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Medical Laboratory Analysis and Sciences Journal

Melysa
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Published by Sekolah Tinggi Ilmu Kesehatan Hutama Abdi Husada Tulungagung

ISSN : 2656937X EISSN : 26571471 DOI : -

Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

The Medical Laboratory Analysis and Sciences Journal (Melysa) particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and also any sciences that cover sciences of medical laboratory area.

Arjuna Subject : -

Articles 26 Documents

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Identification of formalin and borax on tuna in Ngemplak market Tulungagung regency Qurrotu A’yunin Lathifah; Dora Dayu Rahma Turista; Ludfiana Azizah; Ahmad Erfan Khulaifi
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Misuse of formalin and borax is still common, including processing and preserving fish. Therefore, this study aims to identify the presence of formalin and borax in tuna on the market Ngemplak, Tulungagung regency. The samples used in this study were fresh tuna in the Ngemplak market originating from the beaches of Prigi, Sine, Tambakrejo, Popoh and Sidem. The samples were tested qualitatively using the colorimetric method by Schryver reagent for formalin and curcumin paper for borax. The results obtained in this study, from the five tuna samples not detected contained formalin and borax which were marked by no change in color in the test. Tuna sold in the Ngemplak market Tulungagung regency negatively contains formalin and borax, making it safe for consumption. It is hoped that the relevant office will carry out routine checks on the misuse of food additives, especially preservatives circulating in the community and the public should always be careful in choosing safe food.

Analysis of bulk oil in Tulungagung district on physical and chemical properties Taufik Salis Syaifudin; Rizqi Asri Fauzi Nugraha; Indra Lasmana Tarigan
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

This study aims to analyze the quality of Bulk frying oil in the Tulungagung district. Four samples were a test using physical parameters, organoleptic, density, and viscosity, while the chemical parameters were a test or the value of free fatty acids, acid numbers, and peroxide numbers. Comparison of the results of the analysis with SNI (Indonesian Standard Oil Quality), the four samples have poor chemical properties quality, it could be known by the acid number of samples 0.56, 0.78, 0.78, 1.65 (%) and Free fatty acids number 2.86, 9.98, 4.23, 6.7 (%) are higher than SNI 0.3 and 0.6 (%), even sample D has a poor peroxide number. So that overall, it is shown that the quality of Bulk oil from the samples used is not good enough.

Blood calcium levels in factory workers who consume supplements drinks Chalies Diah Pratiwi; Hariyanto Hariyanto; Amelia Rahma Perdana Putri
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Energy supplement drinks are drinks that contain caffeine, taurine, sweeteners, and other substances that have the effect of increasing energy, concentration, alertness, maintaining physical strength, and reducing drowsiness. Blood calcium is an important mineral that the body needs. The purpose of this study was to determine blood calcium levels in marble factory workers in Besole Village, Tulungagung Regency. The research design used was Analytical Descriptive. The population in this study were all marble factory workers who routinely consumed energy supplement drinks in the village of Besole in 2018 using a total sampling technique of 30 respondents. This research was conducted at the Laboratory of Dr. Iskak Tulungagung in May 2018 using the O-CPC method. The results obtained from the examination of blood calcium in marble factory workers showed 10 respondents (33%) had blood calcium levels within normal limits, 17 respondents (57%) had deep blood calcium levels. low limit and 3 respondents (10%) had very low blood calcium levels. Consumption of energy supplement drinks that exceed the threshold can damage the kidneys and affect blood calcium levels because the caffeine content in energy supplements can increase the release of calcium in the bones so that more calcium is released through the urine.

Low density lipoprotein (LDL) in type 2 diabetes mellitus Andyanita Hanif Hermawati; Eka Puspitasari; Cholila Lailatul Nurmala
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Hyperglycemia due to abnormal insulin secretion, insulin work or both. Type 2 Diabetes Mellitus is the most among other types of diabetes mellitus. Diabetes mellitus is usually accompanied by dyslipidemia. Dyslipidemia is a disorder of lipid metabolism or cholesterol characterized by an increase or decrease in lipid fraction in the plasma. Diabetes mellitus and elevated Low-Density Lipoprotein (LDL) cholesterol levels are often found to be interrelated. The purpose of this study was to describe Low-Density Lipoprotein (LDL) levels in patients with type 2 diabetes mellitus. The research design used was descriptive non-analytic. The population in this study were all patients with type 2 diabetes mellitus using a purposive sampling technique of 30 samples. Data collection was carried out by observing the medical record data of type 2 diabetes mellitus patients with Low-Density Lipoprotein (LDL) profile data for 2017. The results showed that patients with type 2 Diabetes Mellitus suffered the most by male sex with a percentage of 57%, aged over 45 years with a percentage of 97%, and having high LDL levels as much as 40%.

Analysis quality characteristics of virgin coconut oil (VCO): comparisons with cooking coconut oil (CCO) Dewi Natalia R; Ayu Natalia; Fendri Lukmanto; Ika Ani; Indra Lasmana Tarigan
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

This study aims to examine the quality of cooking coconut oil (CCO) and virgin coconut oil (VCO). The analysis was carried out as a physical parameter test including organoleptic and density while the chemical parameter test was: free fatty acid (FFA) peroxide number and test of saponification number. The sample oil used comes from coconut oil made traditionally by virgin coconut oil or commonly abbreviated as VCO, which is coconut oil which passes through a different extraction process with ordinary coconut oil so it is believed to have higher quality and provide more benefits. Samples derived from coconut oil traditionally processed in Beji Village, Boyolangu District with the brand "MALIKA". It was found that both oils had relatively the same physical parameters, the difference in density on VCO and CCO: 0.88 g / ml and 0.82 g / ml. As for the chemical parameters of free fatty acids, peroxide numbers, and saponification numbers of the VCO and CCO comparisons: 0.165: 0.207; 0.54: 0.68; 203: 321, so that the overall VCO has good quality, while CCO has an unfavorable saponification number.

The correlation between creatinine serum with total iron binding capacity (TIBC) on patients with sufferers chronic renal disease in Gambiran regional public hospital Kediri city Nala Fidarotul Ulya; Gilang Kusdinar; Kurniawan Santoso
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Chronic kidney disease (CKD) is kidney damage that occurs for more than 3 months with a glomerular filtration rate of less than 60 ml/sec/ 1.73 m2. WHO estimates that in Indonesia, there will be an increase in PGK in 1995-2025 by 41.4%. Over the past 40 years, Creatinine serum has become the most common and cheap serum marker for kidney function. One of the most common complications in patients with PGK is anemia. Anemia in PGK can be caused by several factors such as EPO deficiency, iron deficiency, etc. One of the usual parameters checking is the iron status of TIBC. This study aims to determine the relationship of serum Creatinine level with TIBC in chronic kidney patients in Gambiran regional public hospital Kediri and sampling taken by quota sampling. The study used cross-sectional using Rank Spearman statistic test. This test is used for abnormally distributed data types. The measured variable was serum Creatinine level with TIBC. The results of this study indicate that the value of p = (0.000)> α = (0.05), so H1 rejected and H0 accepted, then there is no relationship between serum Creatinine levels with TIBC in patients with chronic kidney disease in Gambiran Hospital Kediri.

Antimicosis activity of chloroform fraction of ethanol extract Soursop Leaves (Annona muricata, L.) Elly Rustanti; Zenny Fatmawati
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.24

Fungal infections are often found in the community, some antifungal drugs have been less effective so it is necessary to look for new candidate drugs as antimycotic, one of the candidates is soursop leaf (Annona muricata, L.). Soursop leaves contain compounds alkaloids, polyphenols, flavonoids, triterpenoids that function as antifungals. The purpose of this study was to determine the antifungal activity of the n-hexane fraction of the Soursop ethanol extract of the fungus Candida albicans and determine the class of compounds that act as antimycotic. Soursop leaf was extracted by maceration method using ethanol 96% then fractionated by partition method using chloroform solvent. The chloroform fraction was tested for antifungal activity against the fungus Candida Albicans by diffusion method with a concentration variation of 10%, 20%, and 30%. then identified by UV-VIS and FTIR spectrophotometry to determine active compounds as antifungals. The results showed that the Soursop leaf chloroform fraction had the highest antifungal activity at a concentration of 30% with an inhibitory diameter of 25.4 mm which was categorized strong. Compounds that are suspected to have antifungal activity from the soursop leaf chloroform fraction are terpenoid compounds. The results of this study are expected to provide scientific information to the public about the benefits of soursop leaves as an alternative treatment for fungal infections caused by the fungus Candida Albicans.

The parasite density and erythrocyte sedimentation rate on patients with uncomplicated tropical Malaria In two community health centre of West Lombok Kadeq Novita Prajawanti; Siti Zaetun; Pancawati Ariami
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.25

Malaria infections are often associated with the activation of coagulation and fibrinolytic systems. An increase number of fibrinogen levels in severe malaria and the increase of fibrinogen also stimulated the increase of erythrocytes sedimentation rate. The aim of this study is to find out about the effects of high parasitemia to erythrocyte sedimentation rate in patients with uncomplicated tropical malaria. Samples are collected using the Purposive Samplings method. To determined the effect of parasite density to erythrocytes sedimentation rate (ESR) levels (mm/h), the data were analyzed using the Non-Parametric Kruskal Wallis test (α=0,05). From 8 samples, 2 subjects (25%) has ++ (2+) densities with 35 and 46 mm/h; 3 subjects (37,5%) has +++ (3+) densities with 10, 65 and 70 mm/h; and also 3 other subjects (37,5%) has ++++ (4+) densities with 21, 44, and 70 mm/h. The Non-parametric Kruskal Wallis test shows that p-value 0,932 >α, means there is no effect of parasitic density on ESR levels in patients with uncomplicated tropical malaria. Using the ESR as the only-main biomarker in assessing the severity of malaria is an inaccurate idea because the ESR is more likely a non-specific test, therefore another blood test is needed to establish a diagnosis of malaria severity.

Effectivenes of hepatitis B vaccination in STIKes Jenderal Achmad Yani Cimahi students Gina Khairinisa; Fini Ainun Qolbi Wasdili
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.27

Hepatitis B is an inflammatory disease of the liver caused due to an infection from the hepatitis B virus (HBV). This inflammatory liver disease can become chronic, causing liver cirrhosis, and then become cancerous. Hepatitis B vaccination process by injection. Hepatitis B antibodies can appear in response to Hepatitis B vaccination. The purpose of this study as an effort to decide the effectiveness of Hepatitis B vaccination by seeing whether active immunity has formed against infections caused by the Hepatitis B virus to Stikes Student Jenderal Ahmad Yani Cimahi. The research method used in this research is descriptive. Samples used in the study were 30 people who met the inclusion criteria. From the results of studies using the immunochromatographic method, all positive samples were found with hepatitis B antibodies. Based on the research can be concluded that the vaccination conducted on Stikes Jenderal Achmad Yani Cimahi has given effective results and has a protective titer to prevent hepatitis B virus infection that will attack his body.

Antimicrobial inhibition zone test of purple okra (Abelmoschus esculentus) extract on the growth of gram-negative bacteria Anggun Wulandari; Dian Kusuma Wardani
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.29

Okra (Abelmoschus esculentus) contains secondary metabolite compounds that can function as an antibacterial which can inhibit the growth of gram-negative bacteria. This study aims to determine the antimicrobial inhibition zone of purple varieties of okra extract on the growth of gram-negative bacteria (Escherichia coli). This research is an experimental study using a Completely Randomized Design (CRD) with a disk diffusion testing method. The object used was purple okra fruit extract at concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% with negative control of DMSO and positive control of chloramphenicol. Inhibition zone diameter measurements are indicated by clear areas, ie areas that are not overgrown with bacteria. The results showed that the most optimum inhibition zone of purple varieties of okra fruit extract was at a concentration of 40%, 50% and 60% with an average value of 6.67 mm.

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Home Page

OAI Link

Editorial Team

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Contact Name Dora Dayu rahma turista

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Journal Mail Official melysajournal@gmail.com

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Location Kab. tulungagung, Jawa timur INDONESIA

Abstract

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Contact Name
Dora Dayu rahma turista

Contact Email
melysajournal@gmail.com

Phone
-

Journal Mail Official
melysajournal@gmail.com

Editorial Address
-

Location
Kab. tulungagung,

Jawa timur

INDONESIA