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Yeni Dhamayanti
Departemen Anatomi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga, Kampus C Mulyorejo, Surabaya, Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Pengaruh Pemaparan Logam Berat Pb (Timbal) Terhadap Perubahan Warna dan Peningkatan Persentase Anakan Jantan Daphnia spp. [Effect Of Heavy Metal Pb (Lead) Expossure To The Color Change and Increase The Percentage Of Male Offsprings Of Daphnia spp. ] A. Shofy Mubarak,; Akhsan Panna; Yeni Dhamayanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.583 KB) | DOI: 10.20473/jipk.v1i2.11686

Abstract

AbstractPb (lead) was a metal that does'nt regulated by water organisms so that the metal was constantly acumulated in organisms tissue. Currently, the pollution Pb in the waters of Indonesia have exceeded the maximum threshold of pollution defined by the government.Daphnia spp. is the organism that have been developed as a bioassay in some developed countries like U.S. and Japan because it has a rapid life cycle that is about three weeks, are sensitive to the chemical in the waters ecology and has an important role in the ecology of freshwater as the first of trophic level in the waters ecology. Target of this research is to obtain information about the concentration of heavy metals Pb that correlated with of Daphnia spp. color changes and increase of male offsprings of Daphnia spp.. This research was th thconducted on november 24 , 2008 until December 8 , 2008 in the Laboratory Education of fisheries, Faculty of Fisheries and Marine, Airlangga University. This research use method of experimental design with Completely Randomized Design (RAL)  with five treatments and four rerplicates. Treatment A (control), exposure of Pb in concentration 0 mg/L. Treatment B, Pb concentration 0.90 mg/L, Treatment C, Pb concentration 1.8 mg/L, Treatment D, Pb concentration 2.7 mg/L, and Treatment E with Pb concentration 3.63 mg/L. Collected data were analyzed using Analysis of Variance (ANOVA) with error rate or á = 0,05, if there are a different, hence continued with Duncan's Multiple Range Test. Collected data were analyzed again using linear regression graph to estimate the enhanced in every treatment. The results of research indicates that the heavy metal Pb exposure with different concentrations to the adult female Daphnia spp. did not showing significant different of the daphnia spp. color change. This is because the condition of Hypoxia (oxygen deficiency) more dominant than the increase of methyl farnesoate to influence increase of Daphnia spp.'s haemoglobin synthesa. Besides, research also indicates that the heavy metals Pb exposure with different concentrations to the adult sex Daphnia spp. provide a very significant percentage of male Daphnia spp. offsprings. This happens due to the increase of methyl farnesoate in Daphnia spp. inhibit the formation of female sex of Daphnia spp. offsprings by adult Daphnia spp. that expossured by Pb. Result of processing data using linear regression is a formula y = 18.76x + 3.104. this formula can be used to estimate concentration of Pb in water based on percentage of male offsprings of Daphnia spp. Water quality during research showed the waters pH range between 8.2 - 8.5,  dissolved oxygen (DO) oranged between 8.0 - 8.5 mg/L, waters temperature was 26 C and ammonia level was 0.03 mg/L. This Conditions of water quality was the optimal conditions to support Daphnia spp. life. 
Hindlimb Osteometry of Lohmann Broiler on 7, 21 and 35 days Ulfiani Fauzia Hanafi; Yeni Dhamayanti; Muhammad Thohawi Elziyad Purnama; Soeharsono Soeharsono; Prima Ayu Wibawati; Ragil Angga Prastiya
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.23-36

Abstract

Patterns of growth can be determined one of them through quantitative measurements, namely osteometry. The aim of this study was to determine the growth of patterns of Lohmann broiler chickens based on hindlimb osteometry at the age of 7, 21 and 35 days. This study used 30 Lohmann broiler chickens aged 7, 21 and 35 days. Osteometry techniques are performed on all the bones of hindlimb with variable Greatest length (GL), Breadth of the proximal end (Bp), Breadth of the distal end (Dd), Minimum breadth of diaphysis (Sd), Circular of the proximal end (Cp), Circular of corpus (Cc), and Circular of distal end (Cd). The data obtained is recorded in units of centimeters (cm). Data of body weight and weight of each bone are also recorded in grams (g). Data were analyzed by ANOVA test, if the result obtained with a significance of 5% then the test continued with Pos Hoc Duncan. The results showed that from 6 osteometry variables, body weight, and bone mass obtained significant differences (p<0.05). The fastest growth rate is reached when Lohmann broiler chickens are 21 days old.
Pola Pertumbuhan Ayam Broiler Strain Lohmann Berdasarkan Osteometri Tulang Sayap Neneng Ismi Megawati; Yeni Dhamayanti; Muhammad Thohawi Elziyad Purnama; Soeharsono Soeharsono; Aditya Yudhana; Maya Nurwartanti Yunita
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.216-223

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pola pertumbuhan dan osteometri serta untuk mengetahui titik osteometri tulang sayap ayam broiler strain Lohmann. Pengukuran tulang sayap dilakukan dengan mengukur panjang, lebar, keliling dan barat tulang dengan menggunakan jangka sorong dengan ketelitian 0,05 mm. Hasil penelitian pola pertumbuhan ayam broiler strain Lohmann tulang sayap pada umur 7, 21 dan 35 hari, diperoleh  hasil yang signifikan  (p<0.05) terhadap nilai rata-rata panjang, lebar (proximal, corpus dan distal), keliling (proximal, corpus dan distal) dan berat pada os scapula, os coracoid dan os clavicula dan tulang-tulang alat penyusun gerak bebas yang terdiri dari os humerus, os radius, os ulna, os metacarpal I, os metacarpal II, os metacarpal III, ossa digiti I, ossa digiti II dexter phalang I dan Phalang II, ossa digiti II sinister Phalang I dan Phalang II  serta os digiti III. Data di analisis menggunakan Analysis of Variance (ANOVA) selanjutnya diuji menggunakan uji Post Hoc Duncan. Ayam broiler strain Lohmann memiliki titik pertumbuhan maksimum saat berumur 21 hari yang ditunjukkan dengan terlihat ada titik optimal dan di lanjutkan dengan pertumbuhan yang melambat. Pada titik ini memicu pembentukan daging pada ayam broiler strain Lohmann sebelum ayam mencapai batas pertumbuhan.
Toxic Effect of Robusta Coffee Extract (Coffea canephora) on Bali Cattle Spermatozoa After Equilibration Adam Fahmi Fiqih; Soeharsono Soeharsono; Hani Plumeriastuti; Maya Nurwartanti Yunita; Yeni Dhamayanti; Aditya Yudhana
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.189-192

Abstract

The aim of this study was examine the effect of robusta coffee in bali cattle semen. A total of six cattles cement were given four treatments each P0 without adding Robusta coffee extract, P1 was given an additional 5%, P2 10%, and P3 15%. The results are observed by assessing motility, viability, and abnormalities. The data obtained were analyzed by Analysis of Variance (ANOVA) and continued with the duncan. Based on the analysis of variance in obtaining the results of the study, it was shown motility of P0 was significantly different from P1, P2, and P3 (p<0.05). Viability of P0 (control) different significantly from P1 , P2, and P3 (p<0.05). P3 motility different significantly from P0, P1, and P2 (p<0.05). Addition of robusta coffee extract of 10% and 15% decreased spermatozoa quality.
The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi Indah Puspita Ningrum; Soeharsono Soeharsono; Prima Ayu Wibawati; Yeni Dhamayanti; Maya Nurwartanti Yunita
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.48-53

Abstract

Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Milla Nursadida; Hani Plumeriastuti; Yeni Dhamayanti; Widjiati Widjiati; Epy Muhammad Luqman; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
GAMBARAN PATOLOGI HEPAR IKAN LELE DUMBO (Clarias gariepinus) YANG DIPAPAR LOGAM TIMBAL NITRAT Pb(NO3)2 Yolanda Dellavia; Thomas Valentinus Widiyatno; Boedi Setiawan; Arimbi Arimbi; Bambang Poernomo; Yeni Dhamayanti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21092

Abstract

This research aimed to know the changed damage of liver African catfish (Clarias gariepinus) were exposed by lead nitrat. This study used twenty four of African catfish (Clarias gariepinus) with average weight of 20-25 grams, size 10-12 cm, age ± two months. This research was designed by a completely randomized design (CRD). All member of population of the African catfish were divided into six groups, consist of six repetitions each, namely P1, P2, P3, and P0 as a negative control respectively.  P1 was given with dosage of lead nitrat 7,26 mg/liter, P2 was given with dosage of lead nitrat 14,53 mg/liter, and P3 was given with dosage of lead nitrat 29,06 mg/liter. According to the macroscopic observed, show that liver became swollen and pale.  The histopathological features of hepar were examined under light microscope in 400 times magnification. Scoring method were using Bernet Scoring Method to examined the presence of degeneretion, congesti, necrotic, and infiltration of leukocyte. Then, Kruskal-Wallis test through with Mann-Whitney test of statistical analysis. The statistical analysis showed the median number of P0 (0,05), P1 (0,45), P2 (1,10), and P3 (1,40) respectively. From the result can be concluded lead exposure with dose 29,06 mg/liter severe which was heavily histopatological in hepatocytes cell of african catfish (Clarias gariepinus) liver.
PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Edi Purnomo; Epy Muhammad Luqman; Hermin Ratnani; Hani Plumeriastuti; Maslichah Mafruchati; Yeni Dhamayanti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.
Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan Febriyono Anggela Prakoso; Raden Tatang Santanu Adikara; Kusnoto Kusnoto; Sri Hidanah; Yeni Dhamayanti; Sunaryo Hadi Warsito
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28586

Abstract

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.
Co-Authors A. Shofy Mubarak Adam Fahmi Fiqih Aditya Yudhana Akhsan Panna Arimbi Arimbi Bambang Poernomo Boedi Setiawan Edi Purnomo Epy Muhammad Luqman Febriyono Anggela Prakoso Hani Plumeriastuti Hermin Ratnani Indah Puspita Ningrum Kusnoto Kusnoto Maslichah Mafruchati Maya Nurwartanti Yunita Milla Nursadida Muhammad Thohawi Elziyad Purnama Neneng Ismi Megawati Prima Ayu Wibawati Ragil Angga Prastiya Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Tatang Santanu Adikara Thomas Valentinus Widiyatno Ulfiani Fauzia Hanafi Warsito, Sunaryo Hadi Widjiati Widjiati Yolanda Dellavia