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All Journal Bioscience Biota Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Pelita Eksakta Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks TROPICAL GENETICS Tropical Genetics
Elsa Badriyya
Fakultas Farmasi, Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Primer Construction to detect SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) Using Amplification Refractory Mutation System (ARMS) PCR to detect Type-2 Diabetes Mellitus Elsa Badriyya; Afifatul Achyar
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108497-0-00

Abstract

Type-2 Diabetes Mellitus (T2DM) is a metabolic disease characterized by an increase in blood glucose levels. The macrovascular and microvascular complications of T2DM can increase the risk of death in patient. SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) gene has been associated with T2DM in several regions like Iceland, Denmark, the United State, and Thailand. SNP rs11196205 is characterized by polymorphism at position 113.047.288 from nucleotide Guanine to Cytosine. Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS PCR) is a method that can be used to detect mutation or polymorphism in a DNA. The aim of this research was to design a specific primer to detect SNP rs11196205 TCF7L2 gene using ARMS PCR. The design of study was a descriptive study to determine the specific primer. The method of the research includes DNA isolation, primer design using Geneious software, amplification of targeted area using ARMS PCR, and DNA sequencing method for bioinformatics study. Based on the research, four primers to detect SNP rs11196205 TCF7L2 gene have been successfully constructed. The primers were rs11196205-F, rs11196205-R, rs11196205-F(C) as a specific primer for C allele, and rs11196205- R(G) to detect G allele, the reaction produced 3 fragments of 856bp, 559bp, and 339bp.  
Making and Presentation of "Mooghurt" as a Healthy Drink to Improve Health Quality S. Syamsurizal; Linda Advinda; Resti Fevria; Indra Hartanto; Elsa Badriyya
Pelita Eksakta Vol 3 No 1 (2020): Pelita Eksakta Vol. 3 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol3-iss1/120

Abstract

Mooghurt is a trademark of fermented cow's milk similar to yoghurtyang made by the KWU unit of the Student Association majoring in Biology FMIPA UNP. Mooghurt is a biotechnological product of fermented milk that is beneficial for improving the health of the digestive tract, reducing cholesterol in the blood, and stimulating the nervous system. Fresh cow's milk as the main raw material for mooghurt is obtained from the city of Padang Panjang. Padang Panjang is one of the centers of good quality cow's milk production in West Sumatra. Cow dairy farm products in Padang Panjang provide business opportunities for the surrounding community, including the academics of Biology FMIPA UNP in increasing student entrepreneurial spirit. The process of making Mooghurt consists of: 1) making a starter; 2) making mooghurt; 3) manufacture fruit extracts or jellies to complement the presentation.
“SIPODEH” Instant Red Ginger to Trigger Student Entrepreneurial Spirit S. Syamsurizal; Sa’diatul Fuadiyah; Elsa Badriyya
Pelita Eksakta Vol 4 No 2 (2021): Pelita Eksakta Vol. 4 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol4-iss2/160

Abstract

West Sumatra is one of the largest ginger (Zingiber Officinale Rosc) producing provinces in Indonesia. The amount of ginger production in West Sumatra is increasing from year to year. In 2018 the total production of ginger in West Sumatra was estimated at 3,177,549 tons and in 2019 it increased to 4,508,462 tons. Ginger is one of the spice plants in Indonesia which is quite popularly used as traditional medicine. Ginger has a distinctive spicy taste, so it is quite often used as a drink to warm the body (to reject the wind). Nowadays, apart from white ginger which is generally consumed by the public, it turns out that there is another type of ginger which has more properties and a stronger spicy taste, namely red ginger (Zingiber officinale). Red ginger is predicted to be able to inhibit the infection process of the SARS-CoV-2 virus in human host cells and is predicted to be a good oral drug drink. Sudanese people who regularly drink red ginger do not get the flu / COVID-19 and in people who show symptoms of being infected with the corona virus, the symptoms are felt to be mild and recover quickly. This is because red ginger has been shown to increase immunity, increase IgM levels and reduce circulation of pro-inflammatory cytokines. The Biology student association already has a KWU (entrepreneurial) unit. The KWU unit has been engaged in the sale of food products and stationery which did not receive income during the Covid-19 pandemic. To overcome health problems during the Covid-19 pandemic and the business of the KWU HMJ Biology unit which was not optimal, an instant red ginger production business was developed.
Case Study: PPUPIK Mooghurt Team's Marketing Capabilities, Department of Biology, Faculty of Mathematics and Natural Sciences, UNP Elsa Badriyya; S. Syamsurizal; Linda Advinda; Resti Fevria
Pelita Eksakta Vol 3 No 2 (2020): Pelita Eksakta Vol. 3 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol3-iss2/130

Abstract

PPUPIK Mooghurt is a business unit that is part of the Entrepreneurship Unit of the Student Association majoring in Biology, FMIPA UNP. To improve the performance of the PPUPIK Mooghurt business unit, a more reliable marketing strategy is needed. The purpose of PKM PPUPIK Mooghurt: to find out the marketing capability of the KWU HMJ Biology FMIPA UNP unit team and offer solutions to their problems. The marketing ability of the PPUPIK Mooghurt team in general has not been satisfactory, so solutions are given to carry out training and socialize mooghurt products to the public. The results obtained were an increase in marketing capabilities for the PPUPIK Mooghurt team, and the product became better known in the community because teachers were also able to disseminate information to their students.
Primers Designed For Amplifying TCF7L2 Gen S. Syamsurizal; Hengki Saputra; Dwi Hilda Putri; Elsa Badriyya
Biota Vol 6 No 2 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/10.19109/Biota.v6i2.5871

Abstract

Diabetes Mellitus (DM) is a disease characterized by high glucose levels because of the lack of insulin hormone in the body. Prevalence of Type-2 Diabetes Mellitus is associated with the variant of the TCF7L2 gene polymorphism, or SNP. The purpose of this study was to construct a specific primer to amplify the TCF7L2 gene fragment and their capabilities to detect the targeted area. This research was a descriptive study that design the primers using Geneious software version 5.4.7. The study was conducted at the Laboratory of Genetics and Biotechnology FMIPA Universitas Negeri Padang. Based on this research, a 10qF forward primer (NG_012631.1) and 10qR reverse primers (NG_012631.1) has successfully designed. The primers can amplify the targeted area of the TCF7L2 gene with 1208 bp length. The amplified gene region has many variants that are strictly related to the appearance of Type 2 Diabetes Mellitus.
Design of primer Ipomoea batatas chloroplast gene matK S. Syamsurizal; Ardi Ardi; Des M; Resti Fevria; Yusni Atifah; Elsa Badriyya; Afifatul Achyar
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (911.191 KB)

Abstract

Sweet potato varieties (Ipomoea batatas) Pucuk Hitam Panyalaian and Madu Pucuk Hitam Panyalaian have the potential to be a superior commodity in West Sumatra, but the information of genetic diversity is very limited. Data on the diversity of sweet potato germplasm is beneficial in the selection of plants to obtain superior cultivars in plant breeding. Aim: The aim of the study was to find specific primers for the study of sweet potato genetic markers using Ipomoea batatas chloroplast genes matK. Methods: DNA extraction from sweet potato young leaves; designing forward and reverse primers Ipomoea batatas chloroplast genes matK; PCR; sequencing; bioinformatics analysis and species identification by comparing the NCBI database. Results: Plant DNA barcoding PCR using designed primers matK were successfully resulting single DNA band in different amplicon size in some samples. This indicating that the designed primers used were able to distinguish variation in one species.
Pedigree analysis of diabetes mellitus in Minangkabau ethnic S. Syamsurizal; Siti Halifah; Elsa Badriyya
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (823.855 KB)

Abstract

Diabetes mellitus has a tendency to be inherited. The inheritance pattern of diabetes mellitus in the Minangkabau ethnic group follows the mitochondrial and autosomal inheritance pattern. Patients with diabetes mellitus in the Minangkabau ethnicity have a equal distribution between men and women. Patients with diabetes mellitus in this ethnic group generally know that they suffer from diabetes mellitus in old age or after other complications arise due to diabetes mellitus.
STUDENT ACHIEVEMENT IN A PHARMACOTHERAPY PRACTICE COURSE: A CASE STUDY AT AN INDONESIAN PHARMACY SCHOOL Lailaturrahmi Lailaturrahmi; Elsa Badriyya
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 9, No 3 (2020): November
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.54653

Abstract

Background: Indonesian pharmacy schools are expected to meet required clinical pharmacy components and the proportion of practical courses according to nationally established standards. This is essential to produce competent Indonesian pharmacists. The implementation of Pharmacotherapy of Gastrointestinal, Respiratory Tract Diseases, and Special Conditions Practice was one of the measures taken to meet this requirement. This case study aims to explore obstacles in Pharmacotherapy of Gastrointestinal, Respiratory Tract Diseases, and Special Conditions Practice.Case discussion: Pharmacotherapy of Gastrointestinal, Respiratory Tract Diseases, and Special Conditions (FAF 314) Practice was conducted using group case study with SOAP (subjective, objective, assessment, plan) worksheet. During the sixth week of practice, a modified OSCE was conducted to assess the learning process. The skills that were assessed included problem identification, problem-solving, drug information service, effective communication, as well as attitude and professionalism. However, the students’ average score in this assessment was about 1-2 of maximum score 3, and the required passing score was 2. Conclusion: The sub-optimal students’ achievement in the mid-term assessment of Pharmacotherapy of Gastrointestinal, Respiratory Tract Diseases, and Special Conditions Practice may be due to the students’ obstacles in understanding the information from literature and showing effective communication skills and professional attitude in drug information provision. To address these issues, further measures such as constructive alignment analysis of this practice, revising practice activities design and allocating adequate time to practice effective communication skills and professional attitude in drug information provision. Keywords: constructive alignment, communication, pharmacotherapy practice, OSCE, SOAP
Literature Study : Chromosomal Changes in The Class Mammalia Genus Neacomys, Class Actinopterygii Genus characidium, Class Reptile Family Boidae Nindya Ananda Latifa; Sintia Putri; Elsa Badriyya
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i1.24

Abstract

Chromosomes become an important substance for an organism because they contain information useful for cell regulation and as a hereditary agent. The character of an organism is determined by genes located on chromosomes. Changes in chromosomes will have a significant influence on the evolution of a species. One of the factors that influence changes in chromosomes is mutation. Mutation is a form of defense of an organism in the face of the changing state of nature. Neacomys diversification is caused by gene fusion events, translocations, amplications, and constitutive heterochromatin (CH). In the family Crenuchidae genus Caracidium there was a revision in the diversification of chromosomes 2n of 50 chromosomes composed of 32 metacentric + 18 sub-metacentrics. This is due to differences in the number of rDNA sites, heterochromatin blocks, number of B chromosomes and the size of sex chromosomes, as well as repeated DNA dynamics on chromosomes observed among species forming diversification and chromosomal speciation. In the boidae family reptile class there was a fusion of chromosomes which resulted in the conclusion that the kariotype configuration in snakes is currently the clade of primitive Neotropic snakes.
Review Article: SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) as a Metabolic Disorder Genetic Marker Elsa Badriyya; Siti Fatimah Putri Hasyul
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.26

Abstract

A metabolic disorder appears when unusual chemical reactions impair the metabolism process in the body. This condition affects how the body distributes and processes macronutrients, including protein, lipids, and carbohydrates. Transcription Factor 7 Like 2 (TCF7L2) is a 217,460 bp gene, found in chromosome 10q25, and encodes the High Mobility Group (HMG) box with a role in the Wnt signaling pathway. The Wnt signaling pathway involves several processes during development, healthy homeostasis, and illness. The reactions that follow the pathway's activation regulate both proliferation and differentiation. Single Nucleotide Polymorphism (SNP) in the TCF7L2 gene has been linked to a variety of metabolic disorders incidence. SNP rs11196205 was found to have a significant association with type 1 and type 2 diabetes mellitus, gestational diabetes mellitus, glucose homeostasis, obesity-related parameters, and renal function. Mechanism SNPs causing the metabolic disorder are still unexplained, but SNPs are suspected to affect transcription and splicing processes. SNPs of TCF7L2 genes are reported to affect proinsulin conversion to insulin, decrease incretin hormone production, and decrease insulin sensitivity throughout the body. It can conclude that the screening for the SNP can be a sign of metabolic process abnormalities, especially in diabetes Mellitus.
Co-Authors Afifatul Achyar Afifatul Achyar Ardi Ardi Atifah, Yusni Des M Dian Ayu Juwita Dwi Hilda Putri Hengki Saputra Indra Hartanto Lailaturrahmi Lailaturrahmi Lailaturrahmi Lailaturrahmi Linda Advinda Nindya Ananda Latifa Rapidah Resti Fevria Resti Fevria S. Syamsurizal S. Syamsurizal S. Syamsurizal Sa`diatul Fuadiyah Sa’diatul Fuadiyah Sintia Putri Siti Fatimah Putri Hasyul Siti Fatimah Putri Hasyul Siti Halifah Sudarni