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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Hortikultura Indonesia Jurnal Tanaman Industri dan Penyegar Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian JURNAL AGRONIDA JURNAL CITRA WIDYA EDUKASI

Sukma, Dewi

Departemen Agronomi Dan Hortikultura – Fakultas Pertanian- Institut Pertanian Bogor

Author-ID : 2432086

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering

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Induksi Ginogenesis melalui Kultur Multi Ovule Slice dan Kultur Ovary Slice Dianthus chinensis Suskandari Kartikaningrum; Agus Purwito; Gustaaf Adolf Wattimena; Budi Marwoto; Dewi Sukma
Indonesian Journal of Agronomy Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Callus induction was studied in five genotypes of Dianthus chinensis using 2.4 D and NAA. Calluses can be obtainedfrom unfertilized ovule culture and ovary culture. The aim of the research was to study gynogenic potential and responseof Dianthus chinensis through ovule slice and ovary slice culture for obtaining haploid plants. Five genotypes of Dianthuschinensis and five media were used in ovule slice culture and two genotypes and three medium were used in ovary culture.Flower buds in the 7th stage were incubated for the purpose of dark pre-treatment at 4 oC for one day. Ovules and ovaries wereisolated and cultured in induction medium. Cultures were incubated for the purpose of dark pre-treatment at 4 oC for seven days, followed by 25 oC light incubation. The result showed that 2.4D was better than NAA in inducing callus. Percentage of regenerated calluses were produced in V11, V13 and V15 genotypes in M7 medium (MS + 2 mg L-1 2.4D + 1 mg L-1 BAP + 30 g L-1 sucrose and M10 medium (MS + 1 mg L-1 2.4D + 1 mg L-1 BAP + 20 g L-1 sucrose). All calluses originated from ovule and ovary cultures flowered prematurely. Double haploid (V11-34) were obtained from ovule slice culture based on PER (peroksidase) and EST (esterase) isoenzym marker.Keywords: ovule slice culture, ovary slice culture, callus, Dianthus sp., haploid

Induksi Poliploidi Phalaenopsis amabilis (L.) Blume dan Phalaenopsis amboinensis J. J. Smith dengan Kolkisin dalam Kultur In Vitro Eka Martha Della Rahayu; Dewi Sukma; Muhamad Syukur; rawati ,
Indonesian Journal of Agronomy Vol. 43 No. 3 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTPhalaenopsis amabilis (L.) Blume and Phalaenopsis amboinensis J.J. Smith (diploid) are important in Phalaenopsis breeding. Polyploid species are needed for crossing with polyploid hybrid varities of Phalaenopsis. The objectives of this study were to obtain effective concentration of colchicine to induce polyploidy and to produce polyploid plantlets of P. amabilis and P. amboinensis. Experiment was arranged in randomized complete block design with one factor, the colchicine concentration. Protocorms of P. amabilis and P. amboinensis were immersed in half strength of Murashige-Skoog (1/2 MS) liquid media added with colchicine (0; 0.5; 5; 25; 50, and 75 mg L-1) for 10 days. The results showed that higher concentration of colchicine on both species did not have significant effect on the survival of the plantlets at 24 weeks after treatment. The average number of leaves and roots of colchicine treated planlets from both species were less than the control plantlets. Immersing protocorm in colchicine at concentration of 50 mg L-1 for 10 days was effective in inducing polyploid plantlets of P. amabilis and P. amboinensis with the frequency of 33.3% and 40%, respectively. Polyploid plantlet has larger stomata size and lower stomata density than the diploid ones.Keywords: chromosome number, colchicine, polyploid, protocorm, stomatal density, stomatal size

Perubahan Morfologi dan Pertumbuhan Handeuleum (Graptophyllum pictum L. Griff) akibat Iradiasi Sinar Gamma Arrin Rosmala; Nurul Khumaida; Dewi Sukma
Indonesian Journal of Agronomy Vol. 43 No. 3 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTHandeuleum (Graptophyllum pictum L.Griff) is a medicinal plant widely used as a traditional medicine due to its benefecial content. Therefore, it should be developed as one of the leading Indonesian medicinal plants. The purpose of this research was to study the effect of gamma ray irradiation on morphological alteration and growth of Handeuleum accession from Bogor. The doses of gamma rays were 0, 15, 30, 45, 60, 75, 90, and 105 Gy. The results of the research showed that gamma-ray irradiation induced morphological changes and influences the growth of Handeuleum. Irradiation dose at rate of 105 Gy produced new leaf morphology in Handeuleum, namely cordate. Doses of 60, 75, 90, and 105 Gy produce stunted plants with stiff and yellowish-green leaves. The dose of 45 Gy produced relative green color index which was higher than the control plants.Keywords: handeuleum, gamma irradiations

Analisis Keragaman Morfologi dan Biokimia pada Anggrek Phalaenopsis (Orchidaceae) Aline Sisi Handini; Dewi Sukma; dan Sudarsono
Indonesian Journal of Agronomy Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTPhalaenopsis orchid is one of famous genera in Orchidaceae family which have high economic value. Phalaenopsis breeding needs to be developed intensively to fulfill market demand for new varieties. The objective of this study was to obtain the information of morphological and biochemical diversity of a collection of Phalaenopsis orchid which will be useful for Phalaenopsis breeding. Plant materials used were 10 genotypes of Phalaenopsis with various petal colours of white, yellow, pink, deep pink, purple, or combination of yellow and brown. Morphological diversity of 10 genotypes were analyzed based on qualitative and quantitative characters following UPOV guidance for Phalaenopsis, while biochemical diversity was based on pigment content such as chlorophyl, anthocyanin, and carotene in plant roots, leaves and flower petals. The result of this research showed morphological diversity of 10 genotypes used was relatively high, with similarity of 55%. In that level of similarity, genotypes were grouped into two cluster. Biochemical analysis showed that most of genotypes were different for anthocyanin and carotenoid content for each plant organ. Genotype which showed high content of anthocyanin in leaves was Phal. hybrid ‘PH37’ while high content of carotene in leaves was Phal. hybrid ‘PIROUI. High content of anthocyanin in petals were found in Phal. hybrid ‘PH37’ and Phal. esmeralda, and high content of carotene were in Phal. cornucervi and Phal. cornucervi Red. Root with high anthocyanin content was found in Phal. hybrid ‘PIROU1’. There were significant correlation (0.975) for anthocyanin content between leaves and petals, petals and roots (0.953) on Phal.hybrid ‘PH37’, and between leaves and roots (0.874) on Phal. hybrid ‘PIROU1).Keywords: anthocyanin, carotene, chlorophyll, Phalaenopsis, similarity

Morfologi dan Pertumbuhan Planlet Hasil Induksi Poliploidi melalui Perlakuan Kolkisin pada Kuncup Bunga Anggrek Bulan (Phalaenopsis amabilis (L.) Blume) Tubagus Kiki Kawakibi Azmi; Dewi Sukma; Sandra Arifin Aziz; dan Muhamad Syukur
Indonesian Journal of Agronomy Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Induction of polyploid gametes is one of useful plant polyploidization methods. Some of its benefits are to obtain triploid and tetraploid progenies at the same time by cross and self pollination. Previous research showed that some morphological characters which could be the indications of polyploidy plantlets before the analysis of chromosome number. Colchicine treatment on flower bud of diploid Phalaenopsis amabilis was conducted to determine the effect of colchicine on flower bud development, plantlets morphology and growth, and potential of polyploidy induction based on plantlets morphology. Colchicine concentrations in the experiment were 0, 50, 500, 1,000, and 2,000 mg L-1, with three days duration of treatments with aluminium foil wraps on flower buds. The results showed that high colchicine concentrations (2,000 mg L-1) inhibited flowers blooming of treated flower buds. Based on morphological characters, plantlets were classified into normal and putative polyploid plantlets. Putative polyploid plantlets from colchicine with the concentration of 50, 500, and 1,000 mg L-1 were 71.2, 86.4, and 100.0% respectively.Keywords: colchicine concentration, morphological characters, normal plantlets, putative polyploidy, reproductive organ

Uji Ketahanan Anggrek Hibrida Phalaenopsis terhadap Penyakit Busuk Lunak yang Disebabkan oleh Dickeya dadantii Refa Firgiyanto; Sandra Arifin Aziz; Dewi Sukma; dan Giyanto
Indonesian Journal of Agronomy Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTOne of the most popular orchids and cultivated by Indonesia and other countries is Phalaenopsis. The main disease of Phalaenopsis orchids in Indonesia is soft rot caused by bacteria Dickeya dadantii. The purpose of this study was to know the resistanctcy of Phalaenopsis hybrid to soft rot disease. The experiment was conducted at the Bacterial Plant Laboratory, Department of Plant Protection, the Greenhouse of Leuwikopo, Bogor Agricultural University, and the plastic house of Alam Sinar Sari Dramaga, Bogor from June 2014 to February 2015. The experimental design was randomized block design with three replications. Five genotypes of hybrid Phalaenopsis were tested, namely Phal. KHM 205, Phal. KHM 1126, Phal. KHM 1318, Phal. AMP 17, and Phal. KHM 2249. Phal. amabilis, Phal. esmeralda, Phal. amboinensis, and Phal. cornu-cervi were used as controls. The resistance testing was performed by inoculating bacteria D. dadantii on leaves of the orchids. The results showed all Phalaenopsis hybrid showed disease symptoms after inoculation. Phal. KHM 2249 had the lowest number of fallen leaves and the highest number of survive plants compared to the other hybrid Phalaenopsis. Survival rate was likely related to peroxidase activity and leaf thickness.Keywords: fallen leaves, leaf thickness, peroxidase, Phalaenopsis

Respon Plantlet In Vitro dan Induksi Ketahanan Bibit Phalaenopsis amabilis terhadap Dickeya dadantii Menggunakan Asam Salisilat Uchti Nuzul Qhinanti Lubis; Dewi Sukma; Sudarsono
Indonesian Journal of Agronomy Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Phalaenopsis merupakan salah satu anggrek yang terkenal karena keindahan bunganya dengan variasi warna yang tinggi dan periode pembungaan yang lama. Salah satu masalah dalam budidaya anggrek adalah Penyakit Busuk Lunak (PBL) yang disebabkan oleh bakteri Dickeya dadantii. Induksi resistensi dengan Asam Salisilat (AS) merupakan metode alternatif dalam mengendalikan PBL pada Phalaenopsis. Penelitian ini dilaksanakan pada bulan November 2018 sampai Maret 2019, di Laboratorium Kultur Jaringan Tanaman, Departemen Agronomi dan Hortikultura IPB. Penelitian terdiri dari dua percobaan. Percobaan 1 bertujuan untuk mengevaluasi keragaman respon planlet in vitro asal biji hasil penyerbukan sendiri P. amabilis terhadap D. dadanti dengan inokulasi pada daun terpisah dan menentukan tingkat ketahanannya. Sebanyak 7 dari 158 planlet yang diuji, memiliki respon agak tahan terhadap D. dadantii, sedangkan sisanya rentan atau sangat rentan. Percobaan 2 bertujuan untuk membandingkan respon bibit P. amabilis terhadap D. dadantii pada 1 hari setelah perlakuan asam salisilat (AS) pada konsentrasi 0, 15, 30, 45, 60, 75, dan 90 ppm menggunakan metode inokulasi daun pada bibit yang masih menempel di tanaman. Percobaan 2 menggunakan rancangan acak kelompok lengkap teracak (RKLT). Hasil penelitian menunjukkan perlakuan AS 75 ppm paling baik dalam menginduksi ketahanan bibit P. amabilis terhadap patogen penyebab busuk lunak D. dadantii. Tanaman pada perlakuan tersebut menghasilkan nilai CFU dan AUPDC lebih rendah dan aktivitas peroksidase lebih tinggi dibanding perlakuan lainnya. Kata kunci: daun terpisah, daun pada tanaman, keparahan penyakit, metode inokulasi, penyakit busuk lunak

Isolasi dan Karakterisasi Potongan DNA Gen Sterol Metiltransferase 1 (SMT1) Asal Kelapa Sawit Syamsi Rizal; Dewi Sukma; Roberdi Siberakuno; Tony Liwang; Sudarsono
Indonesian Journal of Agronomy Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Tanaman dengan pertambahan tinggi yang lambat merupakan karakter penting dalam program pemuliaan kelapa sawit dan brasinosteroid merupakan hormon penting yang berpengaruh untuk karakter tersebut. Gen sterol metiltransferase 1 (SMT1) merupakan salah satu gen kunci dalam biosintesis brasinosteroid. Pengembangan marker molekuler dapat dimulai dengan identifikasi dan karakterisasi gen yang terkait dengan karakter target. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi keragaman nukleotida gen SMT1. Amplifikasi PCR dilakukan menggunakan genom Elaeis oleifera (E.o.), E. guineensis (E.g.) dan hibrida dari keduanya (E.g. x E.o.) dengan sepasang primer spesifik gen SMT1. Dalam penelitian ini berhasil diamplifikasi satu potongan DNA gen SMT1 dan dari hasil DNA sequencing berhasil diidentifikasi sebanyak 501- 505 pb. Potongan DNA yang diidentifikasi terdiri atas partial intron 8, exon 9, intron 9, dan patial exon 10, yang menyandi antara 104-105 residu asam amino. Domain residu asam amino terkonservasi ditemukan dalam hasil translasi amplikon, yang berkorelasi dengan situs aktif dalam biosintesis brasinosteroid dan mengkonfirmasi identitas amplikon sebagai bagian dari gen SMT1. Keragaman nukleotida potongan DNA gen SMT1 yang teridentifikasi berpotensi dapat digunakan untuk pengembangan marker molekuler yang bermanfaat untuk pemuliaan tanaman kelapa sawit, terutama untuk karakter pertambahan tinggi tanaman yang lambat. Kata kunci: Brasinosteroid, sterol, tanaman kerdil

Pemberian Ekstrak Rebusan Daun Sirih Sebagai Pengganti Perak Nitrat Dalam Larutan Pengawet Bunga Potong Dendrobium ‘Sonia’ Yane Riana Putri; Dewi Sukma
Buletin Agrohorti Vol. 7 No. 1 (2019): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Percobaan dilaksanakan pada April sampai Juni 2014 di Laboratorium Pasca Panen dan Ruang Forum Pasca Sarjana IPB, Dramaga, Bogor. Tujuan penelitian ini untuk mendapatkan komposisi ekstrak rebusan daun sirih yang tepat sebagai anti mikroba pengganti perak nitrat dalam larutan pengawet anggrek potong Dendrobium ‘Sonia’. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) dengan faktor tunggal komposisi larutan pengawet. Terdapat 5 perlakuan yaitu akuades sebagai kontrol larutan pengawet, perak nitrat dan ekstrak rebusan daun sirih 250 g l-1, 350 g l-1 dan 450 g l-1 yang diulang sebanyak 4 ulangan dengan 5 sampel tangkai bunga per ulangan. Hasil penelitian menunjukkan bahwa penggunaan berbagai komposisi larutan pengawet tidak berpengaruh nyata dalam memperpanjang umur kesegaran anggrek potong. Kesegaran bunga anggrek Dendrobium ‘Sonia’ dengan penggunaan semua perlakuan larutan pengawet dapat bertahan rata-rata hingga 20 hari. Volume larutan pengawet yang diserap paling besar yaitu penggunaan akuades (7.6 ml). Penggunaan ekstrak rebusan daun sirih konsentrasi 250 g l-1, 350 g l-1 dan 450 g l-1 dengan hasil jumlah bunga terserang cendawan yaitu 2.8, 2.3 dan 2.3 kuntum, hampir separuh jumlahnya dari penggunaan perak nitrat yaitu 5.3 kuntum, menunjukkan adanya kecenderungan positif terhadap penekanan cendawan.

Induksi Multiplikasi Tunas Anthurium Wave of Love (Anthurium plowmanii) secara In Vitro Nurul Khumaida; Riyanti Catrina H S; Dewi Sukma
Jurnal Hortikultura Indonesia Vol. 3 No. 1 (2012): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTAnthurium plowmanii is one of leafy ornamental plants known as anthurium Wave of Love. This plant has a unique characteristic including wave along the leaf side. The aim of this research was to analyze the effect of basic media composition and BAP concentration on in vitro shoot multiplication of Anthurium. Sterile young shoot of Anthurium was used as explant. Three basic media composition including MS, ½MS, and Hyponex and its combination with 0.00 µM BAP, 2.22 µM, 6.66 µM, and 13.32 µM were used as treatments. The result showed that the best medium to induce shoot multiplication were MS + 6.66 µM BAP and ½MS + 13.32 µM BAP which produced 10.4 and 10.3 shoots respectively, higher than other treatments. Medium MS + BAP 13.32 µM decreased the time of shoot initiation. This medium was useful for shoot multiplication of Anthurium plowmanii.Key words: shoot, multiplication, BAP, MS, Hyponex medium, Anthurium ‘Wave of Love’ABSTRAKAnthurium plowmanii adalah tanaman hias daun yang dikenal dengan nama ‘Gelombang Cinta’. Tanaman ini memiliki karakteristik unik, yakni gelombang spanjang tepi daun. Tujuan dari penelitian ini adalah menganalisa pengaruh dari komposisi media dan konsentrasi BAP terhadap multiplikasi tunas Anthurium secara in vitro. Eksplan yang digunakan adalah tunas Anthurium yang sudah disterilisasi. Tiga media dasar yang digunakan meliputi MS, ½ MS, dan Hyponex dan dikombinasikan dengan BAP sebesar 0.00 µM, 2.22 µM, 6.66µM, dan 13.32 µM sebagai perlakuan. Hasil menunjukkan bahwa media terbaik untuk multiplikasi tunas adalah MS + 6.66 µM BAP and ½MS + 13.32 µM BAP yang masing-masing menghasilkan 10.4 dan 10.3 tunas. Hasil tersebut lebih tinggi dibandingkan dengan perlakuan yang lain. Media MS + 13.32 µM BAP mampu mengurangi waktu inisiasi tunas. Media ini dapat berguna untuk multiplikasi tunas Anthurium plowmanii.Kata kunci: tunas, multiplikasi, BAP, MS, Hyponex, Anthurium ‘Gelombang Cinta’

Co-Authors . Giyanto Abdul Hakim Abdullah bin Arif Agus Purwito Aline Sisi Handini Arrin Rosmala Arum Sholikhah Awang Maharijaya Bagas Akmala Putra Budi Marwoto Deden Derajat Matra Dwi Utami Nur Usmani Efendi, Darda Eka Martha Della Rahayu Freta Kirana Balladona Gustaaf Adolf Wattimena Halida Adistya Putri Ilham Nur Ardhi Wicaksono Ismail Maskromo Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muthi’ah Khairun Nisa Nurul Khumaida Nurul Khumaida rawati , Refa Firgiyanto Ritonga, Arya Widura Riyanti Catrina H S Rizal Damanik Roberdi Siberakuno Roni Kartiman Rubiyo Rubiyo Rusmiyati, Henny SANDRA ARIFIN AZIZ Shandra Amarilis Slamet Susanto Sobir Sudarsono Sudarsono Sudarsono Sudarsono, Sudarsono Sulassih Suskandari Kartikaningrum Syamsi Rizal Syarifah Iis Aisyah Tony Liwang Tubagus Kiki Kawakibi Azmi Uchti Nuzul Qhinanti Lubis Ummu Kalsum Ummu Kalsum Waras Nurcholis Yane Riana Putri

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