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All Journal Jurnal Biologi Udayana ILMU KELAUTAN: Indonesian Journal of Marine Sciences Biotropika Current Biochemistry Journal of Innovation and Applied Technology The Journal of Pure and Applied Chemistry Research Jurnal Kelautan Tropis Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Indonesian Journal of Chemistry Jurnal Perikanan Universitas Gadjah Mada Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Musamus Fisheries and Marine Journal Tomini Journal of Aquatic Science Bioscientist : Jurnal Ilmiah Biologi Nekton ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua
Dailami, Muhammad
Aquaculture Study Program, Faculty Of Fisheries And Marine Science, Brawijaya University, Veteran Street, Malang 65145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Journal : The Journal of Pure and Applied Chemistry Research

 1 
Analysis and Prediction of some Histone-derived Antimicrobial Peptides from Toads Duttaphrynus melanostictus and Phyrinoidis asper Muhammad Dailami; I Made Artika; Mirza Dikari Kusrini
The Journal of Pure and Applied Chemistry Research Vol 5, No 2 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1579.558 KB) | DOI: 10.21776/ub.jpacr.2016.005.02.231

Abstract

Antimicrobial peptides in skin secretions of toads is a promising methods to combat a wide spectrum of bacteria. Histone H2A is a type of DNA-binding protein that acts as a precursor for several antimicrobial peptides. In toads (family Bufonidae) buforin I and buforin II are examples of antimicrobial peptides that derived from histone H2A. This study investigated the genetic diversity and phylogenetic analysis and in silico prediction of antimicrobial peptides derived from histone H2A of Duttaphrynus melanostictus and Phyrinoidis asper, which were collected from Bogor Agricultural University’s campus area. A new set of primers (Buf_fwd and Buf_rev) were designed by using PrimerBLAST, to amplify 393 nucleotides of the histone H2A gene that codes 131 amino acids. Haplotype diversity of both species are very low. Phylogenetic analysis shows the sample D. melanostictus and P. asper are separated to each other in two different clades. Several short predicted peptides from histone H2A show a potential as an antimicrobial peptides based on in silico prediction. Psychochemical characteristics and 3D structure of potent antimicrobial peptides are described.
Co-Authors Abdul Hamid A. Toha Abraham Welliam Manumpil Agus Mohammad Hikam Aji Wahyu Anggoro Ambariyanto Ambariyanto Andrianus Sembiring Aradea Bujana Kusuma Asus Maizar Suryanto H Aulia Rahmawati Buana Insan Kamil Christopher P. Meyer Condro Puspo Nugroho Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Dandi Saleky Darma Santi Dean Rama Prayoga Delvira Riska Ananta Dewa Gede Raka Wiadnya Diah Permata Wijayanti Dodi Safari Dyah Kinasih Wuragil EDY H.P. MELMAMBESSY Eka Maya Kurniasih Elsa Syafi’ul Umma Febriyani Eka Supriatin Febriyani Eka Supriyatin Fitri Nur Azizah Hardoko Hardoko Hardoko Hardoko Hariati, Anik Martinah Heder Djamaludin Hendrikus R Siga Hermawaty Abubakar I MADE ARTIKA Indria Graha Hayuningtyas Isma Isma Jemmy Manan Lalu Panji Imam Agamawan Maftuch Maftuch Marius Agustinus Welliken Mirza Dikari Kusrini Mohammad Sholeh Uluwwi Mudjirahayu Mudjirahayu Muhammad Fakhri Muhammad Musa Murtihapsari, Murtihapsari Nasrullah Bai Arifin Ni Kadek Dita Cahyani Ni Putu Dian Pertiwi Novaldo Yuri Muhammad Nurul Jadid Mubarokati Putri Nur Azizah Reny Sianturi Roni Bawole Rosa D. Pangaribuan Simon P.O Leatemia Sri Andayani Supriatin, Febriyani Eka Surya Dewa Ramadhan Thomas F. Pattiasina Tresia S Tururaja Tristany Brighton Jorghi Vivi Nurhadianty Vivi Nurhadianty, Vivi Yenny Risjani Yuni Widyawati Yuni Widyawati Yusup A Jentewo Zeth Parinding