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Edy Kurnianto
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INDONESIA
Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Articles 11 Documents
Search results for , issue "Vol 42, No 3 (2017): September" : 11 Documents clear
Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats D. A. Lestari; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.135-146

Abstract

The objectives of study were to identify and assess the amino acid diversity of Cytochrome b (Cyt b) gene, genetic marker and characteristic of specific amino acid in Kacang and Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa Grade goat (EG) were purposively sampled. The genomic DNA was isolated by Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with CytbCapF and CytbCapR primers and was sequenced. The results showed that there were two specific amino acids that distinguish KG and EG goat with C. hircus and C. aegagrus and four specific amino acids that distinguish KG and EG goat with C. falconeri, but there were no specific amino acids can be used as a genetic marker to distinguish between Kacang and EG goat. In conclusion, specific amino acids in Cyt b gene can be used as a genetic marker among KG and EG goat with 3 goat others comparator.
Effect of sunflower oil supplementation as unsaturated fatty acid source on rumen fermentability and performance of lactating Garut ewes Khotijah, L.; Pandiangan, E. I.; Astuti, D. A.; Wiryawan, K. G.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.185-193

Abstract

The study was aimed to evaluate the increasing levels of ration energy, with fat-rich unsaturated fatty acids from sunflower oil on the nutrient intake, rumen fermentability and performance of ewes. The experiment used fifteen ewes of 1.5 years old, primiparous, caged individually with their lambs and conducted three treatments and five replications from a completely randomized designed. Those treatments included R1= ration with 0% sunflower oil addition, R2= ration with 4%sunflower oil addition, R3= ration with 6% sunflower oil addition. The variables consisted of dry matter intake, nutrients, ewes performances (birth weight, weaning weight, decreased of body weight), total and partial VFA, the ratio of acetate: propionate, methane production, protozoa and bacterial population. Data were analyzed statistically and descriptively. The results showed that the treatments did not influence dry matter intake and nutrients,. rumen fermentability and ewes performances. Although the treatments increased (P<0.01) fatconsumption and reduced shrinkage of body weight. It can be concluded that the adding sunflower oil as the resource of unsaturated fatty acids up to 6% of the ration was not negatively affected the fermentability of rumen, moreover can improve ewes body condition and, accelerated body weight recovery
Comparative performance of Landrace, Yorkshire and Duroc breeds of swine Zebua, C. K. N.; Muladno, M.; Siagian, P. H.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.147-152

Abstract

The objectives of this study was to analyze the comparative ability of animal genetic, using evaluation of production that has important economic traits including Landrace, Yorkshire, and Duroc breeds of swine. A total of 49 swine consist 15 boars and 34 gilts, with average body weight of 18.50-21.50kg were used in this research. All of samples were raised in the same pen and feeding management. This research used the experimental method of Completely Randomized Design involving three treatments of animal breeds including Landrace, Yorkshire, and Duroc breeds. The data were analyzed by using analysis of covariance procedur General Linier Model and Least Square Means test. Boars and gilts were analyzed in separated data. The result showed that the effect of breed was significantly associated for all traits, but not for boars feed intake. The overall, Yorkshire and Landrace performed better than Duroc for average daily gain, feed conversion ratio, selection index, and age at 90 kg body weight. However, Duroc boars and gilts had the thinnest backfat followed by Yorkshire and Landrace.
Evaluation of feeding Indigofera zollingeriana leaf meal and Sardinella lemuru fish oil on lipids metabolism of local ducks Arini, N.M.J.; Sumiati, S.; Mutia, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.194-201

Abstract

The objective of this study was to evaluate the effectiveness usage of Indigofera zollingeriana leaf meal (I) and Sardinella lemuru fish oil (L) in diets on the decreasing levels of cholesterol and lipid metabolism of laying duck. One hundred and forty-four Magelang ducks aged 20-32 weeks, laying periode, with an average body weigth of 1,72±0,12 kg were used, which were divided into 6 groups of experimental diets, 3 replications, and of 8 layers each. A completely randomized design, 3x2 factorial design was used in this experiment. The first factor was usage Indigofera zollingeriana leaf meal which replaced protein soybean meal (0%, 5,5%, 11%). The second factor was usage Sardinella lemuru fish oil (0%, 2%). The results showed that the treatment (I5.5L2) was highly significant affected (P<0.01) the level of triglyceride, HDL, and LDL in the blood. The treatment I0L2 significantly decrease (P<0.05) total cholesterol of blood lipid profile and meat cholesterol. The treatment (I11L2) was highly significantly decreased (P<0.01) egg cholesterol. The conclusion of this study was that feeding Indigofera zollingeriana leaf meal 5.5% and Sardinella lemuru fish oil 2% in laying duck’s diet could maintain the balance of blood lipid profiles by lowering plasma triglycerides, LDL, cholesterol and raising up plasma HDL and plays an important role on lipid metabolism of Magelang laying ducks to produce eggs and meat with low cholesterol content.
Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle Agung, P. P.; Anwar, S.; Putra, W. P. B.; Zein, M. S. A.; Wulandari, A. S.; Said, S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.153-159

Abstract

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.
The use of coconut pulp as a feed substrate to methanogenesis inhibitor in in vitro rumen fluid fermentation Sondakh, E. H. B.; Kalele, J. A. D.; Ratulangi, F. S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.202-209

Abstract

This experiment was conducted to determine the effect of the adding of coconut pulp in ruminant feed on methane reduction in vitro. Rumen fluid and substrate of forage and concentrate in ratio 60:40 were in this study. This experiment consisted of five treatments of coconut pulp as followed: R1: feed substrates without the coconut pulp; R2: substrate feed with coconut pulp 5%; R3: substrate feed with coconut pulp 10%; R4: Substrate feed with coconut pulp 15% and R5: substrate feed with coconut pulp 20%. Each treatment was conducted to four replications. Fermentation was done using the Hohenheim Gas Test (HGT) incubated at 39oC for 72 hours. At the end of fermentation was determined production of methane, parameters of fermentation, microbial activity, feed digestibility and fatty acids profile of the ruminal fluid. Data obtained were analyzed by using analysis of variance using completely randomized design. The differences of mean values were analyzed by Duncan multiple range test (DMRT). The results showed that methane production has decreased about 13.74%-17.39% when the feed was given coconut dregs 15-20%. Based on the results of the study was concluded that the supplementation of coconut pulp up to 15-20% of 100% dry matter can be used as a substrate of ruminant feed, because it can reduce methane and does not interfere the microbial activity in the fermentation of rumen fluid through in vitro. The supplementation of coconut pulp can be a referencein designing the methanogenic inhibitor feed.
Breeding value of sires based on offspring weaning weight as a recommendation for selecting Kebumen Ongole Grade cattle Sumadi, S.; Fathoni, A.; Maharani, D.; Ngadiyono, N.; Widayati, D. T.; Noviandi, C. T.; Khusnudin, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.160-166

Abstract

The objective of study was to estimate breeding value of sires of Kebumen Ongole grade cattle based on offsprings weaning weight. This research was carried out from August to December 2015 in Urut Sewu area, Kebumen. The calculation of genetic parameter estimation was heritability of weaning weight. The value of heritability was used to calculate the breeding value of sires based on offsprings weaning weight. Heritability was calculated using paternal half-sib correlation with the direct pattern variance analysis. Breeding Value (BV) of sire was calculated by absolute estimated breeding value. The materials used in this study included were data record during the last three years (2013-2015) consisted of 41 sires, 51 dams and 244 calves. Weaning weight data had been corrected based on age of dams, sex and weaning age of 120 days. The results showed that the heritability value of weaning weight was included in high category (0.36 ± 0.21). The highest BV was KJ.J608 (49.76) with a ratio of 231.45%. The results of this study were expected to be the basis recommendation for selecting sires in Kebumen.
Back-matter (September 2017) Kurnianto, E
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Back matter of the Journal of the Indonesian Tropical Animal Agriculture, Vol 42, No 3 (2017).
Sexual dimorphism and identification of single nucleotide polymorphism of growth hormone gene in muscovy duck Ismoyowati, I.; Tugiyanti, E.; Mufti, M.; Purwantini, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.167-174

Abstract

This research was aimed to investigate the different growth and to identify growth hormone gene polymorphism in Muscovy ducks. Two hundred Muscovy day-old ducks consisting of white-plumed male and female duck, black and white-plumed male and female ducks. Body weight was recorded weekly and the obtained data were subject to T test. Primer design used the Custal X Program based on a database from the GeneBank Cairina moschata GH gene, partial cds (AB158762). Primer base sequence of GH gene was forward/Sequence: 5’-CTGGGGTTGTTTAGCTTGGA-3’ and reverse/Sequence: 5’-TAAACCTTCCCTGGCACAAC-3’. The DNA sequences were aligned by using the BioEdit version 7.7 for identification of the single nucleotide polymorphism. The result showed that male Muscovy duck produced higher an average body weight gain and more relative growth than those of females. The highest body weight gain was at three weeks old, and then it started to decrease at four weeks old. The sequencing PCR product obtained nucleotide polymorphism. AA genotype was observed at 136 t of black female Muscovy duck, CC in black and white male Muscovy duck, and white female Muscovy duck. Conclusively, a body weight gain of 3-week-old male Muscovy ducks was higher than that of females and GH gene polymorphism was observed in Muscovy ducks.
Front-matter (September 2017) Kurnianto, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

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Abstract

Front matter of the Journal of the Indonesian Tropical Animal Agriculture, Vol 42, No 3 (2017).

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