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Sigit Setyawan
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Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Medicine & Pharmacology Public Health Other

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Journal : Nexus Kedokteran Translasional

 1 
The Nefroprotector Effect of Salam Leaf Extract (Syzygium polyanthum) in Mices Renal Cell which is Induced by Paracetamol Asaduddien Faras; Endang Listyaningsih S; Sigit Setyawan
Nexus Kedokteran Translasional Vol 2, No 2 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background : Salam Leaf (Syzygium polyanthum) contains antioxidants such as flavonoids, selenium, vitamin A, vitamin C, vitamin E, triterpenes, tannins, saponins, niacin, and essential oils as an antioxidant compounds. In present study, Syzygium polyanthum leaves extract was evaluated for its protective effect on paracetamol- induced renal damage in mice. Methods: This study was laboratory experimental research with Randomized Controlled Trial design. A total of 28 Swiss Webster mices were equally divided into 4 groups. Mices for control group (KK) and the first group (KP1) will be given aquades for 14 day. The second(KP2) and third (KP3) group will be given Salam leaf extract with dose 7 mg/20 g and 14 mg/20 g weight of mice for 14 days. While paracetamol in toxic dose was given on to KP1, KP2, KP3, with dose 0,1 ml/ 20 g weight of mice on day 12, 13 and 14. Mice were sacrificed with neck dislocation on the 15th day and histological preparation was made to evaluate histological damage on proximal tubular renal cells stained by Hematoxillin Eosin (HE). Renal histological features were assesed by counting the number of proximal tubular renal cells histogical damage. Data were analyzed by One-Way ANOVA test (? = 0,05) continued by Post Hoc Multiple Comparisons test (LSD)(? = 0,05). Results: The results of One-Way ANOVA test showed p < 0.05 that there was significant difference between four-groups. LSD test results showed p < 0,05 there was significant difference between grups. Conclusion: Salam leaf extract can prevent the renal cell damage of mice. The difference dose of salam leaf extract give different effects nefroprotector in mices renal cell. Keywords : salam leaf extract, paracetamol, renal histologic structure damage
Antibacterial Activity Test of Aloe vera L. Leaf Extracts on Extended Spectrum ?-Lactamase (ESBL) Producing Bacteria from Surgical Site Infection Isolate . Zakiatunnisa; . Maryani; Sigit Setyawan
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Surgical site infection is clinically defined as a discharge of purulent around wounds or cellulitis inside postoperative wounds. One of the factors that cause surgical site infections is the ESBL producing bacteria that developed a resistance towards most antibiotics. Aloe vera L. is known to possess a lot of antibacterials potent against various bacteria, either gram-positive or gram-negative. This research aims to prove the effects of the administration of Aloe vera L. leaf extracts towards the growth of ESBL producing bacteria from surgical site infection isolates. Method of research: This research was an experimental laboratory research (post test only with control group design) with sampling technique non-probability sampling ; consecutive sampling. The research was done in the UNS Faculty of Medicines Laboratory of Microbiology. Extraction of samples was done in the Dr. Moewardi Public Hospital Laboratory of Microbiology.The independent variable of this research was 70% ethanol extracts of Aloe vera L. leaf with concentrations of 25%, 50%, 75%, and 100%. The research subject was surgical site infection isolate ESBL producing bacteria, standardized with Mc Farland 0.5. Sensitivity test on the Muller-Hinton medium used the diffusion method with cefoperazon/sulbactam as positive control and aquades as negative control. The result of research was tested with Kruskal-Wallis testing method followed by Mann-Whitney testing method. Results: Kruskal-Wallis test results indicates a significant interception potential between two treatment groups (P = 0.000). Mann-Whitney test results indicates a significant difference of interception potentials on every treatment group, with the exception of the interception potential groups of 75% concentration extract and 100% concentration extract, no significant interception potential was found (P = 0.179). Conclusion: Aloe vera L. leaf extract proves to possess potential activity interception towards the growth of ESBL producing bacteria from surgical site infection isolates. The concentrations of Aloe vera L. leaf extract possess a positive correlation towards the interception potentials on surgical site infection ESBL producing bacteria. Keywords: Infection, postoperative wound, ESBL, Aloe vera.
The Effect of Mangosteen Peel (Garcinia mangostana L) Extract Against Mice (Mus musculus) Kidney Cell Damage Induced by Paracetamol Namira Qisthina; Bambang Widjokongko; Sigit Setyawan
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Mangosteen peel (Garcinia mangostana L) contains antioxidant such as xanthone core, ?-mangostin, ?-mangostin, ?- mangostin, and garcinone which is thought to be nefroprotector. This study is aimed to investigate the mangosteen peel (Garcinia mangostana L) extracts nefroprotector effect against mice (Mus musculus) kidney cell damage induced by Paracetamol. Methods: This is an experimental laboratory study with post test only controlled group design. Swiss Webster mice weighing around 20 g were selected as sample. Those mice were divided into 4 groups randomly, each group consist of 7 mice. Incidential sampling was used as a sampling technique. Mice in control group (KK) and first treatment group (KP1) were given aquades, while second treatment group (KP2) and third treatment group (KP3) were given 10 mg and 15 mg of mangosteen peel extract respectively. KP1, KP2, and KP3 were given 0.1 ml paracetamol on the 8th, 9th, and 10th day of the treatment. Mice were sacrificed on 4th day and the kidneys were made into preparate using paraffin block and HE staining method. Histological kidney cells were assesed by the sum of pyknosis, karyorrhexis, and karyolysis cells. Data were analyzed using One Way ANOVA test continued by Post Hoc Multiple Comparisons (LSD). Result: One-Way ANOVAs test result showed a significant differences between four groups. LSDs test result showed a significant differences between KK-KP1, KK-KP2, KK-KP3,KP1- KP2, KP1- KP3, KP2-KP3. Conclusion: Mangosteen peel extract can prevent mices kidney cell damage induced by parasetamol. Keywords: Mangosteen peel extract, parasetamol, mices kidney cell damage
The Effect of Mangosteen Rind Extract (Garcinia mangostana L) to the Damaging of Hepar Cell of Mice (Mus musculus) Induced by Paracetamol Winda A Panjaitan; S B Widjokongko; Sigit Setyawan
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: The antioxidant properties of mangosteen rind extract such as xanthones, vitamin B1, vitamin B2, and vitamin C are estimated can protect hepar from free radicals and reducing NAPQI which produced by paracetamol metabolism. The purpose of this research is to know the effect of mangosteen rind ethanol extract to the damaging of hepar cell of mice induced by paracetamol. Methods: This research was a laboratorial experiment with post test only control group design. Samples of 28 male mice, Swiss webster type, 2-3 months old age and 20 gram of each weight, were divided into 4 groups in random or 7 mice each group. Aquadest were administered to mice in Control Group (KK) and the First Treatment Group (KP1), while mangosteen rind extract were administered to the Second Treatment Group (KP2) and the Third Treatment Group (KP3) with the dose of 10 mg/20 g body weight of mice and 15 mg/20 g body weight of mice. Paracetamol were administered to KP1, KP2, KP3, with dose 5.07 mg/20 g body weight of mice. On day 4th, mice were sacrificed with neck dislocation. Hepar cell slides were made and stained by HE. The hepar cell damage of mice were observed by counting number of necrosis cell. The data was analized by One-Way ANOVA test (? = 0.05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences (LSD) test (? = 0.05). Results: The data showed that average number of necrotic nucleus of KK was 9.71; KP1 was 72.86; KP2 was 14.00; and KP3 was 22.29. The results of One-Way ANOVA test showed a significant difference in all group (p < 0.05). The statistical data result showed a significant difference of necrotic nucleus between K-KP1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 (p < 0.05). Conclusion: The feeding of mangosteen rind was able to decrease hepar cell damage of mice which is induced by paracetamol. Keywords: mangosteen rind extract, paracetamol, the damaging of hepar cell
Co-Authors . Maryani . Zakiatunnisa Afifah Syifa Khairunnisa Agik Dwika Putra Akrom, Akrom Anggun Pulihana Wilujeng Apriliana Vivy Candra Ardhia Fefrine Indarta Asaduddien Faras Aulia Farah Diba Auliya Salsabilla Bambang Widjokongko Dimas Agung Pramudikto Dwi Budi Narityastuti Endang Listyaningsih S Endang Syahrir Firstiafina Tiffany Hanne Dianta Pramono Ilham Satria Raditya Putra Lilik Wijayanti Namira Qisthina Ony Hardoko S B Widjokongko Satya Nugroho Senyum Indrakila Siti Marufah Sri Haryati Sugeng Purnomo Teguh Ariyanto Victoria Husadani Permata Sari Viola Belivia Tripuspita Widia Susanti Winda A Panjaitan Yulia Lanti Retno Dewi Yulia Sari Yusuf Arif Salam