Hapsari Mahatmi
Laboratorium Bakteriologi Dan Mikologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234

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Escherichia coli pada Swab Rektum Ikan Koi yang Dipelihara di Kolam Isolasi pada Masa Karantina Kadek Apriyan Widiarta; Hapsari Mahatmi; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.751 KB) | DOI: 10.24843/bulvet.2022.v14.i02.p15

Abstract

Koi fish (Cyprinus carpio) is one of the freshwater ornamental fish commodities which has a very high economic value and is of high interest. Koi fish are generally transported by land. This condition can cause koi to become stressed, which has an impact on decreasing health and even disease errors. One indicator of koi health problems can be seen from the presence of E. coli. This study aims to see the Escherichia coli bacteria in koi fish kept in isolation ponds during the quarantine period using 19 samples of isolates from rectal swabs which were then taken to BBVET Denpasar for isolation and identification of E. coli bacteria. This study used an exploratory observation design with a cross-sectional method, while the sample was taken by purposive sampling. The data obtained were descriptive qualitative. The result of this study are known from 19 samples that be examined show five samples positive of Escherichia coli bacteria (26,3%) which is three samples (15,9%) came from Tulungagung koi and two samples (10,3%) came from Blitar koi.
Cemaran Bakteri dan Residu Antibiotika Daging Babi di Pasar Tradisional Kecamatan Abiansemal dan Kuta Kabupaten Badung I Made Arthawan; Hapsari Mahatmi; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.163 KB) | DOI: 10.24843/bulvet.2021.v13.i02.p04

Abstract

This study aimed to determine the bacterial contamination and antibiotic residues on pork and provided an overview of the handling conditions of pork sold in wet markets in the Abiansemal and Kuta, Badung traditional markets. This study was a cross-sectional study using questionnaires and testing bacterial contamination and antibiotic residues in pork. Questionnaires and pork samples were collected from 26 pork sellers from 8 wet markets, then the data were discussed descriptively. Bacterial contamination tests were carried out by calculating the value of Total Plate Count, Coliform, Escherichia coli, Salmonella sp, Staphylococcus aureus, and Campylobacter sp. The antibiotic residue test was carried out by a screening method (bioassay) against tetracycline and penicillin antibiotics. The results showed that the mean total plate count was 3,7 x 105 (7,3 x 103 to 1,5 x 106 colonies/gram). The contamination of Coliform and Escherichia coli were 1,3 x 105 dan 2,1 x 104 respectively with (1,1 x 103 to 4,4 x 105 colonies/gram) and (4,5 x 101 to 9,7 x 104 colonies/gram). Contamination of Staphylococcus aureus was the negative results up to 2 x 101 colonies/gram. Contaminations of Salmonella sp and Campylobacter sp were negative. Tetracycline and penicillin antibiotic residues were found in 15.38% and 11.54% of all pork samples. The level of public understanding and awareness of the handling and sanitation of pork was found in 50% of respondents who were in the adequate category and 50% in the categories lacked understanding and were less aware of sanitation. Factors that influence the level of bacterial contamination were hygiene practices and sanitation practices of pork sellers 7.7% in the good category, 80.8% in the moderate category and 11.5% in the low category. In conclusion, pork in the traditional markets of Abiansemal and Kuta in Badung Regency is contaminated with bacteria and contains antibiotic residues, and from the aspect of veterinary public health, it was not suitable for consumption.
Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER Hapsari Mahatmi; Agus Setiyono; Retno Damayanti Soejoedono; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.113 KB)

Abstract

A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.
Faktor yang Mempengaruhi Perilaku dan Persepsi Dokter Hewan Bali Terhadap Penggunaan Antimikroba dan Resistensi Antimikroba Vera Paulina Sitanggang; I Nngah Kerta Besung; Hapsari Mahatmi
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p13

Abstract

Antimicrobial resistance (AMR) is the ability of bacteria to withstand the effects of drugs so that bacteria do not die after the administration of antimicrobials and the function of the drug does not work at all. This incident is caused by the uncontrolled use of antimicrobials which can cause a global crisis on human and animal health in the future. This study aims to determine the factors that influence the behavior and perceptions of veterinarians in the province of Bali on the use of antimicrobials and antimicrobial resistance. A total of 204 veterinarians were used as research samples through a survey using a Likert system and then the results of differences in veterinary responses to the frequency of prescribing were analyzed by Kruskal-Wallis. The results showed that antimicrobials were given to half of the patients treated weekly, and the factors that influenced the administration of antimicrobials were the history of antimicrobial use, level of safety, experience, potency, side effects, and the risk of increasing antimicrobial resistance. The cost of culture testing and the price of antimicrobials are the biggest barriers for small animal veterinarians but not for livestock veterinarians. All respondents acknowledged that most sources of information on AMR were easy to obtain and useful for them. The group of veterinarians who practice small animals and livestock practitioners agree that AMR is a serious common threat in the future. This study provides important insights into the application of veterinary medical procedures in the province of Bali and will later become a reference in increasing regulation control over the use of antimicrobials.
Infeksi Bakteri Aeromonas sp. pada Ikan Koi Masa Karantina Anak Agung Sagung Rani Manika Putri; Hapsari Mahatmi; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p11

Abstract

Bacteria Aeromonas sp. is one type of pathogenic bacteria in humans and can cause disease in animals. These bacteria also attack various species of freshwater fish, one of which is the koi fish. The purpose of this study was to obtain preliminary data on the infection ofbacteria Aeromonas sp. in koi fish (Cyprinus carpio L), which has the potential to cause disease in koi fish in Bali, especially koi fish that have just been imported from outside the island of Bali during the quarantine period. A total of 19 koi fish measuring 15-25 cm are suspected of being infected with bacterial diseases and dead koi fish swab was done on the scales and gills, then the sample was isolated and identified bacteria at the Balai Besar Veteriner. Water temperature and pH are measured to determine water quality. The data obtained are presented descriptively. The results showed that one sample (5.26%) was infected with Aeromonas sp.
Kualitas Daging Kerbau Beku Asal India Ditinjau dari Cemaran Salmonella spp. di Pasar Aikmel, Kecamatan Aikmel, Kabupaten Lombok Timur, Provinsi Nusa Tenggara Barat Muh. Muazdzamzam Lil Abrori; Hapsari Mahatmi; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p06

Abstract

Since 2015 imports of frozen buffalo meat from India have begun to enter Indonesia, especially in West Nusa Tenggara, as an effort to meet market needs in modern shopping areas in Lombok City. The price of frozen buffalo meat is relatively cheap, causing marketing to expand to the East Lombok area, especially the Aikmel traditional market, which is about 72 km away. This study aims to determine the presence of bacterial contamination of Salmonella spp. on frozen packaged buffalo meat from India sold at Aikmel Market. The research sample was buffalo meat from 10 sellers, each weighing 100 grams. Then isolation and identification were carried out. Bacterial isolation was carried out using selective media, namely Xylose Lysine Deoxycholate, Gram staining, Biochemical Test which included Triple Sugar Iron Agar, Indole, Methyl Red, Voges Proskauer, Citrate and Urease tests. The results showed that samples of frozen buffalo meat marketed in the Aikmel market 9 samples of 10 samples of buffalo meat (90%) showed contamination of Salmonella spp. Based on the results of this study, it is necessary to increase local government supervision of the procedures for selling frozen buffalo meat from India.
Deteksi Residu Antibiotik pada Daging Ayam Broiler dan Itik Serta Tingkat Kesadaran Peternak di Wilayah Selemadeg Timur Tabanan Bali Fitri Indah Permatasari; I Nengah Kerta Besung; Hapsari Mahatmi
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p17

Abstract

Public consumption of chicken meat, especially broiler and duck, continues to increase. Various efforts have been taken by farmers to increase their production by increasing the quantity of their livestock and improving livestock management. The government has banned the use of antibiotics under Law no. 41 of 2014. The existence of this law needs to be evaluated on livestock products produced. This study aims to determine the presence of antibiotic residues and also to determine the level of awareness of farmers to antibiotic residues in broiler chicken and duck meat in the Selemadeg Timur Tabanan of ??Bali. This research is a descriptive study by detecting samples of chicken and duck meat using bioassays at BBVet Denpasar and by giving questionnaires to farmer. The number of samples was 10 samples of chicken meat, 2 samples of duck meat, and 15 samples kuesioner. Based on the results of the bioassay test, it was found that all samples were negative for residues of tetracycline antibiotics, aminoglycosides, macrolides, and penicillin. In addition, based on the results of questionnaire data, the level of public awareness of the use of antibiotics and the dangers of antibiotic residues in meat is very high. So that chicken meat in Selemadeg Timur ??Tabanan Bali is still said to be safe for consumption. However, there is still a need for supervision from the government and veterinarians regarding the use of antibiotics so that the quality of livestock products is maintained.
Sejumlah Faktor yang Melandasi Persepsi dan Perilaku Dokter Hewan terhadap Resistansi Antimikrob dan Penggunaan Antimikrob Vera Paulina Sitanggang; I Gede Hendra Prasetya Wicaksana; I Nengah Kerta Besung; Hapsari Mahatmi
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.424

Abstract

Antimicrobial resistance (AMR) is the ability of bacteria to withstand the effects of drugs so that bacteria do not die after the administration of antimicrobials and the function of the drug does not work at all. This incident is caused by the uncontrolled use of antimicrobials which can cause a global crisis on human and animal health in the future. This study aims to determine the factors that influence the behavior and perceptions of veterinarians in the province of Bali on the use of antimicrobials and antimicrobial resistance. A total of 204 veterinarians were used as research samples through a survey using a Likert system and then the results of differences in veterinary responses to the frequency of prescribing were analyzed by Kruskal-Wallis. The results showed that antimicrobials were given to half of the patients treated weekly, and the factors that influenced the administration of antimicrobials were the history of antimicrobial use, level of safety, experience, potency, side effects, and the risk of increasing antimicrobial resistance. The cost of culture testing and the price of antimicrobials are the biggest barriers for small animal veterinarians but not for livestock veterinarians. All respondents acknowledged that most sources of information on AMR were easy to obtain and useful for them. The group of veterinarians who practice small animals and livestock practitioners agree that AMR is a serious common threat in the future. This study provides important insights into the application of veterinary medical procedures in the province of Bali and will later become a reference in increasing regulation control over the use of antimicrobials.