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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
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Articles 181 Documents
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The Effect of Seed Coating with Trichoderma sp. and Application of Bokashi Fertilizer to the Quality of Soybean (Glycine max. L) Seed Sarah Sakinah Umadi; Sumadi Sumadi; Denny Sobardini Sobarna
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3204

Abstract

The decrease of soybean productivity was caused by low quality of seed. To improve the quality of seed, soybean seed were coated  with Trichoderma sp. and adding bokashi organic fertilizer. This research aimed at finding the best dose combination of Trichoderma sp.  and bokashi fertilizer to improve the quality of soybeans. The research was conducted in the experiment field and Laboratory of Seed Technology at Padjadjaran University in April - August 2017. The experimental design in this research was Randomized Block Design (RBD). The treatments were the combination  of four dose values of coating the seed by Trichoderma sp. (0g/100 seeds, 1g/100 seeds, 2g/100 seeds, and 3g/100 seeds) and three dose  of bokashi (0g/polybag, 300g/polybag, and 600g/polybag) and each treatment was replicated three times. The experiment result showed that all treatments on soybean seeds did not affect the germination percentange, vigor index, and conductivity value, but affected the seed quantityof 600g/polybag of bokashi and without seed coating with Trichoderma sp.  on 100 seed mass and seed weight per plant.
Growth of Rice (Oryza sativa) Varieties: Mendawak, Inpari 34, Ciherang, and Bangir in Ciganjeng Village, Pangandaran District Muhamad Khais Prayoga; Neni Rostini; Tualar Simarmata; Mieke Rochimi Setiawati; Silke Stoeber; Kustiwa Adinata
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3232

Abstract

Type of rice varieties is one of the important factors that affecting rice production. For countries, rice breeders of Indonesia can take advantage of specific environmental potential in determining the distribution policy of superior varieties. The purpose of this study was to observe the appearance of plant height and the number of tillers of four rice varieties in Ciganjeng Village, namely Mendawak, Inpari 34, Ciherang, and Bangir. The design used completely randomized block design and repeated three times. Observations were carried out eight times in the vegetative phase, started from 14 days to 63 days after planting. This research was participatory and involved farmers. Farmers roled as observers to measure and record observation. All data analyzed by ANOVA with tukey’s HSD test as post hoc test. The results showed that both Inpari 34 and Bangir variety had the highest average plant height growth while Mendawak and Bangir has the highest average number of tillers.
Antibakteri dari Bulu Babi (Diadema setosum) Terhadap Escherichia coli dan Staphylococcus aureus Ida Indrawati; Tri Rahayu Hidayat; Nia Rossiana
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.2410

Abstract

Bulu babi (Diadema setosum) merupakan biota perairan yang memiliki nilai gizi tinggi. Senyawa bioaktif yang dihasilkan oleh bulu babi memiliki potensi untuk dimanfaatkan sebagai senyawa antibakteri alami. Penelitian ini bertujuan untuk mendapatkan aktivitas antibakteri dari ekstrak duri, badan dan gonad Bulu Babi. Metode penelitian yaitu metode eksploratif dan dianalisis secara deskriptif, melalui tahapan ekstraksi dengan pelarut etanol dan uji sensitivitas dengan metode difusi Kirby-bauer. Bulu babi diperoleh dari Pantai Barat Pangandaran, Jawa Barat, Indonesia. Dari ketiga ekstrak bulu babi, ekstrak gonad memiliki aktivitas antibakteri tertinggi pada konsentrasi 80% dengan zona hambat 8,5 mm terhadap bakteri Escherichia coli dan konsentrasi 80% dengan zona hambat 14 mm terhadap bakteri Staphylococcus aureus, dan didapatkan 3 isolat  bakteri dari duri bulu babi dan isolat  yang didapatkan adalah koloni putih kekuningan termasuk basil gram positif, koloni putih termasuk basil gram positif dan basil gram negatif serta koloni kekuningan yang termasuk basil pendek gram positif. Isolat 1 lebih efektif  terhadap S. aureus dengan zona hambat 11 mm. Isolat 2 lebih efektif terhadap E. coli dengan zona hambat 11 mm. Isolat 3 memiliki zona hambat yang hampir sama antara kedua bakteri uji.
Uji Aktivitas Bakteriofage Litik dari Limbah Rumah Tangga Terhadap Salmonella Typhi Yoga Dwi Jatmiko; Agung Putra Purwanto; Tri Ardyati
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3471

Abstract

Salmonella Typhi merupakan salah satu bakteri yang menjadi agen penyakit bawaan makanan. Bakteriofage sebagai alternatif penggunaan antibiotika telah digunakan untuk mengendalikan bakteri tersebut. Penelitian ini dilakukan untuk mendapatkan isolat bakteriofage litik yang mampu melisis beberapa bakteri patogen yang diujikan dan mengetahui pengaruh aktivitas bakteriofage litik terhadap pertumbuhan SalmonellaTyphi. Bakteriofage diisolasi dari limbah rumah tangga. Selanjutnya penentuan host range bakteriofage terhadap bakteri patogen lain dilakukan dengan metode spot test. Uji aktivitas bakteriofage terhadap SalmonellaTyphi dilakukan menggunakan metode bacterial challenge test. Berdasarkan hasil isolasi, didapat enam isolat bakteriofage, yaitu B2-St, B3-St, S1-St, S2-St, SL1-St, dan SL3-St. Semua isolat bakteriofage mampu melisiskan sel bakteri Escherichia coli dan Salmonella Typhimurium namun tidak mampu melisiskan Bacillus cereus, Staphylococcus aureus dan Shigella disentriae.Tiga isolat bakteriofagetelah terpilih berdasarkan densitas plaque terbanyak yaitu B2-St, SL3-St dan S2-St. Kemampuan isolat bakteriofage B2-St dalam melisiskan sel Salmonella Typhi lebih tinggi (6,81 ± 0,35 log sel/mL) daripada isolat bakteriofage SL3-St (7,39 ± 0,31 log sel/mL) dan S2-St (7,60 ± 0,27 log sel/mL). Penurunan densitas sel inang terendah oleh ketiga isolat bakteriofage terjadi pada jam ke-4. Bakteriofage B2-St merupakan bakteriofage terbaik dan berpotensi sebagai agen biokontrol Salmonella  Typhi. 
Pengaruh Ekstrak Etanol Daun Sambiloto Terhadap Jumlah dan Motilitas Spermatozoa Mencit Jantan Susilo Susilo; Budhi Akbar; Ika Pratinaningsih
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3505

Abstract

Tanaman sambiloto (Andrographis paniculata Ness.) di Indonesia dikenal sebagai salah satu tanaman yang berkhasiat sebagai obat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun sambiloto terhadap jumlah dan motilitas spermaozoa mencit (Mus musculus L.) jantan galur DDY. Sebanyak 24 ekor mencit jantan dengan berat antara 100-125 g yang dibagi menjadi empat kelompok perlakuan, diberikan ekstrak etanol secara oral selama 15 hari. Kelompok mencit pada perlakuan satu (P1) diberikan ekstrak etanol daun sambiloto 84 mg/kg bb. Kelompok mencit pada perlakuan dua (P2) dan tiga (P3) masing-masing diberikan ekstrak etanol daun sambiloto 140 mg/kg bb dan 196 mg/kg bb. Sedangkan kelompok kontrol (P0) tidak diberi perlakuan. Hasil penelitian menunjukkan bahwa pada kelompok perlakuan P3 (196 mg/kg bb) menunjukkan jumlah spermatozoa terbanyak yaitu 7,33 juta/ml, mengalami penurunan sebesar 78,86% terhadap kontrol.  Kandungan senyawa andrografolid dan flavonoid pada ekstrak sambiloto diduga kuat menjadi penyebab penurunan jumlah dan motilitas spermatozoa mencit. Hasil penelitian ini memberikan informasi bahwa pemberian ekstrak etanol daun sambiloto berpengaruh terhadap jumlah dan motilitas spermatozoa mencit jantan.
Condition of Food Sanitation and Hygiene in East Bandung, West Java Epa Paujiah; Maratus Solikha; Yani Suryani
Jurnal Biodjati Vol 3, No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3470

Abstract

Food security is one of the health problems in Indonesia which is related to the lack of knowledge of how the production process, the hygiene and health of the food. This study aims to describe the sanitary and hygienic conditions of food sold by merchant in Cibiru, Bandung City, West Java. The study was conducted in May-June 2017 by determining five sampling location based on the number of merchant in Cibiru. Data was obtained through questionnaire filling of 40 respondents at each sampling point which was then analyzed to find out the characteristics of social-demographic of the respondents, personal hygiene, and support of environmental sanitation. The results shows that the sanitation and hygiene of food in the aspect of knowledge of personal hygiene showed that the food handler was experienced food processors (88%) and 12% had no experience even though their last education was not linear with their trading profession. In addition, respondents' knowledge of individual hygiene shows that out of the nine aspects observed, 33% are in the high category, 11% are moderate and 56% are in the low category. Aspects of environmental sanitation and other support of food sanitation show that the conditions are in the clean and adequate category. All of these aspects can be used as a basic consideration in further counseling about food hygiene and food processing so that it can avoid unpredictable outbreaks.
The Capability of Consortium Phosphate Solubilizing Bacteria and IAA Producing Fungi on Promoting Elephant Grass Growth Witiyasti Imaningsih; Anang Kadarsah; Raden Dwi Thriwantho Rusmannurrachmad
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4284

Abstract

 Phosphorus compound in the critical land is relatively low that caused phosphorus is bonded to metals. The isolate BRC-AP and BRC-AK are a phosphate solubilizing bacteria (PSB) indigenous critical land that has the ability to dissolve phosphate. The used of phosphate solubilizing bacteria are able to improve plant growth on critical lands. The aimed of this study was to measure the growth rate of shoot length, root length and biomass of root, and leaf cuttings elephant grass (Pennisetum purpureum cv. King Grass) which inocu-lated with the consortium of phosphate solubilizing bacteria and IAA producing mold. Phosphate dissolving activity is determined by the formation of clear zone in solid Pikovskaya medium, synergism assay between bacteria and fungi observed on SDA medium then proceed testing the ability of bacteria and fungi consortium to promote plant growth. The result showed that the highest of phosphate solubility in-dex was found in isolate BRC-AP (27), addition of the combination of isolate BRC-AP, BRC-AK and Humicola sp. R.Dn have significant effect on shoot growth in the first week and leaf dry weight. The high-est length shoot of cutting elephant grass was found in treatment with the consortium of phosphate solubilizing bacteria and IAA producing mold (BRC-AP + BRC-AK + KP) (58.66 cm). The highest of leaf dry weight was found in treatment with BRC-AP + BRC-AK + KP (5.65 gr). The addition of consortium phosphate solubilizing bacteria and IAA producing fungi from South Kalimantan critical land proved can improve plant growth especially length shoot and leaf dry weight.
The Role of Endothelial Microparticle in Coronary Heart Disease as The Complications of Diabetes Mellitus Eka Fithra Elfi; Yose Ramda Ilhami; Eryati Darwin
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4164

Abstract

  Coronary heart disease (CHD) is caused by obstruction of coronary blood flow due to endothelial dysfunction triggered by various genetic and non-genetic risk factors such as hyperlipidemia, hypertension, hyperglycemia and obesity. Endothelial cell activation due to hyperglycaemia in diabetes mellitus induces production of pro-inflammatory factors that damage the cell membrane triggering the formation of membrane particles called microparticles. Endothe-lial microparticles contain proteins including endothelial nitric oxide synthase (eNOS) which plays a role in the production of nitric oxide (NO). To determine the role of microparticles in the occurrence of coro-nary heart disease in diabetes mellitus due to endothelial dysfunction, a study was conducted by comparing the levels of eNOS and NO in DM patients who had CHD with DM patients who had no CHD. Blood samples from 20 DM patients who had CHD and 20 DM patients who had no CHD of the outpatients in Cardiology Department and Inter-nal Medicine department of regional public hospital were included in this study. All patients were fulfilled inclusion and exclusion criteria and diagnosed by the appropriate specialist. The eNOS and NO lev-els were measured using the ELISA method. The results of this study show that eNOS levels in the group of DM patients who had CHD (21,292±12,415 ng/ml) were significantly lower (p <0.05) than those in the group of DM patients who had no CHD (29,721±11,952 ng/ml). Nitric oxide levels in DM patients who had CHD (0,053±0,021 nmol/ μl) were not statistically different to the levels in DM patients who had no CHD (0,047±0,032 nmol/μl). From the results of this study we concluded that endothelial microparticle protein eNOS plays a role in the occurrence of CHD due to the complications of diabetes mellitus 
Prevalence and Intensity of Ectoparasites on Clarias gariepinus From Aquaculture Pond in Aceh Besar District, Indonesia Fitria Nelda Fautama; Ilham Zulfahmi; Muliari Muliari; Adian Aristia Anas
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4328

Abstract

Information related to the prevalence and intensity of para-sites in an aquatic environment is crucial as preventive and responsive efforts to manage aquatic resources, especially for fish farming pur-poses. Up to now, information related to the prevalence and intensity of ectoparasites on catfish from aquaculture ponds in Aceh Besar Dis-trict is rare. This study aims to investigate the prevalence, intensity, and dominance of ectoparasites on catfish from aquaculture ponds in Aceh Besar district. The total of 200 catfish from four research stations at Sibreh, Kareung, Lambaro, and Limpok was observed in this study. The ectoparasites observation was focused on the skin, fins, and gill of the catfish. The observation parameter consists of prevalence, in-tensity, domination, length-weight relationship, and condition factor. Identification result showed that there were four types of ectoparasites infect the catfish in the aquaculture ponds in Aceh Besar District i.e Trichodina sp., Gyrodactylus sp., Dactylogyrus sp. and Ichthyopthyri-us multifiliis. Station 3 (Lambaro) had the highest prevalence and in-tensity, which were 64% and 5.87 ectoparasites/fish, respectively. In-fected catfish tended to have a lower weight growth than healthy catfish.
Non-Destructive Classification of Fruits Based on Vis-nir Spectroscopy and Principal Component Analysis Kusumiyati Kusumiyati; Yuda Hadiwijaya; Ine Elisa Putri
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4389

Abstract

Fruits are one of the sources of nutrition needed for health. Fruit quality is generally assessed by physical and chemical properties. Measurement of fruit internal quality is usually done by destructive techniques. Ultraviolet, visible and near-infrared (UV-Vis-NIR) spec-troscopy is a non-destructive technique to measure fruit quality. This technique can rapidly measure the fruit quality, the measured fruit still remains intact, and can be marketed. Besides, UV-Vis-NIR spectrosco-py can also be used to classify fruits. The study aimed to classify var-ious types of fruits using UV-Vis-NIR spectroscopy with wavelengths of 300-1041 nm and Principal Component Analysis (PCA). First de-rivative savitzky-golay with 9 smoothing points (dg1) and multiplica-tive scatter correction (MSC) were applied to correct the spectra. The results showed that the use of uv-vis-nir spectroscopy and PCA com-bined with spectra pre-treatment of the MSC method were able to clas-sify various types of fruits with 100% success rate in all fruit samples including sapodilla, ridge gourd, mango, guava, apple and zucchini. 

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