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BAMBANG SUNARKO
Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : ANNALES BOGORIENSES

 1 
Identification Of Degradation Pathway Of Vinylacetate Using Bacterial Isolate V2 And Characterization Of The Involved Enzymes Sunarko, Bambang; Sulistinah, Nunik; Nieder, Maria; Meyer, Ortwin
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/13

Abstract

Vinyl acetate is a toxic substance, but has a high commercial value. In this study we show that vinyl acetate is subject to microbial degradation at rates of up to 6.38 and 1 mmol/h per g (dry weight) under aerobic and anaerobic conditions, respectively. It was hydrolyzed by bacterium V2 to ethano, acetaldehyde and acetate. The enzymes involved in the metabolism of vinylacetate were vinyl acetate esterase, aldehyde dehydrogenase, and alcohol dehydrogenase, which localized in the cytoplasmic fraction. The Km values of vinyl acetate esterase and alcohol dehydrogenase were 6.13 mM and 0.24 mM. respectively. Vinyl acetate esterase hydrolyzed the ester to acetate and vinyl alcohol. The Latter isomerized spontaneously to acetaldehyde and was then converted to acetate.The acetaldehyde was disproportionated into ethanol and acetate. The acetate was then converted to acetyl coenzyme A and oxidized through the tricarboxylic acid cycle and the glyoxylate bypass .  
The Development of A Bioassay Based on Heterologous Expression of M2 Ion-Channel Protein Prasetyoputri, Anggia; Yuliaty, Neti; Tuharea, Warda; Febyanti, Alisin Febyanti; Sunarko, Bambang; Atmosukarto, Ines I.C
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.809 KB) | DOI: 10.1234/57

Abstract

Emerging resistant viral  strains combined with the  limited availability of antivirals  in  a pandemic scenario highlight the need for the development of novel influenza antivirals. A bioassay based on the M2 protein of influenza virus  -  a potential target for antivirals - was developed to screen endophytic microbial extracts. M2 can be synthesized using PCR, thus eliminating the need for the handling of infectious specimen. Following cloning of the M2 gene into a pET backbone, the resultant plasmid was transformed into BL21 (DE3) pLyss E. coli  cells.  Cultures of these cells were set up at 37C following inoculation with a starter culture, to reach an OD  at  600nm  (OD) of  0.4-0.6.  Once  at  the  required  OD,  the  culture  was  split  in  two  aliquots  and expression  of  the  M2  protein  was  induced  in  one  of  the  duplicates  with  the  addition  of  isopropyl  β-Dthiogalactopyranoside (IPTG). Bacterial growth was monitored at 60 -minute intervals. Exogenous expression of  the  M2  protein  has  been  reported to  decrease  host  cells  viability,  resulting  in  lower  OD 600 values. Our results  suggest  that  the  M2  protein  was  expressed  and  that  overexpression  of  this  protein  resulted  in consistently lower OD 600  values of induced cultures compared with that of uninduced cultures. Based on this principle,  extracts  can  be  screened  for  their  ability  to  block  M2  function  as  identified  by  increased  OD 600 values.   Keywords: influenza, M2, bioassay, gene synthesis, antiviral
Co-Authors Adityarini, Adityarini AFFANDI, FAKHRUN AFRILIYANI, UCI Agus Suroso Ahmad Thontowi ary yunanto Ashari Ashari Atmosukarto, Ines I. C. Atmosukarto, Ines I.C Cut Misni Mulasiwi Ekaningtyas Widiastuti Febyanti, Alisin Febyanti H. Djoko Windu P. Irawan Indraswati, Denok INDRAWATI GANDJAR Indriati, Suci Ismunarti, Nurbani Aulia Kaban, Joseva Sudiati Kaban, Joseva Sudiati Karsidi Karsidi, Karsidi Matswaya, Akrimi Meyer, Ortwin Munandar, Hendra Nieder, Maria Nofiyanti, Nofa NUNIK SULISTINAH Nur Latifah, Nur Oktafianti, Ika Prasetyoputri, Anggia Putri, Anggi Arinta Putri, Gaviota Gilda RETNO WIDURI Rini Riffiani Sri Martini Subowo, YS Suprijandani Supriyati, Dyah Supriyono, Vincentius Suwaryo, Suwaryo Tambunan, Usman Sumo F Tohir Tohir, Tohir Tuharea, Warda WICAKSONO, BUDIAWAN AJI Wijono, Trimawan Heru Wulandari, Ega Surya Yuliaty, Neti