T4 = with the addition of 20 mg BSA/mL diluent. Each treatment was repeated five times so that the number of samples used was twenty-five. The diluted cement is stored at 15oC for 72 hours then observing the quality of cement includes: progressive motility, dead spermatozoa, abnormalities, and intact plasma membranes. The data obtained were analyzed by analysis of variance, if there were differences followed by Duncan’s test. The results showed, addition of BSA concentration of 10 mg/mL and 15 mg/mL of diluent gives the same effect on the quality of cement during storage and significantly better (p <0.05) when compared to the addition of 0 mg/mL, 5 mg/mL and 20 mg/mL diluents. It can be concluded, the addition of BSA 10 mg/mL BTS diluents can maintain the most optimal quality of pig semen against progressive motility, dead spermatozoa, abnormalities and intact plasma membranes.

Tepung Tempe Kaya Isoflavon Meningkatkan Kadar Kalsium, Posfor dan Estrogen Plasma Tikus Betina Normal I Nyoman Suarsana; I Nyoman Sadra Dharmawan; I Wayan Gorda; Bambang Pontjo Priosoeryanto4
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research aimed to study the effect of isoflavon-riched tempe flour on calcium (Ca, phosphate(P), and estrogen levels in plasma et normal female rats during their growth period. A level oftwenty-five 2 months old female Sprague Dawley rats with an avarage body weight of 200 g wasrandomly divided into into 5 groups: one with control group (KO: without treatment) and fourtreatment groups (K1, K2, K3, K4 : animals were given tempe flour with isoflavon at 1; 2; 4; and 6 mg/200 g/bw, respectively). The treatment was conducted for two months, following this blood plasmawas collected to analyse the level of calcium, phosphor, and estrogen, respectively. The resultshowed that although the plasma level of Ca, P, and estrogen was higher in the treatment groupcompare to the control group, this was not significantly different (P>0,05). The highest plasmalevel of Ca, P, and estrogen was seen in anmal receiving tempe flour with 4 mg/ 200 g bw/dayisoflavon.

Laporan Kasus: Pyometra Pada Kucing Lokal I Made Agus Adnyana; I Wayan Gorda; I Gusti Ngurah Sudisma
Buletin Veteriner Udayana Vol. 14 No. 4 August 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Pyometra is a hormonal disorder that causes infection so that pus accumulates in the uterus, abscesses are usually caused by excess hormone progesterone, where the hormone progesterone causes thickening of the uterine wall. Pyometra is commonly found in dogs or cats, but is more common in dogs. A cat is 9 months old, weighs 1.95 kg and is female. Checked at the Animal Hospital, Faculty of Veterinary Medicine, Udayana University with complaints, the presence of runny out of animal genitals. The results of physical examinations that appear to come out of the animal's genitals, in the form of pus and stomach, look big like pregnant women. From hematological examination showed an increase in Hemoglobin 17.9 g / dL, reference values ??8.0 - 15.0 g / dL, and Mean Corpuscular Hemoglobin Concentration 41.4 g / dL reference values ??30.0 - 36.0 g / dL because animals lack oxygen so the hemoglobin level increases. Handling of Pyometra can be done by removing the uterus and ovary (ovariohysterectomy). Seven days postoperatively, the patient was declared cured and healthy again, with incisional wounds fused and no pus flowing out of the animal's genitals.

Co-Authors A.A. Gde Jaya Wardita Agatha Serena Lumban Tobing Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Kendran Asih, Ni Wayan Nicky Astrin, Baiq Nia Rolyana Bambang Pontjo Priosoeryanto Bendelin Melda Loekali Desak Nyoman Dewi Indira Laksmi Gusti Ayu Yuniati Kencana I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Ketut Anom Dada I Ketut Eli Supartika I Made Agus Adnyana I Made Kardena I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Putu Cahyadi Putra, I Putu Cahyadi I Wayan Bebas I Wayan Wirata Ida Bagus Oka Winaya Jayawardhita, Anak Agung Gde Kamaliani, Baiq Renny Komang Sri Gilang Airlangga Laksmi, I Gusti Ayu Indira Luh Dewi Anggreni Luh Made Sudimartini Naomi, Carene Ni Putu Trisna Asih Pratistha, Ni Wayan Apsari Shantika Putri, Rizki Kusuma Rosita, Indah Sultan, Nurul Fadillah Tjok Gede Oka Pemayun Warditha, Anak Agung Gde Jaya Wayan Herry Gumawan

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