Garuda - Garba Rujukan Digital

The Effectiveness of Using Honey in Feed for Fish Sex Change Sword Plate (Xiphophorus helleri) Mustaqim Mustaqim; Wenny Novita Sari; Jumadi
SEAS (Sustainable Environment Agricultural Science) Vol. 7 No. 1 (2023)
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/seas.7.1.5858.01-08

This study aims to determine the effect of forest bee honey on changes in male sex of sword platy fish (Xiphophorus helleri). The benefits of this study were to determine the appropriate dose of honey for changes in male sex and how the survival rate of sword platy fish larvae was. This study was conducted using a completely randomized design (CRD) with five treatments and three replications, namely Treatment A: Control (Ordinary Feed), Treatment B: Dosage of 3 ml of honey mixed into 100 g of feed, Treatment C: Dosage of 4 ml of honey which is mixed into 100 g of feed, Treatment D : Dosage of 5 ml of honey mixed into 100 g of feed, Treatment E : Dose of 6 ml of honey mixed into 100 g of feed. The results showed that mixing honey into feed gave a significantly different effect on the percentage of male sex ratio where Fcount > Ftable (5.15 > 3.48). Where the best dose is found in treatment E: 6 ml of honey mixed into 100 g of feed with a percentage of 66.05%. While the lowest percentage value is in treatment A (Control) with a total percentage of 40.79%. For the survival rate, mixing honey into the feed had no effect between all treatments. The percentage of survival rates is still in the range above 50%. The range of water quality values is the temperature ranges from 26.55-27.52 0C, pH ranges from 7.02-7.06 and dissolved oxygen ranges from 5.13-5.31 ppm.

KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika Safika; Wenny Novita Sari; Gressha Vionalle Ademi; Ulfi Widi Arsih; Darmawi Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.

PERSENTASE BERAHI DAN KEBUNTINGAN KAMBING PERANAKAN ETTAWA (PE) SETELAH PEMBERIAN BEBERAPA HORMON PROSTAGLANDIN KOMERSIAL Hafizuddin Hafizuddin; Wenny Novita Sari; Tongku Nizwan Siregar; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui perbandingan efektivitas pemberian hormon prostaglandin komersial yang berbeda terhadap persentase berahi dan kebuntingan kambing peranakan ettawa. Kambing betina yang digunakan mempunyai kriteria umur 2,5-3,5 tahun, sehat, tidak bunting, minimal 2 bulan pasca partus, sudah pernah beranak, dan mempunyai bobot badan yang relatif sama. Di samping itu digunakan 2 ekor kambing jantan untuk membantu deteksi berahi. Hewan percobaan dibagi atas 3 kelompok perlakuan, masing-masing kelompok terdiri atas 5 ekor kambing. Kelompok perlakuan I diinjeksi dengan 2,5 ml Lutalyse (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%), kelompok perlakuan II diinjeksi dengan 0,5 ml Prostavet (etiproston 5 mg/2 ml dan ethylen dioxy 15 mg/2 ml), dan kelompok perlakuan III diinjeksi dengan 1,5 ml Capriglandin (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml). Penyuntikan dilakukan 2 kali secara intramuskuler dengan interval 10 hari setelah penyuntikan pertama. Kambing-kambing yang memperlihatkan gejala berahi dikawinkan secara inseminasi buatan. Diagnosis kebuntingan dilakukan dengan menggunakan USG 30 hari setelah inseminasi. Data onset berahi dianalisis menggunakan analisis varian, sedangkan persentase berahi dan kebuntingan dianalisis secara deskriptif. Onset berahi ketiga kelompok perlakuan masing-masing adalah 40,8±0,57 36,0±0,57 dan 50,4±1,52 jam (P0,05). Persentase berahi pada ketiga kelompok adalah sama yakni 100%, sedangkan persentase kebuntingan pada kelompok I, II, dan III masing-masing adalah 100, 80, dan 60%. Dari hasil penelitian dapat disimpulkan bahwa onset dan persentase berahi tidak dipengaruhi oleh ketiga prostaglandin komersial yang berbeda tetapi berpengaruh terhadap persentase kebuntingan kambing PE.

2. Characterization Of Lactic Acid Bacteria (LAB) Origin Sumatran Orangutan (Pongo abelii) In Zoo Bukittinggi Based On Analysis 16 S rRNA Siti Hajar; Safika Safika; Darmawi Darmawi; Wenny Novita Sari; Erdiansyah Rahmi; Yandi Syahputra; Nurliana Nurliana; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.

KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika Safika; Wenny Novita Sari; Gressha Vionalle Ademi; Ulfi Widi Arsih; Darmawi Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16426

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.

PERSENTASE BERAHI DAN KEBUNTINGAN KAMBING PERANAKAN ETTAWA (PE) SETELAH PEMBERIAN BEBERAPA HORMON PROSTAGLANDIN KOMERSIAL Hafizuddin Hafizuddin; Wenny Novita Sari; Tongku Nizwan Siregar; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.366

Penelitian ini bertujuan mengetahui perbandingan efektivitas pemberian hormon prostaglandin komersial yang berbeda terhadap persentase berahi dan kebuntingan kambing peranakan ettawa. Kambing betina yang digunakan mempunyai kriteria umur 2,5-3,5 tahun, sehat, tidak bunting, minimal 2 bulan pasca partus, sudah pernah beranak, dan mempunyai bobot badan yang relatif sama. Di samping itu digunakan 2 ekor kambing jantan untuk membantu deteksi berahi. Hewan percobaan dibagi atas 3 kelompok perlakuan, masing-masing kelompok terdiri atas 5 ekor kambing. Kelompok perlakuan I diinjeksi dengan 2,5 ml Lutalyse (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%), kelompok perlakuan II diinjeksi dengan 0,5 ml Prostavet (etiproston 5 mg/2 ml dan ethylen dioxy 15 mg/2 ml), dan kelompok perlakuan III diinjeksi dengan 1,5 ml Capriglandin (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml). Penyuntikan dilakukan 2 kali secara intramuskuler dengan interval 10 hari setelah penyuntikan pertama. Kambing-kambing yang memperlihatkan gejala berahi dikawinkan secara inseminasi buatan. Diagnosis kebuntingan dilakukan dengan menggunakan USG 30 hari setelah inseminasi. Data onset berahi dianalisis menggunakan analisis varian, sedangkan persentase berahi dan kebuntingan dianalisis secara deskriptif. Onset berahi ketiga kelompok perlakuan masing-masing adalah 40,8±0,57 36,0±0,57 dan 50,4±1,52 jam (P0,05). Persentase berahi pada ketiga kelompok adalah sama yakni 100%, sedangkan persentase kebuntingan pada kelompok I, II, dan III masing-masing adalah 100, 80, dan 60%. Dari hasil penelitian dapat disimpulkan bahwa onset dan persentase berahi tidak dipengaruhi oleh ketiga prostaglandin komersial yang berbeda tetapi berpengaruh terhadap persentase kebuntingan kambing PE.

Effectiveness of aquatic plants as phytoremediation in palm oil processing factory wastewater Mustaqim Mustaqim; Wenny Novita Sari; Irfannur Irfannur; Yusrizal Akmal; Muliari Muliari
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 6 No 1: May 2024
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v6i1.1859

The research aims to see the effect of using kale (Ipomoea Aquatica), water hyacinth (Eichhornia crassipes) and apu-apu (Pistia statiotes) in the phytoremediation process on water quality in palm oil mill waste. The design used in this research is an experimental design using a non-factorial Completely Randomized Design (CRD). The treatments in the study used 4 treatments and three replications, namely P1: 20 liters of palm oil waste + 600 grams of water spinach, P2: 20 liters of palm oil waste + 600 grams of water hyacinth, P3: 20 liters of palm oil waste + 600 grams of apu-apu, P4: 20 liters of palm oil waste. The observation parameters in this study include pH, DO, Temperature, Nitrate and Phosphate. The results showed that the best aquatic plants in phytoremediation of palm oil wastewater were in treatment B (water hyacinth) and the lowest in treatment D (control). From the results obtained, it can be concluded that water hyacinth is suitable for use as a plant in phytoremediation and is able to improve the quality of palm oil wastewater.

Title

Found 7 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 7 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 7 Documents
Search