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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol 51, No 1 (2019)" : 10 Documents clear

Correlation between micro-RNA-21 expression and inflammation cytokine in rabbits implanted with bare metal stent with the incidence of neo intimal hyperplasia Hariawan, Hariadi; Soesatyo, Marsetyawan HNE; Munawar, Muhammad; Ghozali, Ahmad
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

In-stent restenosis after stenting in vascular occurs secondary to the accumulation of smooth muscle cell and extracellular matrix. This condition is a major complication caused by the occurrence of neointimal hyperplasia (NIH). The study aimed to prove the role of miRNA-21 as a risk predictor of the NIH event in aorta of rabbits animal model who underwent bare metal (BM) type stent implantation and to know the miRNA-21 role in IL-6 and IL-8 expressions. This study was quasi experimental, coducted in Catheterization Laboratory Dr. Sardjito General Hospital Yogyakarta. Stent implantation was observed intravascular ultrasonography. Blood vessel which was already deployed by stent taken as sample for pathologic examination. Rabbit blood samples were collected on day 0, 7, and 28. Quantification miRNA-21 expression has been done with qPCR and ELISA approach. These 10 rabbits divided into two groups in which one group as control (without stent deployment, 4 models) and another group as intervention (with BM type stent deployment, 6 models). The increase in expression of miRNA-21 on the day 7 and day 28 in the intervention group compared to the control group. Neointimal hyperplasia increased in intervention group on day 7, and 28 were from mild grade to moderate, and severe respectively. In addition, levels of IL-6 and IL-8 increased on day 28 compared with on day 7. This study showed increase of miRNA-21 expression on day 7 and NIH rise from the mild to moderate. Furthermore, on day 28, that increases of miRNA-21 expression and NIH rose from to moderate to severe. The increase of miRNA-21 was also shown on day 7 and 28 followed by the increase of IL-6 and IL-8 levels.

Critical appraisal of neuropathic pain guidelines in Asia Rizaldy Taslim Pinzon; Rosa De Lima Renita Sanyasi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Neuropathic pain (NP) is type of chronic pain that is common and often difficult to treat. Clinicians may be guided by a number of published guidelines and algorithms for the management of neuropathic pain. It is important for every clinician to know the quality of guidelines. The availability of current guideline in Asian countries is not well understood. Critical appraisal of NP guidelines in Asia has not performed, yet. The aim of this study was to appraise the quality of pharmacological treatment from neuropathic pain guidelines in Asia. Systematic searches were conducted by using combination of keywords i.e NP, Asia, and guideline. Guidelines evaluation was using appraisal of guidelines for research & evaluation II (AGREE II) instrument. The result interpreted as (i) a strongly recommended for use in practice if most domains scored above 50%; (ii) recommended for use with some modification if most domains scored between 30% to 50%; or (iii) not recommended for use in practice if most domains scored below 30%. After matched to inclusion and exclusion criteria there were 5 guidelines left: a guideline from Philippines, China, South Korea, Malaysia and Middle East. All of guidelines are recommended for use with some modification. The guidelines mention that first line treatment for NP are tricyclic antidepressants, selective norepinephrine reuptake inhibitor (SNRI) and alpha 2-delta ligand calcium channel blocker (CCB). Most of the evidences come from peripheral NP. There were limited evidences for the treatment of central neuropathic pain (central post stroke pain and pain after spinal cord injury). All the existing guideline mention, that the first line treatment for neuropathic are the tricyclic antidepressants, SNRI and alpha 2-delta ligand CCB.

Effects of resistant starch of mixed tubers snacks on glucose metabolism, leptin, visceral fat and body mass index in type 2 diabetes mellitus (T2DM) Hidayat, Jenny; Sunarti, .; Mustofa, .; Sadewa, Ahmad Hamim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Resistant starch could lower blood glucose, decrease adipocyte in adipose tissue and affect satiety hormones such as leptin. Tubers and pumpkin have high content of resistant starch, but their effectiveness to type 2 diabetes mellitus (T2DM) has not been known clearly. This research was conducted to determine the effectiveness of snack consumption made from tubers and pumpkins to BMI, visceral fat, glucose and leptin levels in the blood of T2DM patients and the correlation between the variables. The research method was pre-post clinical trial. Sixteen T2DM patients were in treatment (RS) and control groups. Subjects in RS group were given snack twice daily for 4 weeks. After following wash out process for 4 weeks, the same subjects was continued as subjects’ control. Paired t-test and/or Wilcoxon-test was used to analyze the differences between values before and after treatment in the group and between groups. Pearson test was used to analyze the correlation of BMI, visceral fat, glucose and leptin level. The visceral fat was increased in RS group (p=0.04) after 4 weeks consuming snack but decrease in control group (p=0.04) without significant change of BMI. Leptin level was decreased (p=0.00) in RS group. Blood glucose significantly decreased (p=0.01) and leptin level increased slightly in control group. Comparing the RS and control group at the end of study, there were significantly different in the variation of visceral fat in the female groups (p=0.05) and leptin (p=0.05). Visceral fat correlated with BMI in the RS and control group. In conclusion, the mixed tubers and pumpkin snack decreased the leptin level but increased visceral fat.

Urinary tract infection in premature rupture of membrane (PROM): an academic hospital based study Rahman, Muhammad Nurhadi; Liligoly, Rivaldi D; Pangastuti, Nuring
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Premature rupture of membrane (PROM) and preterm PROM (PPROM) are commonly related with poor maternal and perinatal outcomes. Urinary tract infection (UTI) has been known as one of its risk factors. The aim of study was to ascertain the frequency and pattern of urinary symptoms as well as the risk factors for UTI in PROM and PPROM. A retrospective study was conducted at Dr. Sardjito General Hospital, Yogyakarta, Indonesia. All pregnancy cases with the history of PROM/PPROM from January to December 2015 were included. The research subjects were obtained from medical records, using the format of basic data collection to identify the risk factors of UTI in pregnancy. One hundred cases of complicated pregnancy with either PROM or PPROM were obtained. The mean of maternal age, gestational age, and birth weight were 28 ± 5.99 yr; 34.05 ± 4:28 wk; 2170.79 ± 835.447 g; respectively. Urinalysis was done in 58 patients. The prevalence of bacteriuria was 55.17%. Symptomatic vs. asymptomatic bacteriuria showed statistically significant differences (p

Comparative histochemical study of the kidney of six mammalian species Oghoverere, Abiokuta Blessing; Igho, Odokuma Emmanuel
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Comparative anatomy describes the similarities and differences in the anatomy of different species. Comparative anatomical studies do not only provide evidence for evolution but also indicate that various organisms originate from a common ancestor. They assist scientists in classifying these organisms based on similar characteristics of their anatomical structure. The aim of this study was to compare histochemical of the kidney of six mammalian species. The animals were grouped into three based on their dietary patterns i.e herbivores (Capra aegagrus and Bos taurus), carnivores (Canis lupus and Felis catus) and omnivores (Rattus norvegicus and Sus scrofa). Tissues were obtained from each of the studied species and preserved in 10% formal saline for one week until analysis. The tissues were then subsequently processed and stained using general and specific histochemical techniques. It was observed from this study all the mammalian kidneys consisted of similar histological features which explained that these studied mammals undergo the same evolutionary trend, adaptational changes and phylogenic relationship that exist between the kidneys of the studied species. Despite their differences in dietary patterns, the studied species have similar histological features with little observed variation. However, they have little differences in histochemical staining pattern for glycogen and glycoprotein but in reticulin they all stain intensely. This study has established a strong relationship in the histology of the kidney in six mammalian species.

Correlation between ratio of Nrf2/Keap1 and catalase gene expression in liver of hyperlipidemic rats after administration of 7-hydroxy-2-(4-hydroxy- 3-methoxyphenyl)-chromen-4-one Prasetyastuti, .; Rubi, Dianandha Septiana; Pramana, Abrory Agus Cahya; Sadewa, Ahmad Hamim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Hyperlipidemia results in excessive superoxide anion radicals that are the cause ofoxidative stress. Phytochemical compounds can reduce oxidative stress. The aim of thisstudy was to investigate the correlations between ratio of Nrf2/Keap1 and catalase geneexpression in livers of hyperlipidemic rats after administration of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one. Twenty-four Rattus norvegicus rats, aged 8 weeks andweighing an average of 200 g were randomly divided into 6 groups i.e. Group 1 wasnormal group (N), Group 2 was hyperlipidemic rats (HL), Group 3 was hyperlipidemicrats with simvastatin (HL+SV), and Groups 4-6 were hyperlipidemic rats with 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one doses 10 mg (HL+10), 30 mg (HL+30) or 90 mg/200 g BW (HL+90), respectively, administered orally by gavages. At the end ofthe study, the rats were euthanized and the livers were used to analyze the ratio of Nrf2/Keap 1 and catalase gene expression. Nrf2/Keap1 ratio and catalase gene expressionbetween groups were analyzed by Kruskal Wallis test. Spearman’s correlation test wasused to analyze the correlations between Nrf2/Keap1 ratio and catalase gene expression.The administration of 3 different doses of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one on hyperlipidemic rats increased catalase gene expression. There wasno correlation between ratio Nrf2/Keap1 and catalase gene expression. In conclusion,administration of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one can improvecatalase gene expression in hyperlipidemic rats. However, there is no correlation betweenthe ratio of Nrf2/Keap1 gene expression and the catalase gene expression.

The difference of nasal mucosal cytology features in gas station workers compared to non-gas station workers Rianto, Bambang Udji Djoko; Kurniawan, Linggawati; Sudarman, Kartono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The pollutants contained in an outdoor air environment for example gasoline vapors can cause epithelial inflammation, metaplasia, and dysplasia. This study aimed to determine the difference in cytological nasal mucosa between gas station workers and non-gas station workers. This research used a cross-sectional design. There were 80 samples with inclusion criteria: aged 20-50 years, worked more than 3 months, consisting of 40 gas station workers (exposed group) and 40 non-gas station workers (unexposed group) who did not use nasal drops in the last ten days. The exclusion criteria of both groups were: 1) patients with acute rhinitis, 2) had nasal trauma, 3) had nasal surgery, 4) consume alcohol, 5) history of allergic rhinitis, and 6) refused to participate in the study. All samples performed brushing at 1/3 anterior nose inferior turbinate and cytology examination. The statistical analyses used X2 tests. From the exposed group we found 18 (45%) with inflammation, 17 samples (42.5%) with metaplasia, and 9 samples (22.5%) with dysplasia, while in the unexposed group there were 10 (20.5%) with inflammation, 4 (10.0%) with metaplasia and 2 (5.0%) dysplasia. There were statistically significant differences in nasal mucosal cytology, particularly metaplasia (p: 0.001; RP: 6.65; 95% CI: 1.78-27.01) and dysplasia (p: 0.023; RP: 5.52; 95% CI: 1.22-32.10) between both group samples. It can be concluded that there are statistically significant differences involving metaplasia and dysplasia in nasal mucosa cytology features of gas station workers compared to non-gas station workers.

Association between sexual orientation and sexual contact with the incidence of human immunodeficiency virus (HIV) infection in Dr. Sardjito General Hospital, Yogyakarta Imtihani, Hajar; Pudjiati, Angela Satiti Retno; Luthfiandi, Mochammad Rifky; Susetiati, Devi Artami
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Anal intercourse has been known to have a high risk of human immunodeficiency virus (HIV) transmission. The objective of this study was to evaluate the relationship between sexual orientation and the mode of sexual contact with the incidence of HIV infection. This was observational analytic study with cross sectional design. Subjects were new male patient who visited sexually transmitted infection (IMS) clinics at Dr. Sardjito General Hospital, Yogyakarta during 2016. Data were obtained from medical records. The HIV status was established from HIV rapid test and enzyme-linked fluorescent assay (ELFA). Data were analyzed using descriptive test and Pearson’s chi-squared test with significance level of p< 0.05. Among 167 subjects, 47.91% were in the age group 17 - 25 years old. Forty subjects (24%) had HIV positive status. The majority of subjects were heterosexuals (111 subjects (66.47%)), 43 subjects (25.75%) were homosexuals, and 13 subjects (7.8%) were bisexuals. HIV positive subjects were more common in homosexual groups than were heterosexual and bisexual (p

Pathogenesis, evaluation, and recent management of diabetic foot ulcer Wijaya, Lorettha; Budiyanto, Arief; Astuti, Indwiani; Mustofa, .
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Diabetic foot ulcers (DFUs) are a major health problem as the number of patients continues to increase, are difficult to heal, require enormous management costs, and deteriorate the quality of life of patients, their families and societies. The pathogenesis of DFUs is complex. Most important factors that increase the risk of DFUs are peripheral neuropathy, foot deformities, frequent minor trauma, and peripheral arterial disease. Neuropeptides derangement, hypoxia, hyperglycemia, and infection act as the cause of chronicity of DFUs. Therefore, during the initial evaluation of DFU, patients need to be checked for their metabolic status, presence or absence of peripheral neuropathy, vascular insufficiency, foot deformities, and infection of the ulcer and its underlying bone. Then, DFUs are classified by the severity of vascular insufficiency, the depth of the wound, and the severity of the infection. This classification system helps clinicians to determine whether the patient needs to be hospitalized or amputated and helps to establish DFU management strategies. In the management of DFUs, adequate blood flow to the wound area should be achieved. Glycemic control and standard wound care should be encouraged. Standard wound care includes debridement, offloading, wound moisture balance with suitable dressing, edema control, and infection control. Education about preventive foot care should be taught to the patients and their families. As the pathogenesis and management of DFUs are complex, a multidisciplinary team consists of expert individuals in their respective fields should be involved.

The role of nickel contact allergy in nummular dermatitis in Indonesia Niken Indrastuti; Moh Hakimi; Marsetyawan HNE Soesatyo; Hardyanto Soebono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

In the recurrence of nummular dermatitis (ND) as a problem for patients, it is necessary to identify interferon-γ (IFN- γ), interleukin-4 (IL-4), interleukin-17 (IL-17), and stimulation of lymphocytes against nickel. This study aimed to investigate the role of nickel contact allergy in ND. Forty-two patients with ND were studied and 42 healthy subjects who were equal in age, sex and atopy history as control. All subjects underwent nickel skin patch test, detection of IFN-γ, IL-4, IL-17 in blood, and lymphocyte stimulation assays. To determine cut off point of the variables, receiver operator characteristic (ROC) curves were calculated. Bivariate and multivariate analyses were performed to measure the strength of association using odds ratio (ORs) and 95% confidence intervals (95% CI). Statistical analysis was performed using McNemar X2-square test and multiple conditional logistic regression. Nickel contact allergy was shown by nickel patch test (OR= 3.5; 95% CI = 1.09–14.60), stimulation index/SI (OR= 29; 95% CI = 4.81-1184.43), IFN-γ (OR= 4.25; 95% CI = 1.39–17.36). These results were supported after multivariate analysis with conditional logistic regression which showed nickel patch test (OR= 9.63; 95% CI= 1.02–109.38; p= 0.04), SI (OR= 42.19; 95% CI = 2.32–766.03; p= 0.01), IFN-γ (OR= 11.51; 95% CI = 1.08–122.63; p= 0.04). Nickel contact allergy is an important risk factor for ND. Patients with ND are recommended to be tested for nickel contact allergy.

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Nurhadiyahya

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nurhadiyahya@ugm.ac.id

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+6289672800034

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Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA