Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

4.919

P-Index

This Author published in this journals

All Journal Medicina INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES E-Jurnal Medika Udayana Indonesian Journal of Cancer Jurnal Ilmiah Kedokteran Wijaya Kusuma ISM (Intisari Sains Medis) : Jurnal Kedokteran Majalah Patologi Indonesia Indonesian Journal of Cancer Journal of Global Pharma Technology

I Gusti Ayu Sri Mahendra Dewi

Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia

Author-ID : 2392701

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 49 Documents Claim Missing Document

Claim Missing Document

Articles

Title

1 2

Clinicopathological patterns of cervical carcinoma in pathological anatomy laboratory at RSUP Sanglah Denpasar, Bali since 2012-2016 Shameni Subramaniam; I Wayan Juli Sumadi; I.G.A. Sri Mahendra Dewi
Intisari Sains Medis Vol. 11 No. 1 (2020): (Available online: 1 April 2020)
Publisher : DiscoverSys Inc.

Background: Carcinoma of the cervix is the second most common cancer in women worldwide, Cervical cancer is the most common cancer in women in developing countries. Cervical cancer is the fourth most common cancer in women overall, with an estimated 528,000 new cases in 2012. According to the data clinicopathology of cervical cancer in Bali its still in a small amount.Aims: To obtain information about clinicopathology of cervical carcinoma from the year 2012 â€“ 2016.Methods: A descriptive design study is conducted to determine the clinical pathology profile of cervical carcinoma among patients in Sanglah Hospital, Denpasar, Bali in the year 2012 â€“ 2016. Medical records of the patients were used as the secondary data for this research. This research used the histopathologic document that available in Pathological Anatomy at RSUP Sanglah which mainly reports about patient condition starting from the early anamnesis period until the exact diagnosis of the patient which is due to the laboratory examination or other.Result and Conclusion: As of 2012 to 2016, there were 142 cases of cervical cancer patients in RSUP Sanglah.The age group of 41-50 years has the highest number of patients in the year 2012 â€“ 2016 which is about 52 patients. Abnormal vaginal bleeding is the most common clinical finding of cervical cancer patients from the year 2012- 2016 and carries 53% in 142 cases. It is found out that the most common histopathologic type of cervical cancer from the year 2012 â€“ 2016 is squamous cell followed by adenocarcinoma in the list.

Entitas histopatologi penderita perdarahan uterus terkait gangguan hormonal di Laboratorium Patologi Anatomi Rsup Sanglah tahun 2014 -2018 Ida Ayu Eugenia Natasha Blanco Oka; IGA Sri Mahendra Dewi; Herman Saputra; Indraguna Pinatih; Indraguna Pinatih
Intisari Sains Medis Vol. 10 No. 2 (2019): (Available online: 1 August 2019)
Publisher : DiscoverSys Inc.

Introduction: Dysfunctional uterine bleeding (DUB) is a major cause of a significant burden of health care for women, their families, and the community as a whole, leading to high morbidity for women who experience it. The purpose of this study is to determine the prevalence, characteristics of patients, and histopathological description in patients with DUB. Method: This study uses a secondary data analysis method in the medical record data of patients with DUB who has been histopathologically examined at the Anatomy Pathology Laboratory, Sanglah General Hospital Denpasar in the period 2014 to 2018.Result: From the period 2014 to 2018, 24 cases of DUB were found, the highest number was found in the pre menopause age (41-50 years) in 15 cases (62.5%), based on the type of estrogen related bleeding hormonal disorders in 17 cases (70.8%), and 7 cases of progesterone related bleeding (29.2%). The most histopathological type in the estrogen related bleeding group was 13 disordered proliferative phases (54.2%). In the group with progesterone related bleeding, the highest histopathological picture was irregular shedding in 5 cases (20.8%).Conclusion: Estrogen related bleeding pattern still dominating the histopatological pattern in the dysfunctional uterine bleeding at Pathology Anatomy Laboratory, Sanglah General Hospital period 2014-2018.

Karakteristik gambaran sitologi pap-smear sebagai tes skrining untuk lesi pra-kanker serviks di RSUP Sanglah, Denpasar, Bali Putu Risky Yoga Pradnyana; Anak Agung Ayu Ngurah Susraini; I Gusti Ayu Sri Mahendra Dewi
Intisari Sains Medis Vol. 10 No. 3 (2019): (Available online: 1 December 2019)
Publisher : DiscoverSys Inc.

Background: Cervical cancer is the second most often cancer that occurs in women worldwide after breast cancer and as many as 84% of the cases occur in developing countries. Pap Smear is a screening method that widely used for cervical cancer screening. The aim of the study is to obtain the characteristic data of pap smear cytology as a pre-cervical cancer screening in Sanglah General Hospital 2016 â€“ 2017 period.Methods: This study was descriptive research with cross sectional study design. The sampling technique is total sampling where the research data comes from the cytology registration book results of the Anatomical Pathology Laboratory at Sanglah General Hospital Denpasar 1 January 2016 - 31 December 2017 period which has fulfilled the inclusion criteria and does not meet the exclusion criteria. Data were analyzed using SPSS version 20 for Windows.Results: This study collected as many as 590 samples. Most of the pap smear patients at Sanglah General Hospital Denpasar were 41-50 year old group as many as 188 people (31.86%), most of the patients parities were nullipara parities as many as 324 people (54.92%), most had no complaints as many as 261 people (44.24%) and most of the pap smear cytology description was Reactive Cellular Changes Associated with Inflammation (RCCI) as many as 261 people (44.64%).Conclusion: Most of the respondents who underwent pap-smear test as a screening step for pre-cancerous lesions had a cytological picture of Reactive cellular changes associated with Inflammation (RCCI)

Hubungan antara Tumor Infiltrating Lymphocytes (TIL), ekspresi Programmed Death-Ligan 1 (PD-L1) pada sel tumor dan TIL dengan kedalaman invasi pada karsinoma urotelial kandung kemih tipe tidak spesifik di RSUP Sanglah, Bali, Indonesia Luh Ayu Widayanti; I Gusti Ayu Sri Mahendra Dewi; Herman Saputra; Anak Agung Ayu Ngurah Susraini; Ni Putu Sriwidyani; I Made Muliarta
Intisari Sains Medis Vol. 11 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Background: Urotelial carcinoma is one of carcinoma with increasing morbidity and mortality. The depth of invasion is one of risk factors that influence recurrence and survival rate in urotelial carcinoma. Patient with deeper invasion has poorer prognosis in recurrence and survival rate. Generally, carcinoma have microenvironment such as tumor infiltrating lymphocytes (TIL) to eliminate the tumor cells, in proliferative area. Cancer cells can avoid host immunity through PD-1/PD-L1 pathway. This study aimed to determine the association between TIL and PD-L1 expression with the depth of invasion of urotelial carcinoma.Methods: This study was a cross sectional analytic study with 37 samples size. The samples were taken from paraffin block of patients with urotelial bladder carcinoma non-specific type, that has performed resection and histopathological examination at Anatomical Pathology Laboratory Sanglah Hospital Denpasar, from January 1st, 2015 to December 31th, 2019. Rediagnosis was carried out to assess the histopathological diagnosis, TIL and depth of invasion. Then PD-L1 immunohistochemistry was performed. Data were analyzed by Chi-Square test with p<0.05. Data were analyzed using SPSS version 20 for Windows.Results: In this study on bladder urothelial carcinoma non-specific type at Sanglah Hospital Denpasar, we found there is no association between TIL and the depth of invasion (p=0.471). There is association between PD-L1 expression in tumor cells with the depth of invasion (p=0.006), OR 7.04 (CI 95% =1.64-30.20). There is association between PD-L1 expression in TIL with the depth of invasion (p=0.001), OR 13.2 (CI 95% = 2.62-66.43).Conclusion: There is association between PD-L1 expression in tumor cells an PD-L1 expresion in TIL with the depth of invasion. Positive expression of PD-L1 in tumor cells and TIL are more likely to become advance invasion. Tumor Infiltrating Lymphocytes (TIL) and PD-L1 expression on TIL can be used as predictive factors for the depth of invasion in urotelial carcinoma. Latar Belakang: Karsinoma urotelial merupakan salah satu karsinoma dengan morbiditas dan mortalitas yang terus meningkat. Salah satu faktor risiko yang mempengaruhi rekurensi dan survival rate pada karsinoma urotelial adalah kedalaman invasi. Invasi yang lebih dalam memiliki prognosis yang lebih buruk. Karsinoma umumnya disertai lingkungan mikro antara lain tumor infiltrating lymphocytes (TIL) yang berfungsi untuk mengeliminasi tumor. Sel kanker memiliki kemampuan untuk menghindari imun host antara lain melalui ikatan PD-1 dengan PD-L1. Tujuan dari penelitian ini untuk mengetahui hubungan antara TIL dan ekspresi PD-L1 dengan kedalaman invasi pada karsinoma urotelial.Metode: Penelitian ini merupakan studi analitik potong lintang dengan besar sampel adalah 37, yang berasal dari blok parafin penderita karsinoma urotelial kandung kemih tipe tidak spesifik yang diperiksa histopatologi di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar dari 1 Januari 2015 sampai 31 Desember 2019. Rediagnosis preparat dilakukan untuk menilai diagnosis histopatologi, TIL serta kedalaman invasi. Kemudian dilakukan pulasan imunohistokimia PD-L1. Data dianalisis dengan uji Chi-Square dengan nilai p<0,05. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian ini menunjukkan bahwa pada karsinoma urotelial kandung kemih tipe tidak spesifik di RSUP Sanglah Denpasar, tidak terdapat hubungan yang signifikan antara TIL dengan kedalaman invasi (p=0,471). Terdapat hubungan yang signifikan antara ekspresi PD-L1 pada sel tumor dengan kedalaman invasi (p=0,006) dengan OR 7,04 (IK 95% =1,64-30,20), antara ekspresi PD-L1 pada TIL dengan kedalaman invasi (p=0,001) dengan OR 13,20 (IK 95% = 2,62-66,43).Kesimpulan: Terdapat hubungan antara ekspresi PD-L1 pada sel tumor dan pada TIL dengan kedalaman invasi pada karsinoma urotelial kandung kemih tipe tidak spesifik di RSUP Sanglah Denpasar. Ekspresi positif PD-L1 pada sel tumor dan pada TIL memiliki kemungkinan lebih besar untuk menjadi invasi lanjut.

Positive calretinin stain with immunohistochemistry in a 49 year-old female with cardiac myxoma: A case report I Gusti Ayu Sri Mahendra Dewi
Intisari Sains Medis Vol. 11 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Background: Primary tumors of the heart are scarce. Cardiac myxoma is a primary benign heart tumor most often found with an incidence of 0.5-1.0 cases per million population per year and 90% diagnosed in the fourth to seventh decades of life, rarely found in children. Cardiac myxoma is three times more common in women than in men. These tumors are mostly found in the left atrium.Case description: Female, a 49-year-old patient, came with palpitations, shortness of breath, and weakness since the previous six months. On echocardiography, found a mass in the left atrium suspected of a left atrial myxoma. Surgery was performed, and the specimen was sent for histopathological examination. Macroscopically, the tumor mass shows an irregular shape, brownish color, and spongy consistency. Microscopic examination shows the tumor consisting of proliferation of spindle-shaped and stellate cells, with oval to round nuclei, eosinophilic cytoplasm, mostly scattered, some of which are arranged cord and nest, infiltrative between the myxoid stroma and partially surround small blood vessels to form a vasoformative ring. The specimen also contains the proliferation of blood vessels, extravasation of erythrocytes, and the distribution of inflammatory cells and hemosiderophages. The result of the immunohistochemical examination with calretinin was positive.Conclusion: This case was concluded as cardiac myxoma based on clinical features, echocardiography, macroscopic, routine histopathological, and immunohistochemical examinations.

Hubungan antara Stromal Tumor Infiltrating Lymphocytes (TIL) dengan ekspresi Programmed Death-Ligand 1 (PD-L1) pada karsinoma payudara invasif tipe tidak spesifik subtipe Human Epidermal Growth Factor Receptor-2 (HER-2) enriched di RSUP Sanglah, Bali, Ind Popi Imelda Margareth Sitompul; Herman Saputra; Luh Putu Iin Indrayani Maker; I Gusti Ayu Sri Mahendra Dewi; Ni Putu Sriwidyani; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Breast cancer is the most common malignancy cancer in women. This malignancy is molecularly heterogeneous, where overexpression of the HER-2 growth factor receptor generally has a more aggressive nature. The role of immune system interaction, namely Tumor Infiltrating Lymphocytes (TIL) and Programmed Death-Ligand 1 (PD-L1) expression, is associated with prognostic and predictive factors. Therefore, this study aims to investigate the association between stromal TIL with PD-L1 expression in invasive breast carcinoma non-specific type HER-2 enriched subtype.Methods: This study was an observational analytical study with cross-sectional design from January 1st 2015 to December 31st 2019. The samples were taken from a paraffin block of patients with invasive breast carcinoma non-specific type, that has performed histopathological examination, ER, PR, HER-2 and FISH/CISH immunohistochemistry examination at the Anatomical Pathology Laboratory of Sanglah Hospital Denpasar. The samples obtained were stained with PD-L1 immunohistochemistry. The preparation was evaluated to assess TIL. Data were analyzed using SPSS version 20 for Windows.Results: The study found a statistically significant association between stromal TIL with PD-L1 expression in tumor cells (p = 0.018). There is statistically significant association between stromal TIL with PD-L1 expression in TIL (p = 0.001). There is statistically significant association between stromal TIL with PD-L1 expression in total tumor cells and TIL (p = 0.007) in invasive breast carcinoma non-specific type HER-2 enriched subtype at Sanglah Hospital, Denpasar.Conclusion: In conclusion, there is an association between stromal TIL with the expression of PD-L1 in tumor cells and TIL in invasive breast carcinoma non-specific type HER-2 enriched subtype. Latar Belakang: Kanker payudara merupakan keganasan tersering pada wanita. Keganasan ini bersifat heterogen ditinjau dari aspek molekuler, dimana overekspresi HER-2 umumnya memiliki sifat yang lebih agresif. Peranan sistem imun, yakni ekspresi Tumor Infiltrating Lymphocytes (TIL) dan Programmed Death-Ligand 1 (PDL-1) dihubungkan dengan faktor prognostik. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi hubungan antara stromal TIL dengan ekspresi PD-L1 pada karsinoma payudara invasif tipe tidak spesifik subtipe HER-2 enriched.Metode: Penelitian ini merupakan penelitian observasional analitik dengan rancangan studi potong lintang mulai dari 1 Januari 2015 sampai 31 Desember 2019. Sampel adalah blok parafin penderita karsinoma payudara invasif tipe tidak spesifik yang telah dilakukan pemeriksaan histopatologi, imunohistokimia ER, PR, HER-2 dan FISH/CISH di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar. Sampel yang diperoleh dilakukan pulasan imunohistokimia PD-L1 Preparat sampel digunakan untuk menilai TIL. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Pada penelitian ditemukan hubungan yang signifikan secara statistik antara stromal TIL dengan ekspresi PD-L1 pada sel tumor (p=0,018). Terdapat hubungan yang signifikan secara statistik antara stromal TIL dengan ekspresi PD-L1 pada TIL (p=0,001). Terdapat hubungan yang signifikan secara statistik antara stromal TIL dengan ekspresi PD-L1 pada total sel tumor dan TIL (p=0,007) pada karsinoma payudara invasif tipe tidak spesifik subtipe HER-2 enriched di RSUP Sanglah Denpasar.Kesimpulan: Hasil penelitian ini menunjukkan terdapat hubungan bermakna antara stromal TIL dengan ekspresi PD-L1 sel tumor dan TIL pada karsinoma payudara invasif tipe tidak spesifik subtipe HER-2 enriched.

Hubungan antara ekspresi c-MET dengan derajat diferensiasi histologi dan tingkat kedalaman invasi pada infiltrating urothelial carcinoma kandung kemih di RSUP Sanglah, Bali, Indonesia Kadek Agus Suhardinatha Putra; I Gusti Ayu Sri Mahendra Dewi; Herman Saputra; Ni Wayan Winarti; Anak Agung Ayu Ngurah Susraini; Ni Putu Ekawati; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: c-MET is a tyrosine kinase receptor that binds specifically to its ligand, namely hepatocyte growth factor (HGF). c-MET signaling deviations play a role in the progressivity of a wide variety of malignancies including bladder cancer. This study aims to determine the relationship between c-MET expression with the histological differentiation and depth of invasion in infiltrating urothelial carcinoma of bladder.Methods: This study was a cross-sectional analytical study with 42 sample sizes. The samples were taken from a paraffin block of patients with infiltrating urothelial carcinoma of the bladder, that has performed resection and histopathological examination who were examined at the Anatomical Pathology Laboratory of Sanglah Hospital Denpasar from 2015-2020. The c-MET expression was examined by immunohistochemical staining of c-MET and evaluated using the H-score method. Data were analyzed using SPSS version 20 for Windows.Results: Fisher's Exact test showed a statistically significant association between c-MET expression and the histological differentiation grade (p = 0.000). There was also a significant association between c-MET expression and depth of invasion with the Chi-Square test (p = 0.016), the Prevalence Ratio (PR) was 2.0 (95% CI = 1.2-3.6).Conclusion: In conclusion, there is an association between c-MET expression with the histological differentiation grade and depth of invasion in the infiltrating urothelial carcinoma of bladder. High c-MET expression had twice the risk of developing advanced invasion. Latar Belakang: c-MET merupakan reseptor tirosin kinase yang berikatan secara spesifik dengan ligannya, yaitu faktor pertumbuhan hepatosit (HGF). Penyimpangan c-MET signaling diketahui berperan dalam agresivitas berbagai macam keganasan termasuk kanker kandung kemih. Penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi c-MET dengan derajat diferensiasi histologi dan tingkat kedalaman invasi pada infiltrating urothelial carcinoma kandung kemih.Metode: Penelitian ini merupakan studi analitik potong lintang dengan besar sampel adalah 42, yang berasal dari blok parafin penderita infiltrating urothelial carcinoma kandung kemih yang diperiksa histopatologi di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar dari 2015-2020. Ekspresi c-MET diperiksa dengan pengecatan imunohistokimia c-MET dan dievaluasi menggunakan metode H-score. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Uji Fisher’s Exact menunjukkan hubungan yang bermakna secara statistik antara ekspresi c-MET dengan derajat diferensiasi histologi (p=0,000). Didapatkan juga hubungan yang bermakna antara ekspresi c-MET dengan tingkat kedalaman invasi dengan uji Chi-Square (p=0,016), prevalence ratio 2,0 (IK 95%=1,2-3,6).Kesimpulan: Sebagai simpulan terdapat hubungan antara ekspresi c-MET dengan derajat diferensiasi histologi dan tingkat kedalaman invasi pada infiltrating urothelial carcinoma kandung kemih. Ekspresi c-MET tinggi memiliki risiko dua kali lebih besar mengalami invasi lanjut. c-MET diharapkan dapat menjadi faktor prognostik yang bermanfaat dalam penatalaksanaan infiltrating urothelial carcinoma kandung kemih.

Hubungan antara ekspresi Vascular Cell Adhesion Molecule-1 (VCAM-1) dengan tipe dan stadium T karsinoma epitel permukaan ovarium di RSUP Sanglah, Bali, Indonesia I Made Wirya Sastra; Luh Putu Iin Indrayani Maker; Anak Agung Ayu Ngurah Susraini; I Wayan Juli Sumadi; I Gusti Ayu Sri Mahendra Dewi; Herman Saputra; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Vascular Cell Adhesion Molecule-1 (VCAM-1) is an important protein in oncogenesis, angiogenesis, tumor development, and metastasis. Studies regarding the expression of VCAM-1 in ovarian carcinoma have found both supportive and contradictory results. Therefore, this study evaluates the relationship between VCAM-1 expression and the type and stage of ovarian surface epithelial carcinoma at Sanglah General Hospital, Bali, Indonesia.Methods: This study was a cross-sectional analytical study with a sample size of 36, which came from paraffin block of patients with ovarian surface epithelial carcinoma who were examined histopathologically at the Anatomical Pathology Laboratory of Sanglah Hospital Denpasar from 1 January 2017 to 31 December 2019. Samples are grouped into type I and type II, then the stage I or II and III groups. After that, the VCAM-1 immunohistochemical streak was performed to assess high or low expression in these groups and analyzed its relationship with the type and stage T of ovarian surface epithelial carcinoma. Data were analyzed using SPSS version 25 for Windows.Results: The analysis results using the Chi-Square test showed a statistically significant relationship (p=0.001) between the VCAM-1 expression with type and stage surface epithelial ovarian carcinoma (p=0.007). The results of the mean difference test are 0.48 (95% CI=0.33-0.70) and showed a statistically significant difference (p=0.001) between VCAM-1 expression with a type of ovarian carcinoma.Conclusion: In conclusion, there is an association between expression VCAM-1 with the type of surface epithelial ovarian carcinoma and at a stage, with a 2.3 times higher probability of VCAM-1 expression in the stage III group than in stage I or II group. Latar Belakang: Vascular Cell Adhesion Molecule-1 (VCAM-1) merupakan salah satu protein yang penting dalam onkogenesis, angiogenesis, perkembangan tumor dan metastasis. Penelitian-penelitian mengenai ekspresi VCAM-1 pada karsinoma ovarium mendapatkan hasil yang pro dan kontra. Oleh karena itu, penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi VCAM-1 dengan tipe dan stadium T karsinoma epitel permukaan ovarium di RSUP Sanglah, Bali, Indonesia.Metode: Penelitian ini merupakan studi analitik potong lintang dengan besar sampel adalah 36, yang berasal dari dari blok parafin penderita karsinoma epitel permukaan ovarium yang diperiksa secara histopatologi di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar dari 1 Januari 2017 sampai 31 Desember 2019. Sampel dikelompokkan menjadi tipe I dan tipe II, kemudian kelompok stadium I atau II dan III. Setelah itu dilakukan pulasan imunohistokimia VCAM-1 untuk menilai ekspresi tinggi ataupun rendah pada kelompok-kelompok tersebut dan dianalisis hubungannya dengan tipe dan stadium T karsinoma epitel permukaan ovarium. Data dianalisis dengan uji Chi-Square dengan nilai p <0,05.Hasil: Hasil penelitian menunjukkan terdapat hubungan yang bermakna secara statistik (p=0,001) antara ekpresi VCAM-1 dengan tipe karsinoma dan stadium T (p=0,007). Hasil uji beda rerata adalah 0,48 (IK 95%=0,33-0,70) dan menunjukkan perbedaan bermakna secara statistik (p=0,001) antara ekspresi VCAM-1 dengan tipe karsinoma ovarium.Kesimpulan: Sebagai simpulan, terdapat hubungan antara ekspresi VCAM-1 dengan tipe karsinoma epitel permukaan ovarium dan pada stadium, dengan kemungkinan 2,3 kali terjadi ekspresi VCAM-1 yang tinggi pada kelompok stadium III dibandingkan stadium I atau II.

Perbedaan ekspresi Vascular Endothelial Growth Factor (VEGF) pada meningioma risiko rendah dan risiko tinggi di RSUP Sanglah, Bali, Indonesia Ivana Juliarty Sitanggang; Ni Putu Sriwidyani; I Wayan Juli Sumadi; I Gusti Ayu Sri Mahendra Dewi; Luh Putu Iin Indrayani Maker; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Vascular Endothelial Growth Factor (VEGF) is an angiogenic factor that plays an important role in tumor angiogenesis. VEGF in meningioma is up-regulated and indicates its role as a proangiogenic factor. It has an association with tumor recurrence and progression. This study aims to determine the differences in VEGF expression in low-risk and high-risk meningiomas at Sanglah General Hospital, Bali, Indonesia.Methods: This study was a cross-sectional analytical study with a sample size of 52, which came from meningioma patients examined histopathologically at the Anatomical Pathology Laboratory of Sanglah General Hospital from January to December 2019. The VEGF immunohistochemical staining was performed and interpreted using Histo score (H-score). VEGF expression was categorized into high and low expression, with the cut-off value determined based on the median value. Data were analyzed using SPSS version 20 for Windows.Methods: The results showed that out of 52 meningioma samples, 37 (71.1%) cases of low-risk meningiomas with low VEGF expression, 6 cases (11.5%) of low-risk meningiomas with high VEGF expression, and 9 cases (17.3%) of high-risk meningiomas with high VEGF expression. There was no high-risk meningioma with low VEGF expression. There was a significant difference in VEGF expression between the low-risk and high-risk meningioma groups (p = 0.00), and high VEGF expression had a prevalence risk for the incidence of high-risk meningioma by 2.5 times (95% CI=1.3-4.6).Conclusion: Based on the results of this study, it was concluded that there was a VEGF expression difference between low-risk and high-risk meningiomas in Sanglah General Hospital Denpasar and high VEGF had a prevalence risk for the occurrence of high-risk meningiomas by 2.5 times. Latar Belakang: Vascular Endothelial Growth Factor (VEGF) merupakan faktor angiogenik yang berperan penting dalam angiogenesis tumor. VEGF pada meningioma mengalami up-regulation yang menunjukkan perannya sebagai faktor proangiogenik yang berkaitan dengan rekurensi dan perkembangan tumor. Penelitian ini bertujuan untuk menentukan perbedaan ekspresi VEGF pada meningioma risiko rendah dan risiko tinggi di RSUP Sanglah, Bali, Indonesia.Metode: Penelitian ini merupakan studi analitik potong lintang dengan besar sampel adalah 52, yang berasal dari blok parafin penderita meningioma yang diperiksa histopatologi di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar dari Januari - Desember 2019. Dilakukan pulasan imunohistokimia VEGF dan penilaiannya menggunakan Histo score (H-score). Ekspresi VEGF dikategorikan menjadi tinggi dan rendah dengan nilai cut-off yang ditentukan berdasarkan nilai median. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian ini menunjukkan bahwa dari 52 sampel meningioma, terdapat 37 kasus (71,1%) meningioma risiko rendah dengan ekspresi VEGF rendah, 6 kasus (11,5%) meningioma risiko rendah dengan ekspresi VEGF yang tinggi, dan 9 kasus (17,3%) meningioma risiko tinggi dengan ekspresi VEGF tinggi. Tidak didapatkan meningioma risiko rendah dengan ekspresi VEGF rendah. Didapatkan perbedaan ekspresi VEGF yang bermakna antara kelompok meningioma risiko rendah dan risiko tinggi (p=0,00) dan ekspresi VEGF tinggi mempunyai risiko prevalensi untuk terjadinya meningioma risiko tinggi sebesar 2,5 kali (95% IK=1,3-4,6).Kesimpulan: Berdasarkan hasil penelitian ini didapatkan perbedaan ekspresi VEGF antara meningioma risiko rendah dan risiko tinggi di RSUP Sanglah Denpasar dan pada VEGF tinggi mempunyai risiko prevalensi untuk terjadinya meningioma risiko tinggi sebesar 2,5 kali.

Hubungan Lymph Vessel Density (LVD) dengan invasi limfatik dan metastasis kelenjar getah bening regional pada karsinoma payudara invasif tipe tidak spesifik di RSUP Sanglah, Bali, Indonesia Katrin Rotua Simbolon; I Gusti Ayu Sri Mahendra Dewi; Ni Putu Sriwidyani; Luh Putu Iin Indrayani Maker; Herman Saputra; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Lymph Node Metastasis (LNM) is considered the most important prognostic factor in breast cancer. The progress of lymphatic metastasis is thought to involve the proliferation of lymphatic vessels (lymphangiogenesis), Lymph Vessel Invasion (LVI), and lymph node metastasis step by step. This study aims to evaluate the association between LVD with LVI and regional LNM in invasive breast carcinoma of no special type.Methods: This study used a cross-sectional analytical study, using a sample of 38 paraffin-embedded tissue from the patient with invasive breast carcinoma of no special type in Sanglah General Hospital, Bali, Indonesia, in 2018. The sample will be used to assess LVD and LVI by D2-40 immunohistochemistry. Expression of D2-40 with vigorous-intensity was evaluated on the membrane and cytoplasm of lymphatic endothelial cells to determine the definition of lymphatic vessels. Lymph vessel density was determined by counting the number of lumens of peritumoral lymphatic vessels in five fields of view with the densest lymphatic vessel lumen using a Leica microscope (DM750, 400x, area 0.225mm2). Data were analyzed using SPSS version 24 for Windows.Results: The LVD cut-off value is 16.5 lymphatic vessel lumens/0.225 mm2 (65.4% for sensitivity and area Under the Curve (AUC)=70.2%). Then cases were classified into the category of high LVD (?16,5 lymphatic vessel lumen/0,225 mm2) and low LVD (<16.5 lymphatic vessels lumen/0,225 mm2). The study found a statistically significant association between LVD and lymph vessel invasion (PR=3.6; p=0.043; 95%CI=0.88-14.78) and regional lymph node metastases (PR =1.7; p=0.02; 95%CI=1.03-2.79).Conclusion: This study proved an association between LVD with lymph vessel invasion and regional lymph node metastases. Latar Belakang: Metastasis Kelenjar Getah Bening (KGB) dianggap sebagai faktor prognostik yang paling penting pada kanker payudara. Perkembangan metastasis limfatik diduga melibatkan proliferasi pembuluh limfatik (limfangiogenesis), Lymph Vessel Invasion (LVI) atau invasi limfatik, dan metastasis kelenjar getah bening secara bertahap. Penelitian ini bertujuan untuk mengevaluasi hubungan antara Lymph Vessel Density (LVD) dengan invasi limfatik dan metastasis kelenjar getah bening regional pada karsinoma payudara invasif tipe tidak spesifik.Metode: Penelitian ini menggunakan metode analitik potong lintang dengan sampel 38 blok parafin dari pasien karsinoma payudara invasif tipe tidak spesifik di RSUP Sanglah, Bali, Indonesia tahun 2018. Penilaian LVD dan LVI dengan pulasan imunohistokimia D2-40. Ekspresi D2-40 dengan intensitas yang kuat dievaluasi pada membran dan sitoplasma sel endotel limfatik untuk menetapkan definisi pembuluh limfatik. Lymph vessel density ditentukan dengan menghitung jumlah lumen pembuluh limfatik peritumoral pada lima lapangan pandang yang memiliki lumen pembuluh limfatik terpadat, menggunakan mikroskop Leica (DM750, 400x, luas area 0,225mm2). Data dianalisis dengan SPSS versi 24 untuk Windows.Hasil: Nilai cut-off LVD adalah 16,5 lumen pembuluh limfatik/0,225 mm2 (sensitivitas 65,4% dan Area Under the Curve (AUC)=70,2%). Kemudian kasus dikategorikan menjadi LVD tinggi (?16,5 lumen pembuluh limfatik / 0,225 mm2) dan LVD rendah (<16,5 lumen pembuluh limfatik / 0,225 mm2). Hasil analisis statistik menunjukkan bahwa terdapat hubungan antara LVD dengan invasi limfatik (PR=3,6; p=0,043; IK 95%=0,88-14,78) dan metastasis KGB regional (PR=1,7; p=0,02; IK 95%=1,03-2,79).Kesimpulan: Penelitian ini membuktikan bahwa terdapat hubungan antara LVD dengan invasi limfatik dan metastasis KGB regional.

Co-Authors Anak Agung Ayu Ngurah Susraini and I G. Alit-Artha annie minerva datui Desak Made Cittarasmi Saraswati Seputra Desak Putu Gayatri Saraswati Seputra Desak Putu Gayatri Saraswati Seputra Desak Putu Gayatri Saraswati Seputra Diah Widityasari, Diah E. Joewarini Efrisca M Br Damanik Firman Parulian Sitanggang Gde Ngurah Idraguna Pinatih Herman Saputra Herman Saputra Herman Saputra Herman Saputra I Gusti Alit Artha I Gusti Bagus Lulut Premana Mulia I Gusti Bagus Mulia Agung Pradnyaandara I Gusti Ngurah Bagus Rai Mulya Hartawan I Kadek Adi Purnama Sandhi I Ketut Suwiyoga I Made Gotra I Made Muliarta I Made Muliarta I MADE MULIARTA . I Made Naris Pujawan I Made Wirya Sastra I Wayan Juli Sumadi I Wayan Juli Sumadi IB Caka Gunantara Ida Ayu Eugenia Natasha Blanco Oka Ida Ayu Ista Nariswari Ida Ayu Jelantik Astuti Ida Ayu Krisna Cantika Dewi Ida Ayu Meilasari Dewi Ida Bagus Ardya Kurnia Wilananda Ivana Juliarty Sitanggang Jovi Carina Handoko Kadek Agus Suhardinatha Putra Kadek Dwi Pradnyawati kadek pramesti dewi Katrin Rotua Simbolon Luh Ayu Widayanti Luh putu Iin Indrayani Luh Putu Iin Indrayani Maker Made Dwi Hartayati Moestikaningsih Moestikaningsih Ni Ketut Ari Widhiasih, Ni Ketut Ari Ni Made Mahastuti Ni Putu Ekawati Ni Putu Sriwidyani Ni Wayan Winari Ni Wayan Winarti Nur Silfiah Nyoman Srie Laksminingsih Popi Imelda Margareth Sitompul Putu Ayu Widya Pramesti Putu Ayu Winda Wirastuti Giri Putu Dony Astika Wiguna Putu Risky Yoga Pradnyana Shameni Subramaniam Silvia Nuhyil Indriani Sylvi Diahningrum Tjandra Kristiana Volman Tampubolon

Title Search

Found 11 Documents Search Journal : ISM (Intisari Sains Medis) : Jurnal Kedokteran

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 11 Documents
Search
Journal : ISM (Intisari Sains Medis) : Jurnal Kedokteran