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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Journal of Microbial Systematics and Biotechnology ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Journal of Marine Research

Agus Trianto

Department Of Marine Science, Faculty Of Fisheries And Marine Science, Diponegoro University, St. Prof. Soedarto, SH., Tembalang, Semarang 50269, Central Java, Indonesia

Author-ID : 405510

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Pemanfaatan Jamur Simbion Sponge dalam Bioisomerasi Asam Lemak pada Dedak untuk Menghasilkan Asam Lemak Cis Agus Trianto; Sri Sedjati; Ocky Karna Radjasa; Rachmat Afriyanto; Sakti Imam Muchlisin; Septhy Kusuma Radjasa; Muhammad Syaifudien Bahry
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Utilization of Sponges Symbiotic Fungus in the Bioisomeration of Fatty Acids in Brans to Produce Cis Fatty Acid Trans fatty acids are known to have a negative impact on human health such as triggering the coronary heart and blood vessel related diseases. However, many food and animal feed contain trans fatty acids. Bran, which is widely used as animal feed has high fatty acid content and the majority are trans fatty acids. This study aims to determine the potential of Trichoderma harzanum, a sponge symbiont fungus, in the process of fatty acid bioconversion in bran. Bran was fermented in saline and non-salin condition for 15 days in the room temperature. Both fermented and not bran was extracted with methanol, and the extracts were concentrated with rotary evaporator. Fatty acid extracts were analyzed on the GC with hydrolysis and methylation as pre-treatments. The test results showed the fatty acid composition changed, and the was formation of new fatty acids. The bioisomeration process occurs in the Trans-9-Elaidic acid Methyl esther into Cis-9-Oleic Methyl ester. Cis-9-oleic acid is known as oleic acid which is widely found in olive and sunflowers oil. The fungus has potential as biocatalys for production of cis fatty acid. Asam lemak trans dikenal mempunyai dampak negatif terhadap kesehatan manusia seperti memicu terjadinya penyakit jantung koroner dan penyakit terkait pembuluh darah. Namun asam lemak trans banyak terkandung dalam berbagai jenis bahan makanan maupun pakan ternak diantaranya adalah dedak. Dedak banyak digunakan sebagai pakan ternak mempunyai kandungan asam lemak yang tinggi dan mayoritas adalah asam lemak trans. Penelitian ini bertujuan untuk mengetahui potensi jamur simbion spons Trichoderma harzanum dalam proses biokonversi asam lemak pada dedak. Fermentasi dedak dilakukan dalam kondisi salin dan unsalin selama 15 hari pada suhu ruang. Dedak yang difermentasi maupun tidak kemudian diekstrak dengan metanol kemudian dipekatkan dengan rotari eveporator. Ekstrak asam lemak degan GC dengan pre-treatment meliputi hidrolisis dan metilasi. Hasil uji menunjukan adanya perubahan komposisi dan terbentuknya asam lemak baru. Proses bioisomerasi terjadi pada Trans-9-Elaidic acid Methyl estermenjadi Cis-9-Oleic Methyl ester. Cis-9-oleic acid yang dikenal dengan asam oleat yang banyak terdapat dalam minyak zaitun dan bunga matahari. Jamur T. Harzianum mempunyai potensi sebagai biokatalis untuk memproduksi asam lemak cis.

Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli Mada Triandala Sibero; Aninditia Sabdaningsih; Ocky Karna Radjasa; Agus Sabdono; Agus Trianto; Subagiyo Subagiyo
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted using ethyl acetate. The antibacterial activity of crude extracts was tested using the paper disc diffusion method while bioactive compounds were characterized using the phytochemical method. The results showed that the antibacterial activity of the broth media extract performed a greater activity than the crude extract from the mycelium. The crude extract from mycelia only contained flavonoid and phenol hydroquinone compounds while the crude extract from broth media contains alkaloids, flavonoids, phenols hydroquinone and saponins. Kapang Trichoderma asperellum MT02 telah dilaporkan memiliki potensi sebagai penghasil senyawa antibakteri melawan Extended Spectrum β-Lactamase (ESBL) E. coli berdasarkan hasil penapisan melalui metode agar plug. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kasar kapang T. asperellum MT02 serta komposisi golongan senyawa bioaktif yang dimiliki. Kapang dikultur pada media Malt Extract Broth (MEB) (statis, 27 oC, 15 hari) di mana metabolit intraseluler dari miselium diekstrak menggunakan metanol sedangkan metabolit ekstraseluler dari media kaldu diekstrak menggunakan etil asetat. Aktivitas antibakteri ekstrak kasar diuji menggunakan metode difusi kertas cakram sedangkan senyawa bioaktif dikarakterisasi menggunakan metode fitokimia. Hasil penelitian menunjukkan bahwa aktivitas antibakteri asal ekstrak media kaldu lebih baik dibandingkan ekstrak kasar asal miselium kapang. Ekstrak kasar kapang asal miselia hanya mengandung senyawa golongan flavonoid dan fenol hidrokuinon sedangkan ekstrak kasar asal media kaldu mengandung alkaloid, flavonoid, fenol hidroquinon dan saponin.

Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria Sri Sedjati; Ambariyanto Ambariyanto; Agus Trianto; Endang Supriyantini; Ali Ridlo; Muhammad S. Bahry; Rizky Rifatma Jezzi; Mahadika Fanindhita Sany
Jurnal Kelautan Tropis Vol 23, No 1 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i1.7049

Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3, M4) produce secondary metabolites with varying quantities and antimicrobial activity. The inhibition zone of the produced extract with 500 μg/disc concentration ranged from 7.14 to 10.32 mm. The strongest potential was shown by ethyl acetate extract from isolates cultured with M2 (9 days cultivation), of which is able to produce 10.32 mm inhibition zones against S. aureus and methanol extracts from isolates cultured with M4 (6 days cultivation), and able to produce 10.05 mm inhibition zones against K. pneumoniae. In conclusion, the fungus Aspergillus flavus can be culture using fish broth added glucose and cassava infusion water added peptone media to potentially produce antibacterial compounds against MDR pathogens.

Aktivitas Antagonis Bakteri yang Berasosiasi dengan Teritip (Balanus sp.) terhadap Bakteri Escherichia coli dan Bacillus cereus Gita Wismayanti; Sri Sedjati; Agus Trianto
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Marine invertebrates are the most productive source of bioactive compounds. However, most of the compounds originally produced by the microorganisms living associated with the invertebrates. Barnacle is an invertebrate that has a unique interaction with bacteria. Escherichia coli and Bacillus cereus are the cause of the foodborne disease that causes of remarkedly losses in the food industry. The barnacle was collected from Panjang Island, Jepara. The bacterial isolates were obtained by serial dilution followed by isolation based on morphological characteristics. The antagonistic assay to E. coli dan B. cereus was utilized for screened the isolates. Then, the active isolates were cultured for the further test with the disc diffusion agar method of the supernatant followed by the extracts. The most active isolates were identified based on molecular method. A total of 14 isolates were obtained from the Balanus sp., which six of them have activity against the E. coli and B. cereus. The isolates TJ 5.4 and TJ 5.5 have the strongest activity the bacteria. Base on the analyses of the BLAST and phylogenetic tree the isolates showed 99% homology the Bacillus wiedmannii (TJ 5.4) and Lysinibacillus macroides (TJ 5.5). Invertebrata laut merupakan salah satu sumber bahan bioaktif yang paling produktif. Sebagian senyawa yang terdapat dalam biota laut, sejatinya diproduksi oleh mikroorganisme yang hidup berasosiasi dengan biota laut tersebut. Teritip merupakan salah satu hewan invertebrata yang memiliki berbagai interaksi unik dengan bakteri. Bakteri Escherichia coli dan Bacillus cereus merupakan dua dari beberapa bakteri patogen yang sering menjadi agen penyebab foodborne disease. Teritip dikoleksi dari Pulau Panjang, Jepara. Isolat bakteri diperoleh dengan metoda pengenceran bertingkat dan dilanjutkan isolasi berdasarkan karakteristik morfologis. Uji antagonis terhadap E. coli dan B. cereus digunakan untuk menskrining bioaktivitas isolat. Isolat yang aktif dikultur untuk uji lanjut yaitu supernatant dan dilanjutkan ekstraknya dengan metoda disc diffusion agar. Bakteri yang paling aktif diidentifikasi secara molekuler untuk mengetahui spesiesnya. Sebanyak 14 isolat bakteri diperoleh dari Balanus sp. dimana enam diantaranya memiliki aktivitas terhadap bakteri uji. Isolat TJ 5.4 dan TJ 5.5 memiliki aktivitas paling kuat terhadap E. coli dan B. cereus. Berdasarkan pengolahan sekuen dan analisis pohon filogenetik, dua isolat bakteri tersebut memiliki homologi 99% terhadap bakteri Bacillus wiedmannii (TJ 5.4) dan Lysinibacillus macroides (TJ 5.5).

Prevalensi Penyakit Karang di Pulau Menjangan Besar Karimunjawa Nining Nursalim; Agus Trianto; Muhammad Syaifudien Bahry; Dwi Haryanti; Raden Ario; Raja Aditia Sahala Siagian; Akhmad Tri Prasetyo
Jurnal Kelautan Tropis Vol 25, No 1 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i1.13208

Coral disease can cause coral damage on Menjangan Besar Island, Karimunjawa. Floating net cages adjacent to coral reefs can affect coral health. Floating net cages can affect water quality by adding nutrients, such as nitrate (205.6 and phosphate 39.2/ton fish production). Nutrients and environmental factors can trigger and cause coral disease. The purpose of this study was to determine the effect of the existence of floating net cages on the prevalence of coral disease on Menjangan Besar Island, Karimunjawa. The research was conducted using a survey method, data collection using a proportional method, the results of the data will be analyzed descriptively. The results of the study found Black Band Disease, Brown Band Disease, Ulcerative White Pox, White syndrome, Yellow Bloch Disease, Pigmentation responses, White Plague and White Band Disease. Water parameters such as temperature ranged from 30.71-30.750C, salinity 32.7-33.20/00, current velocity 0.03-0.06 m/s, nitrate 0.586-1,128 mg/L and phosphate 0.064-0.133 mg/ L. The most common disease is Ulcerative White Pox. The prevalence of coral disease is influenced by the presence of floating net cages, it can be seen in A1, A2 and A3 with prevalence values (39.85, 43.61 and 33.14) which are higher than B1 and B2 (14.41 and 10,6). Penyakit karang dapat menyebabkan kerusakan karang di Pulau Menjangan Besar Karimunjawa. Karamba jaring apung yang berdampingan dengan terumbu karang dapat mempengaruhi kesehatan karang. Karamba jaring apung dapat mempengaruhi kualitas air dengan menambah nutrien, seperti Nitrat (205,6 dan fosfat 39,2/ton produksi ikan. Nutrien dan faktor lingkungan dapat menjadi pemicu dan penyebab penyakit karang. Tujuan penelitian ini untuk mengetahui pengaruh keberadaan karamba jaring apung terhadap prevelensi penyakit karang di Pulau Menjangan Besar Karimunjawa. Penelitian dilakukan dengan metode survei, pengambilan data dengan metode porposif, hasil data akan disanalisis secara deskiptif. Hasil penelitian ditemukan penyakit Black Band Disease, Brown Band Disease, Ulcerative White Pox, White syndrome, Yellow Bloch Disease, Pigmentation responses, White Plague dan White Band Disease. Parameter perairan seperti temperatur berkisar 30,71-30,750C, salinitas 32,7-33,20/00, kecepatan arus 0,03-0,06 m/s, nitrat 0,586-1,128 mg/L dan fosfat 0,064-0,133 mg/L. Penyakit paling banyak ditemukan adalah Ulcerative White Pox. Prevelensi penyakit karang dipengaruhi oleh keberadaan karamba jaring apung, dapat terlihat pada A1, A2 dan A3 dengan nilai prevalensi (39,85, 43,61 dan 33,14) yang lebih tinggi jika dibandingkan dengan B1 dan B2 (14,41 dan 10,6).

Skrining Aktivitas Antibakteri Dan Identifikasi Sponge Dari Teluk Kupang Agus Trianto; Ni Komang Tri Utami; Ocky Karna Radjasa; Isai Yusidharta; Wiratno Wiratno
Jurnal Kelautan Tropis Vol 19, No 2 (2016): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Recently, many research proved that bacteria Escherichia coli and Staphylococus aureus have resistant to antibiotics, called Multi Drug Resistance (MDR). The bacteria cause various diseases both in humans and animals. Exploration and development of new antibiotics is needed, one which is derived from a sea sponge. This research aims to determine the potential of antibacterial sponge extracts from the waters of Kupang, East Nusa Tenggara against E. coli and S. aureus MDR, as well as being able to identify the sponge. Maceration method was employed on extraction of the sponges, then the extract were tested against E. coli and S. aureus. The extracts content of sponge varied from 0.4% to 5,19%. Anti bacterial test showed that the sponge K14-52 has the highest inhibition zone of 10,43 ± 0,26 mm and 9,38 ± 0,57 mm and against the E. coli and S. Aureus, respectively, at a concentration of 500 ug/disk. Based on macroscopic and microscopic anayses, the K14-52 sponge is identified as Rhabdastrella globostellata. a Demospongia sponge. The sponge has special characteristic i.e. globular shape with large oscula on the top of the sponge and ostia along the surface. The sponge also characterized with makrosclera monoaxon spikule (hastate oxea, centrotylote oxea, oxea fusiform gyrus) and mikrosclera tertaxon oxyaster spikule. Saat ini banyak ditemukan bakteri Escherichia coli dan Staphylococus aureus yang resisten terhadap antibiotik atau disebut Multi Drugs Resistance (MDR). Bakteri tersebut dapat menimbulkan berbagai penyakit baik pada manusia maupun hewan. Eksplorasi dan pengembangan sumber antibiotik baru sangat diperlukan, salah satunya yang berasal dari sponge laut. Penelitian ini bertujuan untuk mengetahui potensi antibakteri ekstrak sponge dari Perairan Kupang, Nusa Tenggara Timur terhadap bakteri E. coli dan S. aureus MDR, serta mampu mengetahui jenis sponge. Ekstraksi dilakukan dengan metoda maserasi kemudian ekstrak diuji terhadap bakteri patogen. Uji antibakteri menunjukkan bahwa sponge K14-52 memiliki potensi antibakteri paling tinggi dengan zona hambat sebesar 10,43±0,26 mm terhadap bakteri E. coli dan 9,38±0,57 mm terhadap bakteri S. aureus pada konsentrasi 500 µg/disk. Sponge K14-52 diidentifikasi sebagai Rhabdastrella globostellata berdasarkan analisis secara makroskopis dan mikroskopis. Ciri khas sponge tersebut adalah mempunyai bentuk bulat dengan ostia yang tersebar pada permukaan dan satu Oskula besar pada bagian atas sponge. Secara mikroskopis sponge tersebut memiliki makrosklera monoaxon spikula (hastate oxea, centrotylote oxea, fusiform oxea) dan mikrosklera tertaxon spikula Oxyaster.

Co-Authors Agus Sabdono Akhmad Tri Prasetyo Aldi Nuary Nuary Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Ambariyanto , Ambariyanto Ambariyanto Andi Afriandi Andi Afriandi Anicia Hurtado Aninditia Sabdaningsih Ayu Charismawaty Azis Rifai Aziz Rifai Bahry, Muhammad Syaifudien Bimo Saskiaoktavian Christel Marty Destio . Desy Wulan Triningsih Diah Permata Wijayanti Diah Permata Wijayanti Dwi Haryanti Dwi Haryo Ismunarti Dwi Lestari Widya Ningsih Dwi Susilaningsih Elfanando Rizky Juliantara Sucipto Elis Indrayanti Endang Supriyantini Farrastasya Muflihul Azzami Fran Ciputra Tambunan Frans Alexander Nainggolan Gita Wismayanti Humairah Arifia Sabiladiyni Irene Ulsadriatny Irwani Irwani Isai Yusidharta Ita Puspitasari Ita Widowati Jusup Supriyanto Jusup Supriyanto Lilik Maslukah Ma'ruf, Widodo Farid Mada Triandala Sibero Mada Triandala Sibero Mahadika Fanindhita Sany McCauley, Erin Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Syaifudien Bahry Munasik Munasik Nathalie Bourgougnon Ni Komang Tri Utami Nining Nursalim Nugraha Ridho Ikhsani, Nugraha Ridho Nugroho Agus D Nugroho Hendartono Nugroho Hendartono Nur Taufiq-Spj Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Popi IL Ayer Puspitasari, Ita Rachmat Afriyanto Raden Ario Radisya N Nissa Raja Aditia Sahala Siagian Retno Murwani Rexie Magdugo Rizky Rifatma Jezzi Rohimat Rohimat Roselina Nadya Kristi Rudhi Pribadi Rudhi Pribadi Sabiladiyni, Humairah Arifia Sakti Imam Muchlisin Sekar Widyaningsih Sembiring, Yudhawira Bhaskara Septhy Kusuma Radjasa Shofiatun Nimah Sri Sedjati Subagiyo Subagiyo Sucipto, Elfanando Rizky Juliantara Tambunan, Fran Ciputra Taufiq-Spj, Nur Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Yudhawira Bhaskara Sembiring

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