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Journal : Jurnal Veteriner

Respons Hematologi dan Kimia Darah Domba Lokal Indonesia Terhadap Stres Transportasi Selama 12 Jam (HAEMATOLOGICAL AND BLOOD BIOCHEMICAL RESPONSES TO 12 HOUR TRANSPORTATION STRESS IN IN LOCAL INDONESIAN SHEEP) Sarmin Sarmin; Amelia Hana; Pudji Astuti; Yuda Heru Febrianto; Claude Mona Airin
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.246 KB) | DOI: 10.19087/jveteriner.2019.20.1.48

Abstract

The objective of this study was to investigate the effect of transport 12 hours on haematological and some biochemical responses of local Indonesian sheeps. Ten adults (2.0-2.5 years) male local Indonesian sheeps BW adult 30-40 kg were transported by L-300 pickup truck starting at 3:00 pm to 3:00 am on the d 2 without stopping. Jugular blood were collecte-d at 7 am (-8 h pre- transportation as baseline), 15:00 immadiately after (0 h), 19:00 (4 h), 23:00 (8 h) , 03.00 (12 h ), and at 11.00 d 2 (+8 h) post- transportation as recovery period). Under the conditions of the present study, transport of local Indonesian sheeps significantly increased glucose during 4 h transport (P <0.05), increased eosinophil at 0 h (P <0.05) and no significant changed in erythrocytes, haemoglobin , monocytes, neutrophils, lymphocytes, leukocytes, packed cell volume (PCV), and total plasma protein (TPP) (P> 0.05). Our findings showed that transportation 12 h affected eosinophil during 0 h and glucose metabolism during 4 h of transportation, but did not any affect on some haematology and dehydration responses in local Indonesian sheeps.
Diet Rendah Protein Meningkatkan Jumlah Neuron Nitrergik Duodenum dan Jejunum Serta Menurunkan Bobot Badan Tikus Wistar (LOW PROTEIN DIET INCREASES THE NUMBER OF DUODENUM, JEJUNUM NITRERGIC NEURONS AND REDUCES BODY WEIGHT IN THE WISTAR RATS) Raden Rara Amelia Hana; Sarmin Sarmin; Claude Mona Airin; Pudji Astuti
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.663 KB) | DOI: 10.19087/jveteriner.2018.19.4.539

Abstract

Protein is very important for the growth, development and activity in rats. The impact of lack protein intake to gastrointestinal, especially in the small intestine, the number of nitrergic neurons, and changes in body weight in Wistar rats has not been studied. Therefore the aims of this study to examine the effect of a low-protein diet for 4 weeks on the number of duodenal, jejunal, ilealnitrergicneuron and changes in body weight in Wistar rats. Nine male Wistar (Rattusnorvegicus) rats, aged 1 month, with an average body weight of 120 grams used in this study. The rats were adapted for 7 days in individual cages with a normal diet (containing 24% protein) and drinking ad libitum. After being adapted all rats were divided into 3 groups (K-24, K-14, and K-4), consist of 3 rats. Then all rats fasted for 12-hours. Each group received treatment as follows: K-24 group (as a control) was given a diet containing 24% of protein, K-14 group was given a diet containing 14% of protein, and K-4 group was given a diet containing 4% of protein . Diet and drinking water are given ad libitum. Along of treatment for 4 weeks.body weights and blood glucose were measured every weeks, then all rats were euthanized and sample tissues of duodenum, jejunum, and ileum were then were obtained for histopathological preparations using the NADPH-d technique. Data on the number of nitrergicmyenteric neurons in the duodenum, jejunum, and ileum, body weight gain, and blood glucose levels were statistically analyzed by single factor design Anova. The results of this study concluded that a low protein diet (14% and 4% protein) for 4-weeks in the duodenum, jejunum have been increased the number of nitrergic neurons, on the other hand, in the ileum have been decreased the number of nitrergic neurons, reduced body weight, and did not increase blood glucose levels in Wistar rats.
Profil Total Bilirubin, Aktivitas Alanine Transaminase dan Total Protein Domba Garut pada Umur dan Status Fisiologis yang Berbeda Sarmin Sarmin; Pudji Astuti; Claude Mona Airin; Nur Adianto
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.432

Abstract

The purpose of this study was to examine the profiles of total bilirubin, ALT activity, and total protein in Garut sheep at different ages and physiological stasus. A total of 36 head of Garut sheep with various physiological conditions (ewe, ram, female suckling lamb, male suckling lamb, female postweaning lamb, male postweaning lamb, young ram aged six month, young ram aged one, pregnant ewe, and lactating ewe) was used in this research. Blood samples were taken through the jugular vein in the morning before the sheep were fed. Then, the values of total bilirubin, alanine aminotransferase (ALT), and total protein were analyzed. The mean total bilirubin value of Garut sheep was 0.59 ± 0.49 mg/dL, influenced by age and not affected by sex or physiological status. The total bilirubin value of ewe lambs was the highest (1.85 ± 0.87 mg/dL) compared to the total bilirubin value at other ages. The average value of ALT activity in garut sheep was 18.84 ± 6.56 U/L, which is influenced by age but not affected by physiological status or gender. The value of ALT activity in female suckling lamb was the lowest (7.03 ± 0.81 U/L) compared to ALT activity at other ages. The average total protein of Garut sheep was 6.48 ± 0.83 (g/dL), which was affected by age but not affected by sex or physiological status. The lowest total protein was found in female suckling lamb (.51 ± 0.59 g/dL) compared to total protein values at other ages. It was concluded that age affected the values of total bilirubin, ALT activity, and total protein in Garut sheep. Therefore, the age of Garut sheep should be considered in the interpretation of total bilirubin, ALT activity, and total protein.