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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Journal of Microbial Systematics and Biotechnology ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Journal of Marine Research

Agus Trianto

Department Of Marine Science, Faculty Of Fisheries And Marine Science, Diponegoro University, St. Prof. Soedarto, SH., Tembalang, Semarang 50269, Central Java, Indonesia

Author-ID : 405510

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology

Published : 40 Documents Claim Missing Document

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Articles

Title

Skrining Bahan Anti Kanker pada Berbagai Jenis Sponge dan Gorgonian Terhadap L1210 Cell Line Agus Trianto; Ambariyanto Ambariyanto; Retno Murwani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 3 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Sejarah evolusi yang panjang pada biota laut menyebabkan biota laut mempunyai keanekaragaman molekul yang sangat tinggi. Potensi biota laut tersebut sebagai sumber obat anti kanker menjadi objek penelitianpenting dalam tahun-tahun terakhir. Bahan yang dipergunakan dalam penelitian ini adalah sponge dan gorgonian yang dikoleksi dari perairan Jepara pada kedalaman 1-3 m dan di perairan Labuhan Bajo, Florespada kedalaman 3-38 m. Sampling dilakukan dengan Skin diving dan SCUBA diving. Sampel kemudian diekstrak dengan metanol. Selanjutnya ekstrak diujikan terhadap sel kanker leukemia (L-1210 cell line) dengankonsentrasi 0, 1, 5 dan 10 ppm. Uji dilakukan pada media RPMI lengkap dan penghitungan daya hambat dilakukan dengan metoda direct counting. Ekstrak yang diperoleh dari sponge dan gorgonian berkisar antara 0,55 – 24,7% dari berat kering atau 0,36-7,34% dari berat basahnya. Seluruh ekstrak dari sampel-sampel mampu menghambat pertumbuhan L1210 cell line dan layak untuk pemurnian lanjut. Ekstrak metanol tiga jenis sponge (Xestospongia sp2 dan Phyllospongia sp1 dan UP8) dan fraksi etil asetat dari ekstrak gorgonian I. hippuris mempunyai IC-50 < 3 mg/mL, ekstrak metanol dari delapan jenis sponge (Agelas nakamurai, Ircina ramosa, A06, Phyllospongia lamellosa , Phyllospongia sp, UP9, Calispongia sp dan Fascaplynopsis sp) mempunyai IC-50 < 5 mg/mL, dan ekstrak metanol dari lima jenis sponge (Hyrtios erecta, Xestospongia sp, Cladocroce sp, Oceanapia cf. Amboiensis dan Haliclona sp.) dan fraksi air dari ekstrak gorgonian I. Hippuris mempunyai IC-50 < 10 mg mL Satu sponge Xestospongia sp 1 mengandung ekstrak yang mempunyai IC - 50 > 10 mg/mL.Kata kunci : Sponge, Gorgonian, L-1210, sel kanker, IC-50The long history of the evolution of marine organisms has made these organisms as a source of high molecular diversity. In search of a new anti cancer drugs, these organisms has become an intense object of research.Sponges and the gorgonian were colllected from Jepara and Labuan Bajo-Flores water on a depth of 1-3 m and 3-38 m repectively by Skin and SCUBA diving. The samples were extracted with methanol. Theextract were assayed against cancer cell (L-1210 cell line) with tested concentration range of 0, 1, 5 and 10 ppm. The cell line was cultured in complete RPMI media and growth inhibition was measured by directcounting. Methanol extract of sponges and gorgonian yielded approximately 0.55 – 24.7 % dry weight or 0,36 – 7.34 % wet basis. All methanol extract of the samples could inhibit the growth of L1210 cell line and is potential for further purification. Methanol extract from 3 species of sponges (Xestospongia sp2 dan Phyllospongia sp1 and UP8) and one ethyl acetate fraction of I. hippuris had IC-50 value < 3 mg/mL. Methanol extract of 8 species of sponges (Agelas nakamurai, Ircina ramosa, A06, Phyllospongia lamellosa, Phyllospongia sp, UP9, Calispongia sp dan Fascaplynopsis sp) had IC-50 < 5 mg/mL, methanol extract of 5 species of sponges (Hyrtios erecta, Xestospongia sp, Cladocroce sp, Oceanapia cf. Amboiensis and Haliclona sp.) and one water extract of I. hippuris had IC-50 < 10 mg mL. Methanol extract of one species of sponge (Xestospongia sp 1) had IC-50 > 10 mg/mL.Key words : Sponge, Gorgonian, L-1210, Cancer cell, IC-50

Screening of Antibacterial MDR derived from Sponge Associated Fungus of Riung Water, Nusa Tenggara Timur Khoeruddin Wittriansyah; Agus Trianto; Sekar Widyaningsih; Ocky Karna Radjasa; Rudhi Pribadi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Marine sponge-associated fungi are the sources of bioactive compounds with various pharmacologicals potency. This study aimed to isolate the sponge-associated fungi as the producer of the MDR anti-bacterial compounds. The associated fungi were isolated from the sponges collected from Riung water, Nusa Tenggara Timur. Five of the best isolates were cultured on MEA to obtain the methanolic extract for further studies. The antagonistic test was conducted using overlay method towards the MDR Staphylococcus aureus and Escherichia coli. A total of 33 fungi were isolated from 19 sponge specimens. The antagonistic test showed that 19 isolates were active against both S. aureus and E. coli, and 13 of them were merely active against one of the bacteria. However, only five isolates have strong activity against one or both of the bacteria. The KN-15-3 had the strongest activity against S. aureus (18.75±0.777mm) and E. coli (15.10±0.141mm) at the concentration of 400 μg.disc-1 so it can be developed further as a source of drug candicate. Keywords: Fungi symbiont, Sponges, MDR Antibacterial, Staphylococcus aureus, Escherichia coli.

The Potential of Cytotoxin and Antiviral in Sargassum polycystum and Sargassum ilicifolium’s Polysaccharides Extract Dwi Lestari Widya Ningsih; Agus Trianto; Ita Widowati; Rexie Magdugo; Anicia Hurtado; Christel Marty; Nathalie Bourgougnon
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 25, No 3 (2020): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.25.3.91-96

Marine algae known as one producers of bioactive compounds. This study aims to analyze the cytotoxicity and antiviral activity in Sargassum polycystum and Sargassum ilicifolium tested with Herpes Simplex Virus (HSV). The polysaccharides extract of algae was used in this study, as sulfated polysaccharides have been reported has bioactivity. Cytotoxicity either antiviral could be correlated with the sulfate content as well as nature and chemical composition of the polysaccharides. Cytotoxicity and antiviral analysis based upon cell viability. Using the Vero cell / HSV-1 model, cytotoxicity was evaluated by incubating cellular suspensions (3.5×105 cells.mL-1) with various dilutions (concentration from 1 to 500 µg.mL-1, four wells per concentration) of fractions in 96-well plates (72h, 37°C, 5% CO2) in Eagle's MEM containing 8% FCS. The cells were examined daily under a phase-contrast microscope to determine the minimum concentration of hydrolysate dry matter that induced alterations in cell morphology, including swelling, shrinkage, granularity and detachment. Algae S. illicifolium was found to have the highest cytotoxic content in each solution compared to S. polycystum. Algae S. illicifolium in KOH 4M (cellulose) reached 2,707 µg.ml-1, then HCl pH 2 (fucoidan) was 2,477 µg.ml-1, then CaCl2 2% (fucoidan) was 2,362 µg.ml-1, and in Na2CO3 3% (alginates) was 2,134 µg.ml-1. For antiviral, S. polycystum contained the highest antiviral compounds compared to S. illicifolium with KOH 4M (cellulose) solution was reached 67.02 µg.ml-1. Then in Na2CO3 3% (alginates) which was 33.25 µg.ml-1, then CaCl2 2% (fucoidan) which was 31.62 µg.ml-1,and HCl pH 2 (fucoidan) was 30.08 µg.ml-1. After all, the highest bioactivity compounds was found with KOH 4M (cellulose) for cytotoxicity in S. ilicifolium and antiviral activity in S. polycystum.

Laju Pertumbuhan dan Kelulushidupan Transplan Spons Amphimedon sp. (Growth and Survival of Sponge Amphimedon sp. Transplants) Agus Trianto; Radisya N Nissa; Diah Permata Wijayanti; Azis Rifai; Dwi Haryo Ismunarti; Destio .
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 4 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Spons adalah salah satu sumber bahan hayati laut yang potensial. spons Amphimedon sp. terbukti memiliki potensi sebagai senyawa bioaktif anti kanker. Namun, pemanfaatan spons dari alam akan memberikan dampak yang buruk terhadap lingkungan, khususnya populasi organisme tersebut. Melalui budidaya spons dapat diaplikasikan untuk menyediakan bahan bioaktif dalam jumlah yang cukup secara berkesinambungan. Penelitian ini bertujuan untuk mengetahui laju pertumbuhan dan tingkat kelulushidupan spons Amphimedon sp. yang dibudidayakan melalui transplantasi secara in situ di perairan Pulau Panjang, Jepara, Jawa Tengah. Spons ditransplantasikan pada perairan laut dengan jarak 6 m dan 1 m dari dasar, dengan 2 ukuran awal eksplan (3cm x 3cm x 1,5cm dan 6cm x 6cm x 1,5cm). Laju pertumbuhan dihitung berdasarkan pertambahan volume eksplan. Hasil penelitian menunjukkan bahwa ukuran awal eksplan memberikan pengaruh yang nyata terhadap laju pertumbuhan. Sebaliknya, perbedaan kedalaman tidak memberikan pengaruh yang nyata terhadap laju pertumbuhan spons. Laju pertumbuhan eksplan spons Amphimedon sp berkisar 3,01±1,60 cm3.hari-1 sampai dengan 3,43±1,08 cm3.hari-1. Kelulushidupan eksplan spons mencapai 100%. Hasil ini menegaskan bahwa untuk usaha budidaya spons sebaiknya menggunakan ukuran awal eksplan besar. Disamping itu perairan Pulau Panjang terbukti memiliki perairan yang sesuai untuk budidaya spons. Kata kunci: akuakultur, eksplan, spons, bahan bioaktif Sponge is known as important marine natural product sources. Sponge Amphimedon sp. has been proven to have anticancer substances. However, direct exploitation of sponge from nature will give a bad impact to the marine environment. Sponge aquaculture can be applied for sufficiently and sustainably supply of bioactive compounds. In order to obtain data on growth and survival rates of transplanted sponge Amphimedon sp. in Panjang Island-Jepara waters, this in situ research was conducted. The sponges were explanted at 6 m and 1 m above the sea floor with two initial approximate size (3cm x 3cm x 1.5cm and 6cm x 6cm x 1.5cm). The growth rate was indicated by volumetric increment. The results showed that initial explants size gave a significant effect on the growth rates of the sponge. On the other hand, two different depths of culture did not give significant effect. The explant growth rates range from 3.01±1.60 cm3.day-1 to 3.43±1.08 cm3.day-1. Survival rate of the sponge during the experiment was 100%. This result confirms that for the cultivation of sponges should use larger explants initial size. It also suggests that Panjang Island water has proven suitable for sponges cultivation activities. Keywords: aquaculture, explant, sponges, bioactive substance

Co-Authors Agus Sabdono Akhmad Tri Prasetyo Aldi Nuary Nuary Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Ambariyanto , Ambariyanto Ambariyanto Andi Afriandi Andi Afriandi Anicia Hurtado Aninditia Sabdaningsih Ayu Charismawaty Azis Rifai Aziz Rifai Bahry, Muhammad Syaifudien Bimo Saskiaoktavian Christel Marty Destio . Desy Wulan Triningsih Diah Permata Wijayanti Diah Permata Wijayanti Dwi Haryanti Dwi Haryo Ismunarti Dwi Lestari Widya Ningsih Dwi Susilaningsih Elfanando Rizky Juliantara Sucipto Elis Indrayanti Endang Supriyantini Farrastasya Muflihul Azzami Fran Ciputra Tambunan Frans Alexander Nainggolan Gita Wismayanti Humairah Arifia Sabiladiyni Irene Ulsadriatny Irwani Irwani Isai Yusidharta Ita Puspitasari Ita Widowati Jusup Supriyanto Jusup Supriyanto Lilik Maslukah Ma'ruf, Widodo Farid Mada Triandala Sibero Mada Triandala Sibero Mahadika Fanindhita Sany McCauley, Erin Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Syaifudien Bahry Munasik Munasik Nathalie Bourgougnon Ni Komang Tri Utami Nining Nursalim Nugraha Ridho Ikhsani, Nugraha Ridho Nugroho Agus D Nugroho Hendartono Nugroho Hendartono Nur Taufiq-Spj Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Popi IL Ayer Puspitasari, Ita Rachmat Afriyanto Raden Ario Radisya N Nissa Raja Aditia Sahala Siagian Retno Murwani Rexie Magdugo Rizky Rifatma Jezzi Rohimat Rohimat Roselina Nadya Kristi Rudhi Pribadi Rudhi Pribadi Sabiladiyni, Humairah Arifia Sakti Imam Muchlisin Sekar Widyaningsih Sembiring, Yudhawira Bhaskara Septhy Kusuma Radjasa Shofiatun Nimah Sri Sedjati Subagiyo Subagiyo Sucipto, Elfanando Rizky Juliantara Tambunan, Fran Ciputra Taufiq-Spj, Nur Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Yudhawira Bhaskara Sembiring

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Journal : ILMU KELAUTAN: Indonesian Journal of Marine Sciences