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All Journal Biospecies Jurnal Biologi Udayana AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Biologi Universitas Andalas Jurnal Riau Biologia Jurnal Ilmiah Pertanian Jurnal Biodjati Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Bio-Lectura Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal KALANDRA
Mayta Novaliza Isda
Jurusan Biologi FMIPA Universitas Riau, Kampus Bina Widya Panam, Pekanbaru, Riau, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences

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Journal : Jurnal Ilmiah Pertanian

 1 
Pemberian air rebusan kentang pada media Murashige-Skoog terhadap pertumbuhan protokorm anggrek sendu (Grammatophyllum stapeliiflorum) secara in vitro Fitria Elysye Ningrum; Mayta Novaliza Isda
Jurnal Ilmiah Pertanian Vol. 19 No. 1 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i1.8514

Abstract

Grammatophyllum stapeliiflorum is one of the most popular orchid species because of its beauty and is characterized by the shape of the flower stalk that sticks down and the brown flowers with white and yellow spots that look like drops of water or sad. This type of orchid is almost extinct and is rarely found in its natural habitat, so it is necessary to perform in vitro propagation of orchids. The culture in vitro method is one of the most effective methods for propagating rare orchids. This study aimed to determine the effect of adding potato water on protocorm growth and determine the best concentration for the growth of G. stapeliiflorum protocorm in vitro. This study used a Completely Randomized Design (CRD) with the addition of potato water (concentrations 0, 50, 100, 150, and 200 ml/L) on Murashige-Skoog (MS) medium. The results showed that the percentage of live explants was 100% in all treatments. The addition of potato water to MS medium had no effect on the percentage of live explants but still affected the growth percentage of Protocorm-like bodies (PLBs) in vitro. The best concentration of potato water for the protocorm growth of G. stapeliiflorum in vitro to MS medium was at a concentration of 150 ml/L, which was indicated by the highest percentage of PLBs growth at 100% and the color of PLBs green (2.5GY 6/8) compared to other potato water treatments and controls.
In vitro shoot induction from petiole explants of tembesu (Fagraea fragrans Roxb) using 6-benzylaminopurine Mayta Novaliza Isda; Riche Afrilla
Jurnal Ilmiah Pertanian Vol. 20 No. 3 (2023): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v20i3.11772

Abstract

Tembesu (Fagraea fragrans Roxb) is a valuable woody plant from the Loganiaceae family, utilized in construction and furniture production. Unfortunately, the tembesu population continues to decline due to excessive logging, forest fires, and a lack of cultivation efforts. Planting through seeds takes a long time, making in vitro propagation an attractive alternative. This research aims to evaluate the effect of 6-benzylaminopurine (BAP) in inducing shoots and determine the optimal BAP concentration on tembesu petiole explants in vitro. A completely randomized design (CRD) with six treatment levels (0, 0.5, 1, 1.5, 2, and 2.5 mg/L BAP) and five replications was employed. Despite a 10% shoot formation rate for each treatment, the optimal BAP concentration for callus formation was found to be 0.5 mg/L, with the highest percentage reaching 70%. The results indicate that adding different concentrations of BAP to the Murashige & Skoog (MS) medium was ineffective in inducing shoots in tembesu petiole explants. Therefore, further research and the development of more sophisticated in vitro propagation techniques are necessary to enhance the success of shoot induction in tembesu. It is hoped that this research will serve as a foundation and motivation for subsequent in-depth studies on the in vitro propagation of tembesu, supporting the conservation and development of this plant.
Co-Authors Adil Azzahwantara Adjie, Agung Purnomo Afragenia Zelfira Agung Purnomo Adjie Alzira Salsabillah Anwar, Nurhayati Appriliya Destiyana Ariya Permata Asih Rahayu Ajeng Agesti Asri Ria Lestari Ayu Azhari Chica Ollivia Fibrianti Defila Yanti Desita Salbiah DESTI ZARLI MANDARI, DESTI ZARLI Dewi, Reza Puspita Dhini Yulianti Diana Agriani Diandra Prudentia Dyah Iriani EFENDI, RANNY ARIESTA Elli Indriana Putri Elsya Desviyanti Elvianis Elvianis Ennie Chahyadi ERWINA JULIANTARI, ERWINA Fajar Nugraha Febby Ika Desyana Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitria Elysye Ningrum Fransisca Elvarina Hariono, Eko Haris Gunawan Ikhwan Taufik Ilahi, Rizky Nanda Kurnia Ismu Sodaqti Isra Fariza KHAIRIJON, KHAIRIJON Lailani Sabrina Laura Aprilia Ayu Kusuma Wahyu Priyadika M. Athallah Dzikri Alhady M. Rizqy Pratama Syah Marlina Agustina Sitinjak Maulana Ishak Maya Sari Meutia Hanum Meyla Suhendra Mimi Safitri Muhammad Asyraf Muhammad Tamyis Lutfi Hakim Nadia Kristina Najmi Fadhila Nithami Nery Sofiyanti Nia Ivanka Noto Prasetio Nurhafidha Ramadhani Nurhayati Anwar Oriza Athalia Ronald Pangeran Saud Fransisco Putri Maisarah Qintara Sahira Rafi Shadiq Alvi Rahmi Fitri Rana, Syafia Diang Ranny Wirmasari Rasyidah Ulfa Reza Puspita Dewi Riche Afrilla Riski Saputra Rizal Noor Rodesia Mustika Roza Rosmaina Rosmaina Saputra, Agus Sarah Novita Sari Umayah, Sari Savanah Zahra Sinaga, Patar Siti Fatonah Siti Fatonah Siti Fatonah SRI CAHYATI, SRI Surjawati Surjawati Syafia Diang Rana syafroni Pranata Syahrul M. Tirtha Juliana Titrawani Titrawani Vina Dwi Amanda Wahyu Lestari Wahyu Lestari Wulan refolla Yana Lisa Warni Yulia Rahmi