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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Medical Journal of Indonesia Jurnal Ilmu Ternak dan Veteriner

Hapsari Mahatmi

Laboratorium Bakteriologi Dan Mikologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234

Author-ID : 312093

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Perasan Daun Mengkudu (Morinda Citrifolia) Menghambat Pertumbuhan Bakteri Escherichia Coli Secara In Vitro KAMESWARI, MADE SUMITHA; MAHATMI, HAPSARI; KERTA BESUNG, I NENGAH
Indonesia Medicus Veterinus Vol 2 (3) 2013
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.355 KB)

Daun mengkudu (Morinda citrifolia) mengandung senyawa kimia seperti : antrakuinon, alkaloid, saponin, flavanoid, dan terpenoid yang berperan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kemampuan daun mengkudu (M. citrifolia) dalam menghambat pertumbuhan bakteri Escherihia coli (E. coli) secara in vitro. Penelitian ini menggunakan isolat bakteri E. coli ATCC 25922 yang diperoleh dari Laboratorium Mikrobiologi Fakultas Kedokteran, Universitas Udayana. Kemampuan perasan daun mengkudu untuk menghambat pertumbuhan bakteri E. coli ATCC 25922 diuji dengan uji hambatan metode Kirby-Bauer yang dimodifikasi. Rancangan penelitian yang digunakan adalah rancangan RAL (Rancangan Acak Lengkap) dengan lima perlakuan, satu kontrol positif dan empat kali ulangan. Hasil perasan daun mengkudu berasal dari 300 gram daun mengkudu yang dihaluskan kemudian diperas. Konsentrasi perasan daun mengkudu yang digunakan adalah 0%, 25%, 50%, 75%, 100% dan kontrol positif oxitetrasiklin. Semua data dianalisis secara statistik dengan SPSS 13 (Sampurna, 2008). Hasil penelitian menunjukkan bahwa perasan daun mengkudu secara signifikan mampu menghambat pertumbuhan bakteri E. coli (P<0,01). Rataan zona hambat yang terbentuk pada konsentrasi 0%, 25%, 50%, 75%, 100% secara berurutan adalah 0,00 mm 7,3 mm, 8,5 mm, 10,4 mm, 12,5 mm dan secara statistik sangat berbeda nyata. Ada kecenderungan semakin tinggi konsentrasi perasan daun mengkudu maka zona hambat yang terbentuk semakin besar dengan uji regresi yang di dapat Y = 0.091 + 0.411K - 0.006K2 + 0.00003320K3 dan r = 0,995.

Kontaminasi Salmonella spp pada Daging Ayam Broiler yang dijual di beberapa Pasar Tradisional di Makassar Darmawan, Alpian; Muslimin, Lucia; Arifah, Sitti; Mahatmi, Hapsari
Indonesia Medicus Veterinus Vol 9 (2) 2020
Publisher : Faculty of Veterinary Medicine, Udayana University

Daging ayam merupakan bahan komoditi asal hewan yang pada beberapa tempat masih diperdagangkan dengan sistem konvensional, sesuai dengan kebiasaan masyarakat setempat. Kandungan protein dan kandungan air yang tinggi pada daging ayam merupakan media pertumbuhan bagi berbagai bakteri yang bersifat non patogen maupun yang patogen. Peraturan pemerintah menyatakan bahwa Salmonella spp merupakan salah satu bakteri patogen yang tidak boleh ada dalam daging ayam ataupun produk hewan lainnya. Penelitian ini bertujuan untuk mengetahui kemungkinan adanya kontaminasi Salmonella spp pada daging ayam yang dipasarkan di enam pasar tradisional di wilayah Makassar. Sampel yang diperiksa sebanyak 24 sampel berupa daging ayam bagian dada yang diambil dari enam lokasi pasar yang berbeda yaitu Pasar Daya (Kecamatan Biringkanaya), Pasar Antang (Kecamatan Manggala), Pasar Terong (Kecamatan Bontoala), Pasar Pabbaengbaeng (Kecamatan Tamalate), Pasar Sambung Jawa (Kecamatan Mariso), dan Pasar Bacan (Kecamatan Wajo) Kota Makassar. Isolasi dan identifikasi dilakukan dengan pemeriksaan mikroskopis dengan pewarnaan Gram dan dilanjutkan dengan isolasi pada media Bistmut Sulfit Agar (BSA), Salmonella Shigella Aga (SSA), uji TSIA (Triple Sugar Iron Agar), IMViC (Indole, Methyl Red, Voges Proskauer, Citrat) dan uji urease. Hasil penelitian ditemukan bahwa 3 (12,5%) isolat dari 24 sampel yang diisolasi dan diidentifikasi, positif bakteri Salmonella spp. Kondisi ini memerlukan perhatian serius untuk segera mendapatkan tindak lanjut oleh pemegang kebijakan terkait sebagai upaya pencegahan penyebaran penyakit zoonosis.

Bakteri Escherichia coli Teridentifikasi pada Rektum Lumba-Lumba Hidung Botol Indo-Pasifik di Umah Lumba Rehabilitation Center, Taman Nasional Bali Barat Kristiawan, Vicky; Mahatmi, Hapsari; Sudipa, Putu Henrywaesa; Rahmadani, Deny
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.234

Saluran cerna merupakan salah satu tempat pertumbuhan berbagai genus serta spesies bakteri termasuk bakteri family Enterobacteriaceae. Salah satu dari anggotanya adalah Eschericia coli yang merupakan flora normal yang sering ditemukan pada saluran cerna. Saat ini masih sangat langka penelitian tentang keberadaan E. coli pada saluran cerna lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus). Tujuan penelitian ini adalah untuk mendapatkan data awal mengenai bakteri Enterobateriaceae pada lumba-lumba tersebut yang berada di lingkungan rehabilitasi. Sampel lumba-lumba yang digunakann merupakan lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di jaring apung laut atau seapen Umah Lumba Rehabititation Center, sebanyak tiga ekor. Pengambilan sampel dilakukan dengan menggunakan rubber catheter sterile berukuran 2,7 mm yang dimasukkan ke dalam rektum lumba-lumba. Jumlah total sampel yang di isolasi sebanyak 12 sampel dari tiga ekor lumba-lumba. Isolasi sampel dilakukan dengan menggunakan media Sheep Blood Agar, media Mac Conkey Agar dan Eosine Methylen Blue Agar. Selanjutnya di uji dengan pewarnaan Gram, uji katalase, uji oksidase, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, dan uji urease. Hasil dari penelitian ini dapat disimpulkan pada tiga ekor lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di seapen Umah Lumba Rehabilitation Center, ditemukan jenis bakteri famili Enterobacteriaceae spesies E. Coli.

Seroprevalence of Q fever in sheep and goat in West Java area Agus Setiyono; Ekowati Handharyani; Hapsari Mahatmi
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. Ruminant especially sheep and goats may play an important role in the transmission of the disease to human. The research of seroprevalence of Q fever in sheep and goats was carried out from August 2006 to March 2007 in West Java area. A total of 138 sera were collected; 69 sera from sheep and 69 sera from goats. The indirect immunoflourescent antibody test was used to determine the seroprevalence of Q fever. The seropositive based on the dilution of serum starting from 1 : 16. Seropositive were observed in 22 samples (31.88%) of sheep and 14 samples (20.28%) of goats. The highest titer of 1 : 128 was observed in 3 pregnant sheep. The results of the present study suggested that Q fever was endemic in West Java area. Key Words: Q Fever, Prevalence, Indirect Immunoflourescent Antibody Test

Resistensi Bakteri Aeromonas sp. Isolat Ikan Piranha (Pygosentrus nattereri) Bali Safari And Marine Park terhadap Antibiotik Ni Made Herawati; Hapsari Mahatmi; I Nengah Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.629 KB)

Piranha (Pygosentrus nattereri) is a fish that has been conserved at Conservation Center of Bali Safari and Marine Park. In May 2012 there was a case that twelve Piranhas suffered from illness and two of them died without any specific clinical signs. The dead Piranhas were necropsied and the tissues or parts of organs were collected for microbial examination interests. Spesies specific bacterial examination was also included using Microgen GNA TM + B-ID system test The bacterial examination found that the fishes were infected by Aeromonas hydropila and Aeromonas veronii. Further examination about antibiotic resistance tests showed that those Aeromonas spp were resistant to chloramphenicol and a combination of trimethoprim and sulphamethoxazole. It can be suggested that, next, the usage of other antibiotics is strongly recommended for the case of Piranha infected by Aeromonas spp at Bali Safari and Marine Park.

Pola Resistensi Salmonella typhi yang Diisolasi dari Ikan Serigala (Hoplias malabaricus) terhadap Antibiotik Waode Santa Monica; Hapsari Mahatmi; Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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The purpose of this study was to know the resistant pattern of Salmonella typhi (S. typhi) isolated from wolf fish (Hoplias malabaricus) to antibiotics in Bali Safari and Marine Park. S. typhi was isolated from fecal swab collected from the illness fish. The S. typhi was confirmed with MicrogenTM GN A ID test’s method. The antibiotic sensitivity of the S. typhi was tested using streptomycin, gentamicin, penicillin, vancomycin, and chloramphenicol according to the Kirby bouer method. The results showed that the isolate of S. typhi was resistant to penicillin and vancomycin, but sensitive to streptomycin, gentamicin, and chloramphenicol.

Detection of Mycobacterium bovis and Klebsiella pneumoniae at Bali Cattle Slaughterhouse by culture analysis and PCR I Gede Oka Darsana; I Nyoman Dibia; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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A study to determine the presence and prevalence of bovine tuberculosis and Klebsiella pneumoniae atcattle slaughterhouse in Bali was carried out in Pesanggaran and Mambal abattoirs from January to March2015.The Lungs and lymph nodes were inspected for lesions and then examined through DNA analysis forMycobacterium bovis and culture for isolation of Klebsiella pneumoniae. Of the samples examined, no onehad lesions suggestive of tuberculosis in two abattoirs.However, Klebsiella pneumoniae was observed1/4513 sample (0.02%) only in Mambal abattoir. This study is the first report of the presence of Klebsiellapneumoniae in Bali. Therefore, a aware of this zoonotic Klebsiellosis and its control managementformulation are need.

Deteksi Antibodi Mycobacterium tuberculosa bovis pada Sapi di Wilayah Kabupaten Buleleng, Bangli, dan Karangasem Provinsi Bali Putu Gede Widiarsa Putra; Nengah Kerta Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Bovine tuberculosis (BTB) is one the most widespread zoonotic diseases and has recently reemerged as a major health concern. The disease is caused by the Mycobacterium tuberculosis complex (MTBC) that primarily affects humans as its main host, and that is able to attack more hosts such as domestic animals, pet animals, and wild animals. This Cross-Sectional Study was carried out to determine the seroprevalence of BTB in Districts of Buleleng, Bangli, and Karangasem Province of Bali. One hundred and eighty cow blood samples were collected, and the serological status of BTB was detected using Elisa method. The results showed that the seroprevalence of BTB in District of Bangli was 2.22%, while, those was negative in Districts of Buleleng and Karangasem. The positive seroprevalence of BTB in Bangli indicates that the same research must be conducted throughout the districts in the Province of Bali.

Kepekaan Eschericia coli Patogen yang Diisolasi dari Babi Penderita Kolibasilosis terhadap Antibiotik di Kecamatan Kerambitan dan Tebanan Kabupaten Tabanan, Bali Aditya Nugraha; NK. Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.275 KB)

This study aims to determine the sensitivity pattern of E. coli isolated from pigs with colibacillosis to antibiotics. The total of 21 fecal samples was collected from pigs with white scour at the District of Kerambitan and Tabanan, the Regency of Tabanan, Bali. The isolation and identification of pathogenic E coli used Sheep Blood Agar medium, and serotype grouping of those bacteria was designated through agglutination test using serum’s antibody Polyvalent 1-5 and 6-11. The pathogenic E. coli found was then examined it`s own resistance to streptomycin, sulfamethoxazole, gentamicin, cefotaxime, and chloramphenicol according to Kirby Bouer method. The result showed that the isolates of E. coli polyvalent 1-5 were resistant to streptomycin and chloramphenicol, whereas that of polyvalent 6-11 was resistant to sulfamethoxazole, gentamicin, streptomycin and chloramphenicol.

Staphylococcus spp. pada Ikan Koi yang Dipelihara dalam Kolam Isolasi pada Masa Karantina Syafiana Fairizca; Hapsari Mahatmi; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Currently, koi fish enthusiasts and fans in various regions in Indonesia, especially in Bali, have increased very sharply. In the conditions of transportation from outside Bali to Bali, it triggers stress which causes the koi's resistance to decrease so that disease occurs. Therefore, a quarantine process is very necessary to get koi that are healthy and suitable for cultivation. Diseases in koi fish can be caused by viruses, parasites, and bacteria, one of which is Staphylococcus spp. Research on bacterial infections in koi fish is still very rare, especially regarding Staphylococcus spp. in koi fish in Indonesia and in Bali. This study was conducted to obtain preliminary data regarding Staphylococcus sp infection in koi fish. In this study, the object used was koi fish which has any symptomps of diseases or died during a 14-day quarantine period.The researched koi fish came from suppliers in Tulungagung and Blitar, East Java. The size of the koi fish ranged from 15 -25 cm which was kept in fish isolation ponds at Raka koi and Tilapia Farm, Denpasar. The samples taken were 19 samples, the 8 samples are thescale swabs from koi fish from Tulungagung and 11 scale swab samples from koi fish from Blitar using random sampling technique. Staphylococcus sp was isolated with Blood Agar and MacConkey Agar media and identified by Gram stain test and catalase test. From the results of the isolation and identification of bacteria, 1 sample was positive for Staphylococcus spp. (5.26%).

Co-Authors Aditya Nugraha Agus Setiyono Aida Lousie Tenden Rompis Anak Agung Sagung Rani Manika Putri Arifah, Sitti BOI DARMA Darmawan, Alpian Deny Rahmadani, Deny Ekowati Handharyani EMILIUS MELIANO UMBU RAZA Fachriyan Hasmi Pasaribu Fitri Indah Permatasari GUSTI AGUNG AYU ANGGRENI PERMATASARI I Gede Hendra Prasetya Wicaksana I Gede Oka Darsana I GEDE OKA DARSANA I Ketut Suada I Made Arthawan I Nengah Kerta Besung I Nyoman Dibia I Wayan Sudira K. Tono P.G Kadek Apriyan Widiarta KETUT SUADA Ketut Tono Pasek Gelgel Kristiawan, Vicky MADE SUMITHA KAMESWARI MADE SUMITHA KAMESWARI MADE YENDHI SAWITTI MARIA PRISTI ANRIS YUNIKAWATI Muh. Muazdzamzam Lil Abrori Muslimin, Lucia N. Suarsana Ni Made Herawati Putu Gede Widiarsa Putra Putu Henrywaesa Sudipa Retno Damayanti Soejoedono REZKI ABADI BONTONG Syafiana Fairizca Vera Paulina Sitanggang Vera Paulina Sitanggang WAODE SANTA MONICA

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