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Journal : Jurnal Biologi Universitas Andalas

Shoot induction on several cutting types of corm of shrimp banana (Musa acuminata Colla) through in vitro culture Mayta Novaliza Isda; Elvianis Elvianis; Siti Fatonah
Jurnal Biologi Universitas Andalas Vol 8, No 1 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.1.20-28.2020

Abstract

Musa acuminata Colla is a unique genomic species which differs from other species in this genus. It has typical color of reddish-purple on its fruit. Musa acuminata is recorded as rare banana in Riau, particularly in Kampar District. This research aims to study the influence of several cutting types of corm of shrimp banana originated from Kampar District through in vitro culture. It also purposes to determine the best formation of M. acuminata shoot through various concentration treatments of BAP only and BAP combined with Kinetin. The study used randomized block design (RBD) by adding come BAP concentration (0, 4, 8 mg/l) and BAP combined with Kinetin (0 mg/l BAP+0.4 mg/l Kinetin, 4 mg/l BAP+0.4 mg/l Kinetin, and 8 mg/l BAP+ 0.4 mg/l Kinetin). The cutting type of banana corm involve no cut (whole) and half-to-half cutting in MS media, each with 5 replicates. The result showed that either addition of BAP and BAP combined with Kinetin gave the best percentage of live explants and shot formation up to 100%. Treatment of 8 mg/l BAP resulted in the highest shoot percentage up to 100%, the fastest shoots appearance at 34.00 days after planting and the longest shoot reached 2.83 cm with 2.33 shoots counted on half-to-half cutting type. This study also confirmed that the addition of plant regulation substances from cytokinin group gave good impact in the formation of shrimp banana shoots.
Paku Kawat Lycopodiella cernua (L.) Pic. Serm. (Lycopdiaceae-Lycopodiales) dari Provinsi Riau – Kajian Morfologi dan Sekuen DNA berdasarkan Primer RBCL Nery Sofiyanti; Mayta Novaliza Isda
Jurnal Biologi Universitas Andalas Vol 7, No 1 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.1.43-50.2019

Abstract

Moss club (Lycopodiella cernua) is one of fern members distributed in Riau Province. The aimed of this study were to characterized the morphological characters and to analized its DNA sequence based on rbcl primer. Samples were collected from the field, documented and characterized the morphological charaters. DNA were isolated and amplified using PCR method and rbcl primer.  DNA sequnces were then analized using BLAST   Lycopodiella cernua collected from Riau Province was characterized by having creeping and erect rhizome that covered by microphyl, and terminal strobili. Rbcl fragmen of Lycopodiella cernua was sucssesfully amplified with 643 bp length. BLAST result confirmed the  rbcl fragmen of this club moss.
Root induction on siam orange (Citrus nobilis Lour.) originated from Kampar using in vitro shoot in MS media enriched with IBA and NAA Rasyidah Ulfa; Mayta Novaliza Isda
Jurnal Biologi Universitas Andalas Vol 8, No 1 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.1.29-35.2020

Abstract

Siam orange (Citrus nobilis Lour.) is a prominent agriculture commodity in Riau province. It tastes sweet, with fragrant aroma and thin skin. Its cultivation, however, needs improvement to gain better quality of product. One effort that is using the in vitro culture. The study aimed to determine the best IBA and NAA concentrations to trigger root formation on siam orange transplant and used Completely Randomized Design (CRD). The result showed that the rooting reached 100% on all treatments. The fastest rooting was observed in 2 mg/L IBA treatment (7.67 days after planting). The most root growth was obtained with combination of 0.5 mg/L NAA with 1.5 mg/L IBA (2.67 units). The longest root was obtained through the mixture of 1.5 mg/L NAA with 0.5 mg/L IBA (2.27 cm).
Addition of Benzyl Amino Purine (BAP) to Induction of Drendan Seeds Buds (Lansium domesticum var. aqueum (Jack) Miq.) by In Vitro Mayta Novaliza Isda; Febby Ika Desyana
Jurnal Biologi Universitas Andalas Vol 8, No 2 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.2.61-68.2020

Abstract

Drendan (Lansium domesticum var. aqueum (Jack) Miq. is one of Indonesia's native tropical fruits. Drendan has high antioxidant activity and contains high nutrients, but has started to rarely be found in the field because its management and cultivation is rarely done due to its sour taste in vitro culture is a method or technique of plant propagation which can be an alternative to obtaining drendan seedlings in large quantities and in a short time vegetatively. The purpose of this study was to determine the effect of BAP on shoot induction from drendan seed explants and determine the best concentration of BAP on shoot growth of drendan seed explants in vitro. This study used a completely randomized design with BAP concentrations of 0, 1, 3, 5 and 7 mg / L BAP. The result of this research is the addition of BAP has been able to increase the percentage of live explants and the percentage of shoot formation. Giving BAP did not significantly affect the number of shoots and had a significant effect on shoot emergence time and shoot length. The addition of BAP resulted in slower shoot emergence time. Giving BAP was not able to stimulate shoot induction, but the addition of BAP could increase shoot length growth at a concentration of 5 mg / L BAP of 0.52 cm.
In Vitro Response of Protocorm Grammatophyllum stapeliiflorum (Teijsm. & Binn.) J.J.Sm. Orchid in Growth on Several Media Composition Ranny Wirmasari; Mayta Novaliza Isda
Jurnal Biologi Universitas Andalas Vol 7, No 2 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.2.118-125.2019

Abstract

Family Orchidaceae has about 800 genera which are already difficult to find and almost extinct, including the orchid genus Grammatophyllum. A species of rare orchids, Grammatophyllum stapeliiflorum, is hard to find in original habitat. The addition of Growmore, BAP and 15% coconut water is expected to increase the growth and development of the G. stapeliiflorum orchid protocorms. This study use a Completely Randomized Design, consisting of 6 treatments (control, 1 and 3 mg/l BAP, 1 and 3 g/l Growmore, and 150 ml/l coconut water) in MS media with 5 replicates with observation for 4 weeks after planting. The results showed did not differ markedly in the number of protocorms, number of shoots and number of browning protocorms, but differ markedly in the parameter protocorm color based on DMRT test. Treatment of 3 mg/l BAP gives the best results on the number of shoots 2,60 protocorms and number of browning protocormss at the least amount 0,80 protocorms. The number of protocorms most widely on the treatment of 1 g/l Growmore 14,40 protocorms. Protocorms color is best found in 3 g/l Growmore with green color. This research managed to multiply the number of protocorms and induces protocorms shoots from Grammatophyllum stapeliiflorum orchid.
The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro Maya Sari; Mayta Novaliza Isda
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.8-17.2021

Abstract

The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting.
Co-Authors Adil Azzahwantara Adjie, Agung Purnomo Afragenia Zelfira Agung Purnomo Adjie Alzira Salsabillah Anwar, Nurhayati Appriliya Destiyana Ariya Permata Asih Rahayu Ajeng Agesti Asri Ria Lestari Ayu Azhari Chica Ollivia Fibrianti Defila Yanti Desita Salbiah DESTI ZARLI MANDARI, DESTI ZARLI Dewi, Reza Puspita Dhini Yulianti Diana Agriani Diandra Prudentia Dyah Iriani EFENDI, RANNY ARIESTA Elli Indriana Putri Elsya Desviyanti Elvianis Elvianis Ennie Chahyadi ERWINA JULIANTARI, ERWINA Fajar Nugraha Febby Ika Desyana Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitria Elysye Ningrum Fransisca Elvarina Hariono, Eko Haris Gunawan Ikhwan Taufik Ilahi, Rizky Nanda Kurnia Ismu Sodaqti Isra Fariza KHAIRIJON, KHAIRIJON Lailani Sabrina Laura Aprilia Ayu Kusuma Wahyu Priyadika M. Athallah Dzikri Alhady M. Rizqy Pratama Syah Marlina Agustina Sitinjak Maulana Ishak Maya Sari Meutia Hanum Meyla Suhendra Mimi Safitri Muhammad Asyraf Muhammad Tamyis Lutfi Hakim Nadia Kristina Najmi Fadhila Nithami Nery Sofiyanti Nia Ivanka Noto Prasetio Nurhafidha Ramadhani Nurhayati Anwar Oriza Athalia Ronald Pangeran Saud Fransisco Putri Maisarah Qintara Sahira Rafi Shadiq Alvi Rahmi Fitri Rana, Syafia Diang Ranny Wirmasari Rasyidah Ulfa Reza Puspita Dewi Riche Afrilla Riski Saputra Rizal Noor Rodesia Mustika Roza Rosmaina Rosmaina Saputra, Agus Sarah Novita Sari Umayah, Sari Savanah Zahra Sinaga, Patar Siti Fatonah Siti Fatonah Siti Fatonah SRI CAHYATI, SRI Surjawati Surjawati Syafia Diang Rana syafroni Pranata Syahrul M. Tirtha Juliana Titrawani Titrawani Vina Dwi Amanda Wahyu Lestari Wahyu Lestari Wulan refolla Yana Lisa Warni Yulia Rahmi