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All Journal Dentino: Jurnal Kedokteran Gigi Dentin Prosiding Konferensi Nasional PKM-CSR
Irham Taufiqurrahman
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Humanities Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Environmental Science Nursing Public Health Social Sciences

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ANTIBACTERIAL ACTIVITY OF CHITOSAN FROM HARUAN (Channa striata) FISH SCALES AGAINST THE GROWTH OF Porphyromonas gingivalis Rahma Dania A.J; Deby Kania Tri Putri; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8122

Abstract

Background: Periodontal disease is one of the dental and oral diseases that is often found in people in Indonesia. RISKESDAS (2018) stated that the prevalence of people who have dental and oral health problems in Indonesia is 57.6%. The prevalence of oral and dental problems in South Kalimantan is 60%. Periodontal disease is damage to the supporting tissues of the teeth caused by specific microorganisms such as Porphyromonas gingivalis which are gram negative bacteria. The growth of these bacteria can be inhibited and killed by chitosan from haruan fish scales. Chitosan is a natural product of chitin, chitosan is the basic ingredient of fish scales. Chitosan has advantages and more safety levels, because it has an active group that will bind to microbes so chitosan can inhibit and kill microbial growth. Objective: to analyze the antibacterial activity of chitosan scales (Channa striata) on the growth of Porphyromonas gingivalis. Methods: This research used true experimental with randomized pre and post test with only control group design with eight treatments. Chitosan of haruan fish scales with liquid dilution method to obtain MIC and solid dilution test to obtain MBC. Results: The results showed that Minimum Inhibitory Concentration (MIC) of chitosan haruan scales was found at a concentration of 2.5% characterized by a decrease in absorbance value of 0.092 and Minimum Bactericidal Concentration found at a concentration of 20% characterized by the absence of Porphyromonas gingivalis. Conclusion: There is an inhibitory activity and a bactericidal activity from chitosan of haruan fish scales on Porphyromonas gingivalis.
PHYTOCHEMICAL AND CYTOTOXICITY TESTING OF RAMANIA LEAVES (Bouea macrophylla Griffith) ETHANOL EXTRACT TOWARD VERO CELLS USING MTT ASSAY METHOD (Preliminary study of adjuvant therapy materials to the preparation of the drug) Laila Fitri; Irham Taufiqurrahman; Irnamanda DH
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4617

Abstract

Background: Ramania leaves (Bouea macrophylla Griffith) are one of the local medicinal plants of South Kalimantan which is used as adjuvant therapy material. Previous research showed that ramania leaves ethanol extract showed that the extract has potential as adjuvant therapy material for anticancer, but the safety of this material is not yet known so it  needs to be tested with cytotoxicity test to know the toxic properties of the material toward normal cells in vitro. Objective: This research aims to determine the cytotoxicity properties of ramania leaves ethanol extract toward Vero cells using MTT assay method. Method: It was a true experimental research using posttest-only with control group design consist of 8 treatment groups: 31.25μg/mL, 62.5μg/mL, 125μg/mL, 250μg/mL, 500μg/mL, 1000μg/mL, 2000μg/mL and 4000μg/mL and 2 control groups which were cell control and media control. Study phases include phytochemical test, tannic acid test, cell culture, harvest and cell calculation and cytotoxicity test. The result test was read by ELISA reader, the absorbance will be calculated  by a  formula. Results: The test result showed that ramania leaves ethanol extract contained secondary metabolite such as phenol, flavonoid, steroid and terpenoid. The identification test of tannic acid compound  using TLC method (thin layer chromatography) showed the presence of tannic acid compound.The probit analysis showed IC50in 35.808μg/mL. Conclusion: Based on the research, it can be concluded that ramania leaves ethanol extract are found cytotoxic toward Vero cells after the analysis using MTT assay method (IC50<100μg/mL).
ANTIOXIDANT ACTIVITY OF BINJAI LEAVES(Mangifera caesia) ETHANOL EXTRACTS (Research report) Anita Diana Putri; Irham Taufiqurrahman; Nurdiana Dewi
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6176

Abstract

Background: Binjai is one of Mangifera species which commonly found in South Kalimantan. Binjai leaves are known to contain flavonoids compounds, that have an effect as antioxidant that can accelerate wound healing process after tooth extraction. This study was conducted to examine the variation of  solvent concentration towards antioxidant activity of Binjai’s leaves extract using maceration method. Antioxidant activity is tested with DPPH (2,2-diphenyl-1-picrylhydrazyl) and examined using Spectrophotometer UV-Vis. Purpose: This study aims to determine solvent concentration which exxpres optimal antioxidant activity of Binjai leaves extract. Methods: This study is a true experimental with post-test only control group design. The sampling technique of Binjai leaves was determined by simple random sampling and 7 samples was opted for each treatment. Results: The study revealed that 96% ethanol extract of Binjai leaves obtained IC50 (Inhibitory Concentration) as much of 16.14 ppm (very active), 70% ethanol extract of the Binjai leaves obtained 37.94 ppm (very active), and 50% ethanol extract of Binjai leaves obtained 58.07 ppm (active). Data analysis was conducted using One Way ANOVA parametric test and LSD post hoc test which demonstrated a significant difference among 96%, 70% and 50% of ethanol  extracts of Binjai leaves with p = 0.000 (p <0.05). Conclusion: Binjai leaves extracted using 96% ethanol solvent possesses higher level of antioxidant activiy compared to 70% and 50% ethanol concentration.
ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA Dita Puspita Sari; Didit Aspriyanto; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8118

Abstract

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.
ANTIBACTERIAL ACTIVITIES OF CHITOSAN IN HARUAN FISH SCALES (Channa striata) TO THE GROWTH OF Staphylococcus aureus Dewi Retno Wahyu Widyaningrum; Deby Kania Tri Putri; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7045

Abstract

Background: Haruan fish scales (Channa striata) contain chitin which can be used as antibacterial ingredients by processing it into chitosan. Chitosan contains an amino polysaccharide group which can inhibit the growth of Staphylococcus aureus, one of the opportunistic bacteria, which becomes a pathogen if there is an imbalance within oral environment and one of the causes of periodontal disease. Besides, these bacteria are also reported to be resistant to conventional antibacterial agents. Objective: The purpose of this study was to find out and elaborate the difference in antibacterial activity of chitosan from haruan fish scales (Channa striata) at the concentration of 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus. Methods: This study used a randomized pretest-posttest with control group design and One Way ANOVA statistical test was performed and was followed by Post Hoc Dunnet test. The procedure of this study consisted of chitosan fabrication scales from fresh fish by deproteination, demineralization, and deacetylation, followed by an anti-bacterial test with 10 treatment groups namely 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus to obtain Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The results of this study presented chitosan at 1.5% concentration as the Minimum Inhibitory Concentration (MIC) and 3.5% concentration as the Minimum Bactericidal Concentration (MBC) on the growth of Staphylococcus aureus. Evaluated from the difference in absorbance, 2.5% chitosan concentration showed superior activity in inhibiting Staphylococcus aureus compared to 0.2% chlorhexidine gluconate. Conclusion: It can be concluded that there are differences in the antibacterial activity of chitosan scales (Channa striata) with a concentration of 0, 5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus.
COMPARISON OF ANTIOXIDANT ACTIVITY BETWEEN SOCLETATION AND MACERATION EXTRACTION METHOD ON BINJAI LEAF EXTRACT (Mangiferacaesia) Norliyanti Norliyanti; Irham Taufiqurrahman; Bayu Indra Sukmana
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5376

Abstract

Background :Wound is known to generate free radicals in inflammatory phase by the initiation of inflammatory cells such as polymorphonuclear. This cell is in function to destroy bacteria and viruses that are present in the wound. However, free radicals can also damage normal tissues when the amount of the accumulation is too much. Antioxidants are the ingredients that can eliminate free radicals throughchemical reaction so the formation of free radicals can be reduced. Binjai (Mangiferacaesia) is a plant that can produce natural antioxidants that are able to protect the body against the damage which caused by free radicals. The antioxidants in the leaves can be obtained by maceration and socletation extraction techniques. Purpose: To determine the difference between socletation and maceration extraction method towards antioxidant activity of binjai leaf ethanol extract as a preliminary study of binjai leaf antioxidant effect on wound healing. Methods: This research was pure experimental study(true experimental) with post-test only control group design, consisting of two treatment groups: maceration extraction methods group, socletation extraction methods group, and one control of ascorbic acidgroup. Measurement of antioxidant activity using DPPH method were measured by using a UV-Visspectrophotometry then IC50 values were calculated to determine the antioxidant activity. Results: Average IC50 values obtained  fromthe socletation extract group was 50.791 ppm, while the maceration group was 60.135 ppm, and the control group was 13.825 ppm. Post Hoc LSD test result showed that the three groups had significant difference in each treatment.Conclusion:Based on this research, it can be concluded that binjai leaf extraction using socletation methods produce more powerful antioxidant activity compared to maceration method.
THE EFFECT OF MAULI BANANA (Musa acuminata) STEM EXTRACT GEL APPLICATION WITH 37.5% CONCENTRATION ON FIBROBLAST CELL COUNT (In Vivo Study On Wound Healing Process of Male Wistar Rat (Rattus norvegicus) Buccal Mucosa) Moh. Ihsan Rifasanto; Maharani Laillyza Apriasari; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4611

Abstract

Background: Wound healing is the body process to improve tissue intergrity caused by wound. Mauli banana stem is a potential plant which can be used as a medicine to accelerate wound healing. Mauli banana stem extract has some contents such as tannin and saponin which has immunomodulatory properties. Previous study states that mauli banana stem extract gel with 37,5% concentration can accelerate the healing of traumatic ulcers. Purpose: To analyze the effect of mauli banana stem extract gel application with 37,5% concentration on fibroblast cell count in wound healing process of oral mucosa wistar rat on the 7th day. Material and Methods: This study was a true experimental design with posttest only control group design. It consisted of three treatment groups: a group which given mauli banana (Musa acuminata) stem extract gel with 37,5% concentration, hydroxypropyl methylcellulose gel (negative group) and patent drug gel containing Aloe vera (positive group). Result: The mean value of fibroblast cell count in wound healing process on the 7th day of the treatment group (28,57), negative group (20,14), and positive group (23). One-way Anova’s test had shown a significant difference. Post Hoc LSD test showed a significant difference between mauli banana stem extract gel with 37,5% concentration with hydroxypropyl methylcellulose gel and patent drug gel containing Aloe vera. Conclusion: The application of mauli banana stem extract gel with 37,5% concentration can increase fibroblast cell count on the 7th day in wound healing process of oral mucosa.
TOTAL FLAVONOID CONTENT ANALYSIS OF RAMANIA LEAVES’ EXTRACT USING ETHANOL, METHANOL AND N-HEXANE AS SOLVENTS (Research report) Risa Hermalinda; Irham Taufiqurrahman; Zairin Noor Helmi
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6177

Abstract

Background: Wound healing process consists of three phases: inflammation, proliferation, maturation and remodelling. Secondary metabolites are chemical compounds that have potential to be explored and developed in search of raw materials for drugs to assist wound healing process; one of them is flavonoid. Flavonoid is classified as natural phenolic compound that has antioxidant properties coexisted with its antimicrobial and anti-inflammatory effect. Flavonoid is usually available in the form of glycosides and soluble in polar solvents, such as methanol and ethanol. The bark and leaves of Ramania, a native plant of Kalimantan, contains flavonoid compound with non-optimal utilization of its potential. Purpose: To determine the differences in total flavonoid content of Ramania leaves’ extract obtained using ethanol and methanol solvents. Methods: This study is a true experimental study using post-test only with control group design; Samples collection were comprised of 27 specimens, categorized into three groups: two treatment groups using 70% ethanol solvent, one group using 70% methanol solvents, and one control group using 70% n-hexane solvent. Results: There were significant differences between ethanol group and methanol group result (p<0.05); ethanol group and n-hexane group result (p<0.05); and methanol group and n-hexane group result (p<0.05). Conclusion: This study concluded that there were significant differences in the total flavonoid content of Ramania leaves’ extract using ethanol and methanol as its solvents.
THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS Dinda Rizkia; Irham Taufiqurrahman; Renie Kumala Dewi
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10648

Abstract

ABSTRACTBackground: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel
THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY Nabilah Nabilah; Irham Taufiqurrahman; Nolista Indah Rasyid
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11999

Abstract

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.
Co-Authors Adela Tamara Alexander Sitepu Ana Azizah Ansari Anita Diana Putri Aulia Rahman Bayu Indra Sukmana Beta Widya Oktiani Desty Ayu Dwiyanti Dewi Nurdiana Dewi Retno Wahyu Widyaningrum Didit Aspriyanto Didy Adham Dinda Rizkia Dita Puspita Sari Edyson Edyson Erida Wydiamala Gusti Rifda Aqiila Hatta, Isnur Hilyatul Auliya I Wayan Arya Krishnawan Firdaus Ika Kusuma Wardani Irnamanda DH Johay Maulida Rosita Laila Fitri Maharani Laillyza Apriasari Makfiyah Saidah Maulida Hasanah Maulida Syafarina Mery Novita Moh. Ihsan Rifasanto Monica Thiodora Limay Nabilah Nabilah Nolista Indah Rasyid Noor Hildayanti Norliyanti Norliyanti Novia Damayanti Rahma Dania A.J Rahmad Arifin Renie Kumala Dewi Rezky Muliyawan Ridha Aulia Risa Hermalinda Rosihan Adhani, Rosihan Salma Humaira Sari, Galuh Dwinta Sofyan Erwandi Syifa Ennisa Tri Putri, Deby Kania Utami, Juliyatin Putri Zairin Noor Helmi