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All Journal Bioscience Biota Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Pelita Eksakta Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks TROPICAL GENETICS Tropical Genetics
Elsa Badriyya
Fakultas Farmasi, Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : TROPICAL GENETICS

 1 
Design of primer Ipomoea batatas chloroplast gene matK S. Syamsurizal; Ardi Ardi; Des M; Resti Fevria; Yusni Atifah; Elsa Badriyya; Afifatul Achyar
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Sweet potato varieties (Ipomoea batatas) Pucuk Hitam Panyalaian and Madu Pucuk Hitam Panyalaian have the potential to be a superior commodity in West Sumatra, but the information of genetic diversity is very limited. Data on the diversity of sweet potato germplasm is beneficial in the selection of plants to obtain superior cultivars in plant breeding. Aim: The aim of the study was to find specific primers for the study of sweet potato genetic markers using Ipomoea batatas chloroplast genes matK. Methods: DNA extraction from sweet potato young leaves; designing forward and reverse primers Ipomoea batatas chloroplast genes matK; PCR; sequencing; bioinformatics analysis and species identification by comparing the NCBI database. Results: Plant DNA barcoding PCR using designed primers matK were successfully resulting single DNA band in different amplicon size in some samples. This indicating that the designed primers used were able to distinguish variation in one species.
Pedigree analysis of diabetes mellitus in Minangkabau ethnic S. Syamsurizal; Siti Halifah; Elsa Badriyya
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Diabetes mellitus has a tendency to be inherited. The inheritance pattern of diabetes mellitus in the Minangkabau ethnic group follows the mitochondrial and autosomal inheritance pattern. Patients with diabetes mellitus in the Minangkabau ethnicity have a equal distribution between men and women. Patients with diabetes mellitus in this ethnic group generally know that they suffer from diabetes mellitus in old age or after other complications arise due to diabetes mellitus.
Literature Study : Chromosomal Changes in The Class Mammalia Genus Neacomys, Class Actinopterygii Genus characidium, Class Reptile Family Boidae Nindya Ananda Latifa; Sintia Putri; Elsa Badriyya
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i1.24

Abstract

Chromosomes become an important substance for an organism because they contain information useful for cell regulation and as a hereditary agent. The character of an organism is determined by genes located on chromosomes. Changes in chromosomes will have a significant influence on the evolution of a species. One of the factors that influence changes in chromosomes is mutation. Mutation is a form of defense of an organism in the face of the changing state of nature. Neacomys diversification is caused by gene fusion events, translocations, amplications, and constitutive heterochromatin (CH). In the family Crenuchidae genus Caracidium there was a revision in the diversification of chromosomes 2n of 50 chromosomes composed of 32 metacentric + 18 sub-metacentrics. This is due to differences in the number of rDNA sites, heterochromatin blocks, number of B chromosomes and the size of sex chromosomes, as well as repeated DNA dynamics on chromosomes observed among species forming diversification and chromosomal speciation. In the boidae family reptile class there was a fusion of chromosomes which resulted in the conclusion that the kariotype configuration in snakes is currently the clade of primitive Neotropic snakes.
Review Article: SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) as a Metabolic Disorder Genetic Marker Elsa Badriyya; Siti Fatimah Putri Hasyul
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.26

Abstract

A metabolic disorder appears when unusual chemical reactions impair the metabolism process in the body. This condition affects how the body distributes and processes macronutrients, including protein, lipids, and carbohydrates. Transcription Factor 7 Like 2 (TCF7L2) is a 217,460 bp gene, found in chromosome 10q25, and encodes the High Mobility Group (HMG) box with a role in the Wnt signaling pathway. The Wnt signaling pathway involves several processes during development, healthy homeostasis, and illness. The reactions that follow the pathway's activation regulate both proliferation and differentiation. Single Nucleotide Polymorphism (SNP) in the TCF7L2 gene has been linked to a variety of metabolic disorders incidence. SNP rs11196205 was found to have a significant association with type 1 and type 2 diabetes mellitus, gestational diabetes mellitus, glucose homeostasis, obesity-related parameters, and renal function. Mechanism SNPs causing the metabolic disorder are still unexplained, but SNPs are suspected to affect transcription and splicing processes. SNPs of TCF7L2 genes are reported to affect proinsulin conversion to insulin, decrease incretin hormone production, and decrease insulin sensitivity throughout the body. It can conclude that the screening for the SNP can be a sign of metabolic process abnormalities, especially in diabetes Mellitus.
Unique Pattern of Hypertension Fingerprints S. Syamsurizal; Sudarni; Rapidah; Elsa Badriyya; Sintia Putri
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.27

Abstract

The fingerprint pattern (dermatoglyphics) of each individual is not the same. Fingerprint patterns are often used as an identification tool in criminal cases and disease diagnosis. Fingerprint patterns for health diagnosis are usually diseases caused by genetic factors, for example hypertension. The purpose of this study was to describe the characteristics of fingertip dermatoglyphics of hypertensive patients. The research sample was 60 people consisting of 30 people with hypertension who visited the DR. M. Djamil Padang and controls 30 non-hypertension (normal) people from Biology FMIPA UNP students. Simple random sampling. The research variables are tendril patterns on the fingertips, the number of tendrils on the fingertips and the atd angle on the palms. The Chi-square test was used to analyze the tendril pattern data of hypertension patients. To compare the number of tendrils and the angle atd, a t-test was used. The results showed that the arch fingerprint pattern is a unique character in hypertensive patients.
Co-Authors Afifatul Achyar Afifatul Achyar Ardi Ardi Atifah, Yusni Des M Dian Ayu Juwita Dwi Hilda Putri Hengki Saputra Indra Hartanto Lailaturrahmi Lailaturrahmi Lailaturrahmi Lailaturrahmi Linda Advinda Nindya Ananda Latifa Rapidah Resti Fevria Resti Fevria S. Syamsurizal S. Syamsurizal Sa`diatul Fuadiyah Sa’diatul Fuadiyah Sintia Putri Siti Fatimah Putri Hasyul Siti Fatimah Putri Hasyul Siti Halifah Sudarni