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Manajemen instalasi forensik di rumah sakit Kepolisian Negara Republik Indonesia sebagai acuan pembentukan instalasi forensik kedokteran gigiManagement of forensic installations at Indonesian National Police Hospital as a reference for the establishment of forensic odontology installation at the Oral Hospital Firstady Widyarnan Munandar; Fahmi Oscandar; Yuti Malinda; Murnisari Dardjan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 28, No 3 (2016): Desember
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Instalasi forensik seharusnya terdapat bukan hanya di rumah sakit umum, tetapi juga di Rumah Sakit Gigi dan Mulut untuk menindaklanjuti kasus yang membutuhkan ilmu forensik kedokteran gigi. Tujuan penelitian adalah mempelajari gambaran unsur manajemen instalasi forensik di Rumah Sakit Kepolisian Pusat Sukanto, Rumah Sakit Bhayangkara Sartika Asih serta Laboratorium dan Klinik Odontologi Kepolisian sebagai acuan pembentukan Instalasi Forensik Kedokteran Gigi. Metode: Penelitian ini lakukan secara deskriptif dengan metode observasi. Lokasi penelitian di Rumah Sakit Kepolisian Pusat Sukanto, Rumah Sakit Bhayangkara Sartika Asih, Laboratorium dan Klinik Odontologi Kepolisian. Hasil: Dari hasil penelitian menunjukan Instalasi Forensik Rumah Sakit Kepolisian Pusat Tingkat I Sukanto, Rumah Sakit Bhayangkara Sartika Asih serta Laboratorium dan Klinik Odontologi Kepolisian memiliki banyak kesamaan dari aspek unsur manajemen. Simpulan: Manajemen Instalasi Forensik yaitu Rumah Sakit Kepolisian Pusat Sukanto, Instalasi Forensik Rumah Sakit Bhayangkara Sartika Asih serta Instalasi Forensik Odontologi Laboratorium memiliki banyak kesamaan dari segi unsur manajemen dan dapat menjadi acuan untuk pembentukan unsur manajemen instalasi forensik kedokteran gigi.Kata kunci: Unsur manajemen, instalasi forensik, instalasi forensik kedokteran gigi. ABSTRACTIntroduction: Forensic installations should be available not only in public hospitals, but also at the Dental and Oral Hospital to follow up on cases that require dental forensic science. The purpose of the study was to study the description of elements of forensic installation management at the Sukanto Hospital, Central Police, Bhayangkara Sartika Asih Hospital and the Police Odontology Laboratory and Clinic as a reference for the establishment of a Dentistry Forensic Installation. Methods: This research is done descriptively by observation method. Research locations at Sukanto Hospital, Central Police Department, Bhayangkara Sartika Asih Hospital, Police Odontology Laboratory and Clinic. Results: From the results of the study showed the Forensic Installation of the Central Police Hospital Sukanto, Bhayangkara Sartika Asih Hospital and the Police Odontology Laboratory and Clinic have a lot in common with aspects of management. Conclusion: Management of forensic Installations, Sukanto Hospital Central Police Hospital, Bhayangkara Sartika Asih Hospital Forensic Installation police have many similarities in terms of management elements and can be used as a reference for the management of dentistry forensic installations.Keywords: Management elements, forensic installation, odontology forensic installation.

Perbedaan pola rugae palatina sebelum dan sesudah perawatan dengan alat ortodonti lepasanDifferences in palatal rugae patterns before and after the removable orthodontic appliances treatment Sintia Saputra; Endah Mardiati; Indra Mustika Setia Pribadi; Yuti Malinda
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Pola rugae palatina memiliki karakteristik yang unik pada setiap individu, yang dapat dijadikan sarana identifikasi individu di bidang forensik kedokteran gigi, namun berbagai kontraversi muncul mengenai karakteristik rugae palatina secara kualitatif dan kuantitatif sesudah dilakukan perawatan ortodonti. Tujuan penelitian ini adalah untuk mengetahui perbedaan pola rugae palatina sebelum dan sesudah perawatan dengan alat ortodonti lepasan. Metode: Penelitian bersifat observasional dengan sampel penelitian terdiri dari 111 model studi sebelum dan sesudah dilakukan perawatan ortodonti. Teknik sampling adalah purposive sampling, dari pasien maloklusi dento-alveolar kelas I, usia 18-30 tahun di RSGM FKG Unpad. Data penelitian dideskripsikan dan dianalisis dengan uji statistik Wilcoxon (ɑ = 0,05) untuk mengetahui perbedaan pola rugae palatina sebelum dan sesudah perawatan ortodonti. Hasil: Hasil penelitian menunjukkan, ukuran rugae palatina sebelum dilakukan perawatan terbanyak adalah rugae primer 85,2%, rugae sekunder 13,4%, rugae fragmen 1,37%, sesudah perawatan rugae primer 85.5%, rugae sekunder 13,2%, rugae fragmen 1,3%. Berdasarkan arah, rugae palatina sebelum perawatan arah postero-anterior 44,5%, antero-posterior 38,6%, sesudah perawatan arah posterior-anterior 44,9%, antero-posterior 38,3%. Arah perpendikular 8,7% dan berbagai arah 8,2% baik sebelum maupun sesudah perawatan. Uji beda menunjukan, tidak terdapat perbedaan signifikan sebelum dan sesudah perawatan ortodonti. Simpulan: Pola rugae palatina yang sering muncul sebelum dan sesudah perawatan ortodonti adalah rugae primer dengan arah postero-anterior, tidak terdapat perbedaan signifikan sebelum dan sesudah perawatan ortodonti.Kata kunci: Forensik odontologi, perawatan ortodonti, pola rugae palatina. ABSTRACTIntroduction: Palatal rugae patterns have unique characteristics in each individual, which can be used as a means of identifying individuals in the field of dentistry forensics, but various contraceptives arise about the characteristics of palatal rugae qualitatively and quantitatively after orthodontic treatment. The purpose of this study was to determine the differences in palatal rugae patterns before and after treatment with removable orthodontic devices. Methods: The study was observational with a study sample consisting of 111 study models before and after orthodontic treatment. The sampling technique was purposive sampling, from class I dento-alveolar malocclusion patients, aged 18-30 years at FKG Unpad Hospital. The research data was described and analyzed by Wilcoxon statistical test (ɑ = 0.05) to determine differences in palatal rugae patterns before and after orthodontic treatment. Results: The results showed that the size of palatal rugae before treatment was the primary rugae (85.2%), secondary rugae (13.4%), fragment rugae (1.37%), after primary rugae treatment (85.5%), secondary rugae (13.2%) , rugae fragment (1.3%). Based on direction, palatal rugae prior to postero-anterior (44.5%), antero-posterior (38.6%) treatment, after posterior-anterior (44.9%), antero-posterior (38.3%) treatment. Perpendicular direction (8.7%) and various directions (8.2%) both before and after treatment. Different tests showed that there were no significant differences before and after orthodontic treatment. Conclusion: The palatal rugae pattern that often occurs before and after orthodontic treatment is the primary rugae with postero-anterior direction, there are no significant differences before and after orthodontic treatment.Keywords: Odontological forensics, orthodontic treatment, palatal rugae pattern.

Perbedaan jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokokDifference between the number of Streptococcus mutans colonies in kretek smokers and non-smokers Intan Melani; Mieke Hemiawati Satari; Yuti Malinda
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 30, No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Rokok kretek merupakan jenis rokok yang paling populer di Indonesia. Merokok dapat menyebabkan berbagai gangguan dalam rongga mulut, salah satunya yaitu karies. Streptococcus mutans merupakan salah satu bakteri yang berperan penting dalam proses terjadinya karies. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokok. Metode: Penelitian deskriptif ini dilakukan dengan teknik pengambilan sampel consecutive sampling. Jumlah sampel yang diperoleh 20 orang yang terdiri dari 10 perokok kretek dan 10 bukan perokok. Bahan pemeriksaan berupa saliva diambil dari masing-masing sampel. Sampel saliva diencerkan, dieramkan dan diinokulasi pada media TYCSB. Sampel saliva kemudian diinkubasi pada 370C, secara fakultatif anaerob selama 48 jam. Koloni yang tumbuh dihitung menggunakan colony counter. Data dianalisis menggunakan t-test independen (α=0,05). Hasil: Rata-rata jumlah koloni Streptococcus mutans dari saliva perokok kretek sebesar 47,65 x 102 CFUs/ml sedangkan pada bukan perokok sebesar 11,1 x 102 CFUs/ml. Data statistik uji t independen mendapatkan nilai p<0,05 yang berarti terdapat perbedaan yang signifikan antara jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokok. Simpulan: Jumlah koloni Streptococcus mutans pada perokok kretek lebih tinggi dibandingkan bukan perokok.Kata kunci: Rokok kretek, bukan perokok, perokok kretek, Streptococcus mutans. ABSTRACTIntroduction: Kretek cigarettes are the most popular type of cigarette in Indonesia. Smoking can cause various oral health problems, one of them is caries. Streptococcus mutans is one of the essential bacteria in the process of caries. This study was aimed to determine the difference between the number of Streptococcus mutans colonies in kretek smokers and non-smokers. Methods: A descriptive study was carried out with the consecutive sampling technique. Samples obtained were as much as 20 people consisted of 10 kretek smokers and 10 non-smokers. Examination material was the saliva taken from each sample. The saliva were diluted, incubated, and inoculated on TYCSB media. Salivary samples were incubated at 37°C in facultative anaerob environment for 48 hours. The growing colonies were calculated using a colony counter. Data obtained were analysed using independent t-test (α = 0.05). Result: The average number of Streptococcus mutans colonies from kretek smoker saliva was 47.65 x 102 CFUs / ml, while in the non-smokers was 11.1 x 102 CFUs / ml. Independent t-test results were obtained the p-value < 0.05, which means that there was a significant difference between the number of Streptococcus mutans colonies in kretek smokers and non-smokers. Conclusion: The number of Streptococcus mutans colonies in kretek smokers saliva was higher than the non-smokers.Keywords: Kretek cigarettes, non-smokers, kretek smokers, Streptococcus mutans.

Diagnosis determination of chronic periapical abscess case using imaging radiography software and microbiological examination in female patients Diani Prisinda; Yuti Malinda; Yurika Ambar Lita; Sri Tjahajawati
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Diagnosis determination of chronic periapical abscess can be achieved by subjective, objective, and other examination such as radiographic and microbiological examination based on clinical finding and differences intensity at the periapical area with healthy bone suspected to be chronic abscess periapical. A microbiological examination performed to obtain the predominant bacteria that involved to determine an adequate medicament to succeed endodontic treatment. This study was aimed to compare intensity abscess lesion segmentation image reconstruction and surrounding bone for determine the diagnosis of periapical abscess more accurately and to determine the characteristic of bacteria colony from a periapical abscess tooth. Methods: The study sampling method was consecutive sampling (6 female) consisted of inclusion criteria patient with a periapical lesion in anterior maxillary that diagnosed based on clinical signs and symptoms. CBCT 3D scan of the maxillofacial region taken from of patients was measured image intensity periapical lesion and surrounding bone using semi-auto segmentation with ITK-SNAP 3.6.0. Samples from the root canals taken using three sequential sterile paper points. The samples were plated, and microorganisms were then isolated and identiﬁed by the colony characteristics. Results: There was a signiﬁcant difference between the mean content of lesion intensity of (n = 6,256.710 ± 81.930 and 636.022 ± 79.981 lesion boundary. The two-tailed p-value = 0.0050, this difference was considered to be very statistically significant. The mean of group lesion minus group surrounding bone equals to 163.715. 95% confidence interval of this difference from 75.542 to 251.888. Conclusions: Demineralisation of periapical bone can be determined by voxel intensity value in CBCT 3D radiograph using semi-auto segmentation ITK SNAP, could be recommended as a quantitative interpretation for detection periapical lesion and chronic periapical abscesses is a poly-microbial disease. Keywords: Chronic periapical abscess, endodontic, microbiology, radiograph CBCT

Effectiveness of ChKM solution compared to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess Yuti Malinda; Dea Hanin Azhara; Kartikaning Harnung; Diani Prisinda
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28642

Introduction: Chronic periapical abscesses are caused by polymicrobial bacteria, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis. These bacteria can be eliminated with chlorophenol camphor menthol (ChKM) solution and triple-antibiotic paste (TAP) as an intracanal medicament. This study compared the effectiveness of ChKM solution to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess. Methods: An experimental laboratory was conducted in-vitro with the microdilution method and optical density measurement using a spectrophotometer. The cell inhibition percentage was calculated to determine the MIC value. The MBC values were confirmed by cultivating the Mueller Hinton Agar samples, incubating them at 37°C for 24 hours, and observing bacterial growth. Bacteria did not grow in the medium at MBC value. The lower MIC and MBC were sensitive and could be an effective medicament choice. Results: The MIC ChKM solution inhibits E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277 were 4000, 4000, 2000, and 2000 µg/mL consecutively. While MIC of TAP were 6, 0.375, 0.75, and 1.5 µg/mL, respectively. Moreover, MBC of the ChKM solution were 32000, 32000, 8000, 8000 µg/mL and MBC of the TAP were 768, 24, 24, 96 µg/mL. Conclusions: ChKM solution and TAP effectively inhibited and killed E. faecalis, S. mutans, S. sanguinis, and P. gingivalis as an intracanal medicament, as seen from the MIC and MBC values. However, TAP is more effective than the ChKM solution because MIC and MBC values of TAP are much lower than the ChKM solution. This finding indicated that TAP is more effective at the lowest concentration than ChKM solution. It should be highlighted that this was an in-vitro study involving specific microbes; thus, further clinical research is needed.

Description of palatal rugae size and direction in children with gender difference Muhammad Arfianto Nur; Nina Djustiana; Yuti Malinda
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Indonesia has a high number of children victims of disaster in the last five years, but the children do not have identity cards, causing difficulties in the identification process. Palatal rugae are considered unique in individuals, and their morphology remains stable throughout life. Data of palatal rugae pattern in children aimed to facilitate the process of identification of the child's gender. The purpose of this research was to discover the description of palatal rugae in children which can be used as a reference of gender identification in children. Method: A descriptive research design was performed with purposive sampling method. Samples were taken from 119 maxilla images consisted of 69 images of girls maxilla and 50 images of boys maxilla. The study was performed by taking individual maxilla images with intraoral mirror and observing towards the direction of the palatal rugae according to the Carrea classification, and the size of palatal rugae according the Lysell classification. Results: The type IV direction and secondary size of palatal rugae were found to be dominant on both sides of maxilla in boys and girls. Conclusion: Palatal rugae in boys and girls were having the same direction which was the type IV, and the size of secondary rugae was found to be dominant on both sides of the palate. Palatal rugae also proofed to be able to become the complementary method for determining unidentified gender in children.

Human age estimation based on pulp volume of canines for chronological age estimation: Preliminary research Septian Rahmat Hidayat; Fahmi Oscandar; Yuti Malinda; Inne Suherna Sasmita; Murnisari Dardjan; Nani Murniati; Yurika Ambar Lita
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Age estimation has an important role in the process of human identification and needs to be performed in an efficient, fast, and accurate ways. Age estimation based on the pulp cavity volume of canines was referring to the theory stated that the apposition of secondary dentine during human life could be determined as an age indicator. This study was aimed to estimate the human age based on the pulp volume of canines for chronological estimation. Methods: Forty-one canine teeth from Cone Beam Computed Tomography (CBCT) 3D image sample from Dental Hospital of the Faculty of Dentistry Universitas Padjadjaran segmented into dental pulp and enamel were prepared for measurement of pulp volume using the ITK SNAP volumetric software. Descriptive analysis of pulp volume and regression equations were generated from regression analysis of pulp volume and tested for age estimation. Result: Dentine thickness was found to be increased from adolescence age range until adulthood age range. Logarithmic regression between pulp volume was statistically significant (p < 0,001). The coefficient of determination (R2) was 0.753 with a standard deviation of 8.421. Mathematics model for estimating age was: Age = 79.523 – (18.194 x ln(Pulp Volume)). The pulp cavity and dentine thickness were decreasing along the age. The same equation was also used in the same sample. There were several differences between chronological age and estimated age approximately 8.4 years following the regression result of R2 (75.3%). The rest of the sample (24.7%) have a big difference, and this could be affected by modifying variables such as occlusal force and human error measurement procedures. Conclusion: The human age can be estimated by the pulp volume of canines.Keywords: Age estimation, pulp volume, canine, ITK-SNAP, CBCT.

Acquaintance of bite mark identification procedures in Forensic Odontology Yuti Malinda; Dewi Zakiawati
Padjadjaran Journal of Dentistry Vol 27, No 3 (2015): November 2015
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Bite mark analysis casework strives to connect a biter to the teeth pattern present on the object linked in some way to crime or event. This analysis requiring an immediate response by the forensic odontologist since the marks fade rapidly in the living and the dead in a matter of hours. The aim of this article is to help the dentist to know and understand the procedures of bite mark identification in forensic odontology field. Literature review: Bite marks may be present the following situations, a fight between adults or children, a part of sexual or physical assault by adult on children, a rape, and homosexual activities. The marks can be single or multiple, varying degrees of severity from mild marking of the tissue to deep perforation, varying location may be found on breast, face/ head, abdomen, shoulder, upper extremity, buttocks, female genitalia, male genitalia, legs, ear, nose and neck. Discussion: Dentist should master the bite mark analysis procedures. When the suspect has been recognized, the first thing to do is swabbing the oral mucosa, and then taking photographs. The next steps are preparing to take impression, and always taking notes about the procedure and the result. A soon as all the documents are completed, do the matching procedures. Delay examination will cause the lost of valuable evidence. Conclution: Dentist should carefully determine the conclusion among these possibilities , the mark is “possible biter”, “probable biter”, or “with a high level of confidence, is the biter”

The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment Chany Mony Dwiayu Putri; Diani Prisinda; Yuti Malinda
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28638

Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.

Differences of enamel print patterns between buccal and lingual surfaces of extracted permanent maxillary first premolar Sheng Cheng Soo; Nani Murniati; Andriani Harsanti; Yuti Malinda; Fahmi Oscandar
Padjadjaran Journal of Dentistry Vol 32, No 2 (2020): July 2020
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no2.21639

Introduction: Ameloglyphics is the study of enamel print patterns on the tooth surface. Enamel print patterns are unique to each individual as they differ on each tooth. The similarity of tooth prints on buccal and lingual surfaces remains questionable as no research has been conducted thus far. The premolar was chosen in this experiment due to its low risk of damage because it is protected by cheek and rarely used compared to other teeth. Thus, the results obtained will be more precise and have higher accuracy than using other teeth. This research was aimed to investigate the differences between enamel print patterns on buccal and lingual surfaces of maxillary right first premolar. Methods: An analytic study was conducted by observing and analysing a total of 34 teeth samples using the purposive sampling method. The middle third of the buccal and lingual tooth surfaces was captured directly after magnified with a stereomicroscope. Photomicrographs of samples were then analysed using SourceAFIS biometric software. This software will process the image and shows patterns of enamel print. Afterwards, the enamel print patterns were observed and classified according to Manjunath Classification. Results: The results suggested that all 34 samples show a similarity of enamel print patterns on both the buccal and lingual surfaces. The interpretations of photomicrographs were statistically analysed using the Statistical Package of Social Science (SPSS) software. The similarity may be due to the same placement of developing tooth bud, temperature, environmental factor, genetics, pressure and nutrition to the ameloblasts cells in a tooth. Conclusion: Both buccal and lingual in the samples show high similarity due to the very high frequencies of similar sub-patterns occur on both surfaces. Tooth print can be used as a valuable tool in forensic science for personal identification with good prospects in the field of forensic dentistry.

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