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All Journal Pastura; Journal Of Tropical Forage Science LAW REFORM MIMBAR (Jurnal Sosial dan Pembangunan) Jurnal Bisnis dan Manajemen BAHAS Jurnal Agroekoteknologi Journal of Agro Complex Compiler YUME : Journal of Management Performance Jurnal Manajemen Maranatha JBIMA (Jurnal Bisnis dan Manajemen) Jurnal Agroplasma Civilla : Jurnal Teknik Sipil Universitas Islam Lamongan Jurnal Teknologi dan Riset Terapan (JATRA) Dinamika Kerajinan dan Batik : MAJALAH ILMIAH Lontara: Journal of Health Science and Technology PERTANIAN TROPIK Jurnal Ekonomi, Bisnis, dan Akuntansi
Sumarsono Sumarsono
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Journal : Pastura

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ANALYSIS AND EXPRESSION OF AL-TOLERANT GENES FROM SOYBEAN [Glycine max (L.) Merryl] ON FORAGE CROPS AND Escherichia coli S. Anwar; Sumarsono Sumarsono; F. Kusmiyati
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 3 No 2
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.015 KB) | DOI: 10.24843/Pastura.2014.v03.i02.p04

Abstract

n order to analyze and to study expressions of the Al-tolerant genes, we have examined five clone genes that were isolated from soybean cv. Lumut. Soybean cv. Lumut and Slamet, Centrocema pubescens, Pennisetum purpureum and Escherichia coli were selected for futher analysis. Based on the DNA sequencing, searching enzyme restriction sites and searching DNA homology with the genebank database; the clones encoding: (1) Catalase (gmali12, that function as an antioxidant), (2) Proliferating cell nuclear antigen like protein/PCNALP (gmali15, that involved as one of transcriptional regulator in the eucaryotic cell cycle), (3) Growth hormone (gmali22, this gene may play a role on stimulation of cell growth/development), (4) Amine oxidase (gmAO, genebank accession number AF313622, a gene that function as amine oxidation and/or antioxidant), and (5) Aminoacyl peptidase (gmAP, genebank accession number AF091304, a serine protease gene). Expressions of the clone genes either on forage crops or Escherichia coli indicated that all of the clones are basic genes, but its expression increased with aluminium induction (Al-induced genes) and involved in detoxification to Al stress. From this research, we also found similar responses between oxidative stress and Al stress to gene responses.
Co-Authors Adriani Darmawati AINUR ROFIQ Akbar Maulana Tirta Anton Setiawan Honggowibowo apitya muktifani Atim Djazuli Baharuddin Ali Cakra Wijaya Kusuma Rahadi Didik Wisnu Widjajanto Dita Nafa Anggraeni Dwi Nugraheny Dwita Darmawati Dwiwiyati Astogini Elizabeth Nurmiyati Tamatjita Endyatna Puthut Bagus Pratama F. Kusmiyati Firwan Tan Gamal Kartono Handoyo Handoyo Hanif Abdul Latif Hasmin Tamsah Hendra Nurahmanto Herlinda Mahdania Harun Holyness Nurdin Singadimedja Indra Usmanan Kurnia Rizki Novianto Mario Agapito Arizald Gobel Mattalatta Mattalatta Maya Suhayati Muhamad Ali Mudhofar Musdalifah Musdalifah Neli Afrilliana Novrianto novrianto Nugroho, Meriana Wahyu Nur Aini Nurcahyani Dewi Retnowati Rahma Khairunisa Rahmadun Barokah RETNO WIDURI Rivcky Septianto Rudi Febriamansyah S. Anwar Safira Amalia Hapsari Saryanto Saryanto Sri Murni Setyawati Sudirman Sudirman Sutarwadi Sutarwadi Syamsul Hidayatullah Wintolo, Hero Yayat Giyatno Yoga Yuniadi Yuliani Indrianingsih