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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Agrikultura Current Biochemistry Journal Jurnal Agroekoteknologi
Tri Joko Santoso
Molecular Biology Research Group, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Ministry of Agriculture, Bogor 16111, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education

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Journal : HAYATI Journal of Biosciences

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Regeneration Capacity of Indica Rice cv. Bengawan Solo in Two Types of Regeneration Media through Microprojectile Bombardment TRI JOKO SANTOSO; SUDARSONO SUDARSONO; HAJRIAL ASWIDINNOOR; IDA HANARIDA SOMANTRI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.957 KB) | DOI: 10.4308/hjb.12.4.157

Abstract

Factors affecting the success of gene delivery into cells/tissues of rice mediated by particle bombardment have been investigated. However, their effects on regeneration capacity of rice explants have not been investigated. Objectives of this experiments were to evaluate the effects of particle bombardment on regeneration capacity of indica rice cv. Bengawan Solo calli and the effects of four regeneration media on the development of bombardment calli. Calli of indica rice were initiated from mature seeds on callus-inducing medium. They were transferred on osmoticum containing medium prior to bombardment and maintained on the medium up to two days. Particle bombardment was conducted at 16 cm bombardment distance, 27 cm Hg helium pressure and using standard procedures for preparation of microprojectile particles. The bombarded calli were cultured on RNB or RMS regeneration medium, with or without spermidine addition. Results of the experiment indicated particle bombardment affected the survival of explants of indica rice but did not affect the development of the explants. The development of explants was rather dependent on the regeneration media than on the particle bombardment treatment. RMS medium was better than RNB medium for shoots inducing from bombarded indica rice explants.
Co-Authors . Sugihartati . Taufiq A Sisharmini Ade Ismail Akhmad Endang Zainal Hasan Aniversari Apriana Bambang Padmadi Bram Kusbiantoro Dewi Praptiwi Dimas Adrianto Djarot Sasongko Hami Seno HAJRIAL ASWIDINNOOR Helmy Ramadhan Al Anshary IDA HANARIDA SOMANTRI Inez Nur Indrayani Jap Mai Cing M Herman Muhammad Taufan Fatahajudin Murdaningsih H. Karmana Nono Carsono Satya Nugroho Sri Nurlianti Sudarsono Zainal Alim Mas'ud