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Journal : Eksakta : Berkala Ilmiah Bidang MIPA

ISOLASI KUMARIN DARI KULIT BUAH LIMAU SUNDAI (Citrus nobilis Lour) Melindra Mulia
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 18 No. 02 (2017): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.01 KB) | DOI: 10.24036/eksakta/vol18-iss02/70

Abstract

Coumarin has been isolated and characteritated from rind of Citrus nobilis Lour by maseration methode with methanol. After fractionation by n-hexane and ethyl acetate, collected the phase ethyl acetate which positive of coumarin. From ethyl acetate extract coumarin have been isolated by column chromatography. The isolation results was obtain 2,159 g of pure white needle-shape crystalline with the melting point of 126,2-127,60C. Structure of the isolated coumarine was elucidated by spectroscopic methodes, UV-Vis,13C-NMR, 1H-NMR, 2D-NMR (DEPT/HSQC, COSY, NOESY, HMBC) and IR spectra. Based on the spectra data the isolated coumarine is marmin.
A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera Hesty Parbuntari; Sri Benti Etika; Melindra Mulia; Elfanny Delvia
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 20 No. 2 (2019): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (813.011 KB) | DOI: 10.24036/eksakta/vol20-iss2/193

Abstract

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.
Antioxidant Activities Extracts N-Hexane, Ethyl Acetate and Methanol of Limau Sundai (Citrus nobilis Lour) Peels Melindra Mulia; Mai Efdi; Afrizal Afrizal; Niza Lian Pernadi; Devi Purnamasari
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 23 No. 04 (2022): Eksakta: Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol23-iss04/339

Abstract

The Limau Sundai plant (Citrus Nobilis Lour) is widely known in West Sumatra. The fruit of this plant is commonly consumed as a cooking spice. This antioxidant activity test research aims to find out the potential of Lime Sundai peel as a source of antioxidant compounds. The peel of Limau Sundai fruit is ensnared with methanol solvent and concentrated using a rotary evaporator to obtain a coarse extract. The crude extract is then partitioned successively in n-hexane and ethyl acetate so that n-hexane, ethyl acetate, and methanol extracts are obtained. Sundai lime peel has good antioxidant activity against DPPH, which is shown in the ethyl fraction of acetate, methanol, and liquid methanol. The antioxidant activity of the three fractions is smaller than vitamin C and more significant than the hexane fraction. In the ethyl acetate, methanol and methanol/aqueous fractions with concentrations of 1076, 1822, and 1372 ppm in a row can reduce 50% of DPPH radical activity.