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MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.) Nadya Febri Harlifia; Bambang Irawan; Salman Farisi; Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.163

Abstract

Bamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.)
BROMELAIN WASTE TEA COMPOST INDUCED BY LIGNINOLYTIC INOCULUM OF Trichoderma sp. ON THE GROWTH OF LEAF NUMBER AND CHLOROPHYLL CONTENT OF CHILI (Capsicum annuum L.) Syafira Clarisa Huda; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.165

Abstract

Pineapple is one kind of fruit that contains a lot of nutrition.  The waste from residual pineapple processing is called bromelain.  Bromelain contains polymer complex in the form of organic sources from nature if it could be decomposed properly.  One way to exploited bromelain is by composting.  Composting is done with the help of fungi of Trichoderma sp. to speed up the decomposition process.  The resulting compost is aerated to increase the nutrients and form a further decomposition process in the soil.  The research was carried out from January till March 2021 in the Microbiology Laboratory Biology, Faculty of Mathematics and Science, University of Lampung.  The parameters of this research are the content of chlorophyll and the number of leaves.  This research used a completely Randomized Design with 7 treatments that are A1 (24 hours of mixed bromelain), A2 (48 hours of mixed bromelain), A3 (72 hours of mixed bromelain), A4 (24 hours of pure bromelain), A5 (48 hours of pure bromelain), A6 (72 hours of pure bromelain), and A7 (control).  Based on this research the value of the highest chlorophyll A on A6 treatment is 0,655.  A value of the highest on chlorophyll B on A6 treatment that 0,429.  The highest total chlorophyll on the A6 treatment is 1,048.  Based on this research the A4 treatment has the highest average number of leaves compared to other treatments.    
APPLICATION OF BROMELAIN LITTER SOLID COMPOST INDUCED BY LIGNINOLITIK Trichoderma sp. FUNGUS TOWARDS NUMBER OF LEAVES AND CHLOROPHYL CONTENT CHILI PLANTS (Capsicum annuum L.) Jihan Haura; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.166

Abstract

Piles of uncontrolled pineapple peel waste have a negative impact that will affect various aspects of life, either directly or indirectly. Therefore, pineapple peel waste that can no longer be processed can be used for making compost. The taste of pineapple can be degraded biologically with the help of enzymes that can be produced by microorganisms. One of the microorganisms that can support the decomposition process is a group of fungi. In this study, the fungus Trichoderma sp. (Bioggp 5) is a ligninolytic fungus. Trichoderma sp. is a saprophytic soil microorganism that attacks natural pathogenic fungi and is beneficial to plants. The purpose of this study was to determine the effect of solid bromelain compost inoculum of ligninolytic fungi (Trichoderma sp.) on leaf number and chlorophyll content of chili plants (Capsicum annum L.) and the best dose of compost that could be used. This study used a completely randomized design (CRD) with 7 treatments, namely T0 (control), T1 (1.4% pure bromelain), T2 (1.7% pure bromelain), T3 (2% pure bromelain), T4 (1, 4% bromelain + leaf litter), T5 (1.7% bromelain + leaf litter), T6 (2% bromelain + leaf litter). The parameters used were the number of leaves and chlorophyll content. And the results obtained from this study were the T3 dose (2% pure bromelain) was the best dose for the number of leaves, while the T2 dose (1.7% pure bromelain) was the best dose for chlorophyll content.
DINAMIKA POPULASI BAKTERI DAN TOTAL ASAM PADA FERMENTASI BEKASAM IKAN PATIN (Pangasius hypopthalmus) Bella Noor Arfianty; Salman Farisi; Christina Nugroho Ekowati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 4 No. 2 (2017)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v4i2.133

Abstract

Bekasam merupakan produk makanan tradisional hasil fermentasi dari ikan air tawar dengan penambahan nasi dan garam. Selama proses fermentasi, sumber karbohidrat dipecah menjadi gula-gula sederhana, kemudian diubah menjadi alkohol dan asam. Penggunaan jenis ikan pada pembuatan bekasam berpengaruh terhadap jumlah bakteri asam laktat dan kadar asam laktat yang dihasilkan. Penelitian ini bertujuan untuk mengetahui jumlah total bakteri dan bakteri asam laktat serta kadar asam laktat yang dihasilkan selama proses fermentasi bekasam ikan patin siam (Pangasius hypopthalmus). Penelitian ini dilaksanakan pada bulan Januari sampai Februari 2017 di Laboratorium Mikrobiologi, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung. Penelitian ini menggunakan metode titrimetri untuk menentukan kadar asam laktat dan metode Angka Lempeng Total untuk menentukan jumlah bakteri. Pengamatan dilakukan setiap 2 hari, 4 hari, 6 hari, 7 hari, 8 hari dan 10 hari dengan 2 kali ulangan. Parameter yang diamati meliputi: penghitungan jumlah total bakteri dan bakteri asam laktat , pengukuran kadar asam laktat, pengukuran pH serta uji organoleptik. Hasil penelitian menunjukkan bahwa nilai total bakteri asam laktat meningkat sampai fermentasi hari ke-6, nilai total bakteri mengalami penurunan, total asam tertitrasi meningkat, sedangkan nilai pH menurun.
ISOLATION OF Salmonella typhoid 16s rRNA GENE FRAGMENT BASED ON POLYMERASE CHAIN REACTION (PCR) Salman Farisi; Wawan Abdullah Setiawan; Suratman Umar
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 2 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i2.157

Abstract

Iridescent is one of the traditional fish fermentation products that has a sour taste and is widely known in various regions of Indonesia. In its metabolism LAB produces organic acids, hydrogen peroxide, diacetyl, CO2 and bacteriocin. These compounds are antimicrobial compounds that inhibit the growth of pathogenic bacteria and bacterial decomposition. The purpose of this study was to determine the antibacterial activity produced by lactic acid bacteria isolates from catfish (Pangasius hypophthalmus) inhibiting the growth of Escherecia coli. From iridescent obtained 9 isolates that have different characteristics from each other by the morphology of LAB isolate colonies which include colony color, colony shape, colony elevation and colony margin. After production of antibacterial, all isolates showed positive result when antibacterial tested againts Escherecia coli either in acidic condition or neutralized.
MORTALITAS DAN RESORPSI FETUS MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK ETANOL TANAMAN SURUHAN (Peperomia pellucida (L.,) Kunth.) Hendri Busman; Nuning Nurcahyani; Yosi Dwi Saputra; Salman Farisi; Qotrunnada Salsabila
Jurnal Kesehatan Kusuma Husada Vol. 12 No. 2, Juli 2021
Publisher : Universitas Kusuma Husada Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.457 KB) | DOI: 10.34035/jk.v12i2.715

Abstract

Tanaman Suruhan (Peperomia pellucida (L.,) Kunth.) merupakan tanaman obat yang memiliki senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, dan steroid. Berbagai senyawa kimia tersebut yang berpotensi sebagai obat tetapi perlu diperhatikan kemungkinan adanya efek samping terhadap organisme khususnya pada masa kehamilan. Penelitian ini bertujuan menguji efek teratogenik ekstrak tanaman suruhan terhadap fetus mencit (Mus musculus L.), meliputi mortalitas dan resorpsi. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan 20 ekor mencit betina dibagi ke dalam 4 kelompok yaitu: K(+) (Aquabides), P1 diberi ekstrak tanaman suruhan dengan dosis (1,68 mg/g bb), P2 (3,36 mg/g bb), dan P3 (6,72 mg/g bb). Hasil penelitian terhadap persentase fetus yang mengalami mortalitas dan resorpsi fetus antara kontrol K(+) dan perlakuan dengan ekstrak etanol suruhan (P1, P2, dan P3) tidak menunjukkan perbedaan yang bermakna secara statistik berdasarkan uji ANOVA satu faktor (p value 0,418). Hasil penelitian yang didapat menunjukkan bahwa ekstrak etanol tanaman suruhan tidak menyebabkan mortalitas pada fetus mencit, namun menyebabkan resorpsi pada fetus mencit pada pemberian dosis sebesar 1,62 mg/g BB, 3,36 mg/g BB, dan 6,72 mg/g BB. Pepper elder (Peperomia pellucida (L.,) Kunth.) is a medicinal plant that has secondary metabolites such as alkaloids, flavonoids, saponins, tannins, and steroids. The side effects for organisms of those chemical compounds, which are potentially beneficial for their medicinal use, still need to be considered especially in pregnancy. This study aims to determine the teratogenic effects of pepper elder extract on mortality and resorption of mice (Mus musculus L.) fetus. This study uses total random sampling design with 4 treatments consisting of 20 female mice divided into 4 groups: the control group (K(+)) which is given Aqua distillation and the treatment groups which are all given the pepper elder extract with different doses, where the first treatment group (P1) is given 1.68 mg/g body weight of the extract, the second treatment group (P2) is given 3.36 mg/g body weight of the extract, and the third treatment group (P3) is given 6.72 mg/g body weight of the extract. The results obtained of mortality and resorption percentage of mice fetus between the control group (K(+)) and the treatment groups (P1, P2, and P3) showed that there is no statistically meaningful difference based on one-way ANOVA test (p value 0,418). This study showed that ethanol extract of pepper elder causes no mortality in mice fetus, yet it causes resorption on mice fetus at given doses of 1,62 mg/g body weight, 3,36 mg/g body weight, dan 6,72 mg/g body weight.
Pemberian Kompos Bahan Sampah Serat Bromelain Yang Diinduksi Inokulum Fungi Selulolitik Aspergillus sp. Pada Pertumbuhan Vegetatif Cabai (Capsicum annuum L.) Fadila Raisyadikara; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Biologi Papua Vol 14, No 1 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.287 KB) | DOI: 10.31957/jbp.1701

Abstract

 The purpose of this research is to understand the effect and the best dossage of bromelain wastes solid compost for vegetative growth of chilli plants. Accumulation of pineapple wastes made by the large pineapple plantation area. The litter of pineapple bromelain enzyme (bromelain wastes) still containing organic matter that could composted for plant nutrition. The composting of it can be accelerated by induction inoculum of cellulotic Aspergillus sp. fungus. The research used Completely Randomized Design method with seven treatment and three replications. The treatment used two different type of compost. The composition of compost A type is:  0,5 kg bromelain + 0,5 kg leaves litter + 0,5 kg livestock wastes + inoculum 1%. The composition of compost B type is:  1 kg bromelain + 0,5 kg livestock wastes + inoculum 1%. The treatment conducted P0:  5 kg soil (control). P1:  5 kg soil + 1,4% A type compost. P2: 5 kg soil + 1,7% A type compost. P3: 5 kg soil + 2% A type compost. P4: 5 kg soil + 1,4% B type compost. P5: 5 kg soil + 1,7 % B type compost. P6: 5 kg soil + 2% B type compost. Variance analyzed of obtained data were using ANOVA and tested using the Least Significant Difference Test (LSD) method. Parameter of this study are number of leaves and chlorophyll content. The results showed that the application of P6: 2% dossage B type compost from bromelain solid compost could optimized the vegetative growth of chili plants (Capsicum annuum L.). Key words: Aspergillus sp.; bromelain; chili; compost.
Uji Tantang Bakteri Bacillus Kandidat Probiotik secara Invitro terhadap Bakteri Vibrio harveyi Sumardi Sumardi; Salman Farisi; Christina Nugroho Ekowati; Rizka Oktavia
Jurnal Biologi Papua Vol 11, No 2 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.657 KB) | DOI: 10.31957/jbp.799

Abstract

This study aims to isolate Bacillus that can fight the growth of Vibrio harveyi . Based on the results of the inter-Bacillus competition test show that Bacillus isolates was able to compete and grow with each other on the SWCA media. The challenge test Bacillus bacterial to against Vibrio harveyi bacteria, that Bacillus did not yet produce anti-bacteria on the second day. In the joint culture test method between Bacillus and Vibrio harveyi that Bacillus were able to inhibit the growth of Vibrio harveyi bacteria on the 4th day.
Effect of Pyriproxyfen, a Malaria Vector Larvicide, on the Growth and Mortality of Vannamei Shrimp Sriana Putri; Endah Setyaningrum; Salman Farisi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.258

Abstract

Anopheles mosquito shared a breeding site with the vannamei shrimp (Litopenaeus vannamei). Anopheles mosquito larvae commonly live in both stagnant fresh and brackish water. The potential breeding habitat of Anopheles mosquito larvae may be rice fields, pools or ponds, swamps, ditches, and slow-current streams. One of the effective strategies to control malaria is to apply chemical insecticides. Pyriproxifen is a larvicide that contains juvenile hormone-like compounds. Juvenile hormone-like compounds can affect mosquito morphogenesis, characterized by the failure of the larvae to develop into pupae. With a completely randomized design, the shrimp fry was divided into 4 groups,  6 repetitions, 20 individuals each. Shrimp fry in each experimental unit was placed and maintained in plastic jars containing 2.5 L of brackish water. The results showed that the insect growth regulator (IGR) with the active ingredient pyriproxyfen at various concentrations had no significant effect on the growth, mortality, and survival of vannamei shrimp fry.  It can be concluded therefore that pyriproxifen larvicide was safe to be used for controlling malaria mosquito vectors in hatcheries and breeding ponds of vannamei shrimp.
Pelatihan Kewirausahaan Pembuatan Kompos Unggul Dari Seresah Daun Dengan Induksi Inokulum Fungi Pada Mahasiswa Program Studi Bahasa Arab Sekolah Tinggi Ilmu Tarbiyah Darul Fattah Bandar Lampung Salman Farisi; Bambang Irawan; Suratman; Sutyarso; Hendri Busman
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 8 : September (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Compost is the final substance in the fermentation process of piles of waste/plant litter including animal carcasses. The fermentation needs to be accelerated with human assistance. This saprotrophic fungi isolate has the potential to be developed as a compost inoculum in the manufacture of organic fertilizers. Compost induced with fungal inoculum is able to produce organic fertilizer relatively quickly and contains high plant nutrients. The prospect of the need for organic fertilizers is quite high in supporting organic agriculture, so it is necessary to disseminate information on science and technology and training to the public on the manufacture of organic fertilizers by induction of saprotrophic fungi inoculum. In addition, the availability of garden plant litter is abundant and free from chemical residues. The results of this activity showed that there was an increase in the knowledge and understanding of the training participants in making compost from leaf litter. The average increase in the knowledge of the trainees is 25,25 %.