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Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D) Azharia Khalida; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 7, No 2 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.2.109-117.2019

Abstract

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.
Efektivitas Media Tanam Untuk Perbanyakan Spora Glomus Hasil Isolasi dari Rizosfer Pternandra echinata Jack. Dezi Meutya Dapersal; - Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 3, No 2 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.2.%p.2014

Abstract

The study about effectivity of growth medium for multiplication Glomus spores isolated from the rhizosphere Pternandra echinata Jack. has been conducted from February to November 2013 in the greenhouse and Plant Physiology Research Laboratory Department of Biology, Faculty of Mathematic and Natural Science Andalas University. The aims of the study to determine the appropriate type of media for multiplication Glomus isolated from rhizosphere Ptenandra echinata. The study used a Completely Randomized Design with 3 treatments and 9 replications. The treatment consists of the type of media used are sand, zeolite, and zeolite sand. The results showed that statistically media used gives a significantly different effect on the increase in plant height and number of leaves. Meanwhile the spore density and dry weight of the spores will show results that are not significantly different. Percentage of degree infection maize in all growth medium showed high criteria. Generally, the growth medium sand, zeolite, zeolite sand effective for spore multiplication glomus isolated from Pternandra echinata Jack.Keywords : Effectivity, Glomus, Growth Medium
Respon Pertumbuhan Nodus Artemisia vulgaris L pada Medium Murashige-Skoog dengan Penambahan Beberapa Zat Pengatur Tumbuh Secara In Vitro Novia Rika Deli; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 3 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.3.%p.2015

Abstract

Artemesia vulgaris L produce artemisinin which is effective against resistant strains of Plasmodium falciparum, the malarial parasite. The study about growth responses of Artemesia vulgaris L in Murashige-Skoog (MS) medium supplemented with several growth regulators had been done from September to November 2014 at the Laboratory of Plant Phisiology and Tissue Culture, Department of Biology, Faculty of Mathematic and Natural Science, Andalas University. The aims of the study was to know the responses of node explants of Artemesia vulgaris L supplemented with several plant growth regulators for the effective technique of propagation. The study used Completely Randomized Design with four treatments and six replications. Node from in vitro growth were treated with three different kind of growth regulator (0.3 ppm BAP, 0.5 ppm NAA, 0.3 ppm GA3) in MS medium. The result showed that 0.5 ppm NAA was the best growth regulators formed callus, bud, and root of Artemesia vulgaris L.Keywords: Artemesia vulgaris L, plant growths regulator, in vitro
The Growth of Coastal Cottonwood (Hibiscus tiliaceus Linn.) Seedlings by Inoculating Arbuscular Mychorrhiza Fungi (AMF) on Sand Beach Planting Media Nailul Rahmi Aulya; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 8, No 2 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.2.36-40.2020

Abstract

Hibiscus tiliaceus L. is a seaside plant that has several benefits, one of which is as a producer of paper fiber. H. tiliaceus need a better nutrient to grow on coastal land that is still constrained by the high level of salinity. By this, a special treatment is needed to support its growth. One of them is the application of mycorrhizal inoculants. Mycorrhizae is known as one of the fungi that can help plants tolerate salinity. The aims of the research were to determine the effect of AMF inoculants and determine the best doses of AMF to the seedling growth. The research used completely randomized design with 5 treatments and 5 replications. The treatments were (A) without AMF (control), (B) inoculation AMF 10 g/plant, (C) inoculation AMF 15 g/plant, (D) inoculation AMF 20 g/plant and (E) inoculation AMF 25 g/plant. The result showed that AMF can infect the roots of hibiscus seedlings with an infection degree of 18-34%. However, it has not been able to have a significant effect on shoot length, number of leaves, dry weight of root, and dry weight of shoot.
Pengaruh Penggunaan Fungisida (Dithane M-45) Terhadap Pertumbuhan Tanaman Jagung (Zea mays L.) dan Kepadatan Spora Fungi Mikoriza Arbuskula (FMA) Endah Mutia Sari; - Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

The research about effect of fungicide (Dithane M-45) on growth of corn (Zea mays L.) and spore density of arbuscular mycorrhizal fungi (AMF) had been done from August to November 2013 in a wire house at Laboratory of Plant Physiology, Departement of Biology, Faculty of Sciences, Andalas University. The research used Completely Randomized Design (CRD) with 7 treatmens and 4 replications. The treatments were varions doses of Dithane M-45; A (0 g/l), B (1,5 g/l), C (2,0 g/l), D (2,5 g/l), E (3,0 g/l), F (3,5 g/l), G (4,0 g/l). The results showed that Dithane M-45 decreased height and number of leaves of corn plants, leaf dry weight and spore density of AMF. The higher doses of fungicide used the fewer spores were obtained.Keywords: mycorrhizal, fungicide, Zea mays
Pengaruh Konsentrasi Giberelin (GA3) terhadap Pertumbuhan Kailan (Brassica oleracea L. Var alboglabra) pada Berbagai Media Tanam dengan Hidroponik Wick System Asih Maharani; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 6, No 2 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.2.63-70.2018

Abstract

Effect of giberelin (GA3) concentration on growth and yield of Chinese kale (Brassica oleracea L. Var alboglabra) in various medium using hydroponic wick system . This research conducted from April until July 2016 in Wire House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University. The purposes of this research were to obtain GA3 concentration and planting medium that accelerate growth and yield of Chinese kale and determine the interaction between GA3 and planting medium to the gowth and yield of Chinese kale planted using hydroponics wick system. This research used Completely Randomized Design (CRD) in factorial with three replications, as the first factor were concentration of GA3 (0 ppm, 20 ppm, 40 ppm and 60 ppm) and the second factor were medium (zeolite, sand, husk and sawdust). The best result in accelerated the growth of Chinese kale was the treatment at the concentration of 60 ppm GA3 and zeolite was suitable medium to Chinese kale growth. This research found the interaction between GA3 and medium on the length of the roots Chinese kale used hydroponic wick system.
Pertumbuhan Bibit Hopea mengarawan Miq. Pada Intensitas Cahaya Berbeda Elfans Bawalsyah S.A.; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 4, No 2 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.2.%p.2015

Abstract

An experimental study on growth of Hopea mengarawan Miq. seedlings under different light intensity was conducted from June to October 2012 at open field. This experiment aimed to find out optimal light intensity for the growth of H. mengarawan seedlings. The study used completely randomized design with four treatments and six replications. The treatments were control (100), 80, 60 and 40% of light intensities. The result showed  light intensity did not give significant effect to the growth of H. mengarawan seedlings.
Induksi Tunas Kunyit Putih (Curcuma zedoaria Roscoe) Pada Media MS Dengan Penambahan Berbagai Konsentrasi BAP dan Sukrosa Secara In Vitro Dola Ratna Yulizar; Zozy Aneloi Noli; M Idris
Jurnal Biologi Universitas Andalas Vol 3, No 4 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.4.%p.2014

Abstract

Murashige-Skoog media enriched using 6-Benzyl Aminopurine (BAP) and sucrose was conducted from June 2013 to February 2014 at the Laboratory of Plant Physiology, Department of Biology, Andalas University. This study aimed to determine the ability of shoot formation of white turmeric (Curcuma zedaoria Roscoe) on the effect of BAP and sucrose concentration. We used a Completely Randomized Design (CRD) with 8 treatments and 3 replications, BAP (0, 1.5, 3 and 4.5 mg/L) and sucrose (3 and 5%) of MS medium. The result showed that the percentage of shoot induction was 100%. The best combination of sucrose and BAP in shoot induction was obtained on 5% sucrose and 1.5 mg/L BAP. While for the first immerge of shoot was obtained on 5% sucrose and 4.5 mg/L BAP in 5 days after inoculation. All treatments but did not give any significant effect to the number and height of shoots.Key words: Curcuma zedoaria, shoot, sucrose and 6-Benzyl Aminopurine
Pengaruh Pupuk N dan Cekaman Kekeringan terhadap Pertumbuhan dan Kandungan Artemisinin Tanaman Artemisia vulgaris L. Elvina Sari; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 6, No 2 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.2.71-78.2018

Abstract

The research about effect of Nitrogen fertilizer and stress water on the growth and artemisinin content of Artemisia vulgaris L. has been conducted from December 2015 to March 2016 in Screen House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University, Padang. The purpose of this research were to the effect of  Nitrogen fertilizer, stress water, and their interaction between on the growth and artemisinin content of A. vulgaris. This research used Completely Randomized Design (CRD) in factorial with  four treatments ad three replications. The result showed that the number of leaves, plant height, length of roots, fresh weight, dry weight and of Artemisia vulgaris L. were increased significantly on N fertilizer treatments. The treatment of stress water 60% of volume capacity gave significantly effect on numbers of leaves and the highest content of Artemisinin was 80% volume capacity. The interaction of  N fertilizer and stress water did not showed significantly effect on the growth and artemisinin content of Artemisia vulgaris L.
Aplikasi Pupuk Organik Cair Menggunakan Bioaktivator Mikroorganisme Indigenous HPPB Untuk Pertumbuhan Desmodium heterophyllum pada Tanah Bekas Tambang Batu Kapur PT. Semen Padang Rizqa Zidna Chairafahmi; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 6, No 1 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.1.6-14.2018

Abstract

The research about application of liquid organic fertilizers with indigenous microorganism bioactivator of HPPB for growth of Desmodium heterophyllum on limestone mined land at PT. Semen Padang, had been conducted from May to August 2015 in Nursery and Reforestation, Laboratory of Plant Physiology Department of Biology, and the Laboratory of Soil Science Faculty of Agriculture, Andalas University, Padang. The research aimed to determine the effect and concentration of Liquid Organic Fertilizers (LOF) by using indigenous microorganism bioactivator originated from HPPB in limestone mined land ofPT. Semen Padang. This study used Completely Randomized Design (CRD) with five treatments and four replications. The treatments were control without LOF (A), 10% LOF (B), 20% LOF (C), 30% LOF (D) and 40% LOF (E). The results showed that 10% LOF was the best concentration to increase the number of leaves (36,44), percentage of land cover (6,71%) and fresh weight of plants (5,15g).