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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Agrikultura Current Biochemistry Journal Jurnal Agroekoteknologi

Tri Joko Santoso

Molecular Biology Research Group, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Ministry of Agriculture, Bogor 16111, Indonesia

Author-ID : 405203

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education

Published : 7 Documents Claim Missing Document

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Articles

Regeneration Capacity of Indica Rice cv. Bengawan Solo in Two Types of Regeneration Media through Microprojectile Bombardment TRI JOKO SANTOSO; SUDARSONO SUDARSONO; HAJRIAL ASWIDINNOOR; IDA HANARIDA SOMANTRI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Factors affecting the success of gene delivery into cells/tissues of rice mediated by particle bombardment have been investigated. However, their effects on regeneration capacity of rice explants have not been investigated. Objectives of this experiments were to evaluate the effects of particle bombardment on regeneration capacity of indica rice cv. Bengawan Solo calli and the effects of four regeneration media on the development of bombardment calli. Calli of indica rice were initiated from mature seeds on callus-inducing medium. They were transferred on osmoticum containing medium prior to bombardment and maintained on the medium up to two days. Particle bombardment was conducted at 16 cm bombardment distance, 27 cm Hg helium pressure and using standard procedures for preparation of microprojectile particles. The bombarded calli were cultured on RNB or RMS regeneration medium, with or without spermidine addition. Results of the experiment indicated particle bombardment affected the survival of explants of indica rice but did not affect the development of the explants. The development of explants was rather dependent on the regeneration media than on the particle bombardment treatment. RMS medium was better than RNB medium for shoots inducing from bombarded indica rice explants.

Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang Djarot Sasongko Hami Seno; Bambang Padmadi; Dewi Praptiwi; . Sugihartati; . Taufiq; Muhammad Taufan Fatahajudin; Helmy Ramadhan Al Anshary; Tri Joko Santoso; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.

Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia Djarot Sasongko Hami Seno; Satya Nugroho; Tri Joko Santoso; Dimas Adrianto; Dewi Praptiwi; Aniversari Apriana; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern.

Identifikasi gen aroma pada progeni-progeni backcross antara varietas Ciherang dengan Pandan Wangi Djarot Sasongko Hami Seno; Akhmad Endang Zainal Hasan; Tri Joko Santoso; Bram Kusbiantoro; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2) of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang. Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2. RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated) and alleles (homozygot/heterozygot). Further backcross and selfing to obtain BC5F2 is in progress.

Deteksi Transgen (Glu-1Dx5) pada Populasi Padi (Oryza sativa L.) Putative Transgenik Kultivar Fatmawati Nono Carsono; Sri Nurlianti; Inez Nur Indrayani; Ade Ismail; Tri Joko Santoso; Murdaningsih H. Karmana
Agrikultura Vol 21, No 1 (2010): April, 2010
Publisher : Fakultas Pertanian Universitas Padjadjaran

Transformasi gen Glu-1Dx5, pengendali utama karakter elastisitas dan daya mengembang adonan dari gandum, telah berhasil ditransfer ke dalam genom tanaman padi kultivar Fatmawati dengan menggunakan penembakan partikel, dengan tujuan untuk memperbaiki kualitas adonan tepung beras. Galur-galur harapan telah diperoleh, tetapi karena telah mengalami penyerbukan sendiri selama 1-2 generasi yang menyebabkan transgen mengalami segregasi, maka diperlukan upaya pendeteksian transgen pada populasi putative transgenik ini. Upaya ini dapat dilakukan, antara lain dengan menggunakan teknik Polymerase Chain Reaction (PCR) yang memungkinkan perbanyakan fragmen DNA yang spesifik (gen) secara cepat dalam jumlah banyak. Percobaan ini bertujuan untuk mendapatkan tanaman padi transgenik yang memiliki gen Glu-1Dx5 pada dua generasi yang sedang bersegregasi. DNA genom dari 149 tanaman padi (generasi T1 sebanyak 14 tanaman, generasi T2 sebanyak 134 tanaman, dan satu tanaman non-transgenik) telah diekstraksi menggunakan Genomic DNA Purification Kit dari Fermentas. Plasmid pK+Dx5 digunakan sebagai positif kontrol, selain itu digunakan juga enzim Taq DNA polymerase dari Go Green Taq® Master Mix (Promega) dan 2 primer spesifik yang mengamplifikasi coding region dari Glu-1Dx5 (2,5 kb). Hasil percobaan menunjukkan, tanaman padi yang memiliki gen Glu-1Dx5 pada generasi T2-7 sebanyak 26 tanaman, T2-11 : 12 tanaman, T2-12 : 3 tanaman, T2-40 : 3 tanaman dan T2-45 : 5 tanaman. Seluruh tanaman generasi T1 tidak memiliki insert. Hasil ini menunjukkan bahwa gen Glu-1Dx5 sudah terintegrasi ke dalam genom tanaman padi kultivar Fatmawati dan diwariskan dari satu generasi ke generasi berikutnya.

Identification of Aroma Gene (Mutated badh2) and Properties of Aroma on Aromatic BC5F2 Ciherang Jap Mai Cing; Djarot Sasongko Hami Seno; Tri Joko Santoso
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Aromatic rice varieties have some weaknesses such as low productivity, and less resistant to pests and diseases. This study aimed to obtain homozygous strain of BC5F2 Ciherang aromatic through the identification of aroma gene (mutated badh2) and properties of the aroma. Ciherang paddy (nonaromatic paddy) was used as the female parent, whereas Mentik Wangi paddy (aromatic paddy) was used as the male parent. The experiment was conducted in BC5F2 because it is expected to generate plants with properties 98.4% close to female parent. The DNA from five strains of paddy plants BC5F2Ciherang X Mentik Wangi was isolated by a modified CTAB method. The concentration of DNA was determined by measuring absorbance at 260 nm wavelength, while its purity was determined from the ratio of the absorbance at a wavelength of 260/280 nm. PCR-based molecular selection was done by using the Bradbury primers. PCR results showed that of the 250 samples, there were 66 samples had DNA fragment of the same size as that of Mentik Wangi, i.e. 257 bp, 67 samples had the same size as the DNA fragment of Ciherang, i.e. 355 bp, and 117 samples had the same size with the both of DNA fragments, i.e. 257 bp and 355 bp. Plants with amplified 257 bp DNA fragment was subjected to leaf aroma test using 1.7% KOH. The results showed that 42 positive samples, out of 66 samples. Samples positive on leaf aroma test were tested again on rice aroma test. Rice aroma test results showed the majority (85.4%) samples that are positive on leaf aroma test is also positive on the rice aroma test.

RESPON REGENERASI BEBERAPA GENOTIPE DAN STUDI TRANSFORMASI GENETIK TOMAT (Lycopersicon esculentum Mill.) MELALUI VEKTOR Agrobacterium tumefaciens Tri Joko Santoso; A Sisharmini; M Herman
Jurnal Agroekoteknologi Vol 2, No 2 (2010)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTThe development of an effective Agrobacterium transformation for tomato depends on several factors including plant genotype, explant vigor, Agrobacterium strain, selection system and culture conditions. It has been reported that tomato genotype contributed to success of transformation and regenerability in tissue culture. The purpose of this experiment was to study of regeneration response of four tomato genotypes and genetic transformation of tomato through Agrobacterium tumefaciens vector. The experiment of tomato genotype regeneration study was conducted at Biotechnology Laboratory, Asian Vegetables Research and Development Center (AVRDC), Taiwan, meanwhile the experiment of tomato genetic transformation study was conducted at Molecular Biology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Bogor. Three of well-adapted genotypes in Indonesia i.e. Intan, Gondol Hijau and CL6046 were used as regeneration response experimental materials. AVRDC’s genotype, CLN1559A, was used as a control genotype in in-vitro culture. The result of experiment showed that there was variation response on the ability of explant to regenerate and form the shoot among the four genotypes. The control genotype, CLN1559, was the most regenerable genotype that indicated by the highest number of regenerated explants and shoots per explants (90,56% and 0,66), followed respectively by Intan (66,22% and 0,37), CLN6046 (45,94% and 0,26) and Gondol Hijau (17,08% and 0,09). Based on the results, among of the well-adapted genotypes in Indonesia, Intan was the most responsive genotype to be regenerated compared with two other genotypes. Considerably, Intan could be used as an explant for study on genetic engineering tomato through A. tumefaciens vector. The results of genetic transformation study have successfully obtained several explants formed the callus dan shoot on medium selection containing 50 mg/l kanamycin. Nevertheless, transformaton efficiency in the experiment still low which of 55,7 explants in average generated 1,7 shoots (about of 3%) that survive on selection medium (50 mg/l kanamycin). Keywords: Lycopersicon esculentum Mill., Regeneration, Genetic Transformation, Agrobacterium tumefaciens

Co-Authors . Sugihartati . Taufiq A Sisharmini Ade Ismail Akhmad Endang Zainal Hasan Aniversari Apriana Bambang Padmadi Bram Kusbiantoro Dewi Praptiwi Dimas Adrianto Djarot Sasongko Hami Seno HAJRIAL ASWIDINNOOR Helmy Ramadhan Al Anshary IDA HANARIDA SOMANTRI Inez Nur Indrayani Jap Mai Cing M Herman Muhammad Taufan Fatahajudin Murdaningsih H. Karmana Nono Carsono Satya Nugroho Sri Nurlianti Sudarsono Zainal Alim Mas'ud

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