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All Journal Bioscience Biota Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Pelita Eksakta Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks TROPICAL GENETICS Tropical Genetics
Elsa Badriyya
Fakultas Farmasi, Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Biota

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Primers Designed For Amplifying TCF7L2 Gen S. Syamsurizal; Hengki Saputra; Dwi Hilda Putri; Elsa Badriyya
Biota Vol 6 No 2 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/10.19109/Biota.v6i2.5871

Abstract

Diabetes Mellitus (DM) is a disease characterized by high glucose levels because of the lack of insulin hormone in the body. Prevalence of Type-2 Diabetes Mellitus is associated with the variant of the TCF7L2 gene polymorphism, or SNP. The purpose of this study was to construct a specific primer to amplify the TCF7L2 gene fragment and their capabilities to detect the targeted area. This research was a descriptive study that design the primers using Geneious software version 5.4.7. The study was conducted at the Laboratory of Genetics and Biotechnology FMIPA Universitas Negeri Padang. Based on this research, a 10qF forward primer (NG_012631.1) and 10qR reverse primers (NG_012631.1) has successfully designed. The primers can amplify the targeted area of the TCF7L2 gene with 1208 bp length. The amplified gene region has many variants that are strictly related to the appearance of Type 2 Diabetes Mellitus.
Co-Authors Afifatul Achyar Afifatul Achyar Ardi Ardi Atifah, Yusni Des M Dian Ayu Juwita Dwi Hilda Putri Hengki Saputra Indra Hartanto Lailaturrahmi Lailaturrahmi Lailaturrahmi Lailaturrahmi Linda Advinda Nindya Ananda Latifa Rapidah Resti Fevria Resti Fevria S. Syamsurizal S. Syamsurizal Sa`diatul Fuadiyah Sa’diatul Fuadiyah Sintia Putri Siti Fatimah Putri Hasyul Siti Fatimah Putri Hasyul Siti Halifah Sudarni