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All Journal Prosiding Seminar Biologi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri Kultivasi Proceeding Biology Education Conference
Ika Roostika
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : Kultivasi

 1 
Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP Rahayu, Suci; Saptadi, Darmawan; Azmi, Chotimatul; Kusumanegara, Kusumawaty; Handayani, Tri; Roostika, Ika; Bermawie, Nurliani; Maulana, Haris
Kultivasi Vol 22, No 3 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i3.50581

Abstract

Centella's need for industrial raw materials is high as a medicinal plant. These needs can be met through rapid multiplication using tissue culture techniques. In this study, induction and proliferation for a callus of centella cv. Castina 3 was conducted in the MS basal medium plus 4 mgL-1 Dicamba withand enriched with 7 concentrations of BAP (0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.1 mgL-1). Two kinds of explant were used, i.e., leaf and petiole. The results revealed that the addition of BAP in MS plus Dicamba medim stimulated better and produced a higher callus growth rate, both from leaf and petiole explants, than that media with Dicamba alone. Furthermore, 4 mgL-1 Dicamba + 1.1 mgL-1 BAP had a friable callus in the induction phase and a friable-compact callus in the proliferation phase. From this finding, it can be considered to use a combination of 4 mgL-1 Dicamba with 1.1 mgL-1 BAP in callus induction and proliferation for Centella rapid multiplication. 
Co-Authors Chotimatul Azmi, Chotimatul Darmawan Saptadi, Darmawan Deden Sukmadjaja Efendi, Darda Fitri Damayanti GA Wattimena Gustaaf A Wattimena, Gustaaf A Gustaaf Adolf Wattimena Ika Mariska Ireng Darwati Kusumanegara, Kusumawaty Maulana, Haris N Khumaida N Sunarlim Nurliani Bermawie Nurul Khumaida RAGAPADMI PURNAMANINGSIH RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Rara Puspita Dewi Lima Wati, Rara Puspita Dewi Lima RITA MEGIA Samsurianto Samsurianto Suci Rahayu Tri Handayani V N Arief Y Supriati Yudiwanti Yudiwanti