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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Hortikultura Indonesia Journal of Tropical Crop Science Menara Perkebunan Jurnal Pusat Inovasi Masyarakat (JPIM)

Ni Made Armini Wiendi

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor

Author-ID : 416879

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences

Published : 21 Documents Claim Missing Document

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Articles

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Study of Oil Palm (Elaeis guineensis Jacq) In Vitro Embryogenesis using Young Leaves Explants Wiendi, Ni Made Armini; Constantin, Mondjeli; Wachyar, Ade
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

This study reported in vitro embryogenesis of oil palm using young leaves as explants. Explants were grown in solid modified MS or Eeuwens medium containing different concentrations of NAA and 2,4-D, i.e. media C1, C2, C3, C4 and C5, M1, M2, M3 and M4, to induce embryogenic calli. Compact and pearly-white, globular calli were obtained from the youngest leaf explants 28 weeks after culture.C1 media (MS medium + 107.41 µM of NAA + 100 mg.L-1 of asparagine + 100 mg.L of glutamine-1) produced the highest percentage of calli formation (30.56%), whereas C4 media (C1 supplemented with 67.86 µM of 2,-D ) was the optimal media for embryogenic callus induction. Direct embryoids were obtained from slightly older leaf explants on the C3 media containing NAA after 36 weeks of culture. However, four subcultures using the same medium with gradual reduction of auxin concentration were not successful to develop embryogenic callus and embryoid cells during the course of this study.

Proliferation of Protocorm-Like Bodies of Dendrobium mannii and Dendrobium mirbelianum in Chitosan-containing Media In Vitro Rahmah, Syifaur; Wiendi, Ni Made Armini; Suwarno, Willy B.; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 2 No 3 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Dendrobium mannii and Dendrobium mirbelianum were rare and endangered orchid species. In vitro micro propagation could be used to conserve and proliferate these species. The aims of this study were to determine the effects of chitosan on proliferation of the protocorm-like-bodies (PLBs) of in vitro grown Dendrobium mannii and D. mirbelianum. Chitosan has been reported as an important component to promote orchid growth in vitro. In this study chitosan was included in the in-vitro media at the concentrations of 0, 1, 2, 3, and 4 mg.L-1. The highest PLBs formation, clump diameter, and fresh weight on D. mannii were obtained from explants cultured on medium supplemented with 1 mg.L-1 chitosan. After 14 weeks of culture, fresh weight, clump diameter and average number of PLBs of D.mannii were 29.52 %, 33.00 % and 35.88% greater at chitosan (1 mg.L-1) compared to control media. D. mirbelianum treated with low chitosan concentrations (1 and 2 mg.L-1) had 65.71% more leaves than those without chitosan treatment. Chitosan, however, had an adverse effects on growth of both species at 4 mg.L-1. Medium contain 4 mg.L-1 chitosan induced PLBs proliferation of D.mannii, but inhibited PLBs proliferation of D.mirbelianum. These results suggested that chitosan had important roles in PLB proliferation and could be used for the propagation of D. mannii PLBs in vitro. Keywords: rare orchid species, in-vitro

Evaluation of Growth, Flowering and Seed Morphology of Batflower, Tacca chantrieri Andre Ajisyahputra, Nikko Rizky; Palupi, Endah Retno; Krisantini, Krisantini; Wiendi, Ni Made Armini; Mulyono, Joko
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Tacca chantrieri is a perennial plant belongs to Taccaceae family that has the potential to be marketed as an ornamental plant due to its unique shape and dark color inflorescence. To date, very limited publication on T. chantrieri are available. The aim of this research was to examine plant growth pattern and flower phenology of T. chantrieri from West Borneo, Indonesia, and from Queensland, Australia in relation to their potential development as ornamental pot plants. The study was extended to examine T. chantrieri seed morphology with an inclusion of an accession from Thailand. The field experiment was conducted at the Leuwikopo experimental station in Darmaga, Bogor, Indonesia. Seed morphology examination was conducted at the Histology Laboratory of Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. T. chantrieri from West Borneo blooms six times a year whereas the Queensland accession twice a year. West Borneo accession had smaller bractea, fewer numbers of flowers per inflorescence and shorter flower stalk than Queensland accession. In addition, T. chantrieri Queensland grew faster than Borneo with the one leaf formed every two to three weeks, whereas it was at least four weeks in the Borneo accession. Both accessions were susceptible to leaf brown spot disease caused by Cercospora taccae. T. chantrieri Queensland seeds are oval dark brown, whereas West Borneo and Thailand accessions were shaped like kidneys (reniform) with a lighter brown color. This study demonstrated that there is great diversity in T. chantrieri including plant morphology, seed shape and color, which are important for species identification and for development of T. chantrieri as ornamental pot plant.

In Vitro Adventitious Shoot Proliferation of Three Basil Species (Ocimum sp. L.) by Addition of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) Wiendi, Ni Made Armini; Putri, Devina Daratyama
Journal of Tropical Crop Science Vol 4 No 3 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Basils are mainly used as food flavoring and source of aromatic oil, but the leaves have also been used for herbals and cosmetics. Basil is propagated by seeds but the germination is often low in the field. This study aims to investigate the effect of different concentrations of NAA (Naphthalene Acetic Acid) and BAP (Benzyl Amino Purine) to induce the adventitious shoot proliferation of three species of basil (Ocimumsp. L.) in vitro. The research was conducted at the Tissue Culture Laboratory II of the Department of Agronomy and Horticulture, Bogor Agricultural University (IPB) from November 2016 till October 2017. This research consisted of three separate experiments with one basil species for each experiment, Lemon Basil, “Greek Minette”, and “Thai Siam Queen”. The experiments were organized in a completely randomized block design with two factors: NAA concentrations (0, 0.5, and 1 mg.L-1) and BAP (0, 1, 2, and 3 mg.L-1) using Murashige and Skoog (MS) medium supplied with 25 ml.L-1 coconut water. The three basil species responded differently to NAA and BAP in the media. The optimum concentration of NAA and BAP to induce callus growth in Lemon Basil was 0.5 mg.L-1 and 3 mg.L-1, respectively, with BAP alone showing the best effect on proliferation. In “Greek Minette”, however, the addition of NAA and BAP had no significant effect on the callus growth, proliferation, height, and number of leaf, node and root per explant. Media without growth regulators provide the best results in increasing height, explant proliferation rate, number of nodes and shoots, and percentage of proliferating shoots of “Thai Siam Queen”.Keywords: cytokinin, “Greek Minette”, Lemon Basil, “Thai Siam Queen”, synthetic auxin

Photoautotrophic System: A Review and Potential Application for Plant Propagation In Vitro Krisantini, Krisantini; Wiendi, Ni Made Armini
Journal of Tropical Crop Science Vol 5 No 2 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

AbstractThe standard method of in vitro plant micro propagation uses of tightly closed culture bottles using agar media containing macro and micro nutrients and sucrose as a source of carbon for the explants. The closed bottle culture is usually kept in a temperature and light controlled environment which is lower and of different quality from the natural sunlight, resulting in high relative humidity and no air exchange inside the bottles. Explants produced in vitro have malfunctioned stomata, undeveloped cuticles and lower leaf chlorophyll levels, and hyper hydration of the plantlets. Photoautotrophic tissue culture is micro propagation without or with a reduced sugar level in the culture media, so the growth or accumulation of carbohydrates of the explants is dependent fully upon photosynthesis and inorganic nutrient uptake. This method is usually combined with ventilation or CO2 enrichment, and recently, with incorporating porous materials such as vermiculite, gum or paper pulp to the agar media to promote better root system of the explants. This article discuss the advantages and disadvantages of the photoautotrophic micro propagation compared to the standard micro propagation methods, and provided the results of the photo autotrophic micro propagation studies conducted at Laboratory of Tissue Culture II of the Department of Agronomy and Horticulture, Bogor Agricultural University, Indonesia.

In Vitro Polyploidy Induction of Patchouli (Pogostemon Cablin Benth.) by Colchicine Afifah, Ulil Azmi Nurlaili; Wiendi, Ni Made Armini; Maharijaya, Awang
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Patchouli (Pogostemon cablin Benth.; 2n = 32) is an aromatic herbaceous plant commonly cultivated for use in the the fragrance industry. As patchouli is propagated by cuttings polyploidization induction by colchicine treatment was conducted to obtain a new genotype with high patchouli alcohol content. This research aimed to increase patchouli genetic diversity with colchicine treatment by the formation of a polyploid plant. Axillary buds from single node cutting of aseptic plantlets were used as the explants. The experiment was arranged using a factorial completely randomized design with two factors, namely the concentration of colchicine and the immersion duration. Genetic diversity of patchouli was successfully enhanced by adjusting the colchicine concentration and immersion treatment. lethal concentration (LC) of 50% in patchouli was 0.132% and the LC 50% for soaking time was 60.16 hours. The number of chloroplasts, stomatal length and chromosome number increased with increasing ploidy, whereas stomatal density and the number of trichomes decreased. The chromosome number of 16 patchouli mutants in generation 4 was still unstable, and a chimera was found with mixoploidy between 20-80. A hierarchical dendogram clustered 16 patchouli mutants into four different groups.

Increasing Ploidy Level of Garlic (Allium sativum L.) “Tawangmangu Baru” In-Vitro Using Colchicine Hailu, Molla Gebreyohannes; Wiendi, Ni Made Armini; Dinarti, Diny
Journal of Tropical Crop Science Vol 7 No 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.122-136

“Tawangmangu Baru” garlic variety is known to have low productivity. The variety is still highly demanded due to its strong flavour and aroma; however, its production has not yet been able to fulfill the local needs of Central Java due to the small size and limited production area. This study aimed to determine the effect of concentration and time duration of colchicine treatment towards increasing the ploidy level of “Tawangmangu Baru” garlic variety for genetic variability. The experimental design used in this study was a complete randomized design with two factorials and 12 combinations. The first factor was concentration of colchicine, i.e. 0.00, 0.02, 0.04, 0.06, 0.08 and 0.10%, and the second factor was the immersion time, i.e. 24 and 48 hours. The result indicated that, 4.72% callus induction was obtained in BDS + 0.4 mg.L-1 2,4-D + 2.0 mg.L-1 kinetin; and 4.0% callus proliferation were obtained in both BDS + 1.5 mg.L-1 2,4-D + 1.0 mg.L-1 kinetin and MS +1.5 mg.L-1 2,4-D and 1.0 mg.L-1 kinetin. The untreated plantlets showed higher mortality rate compared to the explants with 48 hours colchicine treatment. Higher number of shoots were recorded in 0.1% colchicine at 48 hours and lower shoots in 24 hours, whereas 0.1% colchicine at 24 and 48 hours showed the highest ploidy level of total nuclear DNA analyzed by flow cytometry. The genetic diversity of the “Tawangmangu Baru” garlic was successfully enhanced by colchicine and immersion treatment. Mutant lines with tetraploid and mixoploid plants were obtained. The putative lines obtained at 0.1% colchicine treatment were subcultured to produce new mutants before testing the phenotype. The application of colchicine at 24 and 48 hours treatment improved the genetic potential of “Tawangmangu Baru” garlic variety in vitro. The application of colchicine increased the ploidy level and an increase in ploidy is expected to make the bulb size larger. Larger tuber size will increase the tuber weight, and also the overall garlic productivity and production in the future.

Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri , Nurhasanah; G. A. Wattimena; Agus Purwito; Ni Made Armini Wiendi; , Suharsono
Indonesian Journal of Agronomy Vol. 31 No. 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Hordothionins are small anti-bacteria proteins present in barley endosperm, To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Agrobacterium tumefaciens strain LBA 4404, under the control of a cauliflower mosaic virus (CaMV) 358 promotor. The in vitro grown stem (internodus) was used in this research. After 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesific primer from CaMV 358 promotor showed the presence of amplified T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested, The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level, There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. Key words: Potato, Hordothionin gene, Disease resistance

PERBANYAKAN IN VITRO TANAMAN BAWANG PUTIH (Allium sativum L.) VARIETAS LUMBU PUTIH MELALUI INDUKSI TUNAS ADVENTIF Ni Made Armini Wiendi; G. A. Wattimena; Enny Prasetyanti
Indonesian Journal of Agronomy Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Two sets of experiments were conducted to determine the effect of growth hormones, argynine, and coconut water, on the adventitious shoot induction from garlic tissue, and also to find out the best medium for adventitious shoots proliferation. Both experiments could induce direct adventitious shoot and indirect adventitious shoot formation, from calli. Medium with 2 ppm Kinetin and 0.4ppm 2,4-Dproduce good quantity and quality of shoots. The number of shoot from this medium were 32.6 shoots per explant. Medium with 0.5 ppm Kinetin, 0.1 ppm 2.4-D, 25 ppm Argynine, and 10% coconut water produce the highest diameter and good quality of calli, while medium with 1 ppm 2iP and 25 ppm Argynine induced adventitious shoot from calli and produced the highest number of shoot per culture (33.9 shoots).

Pembentukan Embrio Endospermik Sekunder Mangga (Mangifera indica L.) Gedong Gincu Klon 289 Irni Furnawanthi Hindaningrum; Ni Made Armini Wiendi; Winarso Drajad Widodo
Indonesian Journal of Agronomy Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe improvement of Mangifera indica L. by conventional breeding approaches has been confounded by the long generation cycle, low fruit set, single seed per fruit and high degree of cross pollination. Biotechnology complements conventional breeding and expedite the mango improvement programs. Endosperm culture is a direct method to produce triploid plants. This study aimed to obtain embryo from endosperm culture. The system of secondary somatic embriogenesis in mango described here represents a source of embryogenic material may be used for mass propagation and genetic manipulation of this crop. The method consisted of induction, proliferation, maturation, germination, and histological analysis of the obtaimed embryos. A protocol for plantlet regeneration was developed for Gedong Gincu mango clone 289 through secondary somatic embryogenesis. Primary somatic embryos (proembryo and cotyledonary embryos) were cultured in induction medium to induce the secondary somatic embryos. The best proliferation rate was 0.22 in medium with 1 g L-1 Poly Vinyl Pyrrolidone (PVP) for multiplication of secondary somatic embryos. Maturation of inoculum derived from the proliferation medium supplemented with 2 g L-1 of activated charcoal on medium containing 0.4 mg L-1 BAP provides the average 2.39 embryo formation of cotyledonari phase. The highest germination frequency (20%) was obtained in media with GA3 1.5 mg L-1.Keywords: endosperm, Gedong Gincu, Mangifera indica L, secondary endospermic embrio

Co-Authors , Krisantini , Nurhasanah , Suharsono Afifah, Ulil Azmi Nurlaili Agus Purwito Ajisyahputra, Nikko Rizky Amelia Paramitha Mahanani Awang Maharijaya Bagus Setyo Yudhanto Baidowi, Muhammad Bawonoadi, Gilar Constantin, Mondjeli dan Budi Winarto dan Nurita Toruan-Mathius dan Sudarsono Diny Dinarti Endah Retno Palupi Enny Prasetyanti Fitri Rachmawati G. A. Wattimena Gilar Bawonoadi Hailu, Molla Gebreyohannes Irni Furnawanthi Hindaningrum Joko Mulyono Lailatul Qodriyah Agne Verawati Masna Maya SINTA Muhammad Baidowi Musalamah Nurhajati Ansori Mattjik Putri, Devina Daratyama Rahmah, Syifaur Rai, Sonya Putri Sonya Putri Rai Sri Rianawati Syarifah Iis Aisyah Tubagus Kiki Kawakibi Azmi Wachyar, Ade Willy Bayuardi Suwarno Winarso D. Widodo Yudhanto, Bagus Setyo Yuni Fitri Cahyaningsih Zikril Illahi

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