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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan BALI MEDICAL JOURNAL (BMJ) Journal Pharmaceutical Science and Application (JPSA) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Farmasi Udayana Journal of Tropical Pharmacy and Chemistry JURNAL ILMU KEFARMASIAN INDONESIA Jurnal Ilmiah Medicamento Prosiding Workshop dan Seminar Nasional Farmasi (WSNF)
Ni Putu Ariantari
Study Program Of Pharmacist Profession, Faculty Of Mathematics And Natural Sciences, Udayana University, Jimbaran, Badung, Bali 80361
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : Farmasains : Jurnal Farmasi dan Ilmu Kesehatan

 1 
EFEK SITOTOKSIK PROTEIN DARI DAUN Mirabilis jalapa l HASIL PEMURNIAN DENGAN KOLOM CM SEPHAROSE CL-6B TERHADAP KULTUR SEL HeLa Ni Putu Ariantari, Zullies Ikawati, Sudjadi, Sismindari
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 1 No. 1 (2010): April-September 2010
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/far.v1i1.419

Abstract

Mirabilis jalapa l leaves have been known containing Ribosome Inactivating Proteins (RIPs) base on its N-glycosidase activity and cleavage of supercoiled double stranded DNA. The research aims to investigate cytotoxic activity of protein isolated from Mirabilis jalapa l leaves against HeLa cell culture. In this research, crude extract of Mirabilis jalapa l leaves was separated by gradual precipitation using sulphate ammonium followed by dyalisis. Further separation of protein fraction with cation exchange chromatography using CM sepharose CL-6B column and elution with sodium phosphate buffer 5 mM pH 6,5 for 30 minutes continued with sodium phosphate buffer 5 mM pH 6,5 containing 0-0,5 M NaCl by gradient elution for 90 minutes. Cytotoxicity activity testing of protein fraction that showed an ability to cleave supercoiled double stranded DNA revealed that RIPs eluated at NaCl gradient of 0,33-0,37 M and 0,38-0,41 M, concentration of 0,553 mg/mL relatively less toxic against HeLa cell culture with percentage of cytotoxicity of 29,53%. Keywords: Mirabilis jalapa l, RIPs, cytotoxicity
Co-Authors 1Dwija, I.B.N.P Achmad Fuad Hafid Adiluhur, I.M.P. Adiluhur, M. A. Agastia, I.P.A. Agastia, P. A. A. Anggreni, M Astuti, K.W. Astuti, M.A.P. Aty Widyawaruyanti Caresya Insani Bangga Nabila Dewa Ayu Swastini Dewi, I. A. M. Dewi, I.A.M.K. Dwicandra, N.M.O. Dwija, I. B. N. P. Dwija, I.B.N.P Elizabeth S. P. Ratnasantasyacitta I Ketut Juniarta I Made Kardena I Putu Yogi Astara Putra I Putu Yogi Astara Putra I Wayan Sudiarta I. Setyawati I.B.N.P. Dwija I.G.N.A.P. Putra Ida Bagus Nyoman Putra Dwija Karso, F. P. Ketut Widyani Astuti LIDYA TUMEWU Mahadewi, S. A. Mahadewi, S.A. Mita Anggraeni, Mita N. W. Erawati Nallakrishna, I P. A. Ni Kadek Ari Kristiani Ni Luh Putu Indah Suryani Ni Luh Rustini Ni Made Suartini Ni Putu Eka Leliqia Ni Wayan Prasanthi Swarna Putri Ni Wayan Prasanthi Swarna Putri Nonye Treasure Ujam Nonye Treasure Ujam Normansyah, A. P. A. C. Kusuma P. A. C. Kusuma, P. A. C. Partiwisari, N.P.E. Priskila Putri Mairing Purwani, S. T. D Purwani, S. T. D. Putra, I. G. N. R. Putu Aurora Vanadis Putu Oka Samirana Ramayati, N.P.A Rasmaya Niruri Rini Noviyani S. T. D Purwani, Sagun Chandra Yowani Samiantara, I. K. Sang Ayu Made Dwi Ariesta Putri Savitri, L.P.V.A Yanti, P. E. W.