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Journal : Hasanuddin Journal of Animal Science (HAJAS)

The Chaaracteristics Yeast Isolated from Commercial Kefir Grain, Indonesia Sulmiyati Sulmiyati; Nur Saleh Said; Deka Uli Fahrodi; Ratmawati Malaka; Fatma Maruddin
Hasanuddin Journal of Animal Science (HAJAS) Vol. 1 No. 1 (2019)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (821.492 KB) | DOI: 10.20956/hajas.v1i1.6519

Abstract

The objective of this study was to investigate yeast characteristics obtained from commercial grain kefir from Indonesian. Isolation based on macroscopic morphology and microscopic morphology. The first method of research is activation of kefir grain using 10% reconstitution milk, yeast growth on Potato Dextrose Agar (PDA-Agar) medium, yeast coloration by using Lactophenol cotton blue. Then, yeast identification was done by macroscopic and microscopic morphology. Macroscopic morphological observations are observations of colony morphology at the time of isolation and purification, including size, shape, texture, color, surface, elevation, and edges. Microscopic morphological observations include cell shape, budding (budding) which first make preparations with yeast coloring then observed with Zeiss Asio Imager A2 Microscope using Zeiss Axiocam HRC camera. Macroscopic observation of yeast size description colony very small, small, medium, large, colony form is round, the margin is raised, the elevation is entire, the texture is smooth and surface glistening, cream colony color, and yeast smell characteristic. Microscopic observation seen there is cell nucleus, oval, there is pseudohypa, budding, gram-positive, urea test negative, glucose, lactose, maltose fermentation test positive, sucrose fermentation test negative, growth test on liquid media growth in surface medium (pellicle), and bottom medium (sediment). Based on the morphological observations in macroscopic and microscopic yeast identified genus Saccharomyces.
Evaluation of Color, Hardness, and Tannin Content of Soft Cheese with Biduri Leaf Extract Level sulmiyati Natsir; Gemini Ermiani M. Malelak
Hasanuddin Journal of Animal Science (HAJAS) Vol. 4 No. 2 (2022)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v4i2.21903

Abstract

Soft cheese Suspesi is a soft cheese to which biduri leaf extract is added as a coagulant. There is no research on the color, hardness, and content of tannins contained in soft cheese suspesi. The purpose of this research was to analyze and evaluate the hardness, color *L, *a, *b, and tannin content with the addition of biduri leaf extract at levels of 3% and 5% in soft cheese suspesi. This research used a completely randomized design with two treatments with six replications. A1= Biduri leaf extract level 3% (v/v), A2= Biduri leaf extract level 5% (v/v). Data on hardness, color, and tannin content were analyzed by Independent T-Test. The results showed a significant difference (P<0.05) in the hardness, color *L, and tannin content. Color *a and color *b did not show a significant difference (P>0.05). In conclusion, the levels of added biduri leaf extract gave different levels of hardness, color *L, *a, *b, and tannin content in the soft cheese suspesi. The characteristics of the soft cheese suspesi increased in terms of hardness, color *L, *a, *b, and tannin content in line with the level of added biduri leaf extract.