Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

p-Index From 2019 - 2024

P-Index

This Author published in this journals

All Journal Jurnal Fitopatologi Indonesia

Gede Suastika

Institut Pertanian Bogor

Author-ID : 2451905

Medicine & Pharmacology

Published : 20 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Identifikasi Polerovirus Penyebab Klorosis pada Cabai Asal Bali, Indonesia Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Based on the results of a survey conducted on chilipepper plants in Kertha Village, Payangan Subdistrict, Gianyar District, Bali Province, many chilipepper plants with chlorosis symptoms caused by the virus was observed. Infected plants showed yellowing of the lamina, but the veins remain green. Identification was focused on the Pepper vein yellows virus (PeVYV) through transmission using Aphis nasturtii, observation on the morphology of virus particles, total RNA detection by reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequence. The results showed that the disease can be transmitted from infected plant to healthy plant using A. nasturtii. Viral particle has hexagonal shape with the diameter ~30 nm. RT-PCR of total RNA from severe, moderate, and mild symptomatic infected plants, using specific primer sets for coat protein gene of PeVYV successfully amplified DNA with size of ~650 bp. Coat protein gene sequence of PeVYV isolates Bali, Indonesia showing different chlorosis intensity had the highest homology with PeVYV isolates from Taiwan 95.1–96.6%. Based on the data, PeVYV has been confirmed as the cause of chlorosis in chilipepper plants in Bali, Indonesia.

Identifikasi Nematoda Parasit pada Tanaman Wortel di Dataran Tinggi Malino, Sulawesi Selatan Berdasarkan pada Ciri Morfologi dan Morfometrik Hishar Mirsam; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant-parasitic nematodes are important pests on carrot (Daucus carota) in Malino Highland. This research aimed to identify plant-parasitic nematodes on carrot. The identification was carried out based on the morphological and morphometric characters of second-stage juveniles that were extracted from soil samples. Nematodes were extracted using the flotation-centrifugation technique. Morphometric measurement included body length, stylet length, esophagus length from the basal knob to the esophagus end, tail length from the posterior end to the anus, anterior diameter, maximum body diameter, and posterior diameter. Three genera of plant-parasitic nematodes were identified as Meloidogyne, Rotylenchulus, and Pratylenchus.

Perlakuan Panas Kering dan Bakterisida untuk Menekan Infeksi Pantoea stewartii subsp. stewartii pada Benih Jagung Manis Suswi Nalis; Gede Suastika; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Stewart’s Wilt is an important bacterial disease of sweet corn caused by Pantoea stewartii subsp. stewartii (synonim Erwinia stewartii). This bacteria is a seed transmitted pathogen therefore seed treatment is one method to control stewart’s wilt. The aim of this research was to study the effectiveness of dry heat, bactericide treatment, and their combinations to eliminate P. stewartii subsp. stewartii infection on sweet corn seed without damaging seed quality. The research was conducted in 3 experiments. Experiment I was conducted to determine the treatment window of dry heat and bactericide treatment. The treatment was carried out on sweet corn seed using the P. stewartii subsp. stewartii in vitro. Experiment II was conducted to study dry heat and bactericide treatment on sweet corn seed infested by P. stewartii subsp. stewartii. Experiment III was conducted to study combination of dry heat and bactericide treatment on sweet corn seed infested by P. stewartii subsp. stewartii. The results showed that dry heat treatment at 50 °C for 24 hours was able to eliminate pathogen populations in vitro but was unable to eliminate the 128 pathogen on infected seed (in vivo). Germination tests indicated that seed treatments with dry heat up to 55 °C did not decrease the germination level. The use of bactericide treatment in 100 ppm could reduce the population of bacteria on sweet corn seeds. Bactericide concentration of 150 and 200 ppm could decrease the population of bacteria on sweet corn seeds, however it could cause phytotoxic effect. The combination of bactericide (100 ppm, w/v) ) with dry heat treatment (55 °C for 24 hours) was able to eliminate bacteria on infected seed with seed germination above 85%.

Ekspresi Rekombinan Gen Protein Selubung Pepper vein yellows virus Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Pepper vein yellows virus (PeVYV) isolate from Bali have been identified from pepper plants with chlorosis symptoms. Specific antiserum of PeVYV had not available yet commercially. One of the advance techniques in providing a source of abundant antigen for antiserum production is through molecular approach by overexpressed the coat protein gene in suitable bacterial expression system. PeVYV coat protein gene of ~650 bp in size was amplified using specific primers, then was cloned into pQE30 expression vector and was over expressed in E. coli strain M15 [pREP4]. SDS-PAGE analysis showed that the recombinant coat protein gene of PeVYV was successfully expressed protein band with size of ~25 kDa at 6 hours after induction by 0.5 mM IPTG on 37 °C.

Identifikasi Nematoda Puru Akar, Meloidogyne graminicola, pada Tanaman Padi di Jawa Barat Mochamad Yadi Nurjayadi; Abdul Munif; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root knot nematodes (RKN), Meloidogyne spp. is one of the most important rice pathogen in some regions in the world. Information of RKN on rice plants in West Java is very important because West Java is one of the largest national rice production center. The purpose of this study was to determine and identify the presence of Meloidogyne spp. on rice plants in West Java. Rice plant samples were taken from 7 rice-growing districts in West Java. Typical symptoms of infected rice plants by Meloidogyne spp. are stunting and the formation of root galls. RKN was found to infect rice plants in several areas in Bogor, Cirebon, and Sukabumi. All stadia of the RKN development which include eggs, second, third, and fourth stage jeveniles, adult females and males were found inside the rice root tissues based on nematode staining observation. Morphometric measurements of the body and stylet length, maximum body width, length of the second stage juveniles, and female perineal pattern, indicated that Meloidogyne species found was M. graminicola.

Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa Aprilyani Aprilyani; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root knot nematodes is an important pathogen on potatoes in tropical and sub-tropical areas. Root knot nematodes contribute a significant impact in reducing the quality and quantity of potato tuber. Meloidogyne incognita is one of the species causing the root knot. This research was conducted to identify M. incognita on potatoes in Java Island based on morphological and DNA molecular characteristic. The infected potato tubers with pimple-like knot symptom were collected from Pangalengan (West Java), Banjarnegara (Central Java), and Kota Batu (East Java). Nematode was identified based on morphological character of perineal pattern, and based on molecular DNA character by polymerase chain reaction technique using a pair of specific primer (MI-F and MI-R), followed by DNA fragment sequencing and phylogenetic analysis. Based on morphological character of perineal pattern, M. incognita was detected in all 3 locations; while based on DNA molecular character, and M. incognita was detected in Pangalengan (West Java) and Kota Batu (East Java). M. incognita from Pangalengan had high homology, i.e.99.2% to 99.8% with those isolates from China, India, and Malaysia.

Identifikasi Pepper vein yellows virus yang Berasosiasi dengan Penyakit Yellow Vein Banding pada Tanaman Mentimun di Bali I Dewa Nyoman Nyana; M. Tinny Lestariningsih; Ni Nengah Putri Adnyani; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellowing vein banding disease has been reported infecting cucurbit plants in Bali since 2014. Similar vein banding symptom on chilli pepper was observed previously, and early diagnosis indicated infection of Polerovirus. The objective of this research was to confirm the presence of Polerovirus infection on cucumber plant showing yellow vein banding symptom in Bali. Reverse transcription polymerase chain reaction – based detection method was conducted using specific primer pairs PeVYV-CP-F-BamH1/ PeVYV-CP-R-Pst1followed by sequencing and nucleotide sequence analysis. Specific DNA fragments of ± 650 bp was successfully amplified from field samples. Nucleotide sequence analysis showed that the sequence has the highest similarity > 95% with Pepper vein yellow virus (PeVYV) infecting chili pepper from Indonesia (Bali, and Rembang), Japan, and Greece.

Deteksi dan Evaluasi Keragaman Genetika Candidatus Liberibacter asiaticus sebagai Penyebab Penyakit Huanglongbing di Indonesia Berdasarkan Gen β-operon Muhammad Rizal; Kikin Hamzah Mutaqin; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Huanglongbing also known in Indonesia as citrus vein phloem degeneration (CVPD) is a devastating disease in citrus plantation worldwide, especially in Asia, Africa, and America. In Asian countries including Indonesia, Candidatus Liberibacter asiaticus (CLas) has been confirmed as the causal agent of huanglongbing disease on citrus. Distribution of CLas in Indonesia has been reported in West Borneo, East Nusa Tenggara, Bali, Yogyakarta, Central Java and East Java. The purpose of this study was to detect CLas in several Indonesia’s citrus plantations that has not and has been reported previously and to study its genetic diversity and their relationship. DNA of plant samples, i.e. citrus leaves, was extracted using CTAB method and CLas was amplified using PCR with the A2/J5 primer pair. DNA amplification results showed that infection of CLas was positively detected from samples from Bogor and Cibodas (West Java), Tuban and Jember (East Java), as well as Katung, Bayung Gede, Kerta, and Pancasari (Bali). Alignment of nucleotide sequences from positive samples showed that their ribosomal protein β-operon has high similiarity to that of CLas OK901 originated from Okinawa (Japan). Isolates of CLas originated from Bogor, Cibodas, Tuban, Jember, and Katung have been known to be identical to other CLas of Indonesian origins reported earlier. Isolates of CLas originated from Bayung Gede, Pancasari, and Kerta have single nucleotide polymorphisms at 6 points of bases of the 539 total bases compared in their conservative regions, although only 3 of the 6 bases could affect their amino acid sequences.

Dampak Penyakit Tanaman terhadap Pendapatan Petani Kubis-kubisan di Daerah Agropolitan Kabupaten Cianjur, Jawa Barat Teguh Pratama; Gede Suastika; Ali Nurmansyah
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cruciferous vegetables are very important agricultural commodities for increasing farmers income. The main obstacles in their cultivation involved among others high level of pathogen infestation that may cause reduction in farmer’s income. This research was conducted to determine the main pathogens and their impact on farmer income in Agropolitan area of Cianjur Regency, West Java Province. The research was conducted through three activities, i.e. farmer survey, identification of the main pathogens, and measurement of disease intensity. The results showed that there were three main pathogens on assessed cruciferous vegetables, i.e. Alternaria brassiccicola (alternaria leaf spot), Plasmodiophora brassicae (clubroot), and Xanthomonas campestris (black rot), with disease intensity of 16.7%, 18.7%, and 15.1%, respectively. Clubroot disease was the most affecting disease in decreasing the production of cruciferous vegetables and the farmer income. Incidence of clubroot disease with an average disease intensity of 16.67% might lead to the decrease of farming income about 24%–28%.

Kloning Gen Transcriptional Activator Protein dan Replication-Associated Protein dari Squash leaf curl China virus Penyebab Penyakit Daun Kuning pada Tanaman Mentimun Ni Nengah Putri Adnyani; I Dewa Made Putra Wiratama; I Gede Rai Maya Temaja; Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow disease on Cucurbitaceae caused by Squash leaf curl China virus (SLCCNV; Begomovirus) is an important disease in many countries. Typical yellow leaf curl symptoms was found on cucumber in Tabanan, Bali. The objective of this research is to clone Transcriptional Activator Protein (TrAp) and Replication-Associated Protein (Rep) of SLCCNV Indonesian isolate. Universal primer for Begomovirus, i.e. SPG1/SPG2 was used to amplify DNA fragment of Begomovirus which cover the TrAp and Rep gene. The amplicon of ± 912 bp in size was then cloned into pTZ57R/T plasmid vector by TA-cloning method to obtain plasmid recombinant pTZ-SLCCNV. The plasmid recombinant was successfully confirmed by PCR colony and DNA sequencing. The nucleotide sequences analysis of plasmid recombinant showed highest homology (98%) of the corresponding gene to pumpkin isolate of SLCCNV from Malaysia.

Co-Authors . Supramana Abdul Munif ALI NURMANSYAH Aprilyani Aprilyani Arif Kurniawan Asniwita Asniwita Erniawati Diningsih Giyanto Giyanto Halimah Halimah Hishar Mirsam I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I G. R. M. TEMAJA Kikin H Mutaqin Laras Anjarsari M. Tinny Lestariningsih Mochamad Yadi Nurjayadi Muhamad Taher Muhammad Rizal Ni Nengah Putri Adnyani Rita Kurnia Apindiati Rizki Haerunisa Sari Nurulita SEDYO HARTONO Slamet Susanto SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Suswi Nalis Teguh Pratama Tomohide Natsuaki TRI ASMIRA DAMAYANTI Zalzilatul Hikmia Hikmia

Title

Found 20 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 20 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 20 Documents
Search