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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Widyariset Widyariset Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences
Michael Haryadi Wibowo
Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Veterinary Other

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Journal : HAYATI Journal of Biosciences

 1 
Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production Niken Respati Maharani; Heru Susetya; Michael Haryadi Wibowo
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.531-539

Abstract

Avian influenza virus H9N2 subtype is considered a low pathogenic strain that has been reported in Indonesia since late 2016. The outbreak has caused economic losses for farmers due to the sharp drop in egg production. The evidence of the existence of AIV H9N2 has been published, but very limited information on the prominent symptom and macroscopic lesion. This research was a retrospective study of suspected avian influenza H9 subtype, obtained from layer commercial farm with recorded characteristic symptoms. Specific trachea samples were collected and further processed to be isolated, propagated using embryonated chicken egg, and then extracted the RNA for molecular detection using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). A positive result was further detected in its H-9 gene with RT-PCR technique and sequencing methods. One of five samples showed positive for RT-qPCR with CT value 30.19. Sequence analysis confirmed that the sample with characteristic macroscopic lesion could be detected in the presence of the AIV H9 subtype. Phylogenetic tree analyses revealed that this virus belongs to the China-Vietnam- Indonesia (CVI) lineage.
Molecular Characterization and Pathogenesis of Newcastle Disease Virus Isolated from Brontok Eagle in West Java, Indonesia Liza Angeliya; Yuli Purwandari Kristianingrum; Widya Asmara; Michael Haryadi Wibowo
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.648-657

Abstract

Newcastle disease (ND) is a contagious disease in poultry and numerous birds of various ages. Eagle is considered a potential reservoir for ND transmission as a wild bird. This research was conducted to molecularly characterize Newcastle disease virus (NDV) isolated from ND cases in Brontok Eagle and analyze the pathogenesis in chicken embryos. qRT-PCR was conducted as confirmation of NDV without mixing Avian Influenza (AI). Sequencing the fusion (F) and haemagglutinin-neuraminidase (HN) genes from the three NDVs was performed with a specific primer. Amino acid sequence compared with other NDV from Genbank. Pathogenicity, genetic variation, distance, and phylogenetic studies were analyzed using bioinformatics software (MEGA-X). This study analyzed pathogenesis based on lesions and distribution of viral antigens in chicken embryos infected with NDV. Observations were based on tissue lesions with HE and IHC staining. NDV isolated from three Brontok Eagles is classified as velogenic strain, virulent NDV (KRQKRF), and belonging to Genotype VII subgenotype VII.2. The NDV was detected in various organ lesions, more severe in the pulmo, trachea, proventriculus, and intestine of chicken embryos. That is still similar to the previous case reports in the field. These results show that NDV, which infected Brontok Eagle, has similar molecular characteristics and pathogenesis in chickens. These cases could be a threat to the poultry industry. Further research, surveillance, and monitoring of wild birds are needed to obtain more NDV epidemiological information in wild birds.
Co-Authors Ade Erma Suryani Aditya Ahkami Pratomo AETH. Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agnesia Endang Trihapsari Wahyuni Agus Eko Srihanto Agus Eko Srihanto, Agus Eko Agustina Dwi Wijayanti Ahmad Sofyan Akbar Agus Anshori Mussama Anastasia Endang Tri Hastuti Wahyuni Antasiswa Windraningtyas Rosetyadewi Arfian Rahma Shanti Arini Nurhandayani Aris Haryanto Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Claude Mona Airin Devi Yunita Sari Dewi Noor Hidayati Dini Wahyu Yudianingtyas Dito Anggoro Dito Anggoro Dito Anggoro Dyah Ayu Widiasih Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto Ferdinand Prayogo Cahyo Santoso Fidyah Fitrawati Fransiskus Trisakti Haryadi Hendra Herdian Heru Susetya I Nyoman Suarsana I Wayan Suardana I wayan Teguh Wibawan Ifah Khairunnizak Iwan Harjono Utama Khrisdiana Putri Khrisdiana Putri, Khrisdiana Koichi Akiyama Krisdiana Putri Lehgarubini Shanmuganathan Liza Angeliya Liza Angeliya Liza Angeliya Lusty Istiqomah Maria Anggita Marla Anggita Maxs Urias Ebenhaizar Sanam, Maxs Urias Medania Purwaningrum Mohammad Faiz Karimy Niken Respati Maharani Nur Khusni Hidayanto Nyoman Reishita Andriyani Okti Herawati Purnama Edy Santosa Radhian Fadiar Rahmat Setya Adji Ratna Ermawati Risang Aji Dewandaru Sarwo Edy Wibowo Sidna Artanto Sidna Artanto Sidna Artanto Sidna Artanto Sidna Artanto Sitarina Widyarini Siti Andarwarti Sugiyono Sugiyono Surya Amanu Surya Amanu Surya Amanu Surya Amanu Surya Amanu Surya Amanu Syarifah Alawiyah Tri Guntoro Tri Untari Tri Untari Tri Untari Tri Untari Ukon Susetyo Widagdo Sri Nugroho Widya Asmara Widya Asmara Widya Asmara Widya Asmara Widya Asmara Yuli Purwandari Kristiangingrum Yustina Yuni Suranindiyah