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Journal : Jurnal Kesehatan Manarang

 1 
Identifikasi Jenis Bakteri pada Luka Kaki Diabetik berdasarkan Penyebab Luka di Rumah Perawatan Luka dan Poliklinik Luka di Kota Makassar Nurwahidah, Nurwahidah; Yusuf, Saldy; Tahir, Takdir
Jurnal Kesehatan Manarang Vol 4 No 2 (2018): Desember 2018
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.934 KB) | DOI: 10.33490/jkm.v4i2.70

Abstract

The high incidence of Diabetes Mellitus (DM) causes increased complications associated with DM. One is the Diabetic Foot Ulcer (DFU). Delayed healing in DFU is usually caused by infection. The infection of DFU contains many gram negative (-) and gram positive (+) bacteria, so it is important. To identify the type of bacteria present on the DFU based on the cause. This was an experimental cross sectional study conducted at ETN Center, Griya Afiat, Avaro, Isam Cahaya,  RSUP DR. Wahidin Sudirohusodo Makassar and Microbiology Laboratory Hospital Unhas Makassar. There were 29 respondents who met the inclusion criteria. Specimens of wound fluid were taken by swabbing technique. The specimen was inoculated on NA (Nutrient Agar) and MCA (MacConcey Agar) then incubated at 37 ° C for ± 18-24 hours to see bacterial morphology. If a gram negative bacteria (-) is tested biochemically by bacteria is grown on TSI (Tripple Sugar Iron), SIM (Indole Motility Sulfide), citrate urea, kovacks. As for gram positive bacteria (+) in MSA medium (Manitol Salt Agar), catalase (H2O2), incubated for 18-24 hours at 37 ° C, record the results of identification and match the bacterial identification table based on gram properties. Based on morphological test, 29,5% gram negative (-) and Gram positive (+) bacteria were 8.8% in traumatic LKD, with the dominant gram negative (-) species being proteus vulgaris (8.8%) and staphylococcus aureus 8.8%) in the gram bacteria (+). While for non trauma LKD found gram negative bacteria (-) as much as 58.8% and gram positive bacteria (+) 2.9% with the dominant type of bacteria are S.Aureus (2.9%) and P. Mirabilis (17.6%) for gram negative bacteria (-). Identification of bacteria in DFU caused by trauma and non trauma is dominant gram negative bacteria (-). Further research is expected to identify the type of bacteria based on the Wagner and sensitivity of the bacteria.
Co-Authors Abdul Rahman Abu Bakar Ahmad Ahmad Ahmad Harjono akbar, nurhidayah Alimuddin, Nurkhaerat Andi Mariani, Andi Angga, Prayogi Dwina Ansar Ansar Arismansyah, Arismansyah Astria, Fitri Puji Budiman Budiman Darmiany Djabier, Abdoellah Eva Syarifatul Jamilah Fadillah, Diyah Fadillah, Putri Khusnul Fauzi Ahmad Muda Gomes, Aprilia Nelina Haidah, Nur Harmayetty, Harmayetty Hasan, Asriani Hidayat Hidayat Ibrahim Ibrahim Irawan, Heri Jamali Jamali, Jamali Karma, I Nyoman Kurniati MA, Ratnah Makki, Muhammad Melvi Lesmana Alim, Melvi Lesmana Muhammad Suyudi Muslimin, Abdul Azis Nabir, Abdul Muhaemin Nildawati, Nildawati Nongkengr, Hasan Nur, Ismail M. Nursalam Nursalam Oktaviana, Putri Ramadhani, Alifia Ranti Ekasari, Ranti Rini Hendari, Rini Rizandhi, Andhi Saldy Yusuf Sani, Chaerul Sari, Mega Puspita Sigit Sugiarto Siti Istiningsih, Siti Sofian, Sofian Sopialena . Sulsana, Raih Mitra Suranti, Ni Made Yeni Suryadi, Andi Susila, Wahyu Dini Candra Syaiful . Syamsiah, Sitti Takdir Tahir, Takdir Tona, Tasdik Wahyu Dini Metrikayanto, Wahyu Dini Wida, Eka Kusniatul