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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 19 Documents
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Search results for , issue "Vol 16 No 2 (2015)" : 19 Documents clear
Kemampuan Maturasi dan Fertilisasi Oosit Sapi yang Diseleksi Menggunakan Teknik Pewarnaan Brilliant Cresyl Blue (SELECTING MATURATION AND FERTILIZATION ABILITY OF BOVINE OOCYTES USING BRILLIANT CRESYL BLUE) Zultinur Muttaqin; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to evaluate the use of brilliantcresyl blue (BCB) in selecting potentialbovine oocytes for maturation and fertilization in vitro. Brilliant cresyl blue (BCB) is a dye that can assessintracellular activity of glucose-6-phosphate dehydrogenase (G6PD), a synthesized enzyme in maturedoocytes. Oocytes were exposed to 26 ?M BCB diluted in modified Phosphate Buffer Saline (mPBS, PBS +10% Fetal Bovine Serum) for 90 minutes is 5% CO2 incubator at 390C. The oocytes were classified accordingto their cytoplasm coloration: oocytes with blue cytoplasm (BCB+) and unstained oocytes (BCB-). Oocytesof the control group were incubated shortly after morphological selection without being exposed to BCB.Afterwards, all groups of oocytes (BCB+, BCB-, and control) were matured and fertilized in vitro. Maturedoocytes were those oocytes that reach metaphase II after 24 hours culturing; whereas oocytes showing twoor more pronuclei at 14 hours post incubation were classified as fertilized oocytes. The nuclear maturationrate was significantly (P<0.05) higher in BCB+ group (78.7% ± 0.41) than the BCB- group (33.3% ± 0.13).However, there was no significance difference (P>0.05) between the BCB+ and the control group (77.1% ±0.32). The fertilization rate was significantly higher (P<0.05) in the BCB+ group (30.5% ± 0.04) comparedto the BCB- (13.6%±0.03) and control group (23.6% ± 0.05). In conclusion, BCB staining of bovine oocytesprior in vitro maturation could be used in selecting potential developing oocytes.
Perkiraan Pasokan Nitrogen Mikrob pada Domba Ekor Tipis yang Diberi Bungkil Kedelai Terproteksi Tanin (ESTIMATION OF MICROBIAL NITROGEN SUPPLY IN THIN-TAILED SHEEP FED WITH TANNIN PROTECTED SOYBEAN MEAL) Husnaeni .; Sunarso .; Limbang Kustiawan Nuswantara
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was aimed to study the effect of soybean meal protection mangrove leaf tannin onpurine derivative (PD) excretion, estimated microbial nitrogen (MN) supply, and estimated efficiency MNsuplay based on DOMR in thin-tailed sheep. A total of 16 thin-tailed sheep aged eight months withaverage body weight of 11.81±1.65 kg, and were randomly fed with complete feed with tannin-protectedsoybean meal in different levels. The research was conducted according to completely randomized designwith four treatments and four replications. T0 (soybean meal without tannin), T1 (protected soybean meal0.5% tannin), T2 (protected soybean meal 1% tannin), and T3 (protected soybean meal 1.5% tannin).Research finding indicated that organic matter consumption (OMC), organic matter digestibility (OMD),and digestible organic matter in rumen (DOMR) were not affected (P> 0.05) by the treatment, however thetreatment had significant effects (P<0.05) against PD, estimated MN supply, and estimatedMN supply based on DOMR. Purine derivative, estimated MN supply, and estimated MN supply basedDOMR markedly decreased in the treatment of soybean meal protection 1.5% tannins. It was concludedthat soybean meal protection with mangrove leaf tannins was not significant against OMC, OMD andDOMR thin-tailed sheep, but protected soybean meal with tannins 1.5% affected on decreasing purinederivative excretion, estimated MN supply and estimated MN supply based on DOMR.
Kriopreservasi Semen Domba Garut dengan Pengencer Tris yang Disuplementasi Ethylene Diamine Tetraacetic Acid (CRYOPRESERVATION OF GARUT RAM SEMEN WITH TRIS EXTENDER ETHYLENEDIAMINETETRAACETIC ACID) Muhammad Rizal; Herdis .; Nasrullah .; Muhammad Riyadhi; Insun Sangadji; Yulnawati .
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ethylene diamine tetraacetic acid (EDTA) is a molecular structure that binds to heavy metals andother toxins which needed to protect the spermatozoa during the cryopreservation process. The objectiveof this research was to examine EDTA in maintain the quality of garut ram frozen semen. Semen werecollected from mature Garut ram by artificial vagina. Fresh semen were evaluated and divided in equalvolume into three tubes and diluted with Tris extender containing 20% egg yolk (TEY-20) as control, TEY-20 + 0.01% EDTA (EDTA0.01), and TEY-20 + 0.02% EDTA (EDTA0.02), respectively. Semen was loaded in 0.25 mL mini straw with the concentration of 100 million motile spermatozoa. Semen was equilibrated at5oC for three hours, then frozen and stored in liquid nitrogen container. Quality of spermatozoa includingpercentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) wereevaluated after diluting, equilibrating, and thawing. Data were analyzed using completely randomizeddesign with three treatments and five replicates. Means were compared significant difference test at 0.05significant level. Results of this research showed that mean volume, color, consistency, pH, mass movement,spermatozoa concentration, percentage of MS, percentage of LS, percentage of abnormal spermatozoa,and percentage of IPM of garut ram fresh semen were 0.87 mL, cream, thick, 6.98, 3, 4,296 million cell/mL,75%, 86.33%, 4.4%, and 85%, respectively. Percentages of MS, LS, and IPM after thawing in control (42.5,52.5, and 52.17%) was not significantly difference with EDTA0.01 (44.17, 52.5, and 52.5%) and EDTA0.02(39.17%, 51%, and 48.33%). In conclusion, the addition of EDTA in Tris extender can maintain quality ofGarut ram frozen semen, and its suitable using in artificial insemination program. Addition of EDTA cannot enhancing quality of garut ram frozen semen.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Protektivitas Sapi di Kabupaten Kupang Terhadap Penyakit Ngorok (Septicaemia Epizootica) (PROTECTIVITY AGAINST SEPTICAEMIA EPIZOOTICA OF COWS IN KUPANG DISTRICT) Hilda Susiyanti Debora Berek; Widagdo Sri Nugroho; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Controlling SepticemiaEpizooticae (SE) through vaccination program has been undertaken in KupangDistrict. However, numbers of fatal cases is still being reported. A cross sectional study have been undertakenin order to determine the prevalence and factors affecting herd immunity at farm level in Kupang District.A total of 434 cow’s sera from 87 farmers were collected and further analyzed using Enzyme-linkedImmunosorbent Assay (ELISA). An antibody titer of e”200 ELISA Unit was used as the indicator ofprotective immunity. The prevalence of herd immunity was 73.7% and vaccination coverage was 90.8%.Based on unweighted logistic regression analysis it was found that factors affecting the animals protectiveimmunity were: animal >2 years of age (r = +1.45601; OR = 4.3); housing system ( r = +1.03958; OR = 2.8);reservoir animals (r = +0.090147; OR = 2.5), the sex of the animal (female) (r = + 0.080138; OR =2.2); oncefrequency of vaccination (r = +0.61015; OR = 1.8); and 6-12 months post vaccination period (r = + 0.58968;OR = 1.8). Based on linier regression analysis, the prevalence of herd immunity was increased during rainyseason, whereas it was decreased when cows werebought from animal market or from other district.
Tingkah Laku Menetas Piyik Burung Weris (Gallirallus philipensis) dan Burung Dewasa dalam Penangkaran (HATCHING BEHAVIOR AND BEHAVIOR IN CAPTIVITY OF GALLIRALLUS PHILIPPENSIS) Lucia Johana Lambey; Ronny Rachman Noor; Wasmen Manalu; Dedy Duryadi
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study of behavior has a very important role in wildlife management process both for cultivationand for preservation in nature. The purpose of this study was to observe and document behaviors and dailyactivities of weris. Observation of hatching process and behavior of juvenile weris used direct observationmethod, while the behavior of adult weris in captivity used scanning sampling method. The data wereanalyzed descriptively. Weris had six stages of hatching processes starting from the breakdown of the eggshell until the chick moved out from the shell. The placement of the eggs in hatching machine was byputting the dull part on upside. Weris is a diurnal bird that active in the morning until late afternoon.Young and adult weris birds had different activities, i.e., adult birds did bathing and flying activities,while the young birds just rest and sleep. Observation on behavior of adult weris (n = 10) showed that thehighest activity for 12 hours was moving (314,6 minutes), then followed by resting (283,1 minutes), eating(51,8 minutes), drinking (29 minutes), bathing (22,9 minutes), and the lowest was preening (18,6 minutes).Inherited behavior pattern, such as bathing, did not change, eventhough the environment changed.
Pengimbuhan Ragi Roti dalam Pakan Meningkatkan Respons Imun Nonspesifik dan Pertumbuhan Ikan Nila (SUPPLEMENTATION OF BAKER’S YEAST IN FEED ENHANCE NONSPECIFIC IMMUNE RESPONSE AND GROWTH OF NILE TILAPIA) Henky Manoppo; Magdalena EF Kolopita
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to evaluate the efficacy of Baker’s yeast (Saccharomyces cereviciae) to enhancenonspecific immune response and growth of Nile tilapia fish (Oreochromis niloticus). The fish were obtainedfrom Freshwater Hatchery Tateli (BP3I), Marine and Fisheries Office, North Sulawesi. After acclimatizationfor two weeks in 1000 L fiberglass tank, fish with an average weight of 9 g were put in five 45 L-aquaria ata density of 15 fish/aquarium. During the experiment, fish were fed with feed pellet supplemented withfive different doses of baker’s yeast (10, 20, 30, 40 g yeast/kg pellet) for four consecutive weeks at 5% bw/day, twice a day. Fish in different aquarium received different dose of baker’s yeast. Immune parametersincluding total leucocyte count and phagocytosis activity and growth of fish were measured at two weeksinterval. After four weeks of feeding, total leucocyte count and phagocytosis activity of phagocyte cells offish fed pellet supplemented with 10 g yeast/kg pellet increased significantly as compared to that ofcontrol fish (p<0.01). Growth of fish fed pellet supplemented with 10 g yeast/kg pellet also increasesignificantly as compared to control group (p=0.01). Average weight gain of fish fed pellet supplementedwith 10 g yeast/kg pellet was 15.00±1.00 g while control fish was 8.33 g. As conclusion, supplementationof baker’s yeast in feed could enhance nonspecific immune response and growth of Nile tilapia fish.
Terapi Sel Punca Mesenkimal Sumsum Tulang Tikus dalam Meregenerasi Sel Sitotrofoblas Nekrosis yang Dipapar Carbon Black (RAT BONE MARROW MESENCHYMAL STEM CELL THERAPY IN REGENERATING NECROTIC CYTOTROPHOBLAST CELL FOLLOWING EXPOSED TO CARBON BLACK) Widjiati .; Sri Pantja Madyawati; Rimayanti .; Agung Budianto Achmad
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study is to find out the potency of Rat Bone Marrow Mesenchymal Stem Cell(RBMMSC) in regenerating necrotic cytotrophoblast cells of rats (Rattusnorvegicus) following exposure tocarbon black at day 6 of gestation at different time of exposure (6 days and 12 days). This study usedrandomized factorial design with two factors (gestation day and treatment). Forty-eight gravid femalerats were divided into six treatment groups i.e. (i) animals at day 6-11 gestation and not expose to carbonblack; (ii) 6-11 days gestation animals + 532mg/m3 carbon black for 4 hours; (iii) 6-11 days gestationanimals + 532mg/m3 carbon black for 4 hours +1x107/0.1ml RBMMSC intravenously; (iv) animals at day6-17 gestation and not expose to carbon black; (v) 6-17 days gestation animals + 532mg/m3 carbon blackfor 4 hours; (vi) 6-17 days gestation animals + 532mg/m3 carbon black for 4 hours +1x107/0.1ml RBMMSCintravenously, respectively. Data were analyzed using univariat analysis and analysis of variance. Theresults showed that there were no significance differences in regenerating necrotic cytotrophoblast betweenthe groups treated with RBMMSC and carbon black exposure. The results indicated that the stem celltherapy following exposure to carbon black was incapable in regenerating the necrotic cytotrophoblastcells.
Inventarisasi Cacing Parasitik pada Ikan Kembung di Perairan Teluk Banten dan Pelabuhan Ratu (THE HELMINTH PARASITES INVENTORY OF RASTRELLIGER SP. FROM BANTEN BAY AND PELABUHAN RATU BAY) Forcep Rio Indaryanto; Yusli Wardiatno; Risa Tiuria
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The monitoring of health and disease in a fish is important as parasitism plays a central role in fishbiology. Parasitism is a ubiquitous phenomenon in the marine environment and it is probable that allmarine fishes are infected with parasites. The aims of the research were to inventory the helminth parasitesof Rastrelliger sp. from Banten Bay and Pelabuhan Ratu Bay. As many as 25–30 of fish samples werecollected using gill net and examined for helminth parasites. The helminth parasitic calculated intensityand prevalence. The helminth parasites of Rastrelliger sp. were found Lechitocladium angustiovum (digenea:Hemiuridae), Lecitochirium sp. (digenea: Hemiuridae), Prodistomum orientalis (digenea: Lepocreadiidae)and Anisakis typica (nematodes: Anisakidae), with 90.12% of prevalence. L. angustonum was dominancehelminth parasites found in fish. There was no difference on parasites found in R. kanagurta and R.brachysoma wich were of Restrellinger genus. The location not appear have no significant after on helminthparasitic infection as they have a same genetic stock. Anisakis species in Java sea have a same genetipewith Anisakis typical and was not zoonotic parasite categories.
Kompetensi Perkembangan Oosit Kambing Kacang dengan Diameter Berbeda pada Medium yang Disuplementasi Cairan Folikel (DEVELOPMENTAL COMPETENCE OF KACANG GOAT OOCYTES WITH DIFFERENT DIAMETER ON MEDIUM WITH FOLLICULAR FLUID SUPPLEMENTATION) Ali Harris; Sri Rahayu; Gatot Ciptadi
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to determine the developmental competence of local goats oocytes on thebasis of their diameter in the medium supplemented with follicular fluid. Ovaries obtained fromSlaughterhouse, Malang City were collected and washed with NaCl containing streptomycin and penicillinat 32-35oC. Oocytes were obtained by follicular aspiration using a 10 ml syringe and needle 18 G needle.Oocytes were then divided into 3 groups on based at their diameter, group 1 (<160,5 ?m), group 2 (160,5-170,0?m) and group 3 (> 170,0?m). Follicular fluid was obtained by pooling the goat follicular fluid fromfollicles which have 3-8 mm in diameter. Maturation medium used was TCM-199 + 10% follicularfluid.Oocytes matured for 26 hours at 39oC and 5% CO2 with maximum humidity. Observation of oocytedevelopmental competence includes level cumulus cell expansion and maturation level of the nucleus. Theresults showed that the oocytes in groups 2 (160,5-170,0?m) and 3 (> 170,0?m) have a maximum ofcumulus expansion level 1 (63,8 and 74%) and metaphase II (56,6% and 68,5%).It is concluded that thediameter of oocytes had an influence on oocyte developmental competence (p <0,05), and can be consideredfor oocytes selection criteria.

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