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All Journal Buletin Veteriner Udayana Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Advances in Tropical Biodiversity and Environmental Sciences Journal of Veterinary and Animal Sciences
Putu Henrywaesa Sudipa
Laboratorium Mikrobiologi Veteriner Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Identifikasi dan Prevalensi Jamur Curvularia pada Anjing dan Kucing di Kabupaten Badung, Bali Tahun 2020 Sudipa, Putu Henrywaesa; Gelgel, Ketut Tono Pasek; Jayanti, Putu Devi
Indonesia Medicus Veterinus Vol 10 (3) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.3.432

Abstract

Curvularia adalah genus Pleosporalean monophyletic dengan banyak jenis spesies, termasuk jenis fitopatogenik, jamur patogen pada hewan dan manusia. Curvularia juga menyebabkan phaeohyphomycosis yang mana ditemukan pada invertebrata, vertebrata berdarah dingin, burung dan spesies mamalia termasuk ruminansia, kuda, anjing, kucing dan manusia. Penelitian ini bertujuan untuk mengidentifikasi dan mengetahui prevalensi Curvularia pada anjing dan kucing. Sampel diambil dengan cara menggunakan teknik sitologi kulit dengan menggunakan plester selotip (tape) yang ditempelkan pada bagian tubuh yang mengalami infeksi jamur kemudian ditempelkan pada gelas objek yang sudah diberikan beberapa tetes pewarna methylene blue. Sampel menggunakan 34 sampel (26 ekor anjing dan 8 ekor kucing) dan sampel diperiksa dibawah mikroskop cahaya dengan pembesaran 100 kali. Pemeriksaan mikroskopis sampel melihat karakteristik jamur, terutama pigmentasi, septasi, morfologi, ukuran hifa, konidiofor dan konidia. Dari data jenis jamur yang teridentifikasi kemudian data ditabulasi dan dijelaskan secara deskriptif. Hasil yang didapatkan adalah prevalensi Curvularia pada anjing adalah 19%, pada kucing 50% dan total infeksi Curvularia dari seluruh sampel berjumlah 26%. Culvularia yang teridentifikasi adalah tipe konidia tipikal yang bentuk konidiumnya obovoid, melengkung, agak membengkak pada satu sel, gelap, dan dindingnya tebal dan tipe konidia atipikal yang bentuknya lurus dan menyempit ke arah ujung, konidiofor tidak bercabang, dan berbentuk zigzag serta jumlah septa berjumlah 3-4 atau eusepta.
Madu Trigona Mampu Menghambat Pertumbuhan Jamur Curvularia sp. yang Diisolasi dari Anjing Patabang, Denselina Lilis; Suartha, I Nyoman; Sudipa, Putu Henrywaesa
Indonesia Medicus Veterinus Vol 11 (1) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.1.117

Abstract

Curvularia sp. merupakan jamur dermatiaceae atau jamur dengan pigmen hitam yang menyebabkan berbagai macam penyakit pada hewan. Peningkatan invasi dan proliferasi jamur Curvularia sp. diakibatkan oleh penggunaan bahan kimia secara berlebihan. Oleh karena itu, dibutuhkan alternatif lain dalam mengobati penyakit jamur yaitu dengan obat herbal. Madu trigona mengandung senyawa flavonoid dan polyphenol karena lebah trigona dapat mengumpulkan nektar dari bagian bunga. Tujuan dari penelitian ini adalah untuk mengetahui tentang efektivitas madu trigona dalam menghambat pertumbuhan jamur Curvularia sp. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap terhadap enam perlakuan yaitu madu trigona konsentrasi 20%, 25%, 30% dan 100%, kontrol positif dan kontrol negatif terhadap biakan jamur Curvularia sp. pada media Sabouraud Dextrose Agar. Madu trigona kemudian di uji sensitivitasnya dengan teknik lubang sumuran pada media Sabouraud Dextrose Agar dan dilihat hingga terbentuknya zona hambat. Terbentuknya hambatan di sekitar lubang sumuran yang tidak ditumbuhi jamur menunjukkan hasil positif dan zona hambat dapat diukur. Data kemudian dianalisis dengan menggunakan sidik ragam. Hasil dari penelitian menunjukkan bahwa madu trigona konsentrasi 20%, 25%, 30% dan kontrol negatif tidak menghasilkan zona hambat (0 mm). Madu trigona konsentrasi 100% menghasilkan zona hambat sebesar 2,14 mm, sedangkan kontrol positif menghasilkan zona hambat sebesar 5,15 mm. Madu trigona yang dapat menghambat pertumbuhan jamur Curvularia sp. yaitu madu dengan konsentrasi 100%.
Bakteri Escherichia coli Teridentifikasi pada Rektum Lumba-Lumba Hidung Botol Indo-Pasifik di Umah Lumba Rehabilitation Center, Taman Nasional Bali Barat Kristiawan, Vicky; Mahatmi, Hapsari; Sudipa, Putu Henrywaesa; Rahmadani, Deny
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.234

Abstract

Saluran cerna merupakan salah satu tempat pertumbuhan berbagai genus serta spesies bakteri termasuk bakteri family Enterobacteriaceae. Salah satu dari anggotanya adalah Eschericia coli yang merupakan flora normal yang sering ditemukan pada saluran cerna. Saat ini masih sangat langka penelitian tentang keberadaan E. coli pada saluran cerna lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus). Tujuan penelitian ini adalah untuk mendapatkan data awal mengenai bakteri Enterobateriaceae pada lumba-lumba tersebut yang berada di lingkungan rehabilitasi. Sampel lumba-lumba yang digunakann merupakan lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di jaring apung laut atau seapen Umah Lumba Rehabititation Center, sebanyak tiga ekor. Pengambilan sampel dilakukan dengan menggunakan rubber catheter sterile berukuran 2,7 mm yang dimasukkan ke dalam rektum lumba-lumba. Jumlah total sampel yang di isolasi sebanyak 12 sampel dari tiga ekor lumba-lumba. Isolasi sampel dilakukan dengan menggunakan media Sheep Blood Agar, media Mac Conkey Agar dan Eosine Methylen Blue Agar. Selanjutnya di uji dengan pewarnaan Gram, uji katalase, uji oksidase, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, dan uji urease. Hasil dari penelitian ini dapat disimpulkan pada tiga ekor lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di seapen Umah Lumba Rehabilitation Center, ditemukan jenis bakteri famili Enterobacteriaceae spesies E. Coli.
Gambaran Radiografis Penggunaan Tulang Babi Sebagai Bahan Cangkok Untuk Penanganan Fraktur Femur Pada Anjing Luh Made Sudimartini; I Wayan Wirata; Anak Agung Gde Oka Dharmayudha; I Wayan Nico Fajar Gunawan; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.341 KB) | DOI: 10.24843/bulvet.2019.v11.i01.p04

Abstract

Fracture is one of the cases that may occur in pets, especially dogs and cats. The principle to treatment cases of fracture is repositioned and immobilized in the area of ??the fracture. Large bone damage due to trauma can inhibit healing process and cause bone defects, so that graft material is needed to stimulate the healing process and to fill in the missing bone. This research was aimed to study radiographic imaging in the use of pig’s bone as graft material for the treatment of fractures in dogs. Eight male dogs aged 3-4 months were used in this study and were divided into 2 groups randomly. Group I (control) was 2 dogs who their bone diaphysis femur was drilled with a diameter of 1 cm without giving graft material. Group II was 6 dogs who were drilled as Group I and were given a graft material. Monitoring the progress of healing process by rontgent, was conducted at 24 hours, 2nd week, 4th week and 8th week post-surgery. Radiographic analysis showed that there has been a unification and mineralization of bone fragments in the 8th week post-surgery in the group II with bone density already seemed normal.
Staphylococcus spp. pada Ikan Koi yang Dipelihara dalam Kolam Isolasi pada Masa Karantina Syafiana Fairizca; Hapsari Mahatmi; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.962 KB) | DOI: 10.24843/bulvet.2022.v14.i03.p13

Abstract

Currently, koi fish enthusiasts and fans in various regions in Indonesia, especially in Bali, have increased very sharply. In the conditions of transportation from outside Bali to Bali, it triggers stress which causes the koi's resistance to decrease so that disease occurs. Therefore, a quarantine process is very necessary to get koi that are healthy and suitable for cultivation. Diseases in koi fish can be caused by viruses, parasites, and bacteria, one of which is Staphylococcus spp. Research on bacterial infections in koi fish is still very rare, especially regarding Staphylococcus spp. in koi fish in Indonesia and in Bali. This study was conducted to obtain preliminary data regarding Staphylococcus sp infection in koi fish. In this study, the object used was koi fish which has any symptomps of diseases or died during a 14-day quarantine period.The researched koi fish came from suppliers in Tulungagung and Blitar, East Java. The size of the koi fish ranged from 15 -25 cm which was kept in fish isolation ponds at Raka koi and Tilapia Farm, Denpasar. The samples taken were 19 samples, the 8 samples are thescale swabs from koi fish from Tulungagung and 11 scale swab samples from koi fish from Blitar using random sampling technique. Staphylococcus sp was isolated with Blood Agar and MacConkey Agar media and identified by Gram stain test and catalase test. From the results of the isolation and identification of bacteria, 1 sample was positive for Staphylococcus spp. (5.26%).
Deteksi Bakteri Berpotensi Patogen Pada Penyakit Saluran Pernapasan Komplek Babi I Gusti Ketut Suarjana; I Nengah Kerta Besung; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.062 KB) | DOI: 10.24843/bulvet.2022.v14.i03.p11

Abstract

This study aims to detect bacterial infectious agents in pigs with complex respiratory tract disease known as porcine respiratory disease complex (PRDC) in some pig herds in Bali and look for the right medication is use to handle the incidence. The research sample comes from Tabanan, Badung and Gianyar totaling 48 Samples were isolated and identified by methods: isolation, the primary test, biochemical test. Test of sensitivity to antibiotics using disc diffusion method according to Kirby-Bauer method. The result showed that bacterial agents isolated from pigs were among others Streptococcus spp. alfa hemolytic non-coagulase (41,6%), Staphylococcus spp. beta hemolytic noncoagulase (41,6%) and Escherichia coli. Staphylococcus spp. beta hemolytic non-coagulase and E. coli were sensitive to streptomycin, kanamycin, doxicyclin, clindamycin and bacitracin. To conclude, Streptococcus spp. alfa hemolytic non-coagulase were sensitive to streptomycin, kanamycin, clindamycin and bacitracin, but resisten to doxicyclin.
Pengaruh Konsentrasi dan Lama Perendaman Air Perasan Daun Pegagan terhadap Kualitas Daging Babi Ditinjau dari Uji Organoleptik Putu Henrywaesa Sudipa; Ida Bagus Ngurah Swacita; I Made Sukada; Luh Made Sudimartini; Kadek Karang Agustina
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.144 KB) | DOI: 10.24843/bulvet.2020.v12.i02.p12

Abstract

Almost all meat on the market is contaminated by microorganisms. As a result of the contamination of microorganisms, it changes in color, odor, and consistency. This research aims to study the effect of various levels of concentration and soaking time of Pegagan (Centella asiatica L.) leaf juice and to study whether there is an interaction between concentration and soaking time of Centella asiatica L. juice on the quality of pork in terms of the organoleptic test. Soaking pork in the juice of Centella asiatica L. juice is done by inserting each piece of meat into eight glasses, which have been filled with control, 5%, 10%, and 15% concentration juice. Soaking is done for 5 minutes, and 10 minutes then the meat is drained for 15 minutes, then an organoleptic test is performed and analyzed by variance. The results of the concentration had a significant effect (P<0.05) on changes in color, odor, and consistency of meat. While the soaking time had a significant effect (P<0.05) on the smell of meat but no significant effect (P>0.05) on the color and consistency of pork. And also, there is no interaction between concentration and soaking time.
Efikasi Sterilisasi dan Desinfeksi Kandang untuk Mengurangi Infeksi Bakteri Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.888 KB) | DOI: 10.24843/bulvet.2020.v12.i01.p11

Abstract

Biosecurity efforts on chicken farms such as using antiseptics need to be modified so that the aim of suppressing the bacteria in the cage is effective. The purpose of this research is to find alternative methods for sterilization and disinfection of broiler cages and to find out the number and type of bacterial contamination in broiler cages. In this study two cages were used, the first cage washed with detergent and given Povidon Iodine disinfectant. In the second cage after washing with detergent, the floor of the cage is burned/heated and the cage wall is disinfected. Then the blood agar media is placed on the cage’s floor and opened for 1 hour. Then the blood agar media examined in the laboratory to see the number and type of bacteria that grow. All samples were tested with T-Test. The results showed that disinfection with disinfectants had more number of colonies (206 colonies, with an average of 29.85 colonies) than cages that used a heating method with a stove (182 colonies, with an average of 26 colonies). The results show there is no significant difference between sterilization treatment and disinfection treatment. The method of sterile sterilization by heating using a stove is slightly more effective than disinfection by using disinfectants seen from the number of bacterial colonies that grow, although the difference between the two methods is very little.
Ekspresi Gen Reseptor Growth Hormon pada Otot Sapi Bali dan Sapi Wagyu Ni Ketut Suwiti; Putu Henrywaesa Sudipa; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.991 KB) | DOI: 10.24843/bulvet.2022.v14.i02.p02

Abstract

This study aims to study the molecular weight and distribution of protein growth hormone receptors (GHR). The sample used was the Balinese beef eye ribs and Wagyu beef muscle. Molecular weight examination using the SDS-PAGE receptor method, Western Blotting, and immunohistochemistry staining. The results showed that the weight of the growth hormone receptor protein was 76 Kdalton in both types of cattle and the average score of IHK for Wagyu beef muscle was 234.5 and in Bali cattle was 199.6. It can be concluded, namely the weight molecule of the growth hormone receptor muscle ribs in both Wagyu and bali cattle with equal distribution.
Malassezia sp. Infection Prevalence in Dermatitis Dogs in Badung Area Putu Henrywaesa Sudipa; Ketut Tono Pasek Gelgel; Putu Devi Jayanti
Advances in Tropical Biodiversity and Environmental Sciences Vol 5 No 2 (2021): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.842 KB) | DOI: 10.24843/ATBES.2021.v05.i02.p02

Abstract

Malasseziosis is a common fungal infection in dogs and it is secondary to the initial underlying dermatitis infection. These infections can worsen the prognosis of disease in dogs. The study was conducted on 26 dogs that were treated in several veterinary clinics in the Badung, Bali. This study aimed to determine the incidence rate of Malassezia sp. in dogs with dermatitis. Samples were collected using tape smear method from the skin lesion and then by microscopic examination. The results were tabulated and analyze descriptively. The results showed that 15 of the 26 samples of dogs tested positive were infected with Malassezia sp. (58%). Infection is more common in male (60%) and geriatric dogs (40%). Lesions were more common in the ear, limb, vaginal and inguinal region of infected dogs
Co-Authors Anak Agung Gde Oka Dharmayuda Anak Agung Gde Oka Dharmayudha Deny Rahmadani, Deny Gunawan, I Wayan Nico Fajar Hapsari Mahatmi I Gusti Ketut Suarjana I Ketut Puja I Made Sukada I Nengah Kerta Besung I Nyoman Suartha I Wayan Sukernayasa I Wayan Wirata Ida Bagus Ngurah Swacita Kadek Karang Agustina Ketut Tono Pasek Gelgel Kristiawan, Vicky Luh Made Sudimartini Muh. Muazdzamzam Lil Abrori Ni Ketut Suwiti Ni Luh Eka Setiasih Patabang, Denselina Lilis Putu Devi Jayanti Romy Muhammad Dary Mufa Syafiana Fairizca