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Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA" : 11 Documents clear
Efek pemberian ekstrak kedelai hitam (Glycine soja) terhadap histologi femur pada mencit (Mus musculus) ovariektomi mahhriani mahriani; Resa Miftahatu Yuniar; Masrurotul Hasanah; Eva Tyas Utami
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p05

Abstract

Bone is a dynamic structure and has the process to remodelling or regeneration. Bone remodelling includes the process of formation by osteoblasts and the process of resorption by osteoclasts. The balancing between formation and resorption are assisted by the estrogen hormone.Estrogen deficiency can lead the bone resorption. Ovariectomy is a model for estrogen deficiency by removing the ovaries. One way to overcome estrogen deficiency can be solved by offering phytoestrogens. This research have purpose to determine the effect of black soybean extract (Glycine soja) on the histology of the femur mice (Mus musculus) ovariectomized. In this research used a dose of black soybean extract as many as 0.42 g /ml/day and 0.63 g/ml/day, which are given orally for 30 days. The research is conducted with a completele randomized design with 4 treatments and 6 replications. Observations are made on the number of osteoblasts and the number of osteoclasts on the histological of the femur. The results showed that offering black soybean extract at a dose of 0.42 g/ml/day for 30 days have significantly increased the number of osteoblasts and have decreased the number of osteoclasts in the femur of ovariectomized mice.
Pengelolaan sampah anorganik pada Alila Villas Uluwatu, Bali Sulfa Ilmiyah; A.A.G. Raka Dalem; I Ketut Muksin
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p09

Abstract

Environmental problems are complex things that they are very influential on all components in the ecosystem. One of the environmental problems is caused by the production of waste that is not treated properly, for example in the hotel industry. Tourism is one of the activities that produce various kinds of waste, both environmentally friendly and not. Products that are not environmentally friendly can pollute the environment if they do not handled properly. Alila Villas Uluwatu (AVU) is one of hotels in Bali that are committed to environmentally friendly and efforts to protect the environment by minimizing waste production. The objective of this research was to find out AVU's strategies in protecting the environment, especially in handling the resulting inorganic waste, such as glass, paper and plasctics. The method utilized in data collection on this study was through conducting interview, direct observation in the field and examination of related documents. Data analysis was performed descriptively, either in qualitative and quantitative forms. The results showed that the waste management strategy in AVU included efforts to minimize inorganic waste production and by using more environmentally friendly materials such as toothbrushes with wooden handles, paper straws and bamboo straws.
Penapisan toleransi kekeringan padi lokal Lampung pada fase perkecambahan Lili Chrisnawati; Yulianty Yulianty; Eti Ernawiati; Umilia Fitriyani; Aprilia Eka Putri
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p01

Abstract

Seed germination is one of the most critical stages in plant growth. Screening at this stage can provide preliminary information on drought tolerance. This study aims to evaluate drought tolerance in local Lampung rice varieties Lumbung Sewu Cantik in the germination phase. This study used a completely randomized design with three concentrations of PEG and three replications. The PEG concentrations given were 0%, 10%, and 15%. The observed data were analyzed for variance at the level a test = 0.05 and further analysis used Duncan's Multiple Range Test (DMRT). The results showed that the higher the PEG concentration, the plumulae length and seminal root length of rice decreased. However, in radicles, PEG 20% did not decrease length growth. Giving PEG 20% also did not decrease the dry weight of the radicles and in the seminal roots, there was no significant difference in weight between PEG 10% and PEG 20%. This indicated that Lumbung Sewu Cantik Padi has the potential to tolerate drought in the germination phase.
DNA barcoding pada invertebrata laut Agus Mohammad Hikam; Nurul Jadid Mubarakati; Muhammad Dailami; Abdul Hamid A. Toha
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p06

Abstract

A large number of species of marine invertebrates that have similar morphology cause misidentification very possible. Molecular species identification is important in taxonomy study accurately. This study aims to determine the identity of Aquatic Papua marine invertebrates with DNA barcoding technique using markers COI gene (Cytochrome c oxidase I). The study identified 29 individuals of marine invertebrates, which came from sea cucumbers (6 samples), lobsters (6 samples), octopus (6 samples), chitons (5 samples), and sea urchins (6 samples). Molecular identification methods consist of DNA isolation, PCR COI gene fragments, sequencing, and DNA sequence analysis using bioinformatics software. The results showed that the sea cucumber came from the Bohadschia marmorata species, the lobster came from the Panulirus versicolor species, the octopus came from the Octopus cyanea species, the chiton came from the Ischnochitonaustralis species, and the sea urchins came from the Tripneustesgratilla species. The similarity of samples and references are in the range of 84.58 to 100.00%. The disparity index, genetic distance, and phylogenetic tree supported this result.
Histomorfometrik duodenum kelinci jantan setelah diberi pakan komersial disuplementasi minyak hati ikan kod Ni Gusti Ayu Manik Ermayanti; Ni Wayan Sudatri; Ni Nyoman Wirasiti; I Gusti Ayu Manik Widhyastini
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p10

Abstract

Fish oil is a source of long-chain polyunsaturated fatty acids (LCPUFA) that can be used as supplementary feed in commercial feed to increase male rabbit productivity. PUFAs function in various physiological processes, including in the digestive system.This study aimed to determine the histomorphometrics of male rabbits duodenum after fed commercial feed supplemented by cod liver oil. The experimental design used was a Completely Randomized Design (CRD) with four feed packages, namely commercial feed without cod liver oil (P0) as a control, commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2) and 6% (P3). Each consists of five male local rabbits as replicates and given starting female rabbits 1-3 months. The parameters observed were villi height, villi width, crypt depth, and tunica mucosa thickness of the duodenum in male rabbits. The data obtained were analyzed with Anova and if significantly different were further tested with DMRT. The results showed that cod liver oil supplementation in commercial feed was significantly different (p<0.05) to the histomorphometric parameters (villi height, villi width, crypt depth, and thickness of the tunica mucosa) of the duodenum in male rabbits. Can be concluted that supplementation of cod liver oil in commercial feed can increase the histomorphometrics of male local rabbits duodenum.
Karakter morfologis dan molekuler udang regang (Macrobrachium sintangense De Man, 1898) dari Daerah Istimewa Yogyakarta Rini Rahmawati; Rury Eprilurahman; Lukman Hakim; Tuty Arisuryanti; Zuliyati Rohmah; Trijoko Trijoko
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p02

Abstract

Macrobrachium genus has many species members and distributed in various countries including Indonesia. One of the species belonging to the Macrobarachium genus that can be found in Indonesia was Macrobrachium sintangense or known as Sunda River Prawn. Identification of this species is important as an effort to expand studies on Sunda River Prawn in the Region of Yogyakarta. Identification of this species was carried out morphologically and molecularly with the 16S gene. The purpose of this study was to determine the morphological and molecular characters of M. sintangense. Morphological identification is done by identifying morphological, morphometric, and meristic characters according to the identification key. Molecular analysis was carried out by isolation, amplification, electrophoresis, and DNA visualization with UV illuminators, DNA sequencing by 1st Base. The results of this study indicate the morphological character of the rostrum slightly curved upwards or like a knife, rounded post antennular carapace, absent preanal carina, pubescene in the proximal half to two thirds of the chela. Morphologically, all samples were identified as members of the genus Macrobrachium. There are eight (8) specimens identifiable to the species level, as M. sintangense. The results of molecular identification of two (2) specimens showed a high similarity value and a close relationship with M. sintangense from GenBank samples and also close relationship with M. sintangense from Tukad Panti, Bali, Indonesia. The conclusion of this research is that morphologically and molecularly, the samples found in the Winongo.
Karakter morfologis dan molekular udang air tawar anggota Macrobrachium pilimanus species group di Sungai Opak, Winongo dan Sempor, Daerah Istimewa Yogyakarta Malya Adzillina Silmi; Rury Eprilurahman; Trijoko Trijoko; Lukman Hakim
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p07

Abstract

Macrobrachium is a genus of the Crustacea subphylum with the highest diversity. Identification of the morphology of the genus Macrobrachium is mostly challenging due to the characters for species level identification are complicated, whereas many characters are common to all Macrobrachium species. In addition, it is difficult to identify morphology because of the influence of social dominance in males. Therefore, another approach is needed to identify Macrobrachium accurately, one of which is by barcoding DNA using the 16S mitochondrial gene. This study was aimed to determine the morphological and molecular characters of Macrobrachium pilimanus species group based on the 16S mitochondrial gene from the Opak, Winongo, and Sempor rivers, in Daerah Istimewa Yogyakarta. Furthermore, shrimp samples were identified based on morphological, meristic, and morphometric characters. Meanwhile, molecular analysis uses 16Sar as forward primer and 16Sbr as reverse primer. Based on the morphological identification results, the shrimp samples obtained could only be identified up to the level of the Macrobrachium genus. In addition, the shrimp samples can also be included in the Macrobrachium pilimanus species group based on the general characteristics of the species group. The shrimp samples in this study have highest similarities in character with the species M. pilimanus and M. empulipke distributed in Java. Based on molecular analysis, sample from Winongo River (WNM1) has a similarity of 87.63% with Macrobrachium forcipatum (JQ362454.1) and 87,02% with M. dienbienphuense (JQ390474.1), which means that it is a different species from the species in the GenBank. Macrobrachium forcipatum and M. dienbienphuense are members of M. pilimanus species group. The conclusion of this research is that all the samples are member of M. pilimanus species group.
Meta-Analisis: Efektifitas pemberian pakan buatan untuk meningkatkan laju pertumbuhan dan kualitas reproduksi spesies Monopterus albus Tomi Apra Santosa; Rani Dwi Suci Hd Putri; Ramadhan Sumarmin; Dwi Hilda Putri; Abdul Razak
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p11

Abstract

The use of artificial feed for fish farming has been widely used at this time, however, it is not yet known the significant effect of artificial feed on the growth and reproduction rate of Monopterus albus. This study aims to determine the effectiveness of artificial feeding on the growth and reproduction rate of Monopterus albus species. This research is meta-analysis research. The data source comes from a search of 18 national and international articles published in 2010-2020 related to feeding the Monopterus albus species. Data obtained from google scholar database, DOAJ, ScienceDirect, sagejournal, Springer, and IEEE. The data analysis technique is a qualitative descriptive analysis with JASP software. The results showed that 35% of artificial feeding affected the growth of Monopterus albus and 28.5% affected the reproductive rate with an effect size of 1.2. This shows that artificial feeding has an effect of 0.334 in increasing the growth and reproductive quality of Monopterus albus.
Variasi genetik dan jarak genetik suku Sakai di Provinsi Riau dengan suku Minangkabau di Desa Pagaruyung Sumatera Barat berdasarkan DNA mikrosatelit Tyara Tyara; I Ketut Junitha; Ni Nyoman Wirasiti
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p03

Abstract

There are several versions can explain the origin of the Sakai tribe. Sakai people themselves believe that their ancestors came from Pagaruyung, West Sumatra Province. The purpose of this research is to determine the genetic variation and genetic distance of the Sakai tribe in Riau Province and the Minangkabau tribe in Pagaruyung Village, West Sumatra Province based on three microsatellite DNA loci (D2S1338, D16S539, and D13S317). A total of 10 alleles were found in three microsatellite loci of the Sakai tribe and 11 alleles in the Minangkabau tribe. Data analysis using GenAlex 6.503 software showed that the heterozygous value of the Sakai and Minangkabau tribes was moderate with a value of 0.557 ± 0.062 in the Sakai tribe and 0.615 ± 0.073 in the Minangkabau tribe. Meanwhile, the value of genetic differentiation (F’ST) in these two tribes shows moderate genetic differences (0.088 ± 0.039). Genetically, Sakai people and Minangkabau tribes have similarities based on the D2S1338, D16S539, and D13S317 microsatellite DNA loci with a genetic distance of 0.269 and the proportion of identical genes (Genetic identity) of 0.764. This result is supported by alleles outflow or entry in the Sakai and Minangkabau tribes with a migration rate is four people per generation.
Pertumbuhan planlet anggrek Cymbidium bicolor Lindl. pada tahap subkultur dengan variasi media Fakhri Fadhlurrohman Pratama; Nintya Setiari; Yulita Nurchayati
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p08

Abstract

Cymbidium bicolor is one of the orchids in Indonesia which has beautiful and longlasting flowers, so it has potential to become an ornamental plant and has economic value. The method of propagation generally is tissue culture. One of the stages in plant tissue culture is subculture. The variation of media used at the subculture stage make an effect for growth of plantlet. Media usually can be used include Murashige and Skoog (MS), Vacin and Went (VW), and New Phalaenopsis (NP). This study aims is to analyze the variation of media during the subculture. The method used was tissue culture in subculture with media as treatment. Cymbidium bicolor planlets were subcultured into MS, VW, and NP and observed for 2 months. The parameters observed were the leaf emergence, the number of leafs, leaf length, leaf width, the number of roots, root length, and the number of shoots. This study used a completely randomized design with single factor and 15 replicates. Data were analyzed by ANOVA with 95% significance. Treatment of variation of media significantly affected all parameters. Murashige and Skoog (MS) medium was the best medium for leaf emergence (11 days), the number of leafs (4,92), leaf length (1,56 cm), leaf width (0,33 cm), the number of roots (2,67), root length (2,73 cm), and the number of shoots (4,00). Murashige and Skoog (MS) medium was the best medium and concentrations for C. bicolor growth at the subculture stage.

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