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Satrijo Saloko
Faculty Of Food Technology And Agro Industry, University Of Mataram, Nusa Tenggara 83126 Indonesia
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Journal : Journal of Food and Pharmaceutical Science

 1 
α-Amilase Inhibitory Activity of Fraction of Ethanolic Extract of Lebui Seed (Cajanus cajan (L.) Millsp.) Grown in West Nusa Tenggara Dian Resti Setyaningrum; Rumiyati; Yekti Asih Purwestri; Satrijo Saloko; Agung Endro Nugroho; Yudi Pranoto; Sri Widyastuti
Journal of Food and Pharmaceutical Sciences Vol 8, No 2 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.720

Abstract

Diabetes is a metabolic syndrome characterized by high blood sugar levels or hyperglycemia.Alpha-amylase inhibitors can be used as an antidiabetic that controls postprandial hyperglycemia.Alpha-amylase inhibitors are found in plants as protein and non-protein bioactive content such as in lebuiseed. The purpose of this study was to determine the activity of alpha-amylase inhibition of the fraction ofethanol extract of lebui seed. This research was carried out by extraction of lebui seed and then fractionatedlebui extract to obtain n-hexane fraction (HF), ethyl acetate fraction (EAF), and water fraction (WF). Thefractions then tested to alpha-amylase inhibition activity in vitro by measuring the reducing sugar using3.5-dinitrosalicylic (DNS) reagent. Furthermore, the identification of the bioactive content of each fraction wascarried out using thin layer chromatography. The results of alpha-amylase inhibition activity test showed thatall fractions had the ability to inhibit alpha-amylase. The IC50 value of the n-hexane fraction, ethyl acetatefraction, and water fraction were 173.05 mg/ml, 9.98 mg/ml, and 137.19 mg/ml, respectively. Ethyl acetatefraction has the smallest IC50 value compared to other fractions. Based on the identification of the bioactivecontent, bioactive content that is only found in the ethyl acetate fraction but not in other fractions are tannins.Therefore, the chemical content in lebui seed which acts as an alpha-amylase inhibitor might be tannins.
Co-Authors Agung Endro Nugroho Agus Purbathin Hadi Ahmad Alamsyah Ahmad Alamsyah Alfian Pujian Hadi Aluh Nikmatullah Baiq Nisrina Nurubay Baiq Nuzulina Bambang Budi Santoso Bambang Setiaji Cicilia, Siska Dian Resti Setyaningrum Dody Handito, Dody Dwiani, Afe Eva Mayasari Hartina Hartina L. Mukhtar Atmawinata M. Qazuini Maria Ulfa Muhammad Nursan Muhammad Sarjan Mutia Devi Ariyana Oke Anandika Lestari Purnama Darmadji rekapangan, Nazaruddin Rizka Amalia Rumiyati Sahrul Alim Siska Cicilia Siska Cicillia Siti Rakmah Sopiandi Sopiandi Sri Widyastuti Sri Widyastuti Yekti Asih Purwestri Yudi Pranoto