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Ida Nurwati
Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Majalah Kedokteran Bandung

 1 
PENURUNAN KADAR PRO-ADRENOMEDULLIN MENCIT BALB/C MODEL SEPSIS DENGAN KORTIKOSTEROID DOSIS RENDAH Prasetyo, Diding Heri; Nurwati, Ida; Indrayanto, Yoseph; Hermawan, A. Guntur
Majalah Kedokteran Bandung Vol 46, No 2 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1438.35 KB)

Abstract

Penggunaan kortikosteroid dosis rendah pada penatalaksanaan sepsis tahap awal masih diperdebatkan. Pemberian kortikosteroid dosis rendah pada sepsis dapat mengurangi respons inflamasi sitemik, menghambat produksi sitokin proinflamasi dan mediator inflamasi, serta menurunkan adhesi leukosit ke endotel. Pro-adrenomedullin (pro-ADM) merupakan biomarker keparahan sepsis. Penelitian ini bertujuan untuk menganalisis efek penggunaan kortikosteroid dosis rendah pada kadar pro-ADM serum pada mencit Balb/C model sepsis tahap awal. Penelitian ini merupakan penelitian eksperimental laboratoris, dengan sampel 30 ekor mencit Balb/C jantan yang dibagi menjadi kelompok kontrol, sepsis, dan sepsis+kortikosteroid dosis rendah. Penelitian dilakukan di Laboratorium Histologi dan Biomedik, Fakultas Kedokteran, Universitas Sebelas Maret Surakarta, selama periode April?September 2012. Untuk membuat model sepsis, hewan coba diinokulasi lipopolisakarisa/LPS (E. coli) dosis 0,1 mg/mencit secara intraperitoneal (i.p). Kelompok tikus kontrol tidak diinokulasi selama penelitian. Kortikosteroid dosis rendah yang digunakan adalah metil prednisolon dosis 0,05 mg/mencit/12 jam secara i.p. Mencit kontrol tidak diinokulasi selama penelitian. Kadar pro-ADM serum ditentukan menggunakan enzyme-linked immunosorbent assay (ELISA). Uji kadar pro-ADM serum menggunakan analysis of variance (ANOVA) dan untuk menentukan perbedaan kemaknaan digunakan p<0,05. Kortikosteroid dosis rendah secara bermakna menurunkan kadar pro-ADM dibandingkan dengan kelompok sepsis (33,0±2,9 pg/mL vs 48,5±6,1 pg/mL; p<0,001). Simpulan, penggunaan kortikosteroid dosis rendah menurunkan kadar pro-ADM pada hewan coba model sepsis tahap awal. [MKB. 2014;46(2):68?72]Kata kunci: Kortikosteroid dosis rendah, pro-adrenomedullin, sepsisPro-adrenomedullin Level Reduction in Balb/C Sepsis Model Mice through the Use of Low-Dose CorticosteroidThe use of low-dose corticosteroids in the management of early stage of sepsis is still debated. Low-dose corticosteroids in sepsis can reduce the systemic inflammatory response, inhibit the production of pro-inflammatory cytokine, inflammatory mediators, and decrease adhesion of leukocytes to the endothelium. Pro-adrenomedullin (pro-ADM) is a biomarker of sepsis severity. This study aimed to analyze the effects of the use of low-dose corticosteroids on the levels of pro-ADM in the serum in Balb/C mice model of sepsis at the early stage. This study was an experimental research laboratory, with 30 male Balb/C mice which were divided into control, sepsis, and sepsis+low-dose corticosteroids groups. The study was conducted at the Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, during the period of April to September 2012. Sepsis was induced in the male Balb/C mice by inoculation through an intraperitoneally (i.p) injection of lipopolysaccharide/LPS (E. coli) with 0.1 mg/mice/i.p. dose for sepsis mice model. Control mice were not inoculated during the study. Low-dose corticosteroids used was methyl prednisolone at a dose of 0.05 mg/mice/12 hours/i.p. Pro-ADM serum level was measured with enzyme-linked immunosorbent assay (ELISA). One way analysis of variance (ANOVA) for pro-ADM serum level and p<0.05 were used to determine the significant differences. Low-dose corticosteroids significantly decreased pro-ADM level (33.0±2.9 pg/mL vs 48.5±6.1 pg/mL, p<0.001) compared to the sepsis group. In conclusion, the use of low-dose corticosteroids reduce levels of pro-ADM in the early stages of sepsis. [MKB. 2014;46(2):68?72]Key words: Low-dose corticosteroids, pro-adrenomedullin, sepsis DOI: 10.15395/mkb.v46n2.276
Hepatoprotective and Antifibrotic Effects of Indonesian Propolis Diding Heri Prasetyo; Sarsono Sarsono; Ida Nurwati; Prihandjojo Andri Putranto; Martini Martini; Nabila Aushaf Prasetyo
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.973 KB) | DOI: 10.15395/mkb.v51n3.1768

Abstract

Liver cirrhosis is the irreversible stage in liver damage process which occurs after liver fibrosis due to necro-inflammatory activities and liver fibrosis. Therefore, inhibition of liver inflammation and fibrosis is very important to prevent liver cirrhosis. This study aimed to analyze the effect of ethanol extract of propolis (EEP) from mount Lawu, Indonesia to prevent liver damage and fibrosis progression in mice with hepatic cirrhosis. This study was performed during the period of June 2018 to May 2019 on a sample of 32 male Balb/C mice divided into control group (P1), induction of carbon tetrachloride (CCl4 ) group (P2), induction of 50 mg/BW CCl4 + EEP group (P3), and (induction of 100 mg/KgBW CCl4 + EEP (P4) with each group consisted of eight mice. The CCl4 in olive oil was administered intraperitoneally three times a week for six weeks. Mean differences between group was determined using ANOVA test with a significance level of 0.05. The induction of CCl4 increased liver cell damage and serum alanin aminotransferase (ALT) level. However, the addition of EEP significantly (p<0.001) reduced liver cell damage as seen in P3 (54.38±4.17 per 100 liver cells) and P4 (37.13±4.36 per 100 liver cells) groups and serum alanin aminotransferase (ALT) as seen in P3 (291.19±113.92 U/L) and P4 (229.38±73.45 U/L) groups. The APRI scores were also reduced after EEP as seen in P3 (0.738±0.292) and P4 (0.513±0.253) groups. Thus, EEP isolates from Gunung Lawu can reduce liver cell damage and fibrosis in mice model of hepatic cirrhosis. Efek Hepatoprotektif dan Antifibrotik Propolis IndonesiaSirosis hati (SH) merupakan tahap ireversibel dalam proses kerusakan hati yang terjadi setelah fibrosis hati sebagai hasil aktivitas nekroinflamasi dan fibrosis hati. Oleh karena itu, penghambatan inflamasi hati dan fibrosis sangat penting untuk mencegah terjadi SH. Penelitian ini bertujuan menganalisis efek ekstrak etanol isolat propolis (EEP) gunung Lawu dalam mencegah progresifitas kerusakan dan fibrosis hati pada mencit model SH. Penelitian dilakukan di Laboratorium Histolgis Fakultas Kedokteran Universitas Sebelas Maret Surakarta dan Lembaga Penelitian dan Pengujian Terpadu (LPPT) Universitas Gajah Mada Yogyakarta . Penilitian ini dilakukan periode Juni 2018 sampai Mei 2019, dengan sampel 32 ekor mencit Balb/C jantan dibagi menjadi kelompok (P1) kontrol negatif, (P2) induksi karbon tetraklorida (CCl4 ), (P3) induksi CCl4 +EEP dosis 50 mg.kgBB-1, dan (P4) induksi CCl4 +EEP dosis 100 mg.kgBB-1, dengan tiap-tiap sampel terdiri dari delapan ekor mencit Balb/C jantan. CCl4 dalam minyak zaitun diberikan pada tikus Balb/C tiga kali seminggu selama enam minggu secara intraperitoneal. Uji ANOVA digunakan untuk menentukan perbedaan rata-rata antar kelompok dengan tingkat kemaknaan sebesar 0,05. Namun, penambahan EEP secara signifikan (p<0,001) menurunkan tingkat kerusakan sel hati seperti yang terlihat pada P3 (54,38±4,17 per 100 sel hati) dan kelompok P4 (37,13±4,36 per 100 sel hati) dan serum alanin aminotransferase (ALT) seperti yang terlihat dalam kelompok P3 (291,19±113,92 U / L) dan P4 (229,38±73,45 U / L). Hasil skor APRI menunjukkan kelompok P1(0,213±0,113) dan P2 (0,863±0,534), EEP dapat menurunkan skor APRI pada kelompok P3 (0,738±0,292) dan P4 (0,513±0,253). Simpulan, EEP isolat Gunung Lawu menurunkan tingkat kerusakan sel hati dan fibrosis pada mencit model SH.
Co-Authors A. Guntur Hermawan Betty Saptiwi Betty Saptiwi Betty Saptiwi Danus Hermawan Danus Hermawan Dicky Maulana Lazuardi Diding H P Diding Heri Prasetyo Diding Heri Prasetyo Dyah Ratna Budiani Dyah Ratna Budiani Dyah Ratna Budiani Eriana Melinawati Eric Edwin Yuliantara Fahmi, Muhammad Izzul Widad Gresmita Rindi Winarti Hadi, Cahyono Hermawan, Danus Ika Tristanti Jarot Subandono Jarot Subandono Jarot Subandono Lazuardi, Dicky Maulana Martini Martini Martini Martini Martini Martini Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Mutmainah Mutmainah Nabila Aushaf Prasetyo Nanang Wiyono Noni Kartika Sari Nur Hafidha Hikmayani Nur Hafidha Hikmayani Nutria Widya Purna Anggraini Octavia, Dhamayanti Eka Prihandjojo Andri Putranto Risya Cilmiaty A. R. Risya Cilmiaty AR Risya Cilmiaty, Risya Sari, Noni Kartika Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Selfi Handayani Selfi Handayani Selfi Handayani Selfi Handayani Sigit Setyawan Siti Marufah Sri Hartati Hadinoto Sri Haryati Sri Sulistyowati Uki Retno Budihastuti Wesliaprilius, Todung Antony Wiyono, Nanang yoseph Indrayanto Yulia Sari Yulyanti, Yulyanti